Rroxscaffold_2G00125930

threonine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015757
Physical Location & Seq
Reverse (-)
60975250 .. 60977526
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125930.1

Sequence Viewer

Length: 1044 bp
ATGTCCCGAATCTCGGAATCCGGCCAGGTCCCCAAACCGGCGAAGCCTCGAAGGAAAGACGAGATCCCAACTCATGGCACTACCACGATTTTTATAAAGACGAATGAACTGGTGATCGTTGGCAGCGACTCTAGATGTAGCAACGGCAGATCGAACCGTATAGTTAACGATGATGTGTTTAAAATTGAAGCAATAACTTCAAAAAATATGTATGCAACATGTACGGGATTGTTTAGTGCAGGCCTAGGATTGTTTGAAGATTTGCGAAAGCAGTACATGGAGAAGAAAAACTCTGGTGTTGAACCGACTCTTAAAGAAATTGCAGATTACGGTCGTCAACGTATCCGGACATGGAACAATGCCGCGCATTATAATGTTATTGGAATTGAAAATGGGAAGCCCCAAGCTTATGGTTTTACTGATAAAGAAAAGTACAAGGATAGTGTGCCTCTCAAGACATGGTGGTCATTTGGCAGTGGTGGAACGTATGCTAAGCCAAAACTTAGAGCTGATTACAATAGGGGGATGTCTGAACCACTTGCATTGAAGTTAGCTGAAACGGCTTTCTTAGCTGCAGCCTTAGGTGATCCATTTACCGGGGGATACCTACGTGTATTTGTCTTTGACTCAAACGGATTAAGAAGGGAGTATTCCAAGACTACATTGGAGTTGTATTTGAGTTACTACTCACTCTCAGATGAGTTCCATTCAAGAACAATCTACATCTTTTCTCGCGGCCAGATTGGTGACACTGTAGTGACCCAATGGTTTAGTTCTGGATGTCTTATTGAACCGGTTGAGGTGATGGTTGTACACCCTGTTGGATATATTGGAGGATATATTGTTCGTCGTGTGGTTTTTCAGGAGAAAGATGCTGCAACCATTATTCAAGAAGTACAACACCAATGTGAAAGACATGGTTGTCGTGATAGAATTGACGAAGAAATTAATACGTTTCCATGTCTTCAAATTGTTAATGAGCGATTTTATTTTGCAAAGTTAACAAAAGAGCAATTGAGCAATTTACTTGGACAGAATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0001775 GO:0002376 GO:0002520 GO:0002521 GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0005839 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010498 GO:0010499 GO:0012505 GO:0016020 GO:0016043 GO:0016579 GO:0016787 GO:0019221 GO:0019222 GO:0019538 GO:0019774 GO:0019882 GO:0019941 GO:0022607 GO:0023052 GO:0030097 GO:0030098 GO:0030154 GO:0030162 GO:0030163 GO:0030217 GO:0031597 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034097 GO:0034340 GO:0034515 GO:0034622 GO:0036037 GO:0036211 GO:0042110 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043374 GO:0043412 GO:0043632 GO:0043687 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0045321 GO:0045444 GO:0046631 GO:0046632 GO:0046649 GO:0048513 GO:0048534 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051336 GO:0051603 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060337 GO:0065003 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070646 GO:0070647 GO:0070887 GO:0071310 GO:0071345 GO:0071357 GO:0071704 GO:0071840 GO:0080090 GO:0080129 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369 GO:1990111
KEGG Pathways
Metabolic & Signaling
ko02010 ko03050 ko04145 ko04612 ko05168 ko05340 map02010 map03050 map04145 map04612 map05168 map05340
Pfam Domains
Protein Families
Proteasome

Protein Analysis

347

Amino Acids

39.29

Weight (kDa)

8.72

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 25 - 206 4.9e-13 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 95, 372
AasI GACNNNNNNGTC 2 cut(s) 463, 921
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 2 cut(s) 365, 735
AccIII TCCGGA 1 cut(s) 345
AciI CCGC 2 cut(s) 363, 735
AclWI GGATC 2 cut(s) 58, 581
AcoI YGGCCR 2 cut(s) 22, 736
AfaI GTAC 5 cut(s) 223, 275, 434, 813, 897
AfiI CCNNNNNNNGG 4 cut(s) 13, 37, 74, 596
AflIII ACRYGT 2 cut(s) 218, 610
AgeI ACCGGT 1 cut(s) 793
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 2 cut(s) 755, 906
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
AluBI AGCT 4 cut(s) 407, 509, 554, 572
AluI AGCT 4 cut(s) 407, 509, 554, 572
AlwI GGATC 2 cut(s) 58, 581
Aor13HI TCCGGA 1 cut(s) 345
AoxI GGCC 3 cut(s) 22, 241, 736
ApeKI GCWGC 4 cut(s) 123, 572, 575, 875
AseI ATTAAT 1 cut(s) 948
AsiGI ACCGGT 1 cut(s) 793
AspA2I CCTAGG 1 cut(s) 244
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 4 cut(s) 124, 596, 758, 814
AvaII GGWCC 1 cut(s) 28
AvrII CCTAGG 1 cut(s) 244
AxyI CCTNAGG 1 cut(s) 580
BaeI ACNNNNGTAYC 2 cut(s) 595, 628
BbsI GAAGAC 1 cut(s) 956
BbvI GCAGC 4 cut(s) 135, 559, 587, 862
BccI CCATC 1 cut(s) 799
BceAI ACGGC 2 cut(s) 160, 576
BciT130I CCWGG 1 cut(s) 26
BciVI GTATCC 2 cut(s) 353, 596
BcnI CCSGG 1 cut(s) 598
BfaI CTAG 3 cut(s) 132, 245, 1042
BfmI CTRYAG 2 cut(s) 573, 753
BfuI GTATCC 2 cut(s) 353, 596
BisI GCNGC 6 cut(s) 124, 363, 573, 576, 736, 876
BlnI CCTAGG 1 cut(s) 244
BlpI GCTNAGC 1 cut(s) 492
BlsI GCNGC 6 cut(s) 125, 364, 574, 577, 737, 877
Bme1390I CCNGG 2 cut(s) 26, 598
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 26, 598
BmsI GCATC 1 cut(s) 862
BpiI GAAGAC 1 cut(s) 956
Bpu1102I GCTNAGC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 598
BsaAI YACGTR 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 244, 597
BsaWI WCCGGW 2 cut(s) 345, 793
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 37, 74, 596
Bse118I RCCGGY 2 cut(s) 37, 793
Bse1I ACTGG 1 cut(s) 114
Bse21I CCTNAGG 1 cut(s) 580
BseAI TCCGGA 1 cut(s) 345
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 244, 597
BseGI GGATG 2 cut(s) 531, 785
BseLI CCNNNNNNNGG 4 cut(s) 13, 37, 74, 596
BseMII CTCAG 1 cut(s) 708
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 4 cut(s) 135, 559, 587, 862
BsgI GTGCAG 1 cut(s) 258
Bsh1236I CGCG 2 cut(s) 365, 735
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 3 cut(s) 24, 243, 738
BshTI ACCGGT 1 cut(s) 793
BsiEI CGRYCG 1 cut(s) 334
BsiSI CCGG 5 cut(s) 21, 38, 346, 597, 794
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 4 cut(s) 13, 37, 74, 596
BsmFI GGGAC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 3 cut(s) 24, 243, 738
Bsp13I TCCGGA 1 cut(s) 345
Bsp1407I TGTACA 1 cut(s) 811
Bsp143I GATC 4 cut(s) 63, 114, 149, 586
Bsp1720I GCTNAGC 1 cut(s) 492
BspACI CCGC 2 cut(s) 363, 735
BspANI GGCC 3 cut(s) 24, 243, 738
BspCNI CTCAG 1 cut(s) 707
BspEI TCCGGA 1 cut(s) 345
BspFNI CGCG 2 cut(s) 365, 735
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 577
BspPI GGATC 2 cut(s) 58, 581
BsrFI RCCGGY 2 cut(s) 37, 793
BsrGI TGTACA 1 cut(s) 811
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 2 cut(s) 37, 793
BssECI CCNNGG 2 cut(s) 244, 597
BssMI GATC 4 cut(s) 63, 114, 149, 586
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 3 cut(s) 158, 332, 754
BstAUI TGTACA 1 cut(s) 811
BstBAI YACGTR 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 5 cut(s) 492, 503, 568, 580, 694
BstF5I GGATG 2 cut(s) 531, 785
BstFNI CGCG 2 cut(s) 365, 735
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 4 cut(s) 66, 117, 152, 589
BstMBI GATC 4 cut(s) 63, 114, 149, 586
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 2 cut(s) 560, 569
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 24, 596
BstSFI CTRYAG 2 cut(s) 573, 753
BstUI CGCG 2 cut(s) 365, 735
BstV1I GCAGC 4 cut(s) 135, 559, 587, 862
BstV2I GAAGAC 1 cut(s) 956
BstX2I RGATCY 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 580
BsuI GTATCC 2 cut(s) 353, 596
BsuRI GGCC 3 cut(s) 24, 243, 738
BtsCI GGATG 2 cut(s) 531, 785
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 2 cut(s) 481, 750
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 1 cut(s) 367
Cfr10I RCCGGY 2 cut(s) 37, 793
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 5 cut(s) 222, 274, 433, 812, 896
CspAI ACCGGT 1 cut(s) 793
CspCI CAANNNNNGTGG 2 cut(s) 525, 560
CviAII CATG 7 cut(s) 74, 219, 277, 351, 459, 917, 960
CviQI GTAC 5 cut(s) 222, 274, 433, 812, 896
DdeI CTNAG 5 cut(s) 492, 503, 568, 580, 694
DpnI GATC 4 cut(s) 65, 116, 151, 588
DpnII GATC 4 cut(s) 63, 114, 149, 586
DraI TTTAAA 1 cut(s) 181
DrdI GACNNNNNNGTC 2 cut(s) 463, 921
DseDI GACNNNNNNGTC 2 cut(s) 463, 921
EaeI YGGCCR 2 cut(s) 22, 736
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 28
Eco81I CCTNAGG 1 cut(s) 580
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 7 cut(s) 77, 222, 280, 354, 462, 920, 963
FaqI GGGAC 1 cut(s) 14
FatI CATG 7 cut(s) 73, 218, 276, 350, 458, 916, 959
Fnu4HI GCNGC 6 cut(s) 124, 363, 573, 576, 736, 876
FokI GGATG 2 cut(s) 538, 792
Fsp4HI GCNGC 6 cut(s) 124, 363, 573, 576, 736, 876
FspBI CTAG 3 cut(s) 132, 245, 1042
GlaI GCGC 1 cut(s) 366
GluI GCNGC 6 cut(s) 124, 363, 573, 576, 736, 876
HaeIII GGCC 3 cut(s) 24, 243, 738
HapII CCGG 5 cut(s) 21, 38, 346, 597, 794
HhaI GCGC 1 cut(s) 367
Hin1II CATG 7 cut(s) 77, 222, 280, 354, 462, 920, 963
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 3 cut(s) 166, 338, 1002
HindII GTYRAC 3 cut(s) 166, 338, 1002
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 5 cut(s) 9, 17, 128, 307, 626
HpaI GTTAAC 2 cut(s) 166, 1002
HpaII CCGG 5 cut(s) 21, 38, 346, 597, 794
HphI GGTGA 4 cut(s) 124, 596, 758, 814
Hpy166II GTNNAC 4 cut(s) 166, 338, 814, 1002
Hpy188I TCNGA 3 cut(s) 16, 532, 697
Hpy188III TCNNGA 9 cut(s) 6, 132, 346, 454, 711, 777, 863, 890, 926
Hpy8I GTNNAC 4 cut(s) 166, 338, 814, 1002
Hpy99I CGWCG 1 cut(s) 852
HpyAV CCTTC 2 cut(s) 45, 636
HpyCH4III ACNGT 3 cut(s) 158, 332, 754
HpyCH4IV ACGT 4 cut(s) 340, 485, 610, 953
HpyCH4V TGCA 7 cut(s) 215, 239, 323, 542, 575, 878, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 560, 569
HpyF3I CTNAG 5 cut(s) 492, 503, 568, 580, 694
HpySE526I ACGT 4 cut(s) 340, 485, 610, 953
Hsp92II CATG 7 cut(s) 77, 222, 280, 354, 462, 920, 963
HspAI GCGC 1 cut(s) 365
Kpn2I TCCGGA 1 cut(s) 345
KspAI GTTAAC 2 cut(s) 166, 1002
Kzo9I GATC 4 cut(s) 63, 114, 149, 586
Lsp1109I GCAGC 4 cut(s) 135, 559, 587, 862
LweI GCATC 1 cut(s) 862
MaeI CTAG 3 cut(s) 132, 245, 1042
MaeII ACGT 4 cut(s) 340, 485, 610, 953
MaeIII GTNAC 3 cut(s) 680, 746, 757
MalI GATC 4 cut(s) 65, 116, 151, 588
MboI GATC 4 cut(s) 63, 114, 149, 586
MboII GAAGA 4 cut(s) 269, 295, 953, 956
MfeI CAATTG 1 cut(s) 1013
MflI RGATCY 1 cut(s) 63
MluCI AATT 8 cut(s) 183, 318, 384, 933, 945, 969, 1013, 1021
MlyI GAGTC 3 cut(s) 122, 301, 620
MmeI TCCRAC 1 cut(s) 802
MnlI CCTC 4 cut(s) 57, 459, 793, 827
MroI TCCGGA 1 cut(s) 345
MseI TTAA 7 cut(s) 165, 180, 312, 638, 948, 975, 1001
MslI CAYNNNNRTG 3 cut(s) 372, 755, 906
MspI CCGG 5 cut(s) 21, 38, 346, 597, 794
MspR9I CCNGG 2 cut(s) 26, 598
MunI CAATTG 1 cut(s) 1013
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 2 cut(s) 365, 735
MwoI GCNNNNNNNGC 2 cut(s) 560, 569
NciI CCSGG 1 cut(s) 598
NdeII GATC 4 cut(s) 63, 114, 149, 586
NlaIII CATG 7 cut(s) 77, 222, 280, 354, 462, 920, 963
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 746, 757
NspI RCATGY 1 cut(s) 222
OliI CACNNNNGTG 2 cut(s) 755, 906
PceI AGGCCT 1 cut(s) 243
PciI ACATGT 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 1042
PfeI GAWTC 2 cut(s) 9, 17
PflMI CCANNNNNTGG 1 cut(s) 74
PinAI ACCGGT 1 cut(s) 793
PkrI GCNGC 6 cut(s) 125, 364, 574, 577, 737, 877
PleI GAGTC 3 cut(s) 122, 301, 620
PpsI GAGTC 3 cut(s) 122, 301, 620
Ppu21I YACGTR 1 cut(s) 611
PpuMI RGGWCCY 1 cut(s) 28
PscI ACATGT 1 cut(s) 218
PshBI ATTAAT 1 cut(s) 948
PsiI TTATAA 2 cut(s) 95, 372
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 28
PspPPI RGGWCCY 1 cut(s) 28
PstI CTGCAG 1 cut(s) 577
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 5 cut(s) 223, 275, 434, 813, 897
RsaNI GTAC 5 cut(s) 222, 274, 433, 812, 896
RseI CAYNNNNRTG 3 cut(s) 372, 755, 906
SaqAI TTAA 7 cut(s) 165, 180, 312, 638, 948, 975, 1001
SatI GCNGC 6 cut(s) 124, 363, 573, 576, 736, 876
Sau3AI GATC 4 cut(s) 63, 114, 149, 586
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 3 cut(s) 122, 301, 620
ScrFI CCNGG 2 cut(s) 26, 598
SfaNI GCATC 1 cut(s) 862
SfcI CTRYAG 2 cut(s) 573, 753
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 3 cut(s) 372, 755, 906
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 8 cut(s) 183, 318, 384, 933, 945, 969, 1013, 1021
SseBI AGGCCT 1 cut(s) 243
SsiI CCGC 2 cut(s) 363, 735
SspMI CTAG 3 cut(s) 132, 245, 1042
StuI AGGCCT 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 24, 596
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 3 cut(s) 158, 332, 754
TaiI ACGT 4 cut(s) 343, 488, 613, 956
TaqI TCGA 2 cut(s) 49, 152
TasI AATT 8 cut(s) 183, 318, 384, 933, 945, 969, 1013, 1021
TatI WGTACW 4 cut(s) 273, 432, 811, 895
TauI GCSGC 2 cut(s) 365, 738
TfiI GAWTC 2 cut(s) 9, 17
Tru1I TTAA 7 cut(s) 165, 180, 312, 638, 948, 975, 1001
Tru9I TTAA 7 cut(s) 165, 180, 312, 638, 948, 975, 1001
TscAI CASTG 2 cut(s) 481, 757
TseFI GTSAC 2 cut(s) 746, 757
TseI GCWGC 4 cut(s) 123, 572, 575, 875
Tsp45I GTSAC 2 cut(s) 746, 757
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 2 cut(s) 481, 757
Van91I CCANNNNNTGG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 1 cut(s) 948
XbaI TCTAGA 1 cut(s) 131
XceI RCATGY 1 cut(s) 222
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XmaJI CCTAGG 1 cut(s) 244
XspI CTAG 3 cut(s) 132, 245, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.