RLG00000031011

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015757
Physical Location & Seq
Forward (+)
1342885 .. 1345013
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031011

Sequence Viewer

Length: 1035 bp
ATGTCCCGAATCTGGGAATCCGGCCAGGTCCCCAAACCGGCGAAGCCTCGAAGGACAGACGAGATCCCAACTCATGGCACTACCATGATTTTTATAAAGACGAATGAACTGGTGATCGTTGGCAGCGACTCTAGATGTAGCAACGGCAGATCGAACCTTAACGATGATGTGTTTAAAATTGAAGCAATAACTTCAAATATGTATGCAACATGTACGGGATTGTTTAGTGTAGGCCTAGGATTGTTTGAAGATTTGCGAAAGCAGTACATGGAGAAGAAAAACTCTGGTGTTGAACCGACTCTTAAAGAAATTGCAGATTACGGTCGGCAACATATCCGGACATGGAACAATGACGCGCATTATAATGTTATTGGAATTGAAAATGGGAAGCCCCAAGCTTATGGTTTTACTGATAAAGAAAAGTACAAGGATAGTGTGGCTCTCAAGACATGGTGGTCATTTGGCAGTGGTGGGAAGTATGCTAAGCCAAAACTTAGAGCTGATTACAAGAAGGGGATGTCTGAACCACTTGCATTGAAGTTAGCTGAAACGACTTTTTTAGCTGCAGCCTTAGGTGATCCATTTACCGGGGGATACCTACGTGTATTTGTCTTTGACTCCAACGGAAAAAGAAAGGAGTATTCCAAGACTACATTGGAGTTGTATTTGAGTTACTACTCACTCTCAGATGAGTTCCATTCAAGAACAATCTACATCTTTTCTCGCGGCCAGATTGGTGACACTGTAGTGACCCAATGGTTTAGTTCTGGATGTCTTATTGAACCGGTTGAGGTGATGGTCGTACACCCTGTTGGATATATTGGGGGATATATTGTTCGTCGTGTGGTATTTCAGGAGAAAGATGCTGCAGCCATTATTCAAGAAGTACAACACCAATGTGAAAGATATGGTTGTCGTGATAGAACTGATGAAGAAATTAATACGTTTCCGTGTCTTCAAATTGTTAATGAGCGATTTCATTTTGCAAAGTTAACAAAAGAGCAATTGAGCAATTTACTTGGACAGAATGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

345

Amino Acids

39.02

Weight (kDa)

8.36

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 25 - 203 2.2e-14 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 95, 363
AasI GACNNNNNNGTC 1 cut(s) 454
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 2 cut(s) 356, 726
AccIII TCCGGA 1 cut(s) 336
AciI CCGC 1 cut(s) 726
AclWI GGATC 2 cut(s) 58, 572
AcoI YGGCCR 2 cut(s) 22, 727
AfaI GTAC 5 cut(s) 214, 266, 425, 804, 888
AfiI CCNNNNNNNGG 5 cut(s) 12, 13, 37, 74, 587
AflIII ACRYGT 2 cut(s) 209, 601
AgeI ACCGGT 1 cut(s) 784
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 2 cut(s) 746, 897
AloI GAACNNNNNNTCC 2 cut(s) 819, 851
AluBI AGCT 4 cut(s) 398, 500, 545, 563
AluI AGCT 4 cut(s) 398, 500, 545, 563
AlwI GGATC 2 cut(s) 58, 572
Aor13HI TCCGGA 1 cut(s) 336
AoxI GGCC 3 cut(s) 22, 232, 727
ApeKI GCWGC 5 cut(s) 123, 563, 566, 866, 869
AseI ATTAAT 1 cut(s) 939
AsiGI ACCGGT 1 cut(s) 784
AspA2I CCTAGG 1 cut(s) 235
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 4 cut(s) 124, 587, 749, 805
AvaII GGWCC 1 cut(s) 28
AvrII CCTAGG 1 cut(s) 235
AxyI CCTNAGG 1 cut(s) 571
BaeI ACNNNNGTAYC 2 cut(s) 586, 619
BbsI GAAGAC 1 cut(s) 947
BbvI GCAGC 5 cut(s) 135, 550, 578, 853, 881
BccI CCATC 1 cut(s) 790
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 26
BciVI GTATCC 1 cut(s) 587
BcnI CCSGG 1 cut(s) 589
BfaI CTAG 2 cut(s) 132, 236
BfmI CTRYAG 3 cut(s) 564, 744, 867
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 6 cut(s) 124, 564, 567, 727, 867, 870
BlnI CCTAGG 1 cut(s) 235
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 6 cut(s) 125, 565, 568, 728, 868, 871
Bme1390I CCNGG 2 cut(s) 26, 589
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 26, 589
BmsI GCATC 1 cut(s) 853
BpiI GAAGAC 1 cut(s) 947
Bpu1102I GCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 589
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 235, 588
BsaWI WCCGGW 2 cut(s) 336, 784
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 13, 37, 74, 587
Bse118I RCCGGY 2 cut(s) 37, 784
Bse1I ACTGG 1 cut(s) 114
Bse21I CCTNAGG 1 cut(s) 571
BseAI TCCGGA 1 cut(s) 336
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 235, 588
BseGI GGATG 2 cut(s) 522, 776
BseLI CCNNNNNNNGG 5 cut(s) 12, 13, 37, 74, 587
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 5 cut(s) 135, 550, 578, 853, 881
Bsh1236I CGCG 2 cut(s) 356, 726
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 3 cut(s) 24, 234, 729
BshTI ACCGGT 1 cut(s) 784
BsiEI CGRYCG 1 cut(s) 325
BsiSI CCGG 5 cut(s) 21, 38, 337, 588, 785
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 5 cut(s) 12, 13, 37, 74, 587
BsmFI GGGAC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 3 cut(s) 24, 234, 729
Bsp13I TCCGGA 1 cut(s) 336
Bsp143I GATC 4 cut(s) 63, 114, 149, 577
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 1 cut(s) 726
BspANI GGCC 3 cut(s) 24, 234, 729
BspCNI CTCAG 1 cut(s) 698
BspEI TCCGGA 1 cut(s) 336
BspFNI CGCG 2 cut(s) 356, 726
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 2 cut(s) 568, 871
BspPI GGATC 2 cut(s) 58, 572
BsrFI RCCGGY 2 cut(s) 37, 784
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 2 cut(s) 37, 784
BssECI CCNNGG 2 cut(s) 235, 588
BssMI GATC 4 cut(s) 63, 114, 149, 577
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 323, 745
BstBAI YACGTR 1 cut(s) 602
BstDEI CTNAG 4 cut(s) 483, 494, 571, 685
BstF5I GGATG 2 cut(s) 522, 776
BstFNI CGCG 2 cut(s) 356, 726
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 4 cut(s) 66, 117, 152, 580
BstMBI GATC 4 cut(s) 63, 114, 149, 577
BstMCI CGRYCG 1 cut(s) 325
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 2 cut(s) 24, 587
BstSFI CTRYAG 3 cut(s) 564, 744, 867
BstUI CGCG 2 cut(s) 356, 726
BstV1I GCAGC 5 cut(s) 135, 550, 578, 853, 881
BstV2I GAAGAC 1 cut(s) 947
BstX2I RGATCY 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 571
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 3 cut(s) 24, 234, 729
BtsCI GGATG 2 cut(s) 522, 776
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 472, 741
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 2 cut(s) 37, 784
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 362
Csp6I GTAC 5 cut(s) 213, 265, 424, 803, 887
CspAI ACCGGT 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 6 cut(s) 74, 85, 210, 268, 342, 450
CviQI GTAC 5 cut(s) 213, 265, 424, 803, 887
DdeI CTNAG 4 cut(s) 483, 494, 571, 685
DpnI GATC 4 cut(s) 65, 116, 151, 579
DpnII GATC 4 cut(s) 63, 114, 149, 577
DraI TTTAAA 1 cut(s) 175
DrdI GACNNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 454
EaeI YGGCCR 2 cut(s) 22, 727
Eco130I CCWWGG 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 28
Eco81I CCTNAGG 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 6 cut(s) 77, 88, 213, 271, 345, 453
FaqI GGGAC 1 cut(s) 14
FatI CATG 6 cut(s) 73, 84, 209, 267, 341, 449
Fnu4HI GCNGC 6 cut(s) 124, 564, 567, 727, 867, 870
FokI GGATG 2 cut(s) 529, 783
Fsp4HI GCNGC 6 cut(s) 124, 564, 567, 727, 867, 870
FspBI CTAG 2 cut(s) 132, 236
GlaI GCGC 1 cut(s) 357
GluI GCNGC 6 cut(s) 124, 564, 567, 727, 867, 870
HaeIII GGCC 3 cut(s) 24, 234, 729
HapII CCGG 5 cut(s) 21, 38, 337, 588, 785
HgaI GACGC 1 cut(s) 362
HhaI GCGC 1 cut(s) 358
Hin1II CATG 6 cut(s) 77, 88, 213, 271, 345, 453
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 993
HindII GTYRAC 1 cut(s) 993
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 5 cut(s) 9, 17, 128, 298, 617
HpaI GTTAAC 1 cut(s) 993
HpaII CCGG 5 cut(s) 21, 38, 337, 588, 785
HphI GGTGA 4 cut(s) 124, 587, 749, 805
Hpy166II GTNNAC 2 cut(s) 805, 993
Hpy188I TCNGA 2 cut(s) 523, 688
Hpy188III TCNNGA 9 cut(s) 6, 132, 337, 445, 702, 768, 854, 881, 917
Hpy8I GTNNAC 2 cut(s) 805, 993
Hpy99I CGWCG 1 cut(s) 843
HpyAV CCTTC 2 cut(s) 45, 505
HpyCH4III ACNGT 2 cut(s) 323, 745
HpyCH4IV ACGT 2 cut(s) 601, 944
HpyCH4V TGCA 6 cut(s) 206, 314, 533, 566, 869, 986
HpyF3I CTNAG 4 cut(s) 483, 494, 571, 685
HpySE526I ACGT 2 cut(s) 601, 944
Hsp92II CATG 6 cut(s) 77, 88, 213, 271, 345, 453
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 336
KspAI GTTAAC 1 cut(s) 993
Kzo9I GATC 4 cut(s) 63, 114, 149, 577
Lsp1109I GCAGC 5 cut(s) 135, 550, 578, 853, 881
LweI GCATC 1 cut(s) 853
MaeI CTAG 2 cut(s) 132, 236
MaeII ACGT 2 cut(s) 601, 944
MaeIII GTNAC 3 cut(s) 671, 737, 748
MalI GATC 4 cut(s) 65, 116, 151, 579
MboI GATC 4 cut(s) 63, 114, 149, 577
MboII GAAGA 4 cut(s) 260, 286, 944, 947
MfeI CAATTG 1 cut(s) 1004
MflI RGATCY 1 cut(s) 63
MluCI AATT 7 cut(s) 177, 309, 375, 936, 960, 1004, 1012
MlyI GAGTC 3 cut(s) 122, 292, 611
MmeI TCCRAC 2 cut(s) 645, 793
MnlI CCTC 2 cut(s) 57, 784
MroI TCCGGA 1 cut(s) 336
MseI TTAA 6 cut(s) 159, 174, 303, 939, 966, 992
MslI CAYNNNNRTG 4 cut(s) 83, 363, 746, 897
MspI CCGG 5 cut(s) 21, 38, 337, 588, 785
MspR9I CCNGG 2 cut(s) 26, 589
MunI CAATTG 1 cut(s) 1004
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 2 cut(s) 356, 726
NciI CCSGG 1 cut(s) 589
NdeII GATC 4 cut(s) 63, 114, 149, 577
NlaIII CATG 6 cut(s) 77, 88, 213, 271, 345, 453
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 737, 748
NspI RCATGY 1 cut(s) 213
OliI CACNNNNGTG 2 cut(s) 746, 897
PceI AGGCCT 1 cut(s) 234
PciI ACATGT 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 1033
PfeI GAWTC 2 cut(s) 9, 17
PflMI CCANNNNNTGG 1 cut(s) 74
PinAI ACCGGT 1 cut(s) 784
PkrI GCNGC 6 cut(s) 125, 565, 568, 728, 868, 871
PleI GAGTC 3 cut(s) 122, 292, 611
PpsI GAGTC 3 cut(s) 122, 292, 611
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 28
PscI ACATGT 1 cut(s) 209
PshBI ATTAAT 1 cut(s) 939
PsiI TTATAA 2 cut(s) 95, 363
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 28
PspPPI RGGWCCY 1 cut(s) 28
PstI CTGCAG 2 cut(s) 568, 871
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 5 cut(s) 214, 266, 425, 804, 888
RsaNI GTAC 5 cut(s) 213, 265, 424, 803, 887
RseI CAYNNNNRTG 4 cut(s) 83, 363, 746, 897
SaqAI TTAA 6 cut(s) 159, 174, 303, 939, 966, 992
SatI GCNGC 6 cut(s) 124, 564, 567, 727, 867, 870
Sau3AI GATC 4 cut(s) 63, 114, 149, 577
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 3 cut(s) 122, 292, 611
ScrFI CCNGG 2 cut(s) 26, 589
SfaNI GCATC 1 cut(s) 853
SfcI CTRYAG 3 cut(s) 564, 744, 867
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 4 cut(s) 83, 363, 746, 897
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 7 cut(s) 177, 309, 375, 936, 960, 1004, 1012
SseBI AGGCCT 1 cut(s) 234
SsiI CCGC 1 cut(s) 726
SspMI CTAG 2 cut(s) 132, 236
StuI AGGCCT 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 24, 587
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 2 cut(s) 323, 745
TaiI ACGT 2 cut(s) 604, 947
TaqI TCGA 2 cut(s) 49, 152
TasI AATT 7 cut(s) 177, 309, 375, 936, 960, 1004, 1012
TatI WGTACW 3 cut(s) 264, 423, 886
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 2 cut(s) 9, 17
Tru1I TTAA 6 cut(s) 159, 174, 303, 939, 966, 992
Tru9I TTAA 6 cut(s) 159, 174, 303, 939, 966, 992
TscAI CASTG 2 cut(s) 472, 748
TseFI GTSAC 2 cut(s) 737, 748
TseI GCWGC 5 cut(s) 123, 563, 566, 866, 869
Tsp45I GTSAC 2 cut(s) 737, 748
TspDTI ATGAA 3 cut(s) 120, 945, 968
TspGWI ACGGA 2 cut(s) 639, 939
TspRI CASTG 2 cut(s) 472, 748
Van91I CCANNNNNTGG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 1 cut(s) 939
XbaI TCTAGA 1 cut(s) 131
XceI RCATGY 1 cut(s) 213
XcmI CCANNNNNNNNNTGG 1 cut(s) 652
XmaJI CCTAGG 1 cut(s) 235
XspI CTAG 2 cut(s) 132, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.