RchiOBHm_Chr5g0002241

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015757
Physical Location & Seq
Forward (+)
1291473 .. 1293957
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28362

Sequence Viewer

Length: 1041 bp
ATGTCCCGAATCTCGGAATCCGGCCAGGTCCCCAAACCGGCGAAGCCTCGAAGGACAGACGAGATCCCAACTCATGGCACTACCATGATTTTTATAAAGACGAATGAACTGGTAATCGTTGGGAGCGACTCTAGAATTAGCAACGGCAGATCGAACCGTATAGTTAACGATGATGTGTTTAAAATTGAAGCAATAACTTCAAATATGTATGCAACATGTACGGGATTGTTTCGTTCAGGCCTAGGATTGTTTGAAGATTTGCGAAAGCAGTACATGGAGAAGAAAAACTCTGGTGTTGAACCGACTCTTAAAGAAATTGCAGATTACGGTCGGCAACGTATCCGGACATGGGACAATGACGCGCATTATAGTGTTATTGGAATTGAAAATGGGAAGCCCCAAGCTTATGGTTTTACTGATAAAGAAAAGTACAAGGATACTGTGGCTCTCAAGACATGGTGGTCATTTGGCAGTGGTGGAAAGTATGCTAAGCCAAAACTTAGAGCTGATTACAATAGGGGGATGTCTGAACCACTTGCATTGAAGTTAGCTGAAACGACTTTTTTAGCTGCAGCCTTAGGTGATCCATTTACCGGGGGATACCTACGTGTATTTGTCTTTGACTCAAACGGAAAAAGAAAGGAGTATTCCAAGACTACATTGGAGTTGTATTTGAGTTACTACTCACTCTCAGATGAGTTCCATTCAAGAACAATCTACATCTTTTCTCGCGGCCAGATTGGTGATACTGTAGTGACCCAATGGTTTAGTTCTGGATGTCTTATTGAACCGGTTGAGGTGATGGTCGTACACCCTGTTGGATATATTGGGGGATATATTGTTCGTCGTGTGGTATTTCAGGAGAAAGATGCTGCAACCATTATTCAAGAAGTACAACACCAATGTGAAAGATATGGTTGTCGTGATAGAACTGACGAAGAAATTAATACGTTTCCGTGTCTTCAAATTGTTAATGAGCGATTTCATTTTGCAAAGTTAACAAAAGAGCAATTGAGCAATTTACTTGGACAGAATGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

346

Amino Acids

39.3

Weight (kDa)

8.61

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 25 - 205 9.5e-13 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 95
AasI GACNNNNNNGTC 1 cut(s) 460
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 2 cut(s) 362, 732
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 1 cut(s) 732
AclWI GGATC 2 cut(s) 58, 578
AcoI YGGCCR 2 cut(s) 22, 733
AfaI GTAC 5 cut(s) 220, 272, 431, 810, 894
AfiI CCNNNNNNNGG 5 cut(s) 13, 37, 74, 349, 593
AflIII ACRYGT 2 cut(s) 215, 607
AgeI ACCGGT 1 cut(s) 790
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 1 cut(s) 903
AloI GAACNNNNNNTCC 2 cut(s) 825, 857
AluBI AGCT 4 cut(s) 404, 506, 551, 569
AluI AGCT 4 cut(s) 404, 506, 551, 569
AlwI GGATC 2 cut(s) 58, 578
Aor13HI TCCGGA 1 cut(s) 342
AoxI GGCC 3 cut(s) 22, 238, 733
ApeKI GCWGC 3 cut(s) 569, 572, 872
AseI ATTAAT 1 cut(s) 945
AsiGI ACCGGT 1 cut(s) 790
AspA2I CCTAGG 1 cut(s) 241
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 3 cut(s) 593, 755, 811
AvaII GGWCC 1 cut(s) 28
AvrII CCTAGG 1 cut(s) 241
AxyI CCTNAGG 1 cut(s) 577
BaeI ACNNNNGTAYC 4 cut(s) 592, 625, 738, 771
BbsI GAAGAC 1 cut(s) 953
BbvI GCAGC 3 cut(s) 556, 584, 859
BccI CCATC 1 cut(s) 796
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 26
BciVI GTATCC 3 cut(s) 350, 430, 593
BcnI CCSGG 1 cut(s) 595
BfaI CTAG 2 cut(s) 132, 242
BfmI CTRYAG 2 cut(s) 570, 750
BfuI GTATCC 3 cut(s) 350, 430, 593
BisI GCNGC 4 cut(s) 570, 573, 733, 873
BlnI CCTAGG 1 cut(s) 241
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 4 cut(s) 571, 574, 734, 874
Bme1390I CCNGG 2 cut(s) 26, 595
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 26, 595
BmsI GCATC 1 cut(s) 859
BpiI GAAGAC 1 cut(s) 953
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 608
BsaJI CCNNGG 2 cut(s) 241, 594
BsaWI WCCGGW 2 cut(s) 342, 790
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 37, 74, 349, 593
Bse118I RCCGGY 2 cut(s) 37, 790
Bse1I ACTGG 1 cut(s) 114
Bse21I CCTNAGG 1 cut(s) 577
BseAI TCCGGA 1 cut(s) 342
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 241, 594
BseGI GGATG 2 cut(s) 528, 782
BseLI CCNNNNNNNGG 5 cut(s) 13, 37, 74, 349, 593
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 3 cut(s) 556, 584, 859
Bsh1236I CGCG 2 cut(s) 362, 732
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 3 cut(s) 24, 240, 735
BshTI ACCGGT 1 cut(s) 790
BsiEI CGRYCG 1 cut(s) 331
BsiSI CCGG 5 cut(s) 21, 38, 343, 594, 791
BslFI GGGAC 2 cut(s) 14, 365
BslI CCNNNNNNNGG 5 cut(s) 13, 37, 74, 349, 593
BsmFI GGGAC 2 cut(s) 14, 365
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 3 cut(s) 24, 240, 735
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 3 cut(s) 63, 149, 583
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 1 cut(s) 732
BspANI GGCC 3 cut(s) 24, 240, 735
BspCNI CTCAG 1 cut(s) 704
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 2 cut(s) 362, 732
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 2 cut(s) 58, 578
BsrFI RCCGGY 2 cut(s) 37, 790
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 2 cut(s) 37, 790
BssECI CCNNGG 2 cut(s) 241, 594
BssMI GATC 3 cut(s) 63, 149, 583
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 4 cut(s) 158, 329, 442, 751
BstBAI YACGTR 1 cut(s) 608
BstDEI CTNAG 4 cut(s) 489, 500, 577, 691
BstF5I GGATG 2 cut(s) 528, 782
BstFNI CGCG 2 cut(s) 362, 732
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 3 cut(s) 66, 152, 586
BstMBI GATC 3 cut(s) 63, 149, 583
BstMCI CGRYCG 1 cut(s) 331
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 2 cut(s) 24, 593
BstSFI CTRYAG 2 cut(s) 570, 750
BstUI CGCG 2 cut(s) 362, 732
BstV1I GCAGC 3 cut(s) 556, 584, 859
BstV2I GAAGAC 1 cut(s) 953
BstX2I RGATCY 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 407
BstYI RGATCY 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 577
BsuI GTATCC 3 cut(s) 350, 430, 593
BsuRI GGCC 3 cut(s) 24, 240, 735
BtsCI GGATG 2 cut(s) 528, 782
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 1 cut(s) 478
CfoI GCGC 1 cut(s) 364
Cfr10I RCCGGY 2 cut(s) 37, 790
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 368
Csp6I GTAC 5 cut(s) 219, 271, 430, 809, 893
CspAI ACCGGT 1 cut(s) 790
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviAII CATG 6 cut(s) 74, 85, 216, 274, 348, 456
CviQI GTAC 5 cut(s) 219, 271, 430, 809, 893
DdeI CTNAG 4 cut(s) 489, 500, 577, 691
DpnI GATC 3 cut(s) 65, 151, 585
DpnII GATC 3 cut(s) 63, 149, 583
DraI TTTAAA 1 cut(s) 181
DrdI GACNNNNNNGTC 1 cut(s) 460
DseDI GACNNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 2 cut(s) 22, 733
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 28
Eco81I CCTNAGG 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 6 cut(s) 77, 88, 219, 277, 351, 459
FaqI GGGAC 2 cut(s) 14, 365
FatI CATG 6 cut(s) 73, 84, 215, 273, 347, 455
Fnu4HI GCNGC 4 cut(s) 570, 573, 733, 873
FokI GGATG 2 cut(s) 535, 789
Fsp4HI GCNGC 4 cut(s) 570, 573, 733, 873
FspBI CTAG 2 cut(s) 132, 242
GlaI GCGC 1 cut(s) 363
GluI GCNGC 4 cut(s) 570, 573, 733, 873
HaeIII GGCC 3 cut(s) 24, 240, 735
HapII CCGG 5 cut(s) 21, 38, 343, 594, 791
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 364
Hin1II CATG 6 cut(s) 77, 88, 219, 277, 351, 459
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 2 cut(s) 166, 999
HindII GTYRAC 2 cut(s) 166, 999
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 5 cut(s) 9, 17, 128, 304, 623
HpaI GTTAAC 2 cut(s) 166, 999
HpaII CCGG 5 cut(s) 21, 38, 343, 594, 791
HphI GGTGA 3 cut(s) 593, 755, 811
Hpy166II GTNNAC 3 cut(s) 166, 811, 999
Hpy188I TCNGA 3 cut(s) 16, 529, 694
Hpy188III TCNNGA 9 cut(s) 6, 132, 343, 451, 708, 774, 860, 887, 923
Hpy8I GTNNAC 3 cut(s) 166, 811, 999
Hpy99I CGWCG 1 cut(s) 849
HpyAV CCTTC 1 cut(s) 45
HpyCH4III ACNGT 4 cut(s) 158, 329, 442, 751
HpyCH4IV ACGT 3 cut(s) 337, 607, 950
HpyCH4V TGCA 6 cut(s) 212, 320, 539, 572, 875, 992
HpyF3I CTNAG 4 cut(s) 489, 500, 577, 691
HpySE526I ACGT 3 cut(s) 337, 607, 950
Hsp92II CATG 6 cut(s) 77, 88, 219, 277, 351, 459
HspAI GCGC 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 342
KspAI GTTAAC 2 cut(s) 166, 999
Kzo9I GATC 3 cut(s) 63, 149, 583
LmnI GCTCC 1 cut(s) 123
Lsp1109I GCAGC 3 cut(s) 556, 584, 859
LweI GCATC 1 cut(s) 859
MaeI CTAG 2 cut(s) 132, 242
MaeII ACGT 3 cut(s) 337, 607, 950
MaeIII GTNAC 2 cut(s) 677, 754
MalI GATC 3 cut(s) 65, 151, 585
MboI GATC 3 cut(s) 63, 149, 583
MboII GAAGA 4 cut(s) 266, 292, 950, 953
MfeI CAATTG 1 cut(s) 1010
MflI RGATCY 1 cut(s) 63
MluCI AATT 8 cut(s) 135, 183, 315, 381, 942, 966, 1010, 1018
MlyI GAGTC 3 cut(s) 122, 298, 617
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 2 cut(s) 57, 790
MroI TCCGGA 1 cut(s) 342
MseI TTAA 6 cut(s) 165, 180, 309, 945, 972, 998
MslI CAYNNNNRTG 3 cut(s) 83, 369, 903
MspI CCGG 5 cut(s) 21, 38, 343, 594, 791
MspR9I CCNGG 2 cut(s) 26, 595
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 1 cut(s) 1039
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 2 cut(s) 362, 732
NciI CCSGG 1 cut(s) 595
NdeII GATC 3 cut(s) 63, 149, 583
NlaIII CATG 6 cut(s) 77, 88, 219, 277, 351, 459
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 754
NspI RCATGY 1 cut(s) 219
OliI CACNNNNGTG 1 cut(s) 903
PceI AGGCCT 1 cut(s) 240
PciI ACATGT 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 1039
PfeI GAWTC 2 cut(s) 9, 17
PflMI CCANNNNNTGG 1 cut(s) 74
PinAI ACCGGT 1 cut(s) 790
PkrI GCNGC 4 cut(s) 571, 574, 734, 874
PleI GAGTC 3 cut(s) 122, 298, 617
PpsI GAGTC 3 cut(s) 122, 298, 617
Ppu21I YACGTR 1 cut(s) 608
PpuMI RGGWCCY 1 cut(s) 28
PscI ACATGT 1 cut(s) 215
PshBI ATTAAT 1 cut(s) 945
PsiI TTATAA 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 28
PspPPI RGGWCCY 1 cut(s) 28
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 5 cut(s) 220, 272, 431, 810, 894
RsaNI GTAC 5 cut(s) 219, 271, 430, 809, 893
RseI CAYNNNNRTG 3 cut(s) 83, 369, 903
SaqAI TTAA 6 cut(s) 165, 180, 309, 945, 972, 998
SatI GCNGC 4 cut(s) 570, 573, 733, 873
Sau3AI GATC 3 cut(s) 63, 149, 583
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 3 cut(s) 122, 298, 617
ScrFI CCNGG 2 cut(s) 26, 595
SfaNI GCATC 1 cut(s) 859
SfcI CTRYAG 2 cut(s) 570, 750
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 3 cut(s) 83, 369, 903
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 8 cut(s) 135, 183, 315, 381, 942, 966, 1010, 1018
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 1 cut(s) 732
SspMI CTAG 2 cut(s) 132, 242
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 24, 593
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 4 cut(s) 158, 329, 442, 751
TaiI ACGT 3 cut(s) 340, 610, 953
TaqI TCGA 2 cut(s) 49, 152
TasI AATT 8 cut(s) 135, 183, 315, 381, 942, 966, 1010, 1018
TatI WGTACW 3 cut(s) 270, 429, 892
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 2 cut(s) 9, 17
Tru1I TTAA 6 cut(s) 165, 180, 309, 945, 972, 998
Tru9I TTAA 6 cut(s) 165, 180, 309, 945, 972, 998
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 3 cut(s) 569, 572, 872
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 2 cut(s) 120, 974
TspGWI ACGGA 2 cut(s) 645, 945
TspRI CASTG 1 cut(s) 478
Van91I CCANNNNNTGG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 1 cut(s) 945
XbaI TCTAGA 1 cut(s) 131
XceI RCATGY 1 cut(s) 219
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XmaJI CCTAGG 1 cut(s) 241
XspI CTAG 2 cut(s) 132, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.