Rmu_sc0013155.1_g000007

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013155.1
OG0015757
Physical Location & Seq
Forward (+)
30040 .. 31199
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013155.1_g000007.1.cds

Sequence Viewer

Length: 621 bp
atgtcccgaatctgggaatccggccaggtccccaaaccggcgaagcctcgaaggacagacgagatcccaactcatggcactaccatgatttttataaagacgaatgaactggtaatcgttgggagcgactctagaattagcaacggcagatcgaaccgtatagttaacgatgatgtgtttaaaattgaagcaataacttcaaatatgtatgcaacatgtacgggattgtttcgttcaggcctaggattgtttgaagatttgcgaaagcagtacatggagaagaaaaactctggtgttgaaccgactcttaaagaaattgcagattacggtcggcaacgtatccggacatgggacaatgacgcgcattatagtgttattggaattgaaaatgggaagccccaagcttatggttttactgataaagaaaagtacaaggatactgtggctctcaagacatggtggtcatttggtagtggtggaaagtatgctaagccaaaacttagagctgattacaatagggggatgtctgaaccacttgcattgaagttagctgaaacgacttttttagctgcagccttaggtgatccatttaccgggggatacctacgtggtaatttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

206

Amino Acids

23.15

Weight (kDa)

9.4

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 95
AasI GACNNNNNNGTC 1 cut(s) 460
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 1 cut(s) 362
AccIII TCCGGA 1 cut(s) 342
AclWI GGATC 2 cut(s) 58, 578
AcoI YGGCCR 1 cut(s) 22
AfaI GTAC 3 cut(s) 220, 272, 431
AfiI CCNNNNNNNGG 6 cut(s) 12, 13, 37, 74, 349, 593
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 6 cut(s) 188, 201, 254, 299, 386, 544
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 4 cut(s) 404, 506, 551, 569
AluI AGCT 4 cut(s) 404, 506, 551, 569
AlwI GGATC 2 cut(s) 58, 578
Aor13HI TCCGGA 1 cut(s) 342
AoxI GGCC 2 cut(s) 22, 238
ApeKI GCWGC 2 cut(s) 569, 572
AspA2I CCTAGG 1 cut(s) 241
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 1 cut(s) 593
AvaII GGWCC 1 cut(s) 28
AvrII CCTAGG 1 cut(s) 241
AxyI CCTNAGG 1 cut(s) 577
BaeI ACNNNNGTAYC 1 cut(s) 592
BbvI GCAGC 2 cut(s) 556, 584
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 26
BciVI GTATCC 3 cut(s) 350, 430, 593
BcnI CCSGG 1 cut(s) 595
BfaI CTAG 2 cut(s) 132, 242
BfmI CTRYAG 1 cut(s) 570
BfuI GTATCC 3 cut(s) 350, 430, 593
BisI GCNGC 2 cut(s) 570, 573
BlnI CCTAGG 1 cut(s) 241
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 2 cut(s) 571, 574
Bme1390I CCNGG 2 cut(s) 26, 595
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 26, 595
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 608
BsaJI CCNNGG 2 cut(s) 241, 594
BsaWI WCCGGW 1 cut(s) 342
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 13, 37, 74, 349, 593
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 114
Bse21I CCTNAGG 1 cut(s) 577
BseAI TCCGGA 1 cut(s) 342
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 241, 594
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 6 cut(s) 12, 13, 37, 74, 349, 593
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 556, 584
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 24, 240
BsiEI CGRYCG 1 cut(s) 331
BsiSI CCGG 4 cut(s) 21, 38, 343, 594
BslFI GGGAC 2 cut(s) 14, 365
BslI CCNNNNNNNGG 6 cut(s) 12, 13, 37, 74, 349, 593
BsmFI GGGAC 2 cut(s) 14, 365
BsnI GGCC 2 cut(s) 24, 240
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 3 cut(s) 63, 149, 583
Bsp1720I GCTNAGC 1 cut(s) 489
BspANI GGCC 2 cut(s) 24, 240
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 2 cut(s) 58, 578
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 241, 594
BssMI GATC 3 cut(s) 63, 149, 583
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 3 cut(s) 158, 329, 442
BstBAI YACGTR 1 cut(s) 608
BstDEI CTNAG 3 cut(s) 489, 500, 577
BstF5I GGATG 1 cut(s) 528
BstFNI CGCG 1 cut(s) 362
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 3 cut(s) 66, 152, 586
BstMBI GATC 3 cut(s) 63, 149, 583
BstMCI CGRYCG 1 cut(s) 331
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 2 cut(s) 24, 593
BstSFI CTRYAG 1 cut(s) 570
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 556, 584
BstX2I RGATCY 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 407
BstYI RGATCY 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 577
BsuI GTATCC 3 cut(s) 350, 430, 593
BsuRI GGCC 2 cut(s) 24, 240
BtsCI GGATG 1 cut(s) 528
CfoI GCGC 1 cut(s) 364
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 368
Csp6I GTAC 3 cut(s) 219, 271, 430
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviAII CATG 6 cut(s) 74, 85, 216, 274, 348, 456
CviQI GTAC 3 cut(s) 219, 271, 430
DdeI CTNAG 3 cut(s) 489, 500, 577
DpnI GATC 3 cut(s) 65, 151, 585
DpnII GATC 3 cut(s) 63, 149, 583
DraI TTTAAA 1 cut(s) 181
DrdI GACNNNNNNGTC 1 cut(s) 460
DseDI GACNNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 28
Eco81I CCTNAGG 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 6 cut(s) 77, 88, 219, 277, 351, 459
FaqI GGGAC 2 cut(s) 14, 365
FatI CATG 6 cut(s) 73, 84, 215, 273, 347, 455
Fnu4HI GCNGC 2 cut(s) 570, 573
FokI GGATG 1 cut(s) 535
Fsp4HI GCNGC 2 cut(s) 570, 573
FspBI CTAG 2 cut(s) 132, 242
GlaI GCGC 1 cut(s) 363
GluI GCNGC 2 cut(s) 570, 573
HaeIII GGCC 2 cut(s) 24, 240
HapII CCGG 4 cut(s) 21, 38, 343, 594
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 364
Hin1II CATG 6 cut(s) 77, 88, 219, 277, 351, 459
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 4 cut(s) 9, 17, 128, 304
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 4 cut(s) 21, 38, 343, 594
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 1 cut(s) 529
Hpy188III TCNNGA 4 cut(s) 6, 132, 343, 451
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 1 cut(s) 45
HpyCH4III ACNGT 3 cut(s) 158, 329, 442
HpyCH4IV ACGT 2 cut(s) 337, 607
HpyCH4V TGCA 4 cut(s) 212, 320, 539, 572
HpyF3I CTNAG 3 cut(s) 489, 500, 577
HpySE526I ACGT 2 cut(s) 337, 607
Hsp92II CATG 6 cut(s) 77, 88, 219, 277, 351, 459
HspAI GCGC 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 342
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 3 cut(s) 63, 149, 583
LmnI GCTCC 1 cut(s) 123
LpnPI CCDG 9 cut(s) 11, 34, 38, 51, 95, 222, 276, 356, 607
Lsp1109I GCAGC 2 cut(s) 556, 584
MaeI CTAG 2 cut(s) 132, 242
MaeII ACGT 2 cut(s) 337, 607
MalI GATC 3 cut(s) 65, 151, 585
MboI GATC 3 cut(s) 63, 149, 583
MboII GAAGA 2 cut(s) 266, 292
MflI RGATCY 1 cut(s) 63
MluCI AATT 5 cut(s) 135, 183, 315, 381, 613
MlyI GAGTC 2 cut(s) 122, 298
MnlI CCTC 1 cut(s) 57
MroI TCCGGA 1 cut(s) 342
MseI TTAA 3 cut(s) 165, 180, 309
MslI CAYNNNNRTG 2 cut(s) 83, 369
MspI CCGG 4 cut(s) 21, 38, 343, 594
MspR9I CCNGG 2 cut(s) 26, 595
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 1 cut(s) 362
NciI CCSGG 1 cut(s) 595
NdeII GATC 3 cut(s) 63, 149, 583
NlaIII CATG 6 cut(s) 77, 88, 219, 277, 351, 459
NlaIV GGNNCC 1 cut(s) 30
NspI RCATGY 1 cut(s) 219
PceI AGGCCT 1 cut(s) 240
PciI ACATGT 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 2 cut(s) 9, 17
PflMI CCANNNNNTGG 1 cut(s) 74
PkrI GCNGC 2 cut(s) 571, 574
PleI GAGTC 2 cut(s) 122, 298
PpsI GAGTC 2 cut(s) 122, 298
Ppu21I YACGTR 1 cut(s) 608
PpuMI RGGWCCY 1 cut(s) 28
PscI ACATGT 1 cut(s) 215
PsiI TTATAA 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 28
PspPPI RGGWCCY 1 cut(s) 28
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 3 cut(s) 220, 272, 431
RsaNI GTAC 3 cut(s) 219, 271, 430
RseI CAYNNNNRTG 2 cut(s) 83, 369
SaqAI TTAA 3 cut(s) 165, 180, 309
SatI GCNGC 2 cut(s) 570, 573
Sau3AI GATC 3 cut(s) 63, 149, 583
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 2 cut(s) 122, 298
ScrFI CCNGG 2 cut(s) 26, 595
SetI ASST 9 cut(s) 30, 340, 406, 508, 553, 571, 583, 606, 610
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 2 cut(s) 83, 369
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 5 cut(s) 135, 183, 315, 381, 613
SseBI AGGCCT 1 cut(s) 240
SspMI CTAG 2 cut(s) 132, 242
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 24, 593
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 3 cut(s) 158, 329, 442
TaiI ACGT 2 cut(s) 340, 610
TaqI TCGA 2 cut(s) 49, 152
TasI AATT 5 cut(s) 135, 183, 315, 381, 613
TatI WGTACW 2 cut(s) 270, 429
TfiI GAWTC 2 cut(s) 9, 17
Tru1I TTAA 3 cut(s) 165, 180, 309
Tru9I TTAA 3 cut(s) 165, 180, 309
TseI GCWGC 2 cut(s) 569, 572
TspDTI ATGAA 1 cut(s) 120
Van91I CCANNNNNTGG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 28
XbaI TCTAGA 1 cut(s) 131
XceI RCATGY 1 cut(s) 219
XmaJI CCTAGG 1 cut(s) 241
XspI CTAG 2 cut(s) 132, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.