Rroxscaffold_1G00073730

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015757
Physical Location & Seq
Reverse (-)
94475008 .. 94479359
4352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073730.1

Sequence Viewer

Length: 1584 bp
ATGACTGACGTCAAGAGCCAAAACCCTAATTTGGACCGTCCGGCGAATTGCCGAGTGGAAGAACTCGATGAGGACGATGTGGGCAAAAGCCCTAAACAGACGAAGCGCCGAAGGGAAGAATTCTACGAGAGCGCTGCTGGCACTGGGAATAGTACATTGTGGAACAAGGAGCCTCCAATGGTAGATGATGGATCTGCTAATGGCTCCTCTTTGGTGCTGCCGCAAAAGGAAGATACGGGTAGCGGTGGCAACGAAGATAGTGATGTAGAAGAAGTTAGCGCTACAGAAGAAGATTCTGATTCAGCAGACTTTATACTAGATTATGGTGGTGGCGTAGATGAGGGTGACTATTTTGGATATTGTGAATTATGTGTAGACAATGCAGTCTGCAAAGGTTGTGGTAACAAGGATAGCAGCCCTGTACGTGAGGAAGAACGTTTGAATCCATTGGAATGTAGAATTTGTGGTACCAGTGAGCATAGGACTGAGTACTGTGCATCTCGGCTGATGAACAAGTGGGGGAAGGAGCAGGAAGAAAGGGAGAAGATGATGCTTTCAAGGAGTGGGGAATCCGGCCAGGTCCCCAAACCGGTGAAGCCTCGAAGGAAAGACGAGATCCCCACTCATGGCACTACCATCATTTTTATAAAGACGAATGAACTGGTGATCGTTGGCAGCGACTCTAGATGTAGCAACCGCAGATCGAACCGTATAGTTAACGATGATGGGTTTAAAATTGAAGCAATAACTTCAAATATTTATGCAACATGTACGGGATTGTTTAGTGCAGCCCAAGTATTGTTTGAAGATTTGCGAAAGCAGTACATGGAGAAGAAAAACTCTGGTGTTGAACCGACTCTTAAAGAAATTGCAGATTACGGTCGGCAACATATCTGGACATGGGACAATGGCGTGCATTATAATGTTATTGGAACTGAAAATGGGAAGCCCCAAGCTTATGGTTTTGTTGATAAAGAAAAGTACAAGGATAGTGTGGCTCTCAAGACATGGTGGTCATTTGGCAGTGGTACAAAGTATGCTAAGCCAGAGCTCAATGCTAATTACAACAGGGGGATGTCTGAACCACTTGCATTGAATTTAGCTGAAACGGCTTTTTTAGCTGCAGCTTTAGGTGATCCATTTACCGGGGGATACCTACGTGTATTTGTCTTTGACTCAAACGGATTAAGAAAGGAGTATTCCAAGACTACATTGGAGTTGTATTTGAGTTACTACTCACTCTCAGATGAGTTCCATTCAAGAACAATCTACATCTTTTCTCGCGGCCAGATTGGTGACACTGTAGTGACCCAATGGTTTAGTTCTGGATGTCTTATTGAACCGGTTGATGTGATGGTCGTACATCCTGTTGGATATATTGGGGGATATATTGTTCGTCGTGTGGTTTTCCAAGAGAAAGATGCTGCAACCGTTATTCAAGAAGTACAACGCCAATGTGAAAGATATGGTTGTCATGATAGAACTGATGAGGAAATTAATACGTTTCCGTGTCTTCAAATTGTTAATGAGCGATTTCATTTTGCAAAGTTAACAAAAGAGCAATTGAGCAATTTACTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

527

Amino Acids

59.27

Weight (kDa)

5.02

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 209 - 389 8.6e-16 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 647, 921
AasI GACNNNNNNGTC 2 cut(s) 383, 1012
AatII GACGTC 1 cut(s) 12
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccI GTMKAC 1 cut(s) 375
AccII CGCG 1 cut(s) 1284
AciI CCGC 4 cut(s) 221, 243, 697, 1284
AclI AACGTT 1 cut(s) 436
AclWI GGATC 3 cut(s) 199, 610, 1130
AcoI YGGCCR 2 cut(s) 574, 1285
AcsI RAATTY 3 cut(s) 119, 459, 1096
AcyI GRCGYC 1 cut(s) 9
AfeI AGCGCT 2 cut(s) 133, 280
AfiI CCNNNNNNNGG 4 cut(s) 31, 589, 626, 1145
AflIII ACRYGT 2 cut(s) 767, 1159
AgeI ACCGGT 2 cut(s) 589, 1342
AjnI CCWGG 1 cut(s) 576
AleI CACNNNNGTG 1 cut(s) 1304
AloI GAACNNNNNNTCC 2 cut(s) 1377, 1409
AluBI AGCT 5 cut(s) 956, 1051, 1103, 1121, 1127
AluI AGCT 5 cut(s) 956, 1051, 1103, 1121, 1127
Alw21I GWGCWC 1 cut(s) 1053
AlwI GGATC 3 cut(s) 199, 610, 1130
Aor51HI AGCGCT 2 cut(s) 133, 280
AoxI GGCC 2 cut(s) 574, 1285
ApeKI GCWGC 8 cut(s) 134, 217, 414, 675, 788, 1121, 1124, 1424
ApoI RAATTY 3 cut(s) 119, 459, 1096
AseI ATTAAT 1 cut(s) 1497
AsiGI ACCGGT 2 cut(s) 589, 1342
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 3 cut(s) 108, 134, 281
AspS9I GGNCC 2 cut(s) 34, 580
AsuC2I CCSGG 1 cut(s) 1147
AsuHPI GGTGA 5 cut(s) 356, 604, 676, 1145, 1307
AvaII GGWCC 2 cut(s) 34, 580
BaeI ACNNNNGTAYC 2 cut(s) 1144, 1177
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 1053
BbsI GAAGAC 1 cut(s) 1505
Bbv12I GWGCWC 1 cut(s) 1053
BbvI GCAGC 8 cut(s) 121, 204, 426, 687, 800, 1108, 1136, 1411
BccI CCATC 4 cut(s) 182, 644, 719, 1348
BceAI ACGGC 1 cut(s) 1125
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 1 cut(s) 578
BciVI GTATCC 1 cut(s) 1145
BcnI CCSGG 1 cut(s) 1147
BfaI CTAG 2 cut(s) 317, 684
BfmI CTRYAG 3 cut(s) 282, 1122, 1302
BfoI RGCGCY 3 cut(s) 109, 135, 282
BfuI GTATCC 1 cut(s) 1145
BlpI GCTNAGC 1 cut(s) 1041
BmcAI AGTACT 1 cut(s) 491
Bme1390I CCNGG 2 cut(s) 578, 1147
Bme18I GGWCC 2 cut(s) 34, 580
BmgT120I GGNCC 2 cut(s) 34, 580
BmiI GGNNCC 4 cut(s) 171, 205, 469, 582
BmrFI CCNGG 2 cut(s) 578, 1147
BmrI ACTGGG 1 cut(s) 153
BmsI GCATC 3 cut(s) 506, 540, 1411
BmuI ACTGGG 1 cut(s) 153
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 1505
Bpu1102I GCTNAGC 1 cut(s) 1041
BpuEI CTTGAG 1 cut(s) 986
BpuMI CCSGG 1 cut(s) 1147
BsaAI YACGTR 2 cut(s) 425, 1160
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 1146
BsaWI WCCGGW 2 cut(s) 589, 1342
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 589, 626, 1145
Bse118I RCCGGY 2 cut(s) 589, 1342
Bse1I ACTGG 3 cut(s) 148, 471, 666
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 1146
BseGI GGATG 3 cut(s) 1080, 1334, 1363
BseLI CCNNNNNNNGG 4 cut(s) 31, 589, 626, 1145
BseMII CTCAG 2 cut(s) 477, 1257
BseNI ACTGG 3 cut(s) 148, 471, 666
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 8 cut(s) 121, 204, 426, 687, 800, 1108, 1136, 1411
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 1 cut(s) 1284
Bsh1285I CGRYCG 1 cut(s) 883
BshFI GGCC 2 cut(s) 576, 1287
BshNI GGYRCC 1 cut(s) 467
BshTI ACCGGT 2 cut(s) 589, 1342
BsiEI CGRYCG 1 cut(s) 883
BsiHKAI GWGCWC 1 cut(s) 1053
BsiSI CCGG 5 cut(s) 41, 573, 590, 1146, 1343
BslFI GGGAC 2 cut(s) 566, 917
BslI CCNNNNNNNGG 4 cut(s) 31, 589, 626, 1145
BsmFI GGGAC 2 cut(s) 566, 917
BsnI GGCC 2 cut(s) 576, 1287
Bsp1286I GDGCHC 1 cut(s) 1053
Bsp143I GATC 5 cut(s) 191, 615, 666, 701, 1135
Bsp1720I GCTNAGC 1 cut(s) 1041
BspACI CCGC 4 cut(s) 221, 243, 697, 1284
BspANI GGCC 2 cut(s) 576, 1287
BspCNI CTCAG 2 cut(s) 478, 1256
BspFNI CGCG 1 cut(s) 1284
BspHI TCATGA 1 cut(s) 1474
BspLI GGNNCC 4 cut(s) 171, 205, 469, 582
BspMAI CTGCAG 1 cut(s) 1126
BspPI GGATC 3 cut(s) 199, 610, 1130
BspT107I GGYRCC 1 cut(s) 467
BsrFI RCCGGY 2 cut(s) 589, 1342
BsrI ACTGG 3 cut(s) 148, 471, 666
BssAI RCCGGY 2 cut(s) 589, 1342
BssECI CCNNGG 1 cut(s) 1146
BssMI GATC 5 cut(s) 191, 615, 666, 701, 1135
BssNI GRCGYC 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 6 cut(s) 38, 494, 710, 881, 1303, 1432
BstACI GRCGYC 1 cut(s) 9
BstBAI YACGTR 2 cut(s) 425, 1160
BstC8I GCNNGC 2 cut(s) 139, 914
BstDEI CTNAG 3 cut(s) 486, 1041, 1243
BstF5I GGATG 3 cut(s) 1080, 1334, 1363
BstFNI CGCG 1 cut(s) 1284
BstH2I RGCGCY 3 cut(s) 109, 135, 282
BstHHI GCGC 3 cut(s) 108, 134, 281
BstKTI GATC 5 cut(s) 194, 618, 669, 704, 1138
BstMBI GATC 5 cut(s) 191, 615, 666, 701, 1135
BstMCI CGRYCG 1 cut(s) 883
BstMWI GCNNNNNNNGC 3 cut(s) 138, 1109, 1118
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 1 cut(s) 771
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 576, 1145
BstSFI CTRYAG 3 cut(s) 282, 1122, 1302
BstUI CGCG 1 cut(s) 1284
BstV1I GCAGC 8 cut(s) 121, 204, 426, 687, 800, 1108, 1136, 1411
BstV2I GAAGAC 1 cut(s) 1505
BstX2I RGATCY 2 cut(s) 191, 615
BstXI CCANNNNNNTGG 1 cut(s) 959
BstYI RGATCY 2 cut(s) 191, 615
BsuI GTATCC 1 cut(s) 1145
BsuRI GGCC 2 cut(s) 576, 1287
BtsCI GGATG 3 cut(s) 1080, 1334, 1363
BtsI GCAGTG 1 cut(s) 1030
BtsIMutI CAGTG 4 cut(s) 141, 478, 1030, 1299
Cac8I GCNNGC 2 cut(s) 139, 914
CciI TCATGA 1 cut(s) 1474
CfoI GCGC 3 cut(s) 108, 134, 281
Cfr10I RCCGGY 2 cut(s) 589, 1342
Cfr13I GGNCC 2 cut(s) 34, 580
CspAI ACCGGT 2 cut(s) 589, 1342
CspCI CAANNNNNGTGG 4 cut(s) 379, 414, 1074, 1109
CviAII CATG 6 cut(s) 626, 768, 826, 900, 1008, 1475
DdeI CTNAG 3 cut(s) 486, 1041, 1243
DpnI GATC 5 cut(s) 193, 617, 668, 703, 1137
DpnII GATC 5 cut(s) 191, 615, 666, 701, 1135
DraI TTTAAA 1 cut(s) 733
DrdI GACNNNNNNGTC 2 cut(s) 383, 1012
DseDI GACNNNNNNGTC 2 cut(s) 383, 1012
EaeI YGGCCR 2 cut(s) 574, 1285
Ecl136II GAGCTC 1 cut(s) 1051
Eco24I GRGCYC 1 cut(s) 1053
Eco47I GGWCC 2 cut(s) 34, 580
Eco47III AGCGCT 2 cut(s) 133, 280
Eco53kI GAGCTC 1 cut(s) 1051
EcoICRI GAGCTC 1 cut(s) 1051
EcoO109I RGGNCCY 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 576
EcoT38I GRGCYC 1 cut(s) 1053
FaeI CATG 6 cut(s) 629, 771, 829, 903, 1011, 1478
FaqI GGGAC 2 cut(s) 566, 917
FatI CATG 6 cut(s) 625, 767, 825, 899, 1007, 1474
FblI GTMKAC 1 cut(s) 375
FokI GGATG 3 cut(s) 1087, 1341, 1350
FriOI GRGCYC 1 cut(s) 1053
FspBI CTAG 2 cut(s) 317, 684
GlaI GCGC 3 cut(s) 107, 133, 280
HaeII RGCGCY 3 cut(s) 109, 135, 282
HaeIII GGCC 2 cut(s) 576, 1287
HapII CCGG 5 cut(s) 41, 573, 590, 1146, 1343
HhaI GCGC 3 cut(s) 108, 134, 281
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 6 cut(s) 629, 771, 829, 903, 1011, 1478
Hin6I GCGC 3 cut(s) 106, 132, 279
HinP1I GCGC 3 cut(s) 106, 132, 279
HincII GTYRAC 2 cut(s) 718, 1551
HindII GTYRAC 2 cut(s) 718, 1551
HindIII AAGCTT 1 cut(s) 954
HinfI GANTC 7 cut(s) 293, 299, 442, 569, 680, 856, 1175
HpaI GTTAAC 2 cut(s) 718, 1551
HpaII CCGG 5 cut(s) 41, 573, 590, 1146, 1343
HphI GGTGA 5 cut(s) 356, 604, 676, 1145, 1307
Hpy166II GTNNAC 3 cut(s) 376, 718, 1551
Hpy188I TCNGA 3 cut(s) 298, 1081, 1246
Hpy188III TCNNGA 8 cut(s) 13, 684, 895, 1003, 1260, 1326, 1439, 1475
Hpy8I GTNNAC 3 cut(s) 376, 718, 1551
Hpy99I CGWCG 1 cut(s) 1401
HpyAV CCTTC 3 cut(s) 105, 517, 597
HpyCH4III ACNGT 6 cut(s) 38, 494, 710, 881, 1303, 1432
HpyCH4IV ACGT 5 cut(s) 9, 424, 436, 1159, 1502
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 1109, 1118
HpyF3I CTNAG 3 cut(s) 486, 1041, 1243
HpySE526I ACGT 5 cut(s) 9, 424, 436, 1159, 1502
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 6 cut(s) 629, 771, 829, 903, 1011, 1478
HspAI GCGC 3 cut(s) 106, 132, 279
KpnI GGTACC 1 cut(s) 471
KspAI GTTAAC 2 cut(s) 718, 1551
Kzo9I GATC 5 cut(s) 191, 615, 666, 701, 1135
LmnI GCTCC 3 cut(s) 169, 209, 526
Lsp1109I GCAGC 8 cut(s) 121, 204, 426, 687, 800, 1108, 1136, 1411
LweI GCATC 3 cut(s) 506, 540, 1411
MaeI CTAG 2 cut(s) 317, 684
MaeII ACGT 5 cut(s) 9, 424, 436, 1159, 1502
MaeIII GTNAC 5 cut(s) 344, 401, 1229, 1295, 1306
MalI GATC 5 cut(s) 193, 617, 668, 703, 1137
MboI GATC 5 cut(s) 191, 615, 666, 701, 1135
MfeI CAATTG 1 cut(s) 1562
MflI RGATCY 2 cut(s) 191, 615
MhlI GDGCHC 1 cut(s) 1053
MlyI GAGTC 3 cut(s) 674, 850, 1169
MmeI TCCRAC 1 cut(s) 1351
MnlI CCTC 7 cut(s) 64, 183, 217, 334, 421, 609, 1483
MseI TTAA 7 cut(s) 717, 732, 861, 1187, 1497, 1524, 1550
MslI CAYNNNNRTG 3 cut(s) 451, 921, 1304
MspI CCGG 5 cut(s) 41, 573, 590, 1146, 1343
MspR9I CCNGG 2 cut(s) 578, 1147
MunI CAATTG 1 cut(s) 1562
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 1 cut(s) 1284
MwoI GCNNNNNNNGC 3 cut(s) 138, 1109, 1118
NciI CCSGG 1 cut(s) 1147
NdeII GATC 5 cut(s) 191, 615, 666, 701, 1135
NlaIII CATG 6 cut(s) 629, 771, 829, 903, 1011, 1478
NlaIV GGNNCC 4 cut(s) 171, 205, 469, 582
NmeAIII GCCGAG 2 cut(s) 77, 481
NmuCI GTSAC 3 cut(s) 344, 1295, 1306
NspI RCATGY 1 cut(s) 771
OliI CACNNNNGTG 1 cut(s) 1304
PagI TCATGA 1 cut(s) 1474
PciI ACATGT 1 cut(s) 767
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 675
PfeI GAWTC 4 cut(s) 293, 299, 442, 569
PinAI ACCGGT 2 cut(s) 589, 1342
PleI GAGTC 3 cut(s) 674, 850, 1169
PpsI GAGTC 3 cut(s) 674, 850, 1169
Ppu21I YACGTR 2 cut(s) 425, 1160
PpuMI RGGWCCY 1 cut(s) 580
PscI ACATGT 1 cut(s) 767
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 1497
PsiI TTATAA 2 cut(s) 647, 921
Psp124BI GAGCTC 1 cut(s) 1053
Psp1406I AACGTT 1 cut(s) 436
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 4 cut(s) 171, 205, 469, 582
PspPI GGNCC 2 cut(s) 34, 580
PspPPI RGGWCCY 1 cut(s) 580
PstI CTGCAG 1 cut(s) 1126
PsuI RGATCY 2 cut(s) 191, 615
RseI CAYNNNNRTG 3 cut(s) 451, 921, 1304
SacI GAGCTC 1 cut(s) 1053
SaqAI TTAA 7 cut(s) 717, 732, 861, 1187, 1497, 1524, 1550
Sau3AI GATC 5 cut(s) 191, 615, 666, 701, 1135
Sau96I GGNCC 2 cut(s) 34, 580
ScaI AGTACT 1 cut(s) 491
SchI GAGTC 3 cut(s) 674, 850, 1169
ScrFI CCNGG 2 cut(s) 578, 1147
SduI GDGCHC 1 cut(s) 1053
SfaNI GCATC 3 cut(s) 506, 540, 1411
SfcI CTRYAG 3 cut(s) 282, 1122, 1302
SinI GGWCC 2 cut(s) 34, 580
SmiMI CAYNNNNRTG 3 cut(s) 451, 921, 1304
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
SsiI CCGC 4 cut(s) 221, 243, 697, 1284
SspI AATATT 1 cut(s) 757
SspMI CTAG 2 cut(s) 317, 684
SstI GAGCTC 1 cut(s) 1053
StyD4I CCNGG 2 cut(s) 576, 1145
TaaI ACNGT 6 cut(s) 38, 494, 710, 881, 1303, 1432
TaiI ACGT 5 cut(s) 12, 427, 439, 1162, 1505
TaqI TCGA 3 cut(s) 66, 601, 704
TatI WGTACW 5 cut(s) 152, 489, 822, 981, 1444
TauI GCSGC 2 cut(s) 223, 1287
TfiI GAWTC 4 cut(s) 293, 299, 442, 569
Tru1I TTAA 7 cut(s) 717, 732, 861, 1187, 1497, 1524, 1550
Tru9I TTAA 7 cut(s) 717, 732, 861, 1187, 1497, 1524, 1550
TscAI CASTG 4 cut(s) 148, 478, 1030, 1306
TseFI GTSAC 3 cut(s) 344, 1295, 1306
TseI GCWGC 8 cut(s) 134, 217, 414, 675, 788, 1121, 1124, 1424
Tsp45I GTSAC 3 cut(s) 344, 1295, 1306
TspDTI ATGAA 3 cut(s) 524, 672, 1526
TspGWI ACGGA 2 cut(s) 1197, 1497
TspRI CASTG 4 cut(s) 148, 478, 1030, 1306
VpaK11BI GGWCC 2 cut(s) 34, 580
VspI ATTAAT 1 cut(s) 1497
XapI RAATTY 3 cut(s) 119, 459, 1096
XbaI TCTAGA 1 cut(s) 683
XceI RCATGY 1 cut(s) 771
XcmI CCANNNNNNNNNTGG 1 cut(s) 1210
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 2 cut(s) 317, 684
ZraI GACGTC 1 cut(s) 10
ZrmI AGTACT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.