RchiOBHm_Chr5g0009251

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013762
Physical Location & Seq
Reverse (-)
6180371 .. 6180895
525 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29014

Sequence Viewer

Length: 525 bp
ATGTGTGGAGCCTGCTTAATGATCTTTCCTACAGCTTGGCTTGCAAACCCTTCCAGTTACGTGTTTCAGAGAAAACTTGTGTATGGTAACTCGAGCCCATCACTCGTTAGCATCAAGTTCGTAATCCTCATATCATGTTTCCTCCCGGCCTTGGGATTCTTTGCTTATTCAGCAAAGCAATTCAAGGAAACGCTCTATCTAATAGGCTCAAATGACGAAAGAGCTAATGAGGCCGGGGCAATCAGGGCTTTTATAAGGGGTACTACTTTCTTCTCGCTTGGGATCAGATGTCTACTATGTGCTGGCCCTTCGCTACTATGGTTTTTTGGCCCTATCCCCATGTTTGTGTCCTCTGTTGCTTTAGTCATGATATCCCTCCATGTCGCTGGCAATCGTGCCAGTCCACAATTGCATGTTCCTCCTGAGAATACGGATGAAAGTCACGGAGACAATTCCAATGTTACAAAGGAAGAAGCATGTGGTCACCCTAGTATTGATATAGAGATGCCCCCTCCTGGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

174

Amino Acids

18.86

Weight (kDa)

6.4

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 12 - 127 8.1e-23 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AccI GTMKAC 1 cut(s) 292
AclWI GGATC 1 cut(s) 290
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 3 cut(s) 151, 152, 515
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 1 cut(s) 514
AluBI AGCT 2 cut(s) 35, 224
AluI AGCT 2 cut(s) 35, 224
Alw26I GTCTC 1 cut(s) 441
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 91
AoxI GGCC 4 cut(s) 147, 231, 304, 328
AspS9I GGNCC 2 cut(s) 305, 329
AsuC2I CCSGG 2 cut(s) 146, 235
AsuHPI GGTGA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 91
BanII GRGCYC 1 cut(s) 98
BccI CCATC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 1 cut(s) 516
BcnI CCSGG 2 cut(s) 146, 235
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 1 cut(s) 30
Bme1390I CCNGG 3 cut(s) 146, 235, 516
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 305, 329
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 3 cut(s) 146, 235, 516
BmsI GCATC 2 cut(s) 120, 495
BpuMI CCSGG 2 cut(s) 146, 235
BsaAI YACGTR 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 150, 234
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 152, 515
Bse1I ACTGG 2 cut(s) 54, 399
BseBI CCWGG 1 cut(s) 516
BseDI CCNNGG 2 cut(s) 150, 234
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 151, 152, 515
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 54, 399
BshFI GGCC 4 cut(s) 149, 233, 306, 330
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 2 cut(s) 146, 234
BslI CCNNNNNNNGG 3 cut(s) 151, 152, 515
BsmAI GTCTC 1 cut(s) 441
BsnI GGCC 4 cut(s) 149, 233, 306, 330
BsoBI CYCGRG 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 2 cut(s) 21, 282
BspANI GGCC 4 cut(s) 149, 233, 306, 330
BspCNI CTCAG 1 cut(s) 415
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 10
BspPI GGATC 1 cut(s) 290
BsrI ACTGG 2 cut(s) 54, 399
BssECI CCNNGG 2 cut(s) 150, 234
BssMI GATC 2 cut(s) 21, 282
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 4 cut(s) 13, 42, 304, 388
BstDEI CTNAG 1 cut(s) 423
BstEII GGTNACC 1 cut(s) 482
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 2 cut(s) 24, 285
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 2 cut(s) 21, 282
BstMWI GCNNNNNNNGC 4 cut(s) 41, 170, 230, 245
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 2 cut(s) 416, 480
BstPI GGTNACC 1 cut(s) 482
BstSCI CCNGG 3 cut(s) 144, 233, 514
BstSFI CTRYAG 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 386
BsuRI GGCC 4 cut(s) 149, 233, 306, 330
BtsCI GGATG 1 cut(s) 439
Cac8I GCNNGC 4 cut(s) 13, 42, 304, 388
CciI TCATGA 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 305, 329
Csp6I GTAC 1 cut(s) 261
CviAII CATG 6 cut(s) 135, 340, 367, 380, 413, 477
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 1 cut(s) 423
DpnI GATC 2 cut(s) 23, 284
DpnII GATC 2 cut(s) 21, 282
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 98
Eco32I GATATC 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 91
Eco91I GGTNACC 1 cut(s) 482
EcoO65I GGTNACC 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 514
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 6 cut(s) 138, 343, 370, 383, 416, 480
FatI CATG 6 cut(s) 134, 339, 366, 379, 412, 476
FblI GTMKAC 1 cut(s) 292
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 1 cut(s) 98
FspBI CTAG 1 cut(s) 489
HaeIII GGCC 4 cut(s) 149, 233, 306, 330
HapII CCGG 2 cut(s) 146, 234
Hin1II CATG 6 cut(s) 138, 343, 370, 383, 416, 480
HinfI GANTC 1 cut(s) 156
HpaII CCGG 2 cut(s) 146, 234
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 2 cut(s) 293, 404
Hpy188I TCNGA 2 cut(s) 69, 287
Hpy188III TCNNGA 2 cut(s) 367, 422
Hpy8I GTNNAC 2 cut(s) 293, 404
HpyAV CCTTC 2 cut(s) 60, 318
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 2 cut(s) 44, 412
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 170, 230, 245
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 6 cut(s) 138, 343, 370, 383, 416, 480
Kzo9I GATC 2 cut(s) 21, 282
LmnI GCTCC 1 cut(s) 8
LweI GCATC 2 cut(s) 120, 495
MaeI CTAG 1 cut(s) 489
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 5 cut(s) 56, 86, 440, 460, 482
MalI GATC 2 cut(s) 23, 284
MboI GATC 2 cut(s) 21, 282
MboII GAAGA 2 cut(s) 262, 482
MfeI CAATTG 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 3 cut(s) 179, 407, 451
MnlI CCTC 7 cut(s) 137, 152, 223, 361, 386, 429, 522
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 344
MspI CCGG 2 cut(s) 146, 234
MspR9I CCNGG 3 cut(s) 146, 235, 516
MunI CAATTG 1 cut(s) 407
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 4 cut(s) 41, 170, 230, 245
NciI CCSGG 2 cut(s) 146, 235
NdeII GATC 2 cut(s) 21, 282
NlaIII CATG 6 cut(s) 138, 343, 370, 383, 416, 480
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 2 cut(s) 440, 482
NspI RCATGY 2 cut(s) 416, 480
PaeR7I CTCGAG 1 cut(s) 91
PagI TCATGA 1 cut(s) 366
PfeI GAWTC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 61
PsiI TTATAA 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 514
PspEI GGTNACC 1 cut(s) 482
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 10
PspPI GGNCC 2 cut(s) 305, 329
PspXI VCTCGAGB 1 cut(s) 91
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 17
Sau3AI GATC 2 cut(s) 21, 282
Sau96I GGNCC 2 cut(s) 305, 329
ScrFI CCNGG 3 cut(s) 146, 235, 516
SduI GDGCHC 1 cut(s) 98
SetI ASST 3 cut(s) 37, 63, 226
SfaNI GCATC 2 cut(s) 120, 495
SfcI CTRYAG 1 cut(s) 30
Sfr274I CTCGAG 1 cut(s) 91
SlaI CTCGAG 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
Sse9I AATT 3 cut(s) 179, 407, 451
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 3 cut(s) 144, 233, 514
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 92
TasI AATT 3 cut(s) 179, 407, 451
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 2 cut(s) 440, 482
Tsp45I GTSAC 2 cut(s) 440, 482
TspDTI ATGAA 1 cut(s) 450
TspGWI ACGGA 2 cut(s) 446, 459
XceI RCATGY 2 cut(s) 416, 480
XhoI CTCGAG 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 292
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.