Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0008176.1

Orthogroup ID

OG0013762

Physical Location & Seq

Genomic Coordinates Reverse (-)

22720 .. 24955

Gene Structure 2236 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0008176.1_g000004.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

atgttattgaacaccgggattgggattggatggtttcggcagagaagcttgaggattgaagggtgtggattgatggtaactgttggaattgcagaggtgatcgggattgggatgggagaactaagaagagagatgggtgcccatagtggtgatagcaaagatacaagtcttgctgtgatctcatccagcataacagcagcatatagtttgtgtggagcctgcttaatgatctttcctatagcttggcttgcaaactcctccagtttcatctttcagagtgaacttgtctatggtaacacgagcccattgctcattaccttcaagtttggaagcctcatagcatgttttctcctggccatggcatgctttgctcgatcagcaaagcacttcaaccaagcatactatctaatgagctcccagaacagcaatattgaggccggagatgtggaggcggcaaccagggcagttatagggggaggtcatttcaaatctcttgggattagatgtctacaatgtgctggcattctgctgctatggtttttcggtcctatcccgatgtttgtttcatctgtggctttagtcatattcctccatgacaattatactggtcgacaatatcatgttcgtaatagagtcgattggcaaattcagtcttttcagaaactgaggatttgtgagtctgagattttgtttgcccttcgtgaagataatgtgattggtagagggccatctgggaaagtgtacaaggtcgtgttaagcaatcataaagttattgccgtcaagaagccgtggagagggagagtgaaccagtgtgaaaatgacgatgttgagaaaggtatggtccaagatgatggctctatagcagggtttgacactttggggaggattacgcacaagaacattatgaagctttggtattgttgtactactagagactgccagcagctgttggtttatgattacatgccaaatggtagtcttggtgattgtctgcacaataagaaagcacggttgctgaattggcctacaaggtttaagattgctcttggtgctgctgaggggatttcttatctgcaccatgattgttctccttctattgttcatagaaatgtgaaatcaaacaatatattgttggatggtgatttcggagctcgagtggtggatattggaacaactaatggcgttgatgcatctgtaaaagggccagtcattgcagctgcttgtggttacattgctccagaattttcgcacgcacatgggttgaatgggaagagtgatatatacagctttggtgtggtgatgctggaattggttaccgggaaacttccagttgacccagaactaggagaaaaggacctggtgaagtgggtccgtagcactttggatcagaagggagtggaccatgtccttgaccccaagctcgattcttgttacaaggaagaagtatgcaaggtcctcgatattgggcttctctgcaccgattctcgtccagtcaatcgaccatccatgagaagagtggtggaattgttgcaagaagtgggcagaggagcatccccatattgctaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

521

Amino Acids

57.27

Weight (kDa)

8.32

Isoelectric Point (pI)

39.62

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013762)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr5g0009251 RchiOBHm_Chr5g0035201
rosa_laevigata	RLG00000031650
rosa_multiflora	Rmu_co8136594.1_g000001 Rmu_sc0006233.1_g000004 Rmu_sc0006233.1_g000016 Rmu_sc0008176.1_g000004 Rmu_sc0009464.1_g000001
rosa_roxburghii	Rroxscaffold_1G00045190
rosa_samantha	Rh5AG071600 Rh5AG072400 Rh5BG243000 Rh5CG080600 Rh5CG081300 Rh5DG068200 Rh5DG069000
rosa_wichuraiana	Rw5G006700 Rw5G006760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	137
AccI	GTMKAC	2 cut(s)	508, 610
AciI	CCGC	1 cut(s)	452
AclWI	GGATC	1 cut(s)	1392
AcoI	YGGCCR	1 cut(s)	354
AcsI	RAATTY	2 cut(s)	645, 1241
AfaI	GTAC	2 cut(s)	743, 925
AfiI	CCNNNNNNNGG	4 cut(s)	21, 358, 794, 1343
AgsI	TTSAA	6 cut(s)	10, 59, 322, 391, 487, 1264
AjnI	CCWGG	3 cut(s)	351, 458, 1356
AluBI	AGCT	9 cut(s)	48, 242, 414, 910, 946, 1151, 1217, 1287, 1420
AluI	AGCT	9 cut(s)	48, 242, 414, 910, 946, 1151, 1217, 1287, 1420
Alw21I	GWGCWC	2 cut(s)	416, 1153
Alw26I	GTCTC	1 cut(s)	927
AlwI	GGATC	1 cut(s)	1392
AlwNI	CAGNNNCTG	2 cut(s)	664, 946
Ama87I	CYCGRG	1 cut(s)	1152
AoxI	GGCC	5 cut(s)	354, 435, 725, 1022, 1202
ApeKI	GCWGC	6 cut(s)	197, 529, 943, 1052, 1214, 1217
ApoI	RAATTY	2 cut(s)	645, 1241
AspS9I	GGNCC	8 cut(s)	545, 725, 841, 1202, 1354, 1369, 1399, 1453
AsuC2I	CCSGG	2 cut(s)	16, 1318
AsuHPI	GGTGA	6 cut(s)	109, 161, 995, 1151, 1309, 1372
AvaI	CYCGRG	1 cut(s)	1152
AvaII	GGWCC	6 cut(s)	545, 841, 1354, 1369, 1399, 1453
BaeGI	GKGCMC	1 cut(s)	142
BalI	TGGCCA	1 cut(s)	356
BanI	GGYRCC	1 cut(s)	137
BanII	GRGCYC	3 cut(s)	305, 416, 1153
BauI	CACGAG	1 cut(s)	298
Bbv12I	GWGCWC	2 cut(s)	416, 1153
BbvCI	CCTCAGC	1 cut(s)	1056
BbvI	GCAGC	6 cut(s)	209, 516, 955, 1039, 1204, 1226
BccI	CCATC	8 cut(s)	24, 67, 106, 127, 736, 845, 1130, 1510
BceAI	ACGGC	2 cut(s)	761, 772
BcgI	CGANNNNNNTGC	4 cut(s)	289, 323, 1438, 1472
BciT130I	CCWGG	3 cut(s)	353, 460, 1358
BcnI	CCSGG	2 cut(s)	16, 1318
BcoDI	GTCTC	1 cut(s)	927
BfaI	CTAG	2 cut(s)	930, 1343
BfmI	CTRYAG	2 cut(s)	237, 858
BisI	GCNGC	7 cut(s)	198, 453, 530, 944, 1053, 1215, 1218
BlsI	GCNGC	7 cut(s)	199, 454, 531, 945, 1054, 1216, 1219
Bme1390I	CCNGG	5 cut(s)	16, 353, 460, 1318, 1358
Bme18I	GGWCC	6 cut(s)	545, 841, 1354, 1369, 1399, 1453
BmeT110I	CYCGRG	1 cut(s)	1152
BmgT120I	GGNCC	8 cut(s)	545, 725, 841, 1202, 1354, 1369, 1399, 1453
BmiI	GGNNCC	3 cut(s)	139, 217, 1370
BmrFI	CCNGG	5 cut(s)	16, 353, 460, 1318, 1358
BmsI	GCATC	4 cut(s)	1177, 1199, 1290, 1559
BpmI	CTGGAG	2 cut(s)	244, 1221
Bpu10I	CCTNAGC	1 cut(s)	1056
BpuEI	CTTGAG	1 cut(s)	70
BpuMI	CCSGG	2 cut(s)	16, 1318
BsaJI	CCNNGG	3 cut(s)	357, 459, 788
BsaXI	ACNNNNNCTCC	2 cut(s)	1140, 1170
Bsc4I	CCNNNNNNNGG	4 cut(s)	21, 358, 794, 1343
Bse1I	ACTGG	6 cut(s)	261, 610, 808, 1205, 1328, 1490
Bse3DI	GCAATG	3 cut(s)	305, 1209, 1230
BseBI	CCWGG	3 cut(s)	353, 460, 1358
BseDI	CCNNGG	3 cut(s)	357, 459, 788
BseGI	GGATG	6 cut(s)	35, 117, 182, 1141, 1502, 1550
BseLI	CCNNNNNNNGG	4 cut(s)	21, 358, 794, 1343
BseMI	GCAATG	3 cut(s)	305, 1209, 1230
BseMII	CTCAG	3 cut(s)	656, 672, 1047
BseNI	ACTGG	6 cut(s)	261, 610, 808, 1205, 1328, 1490
BseRI	GAGGAG	2 cut(s)	247, 1560
BseSI	GKGCMC	1 cut(s)	142
BseXI	GCAGC	6 cut(s)	209, 516, 955, 1039, 1204, 1226
BsgI	GTGCAG	3 cut(s)	977, 1058, 1459
BshFI	GGCC	5 cut(s)	356, 437, 727, 1024, 1204
BshNI	GGYRCC	1 cut(s)	137
BsiHKAI	GWGCWC	2 cut(s)	416, 1153
BsiHKCI	CYCGRG	1 cut(s)	1152
BsiSI	CCGG	3 cut(s)	15, 438, 1317
BslI	CCNNNNNNNGG	4 cut(s)	21, 358, 794, 1343
BsmAI	GTCTC	1 cut(s)	927
BsmI	GAATGC	1 cut(s)	522
BsnI	GGCC	5 cut(s)	356, 437, 727, 1024, 1204
BsoBI	CYCGRG	1 cut(s)	1152
Bsp1286I	GDGCHC	4 cut(s)	142, 305, 416, 1153
Bsp1407I	TGTACA	1 cut(s)	741
Bsp143I	GATC	5 cut(s)	99, 177, 228, 374, 1384
Bsp19I	CCATGG	1 cut(s)	357
BspACI	CCGC	1 cut(s)	452
BspANI	GGCC	5 cut(s)	356, 437, 727, 1024, 1204
BspCNI	CTCAG	3 cut(s)	657, 673, 1048
BspLI	GGNNCC	3 cut(s)	139, 217, 1370
BspPI	GGATC	1 cut(s)	1392
BspT107I	GGYRCC	1 cut(s)	137
BsrDI	GCAATG	3 cut(s)	305, 1209, 1230
BsrGI	TGTACA	1 cut(s)	741
BsrI	ACTGG	6 cut(s)	261, 610, 808, 1205, 1328, 1490
BssECI	CCNNGG	3 cut(s)	357, 459, 788
BssMI	GATC	5 cut(s)	99, 177, 228, 374, 1384
BssSI	CACGAG	1 cut(s)	298
BssT1I	CCWWGG	1 cut(s)	357
Bst2BI	CACGAG	1 cut(s)	298
Bst2UI	CCWGG	3 cut(s)	353, 460, 1358
Bst4CI	ACNGT	2 cut(s)	82, 1011
Bst6I	CTCTTC	3 cut(s)	121, 1265, 1507
BstAPI	GCANNNNNTGC	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	741
BstC8I	GCNNGC	6 cut(s)	220, 249, 364, 520, 941, 1251
BstDEI	CTNAG	4 cut(s)	122, 665, 681, 1056
BstDSI	CCRYGG	2 cut(s)	357, 788
BstEII	GGTNACC	1 cut(s)	1312
BstF5I	GGATG	6 cut(s)	35, 117, 182, 1141, 1502, 1550
BstKTI	GATC	5 cut(s)	102, 180, 231, 377, 1387
BstMAI	GTCTC	1 cut(s)	927
BstMBI	GATC	5 cut(s)	99, 177, 228, 374, 1384
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 368, 377, 461, 1021, 1049
BstNI	CCWGG	3 cut(s)	353, 460, 1358
BstNSI	RCATGY	3 cut(s)	345, 366, 967
BstPI	GGTNACC	1 cut(s)	1312
BstSCI	CCNGG	5 cut(s)	14, 351, 458, 1316, 1356
BstSFI	CTRYAG	2 cut(s)	237, 858
BstSLI	GKGCMC	1 cut(s)	142
BstV1I	GCAGC	6 cut(s)	209, 516, 955, 1039, 1204, 1226
BstXI	CCANNNNNNTGG	1 cut(s)	851
BsuRI	GGCC	5 cut(s)	356, 437, 727, 1024, 1204
BtgI	CCRYGG	2 cut(s)	357, 788
BtsCI	GGATG	6 cut(s)	35, 117, 182, 1141, 1502, 1550
BtsIMutI	CAGTG	1 cut(s)	815
Cac8I	GCNNGC	6 cut(s)	220, 249, 364, 520, 941, 1251
CaiI	CAGNNNCTG	2 cut(s)	664, 946
Cfr13I	GGNCC	8 cut(s)	545, 725, 841, 1202, 1354, 1369, 1399, 1453
CsiI	ACCWGGT	1 cut(s)	1356
Csp6I	GTAC	2 cut(s)	742, 924
CviQI	GTAC	2 cut(s)	742, 924
DdeI	CTNAG	4 cut(s)	122, 665, 681, 1056
DpnI	GATC	5 cut(s)	101, 179, 230, 376, 1386
DpnII	GATC	5 cut(s)	99, 177, 228, 374, 1384
EaeI	YGGCCR	1 cut(s)	354
Eam1104I	CTCTTC	3 cut(s)	121, 1265, 1507
EarI	CTCTTC	3 cut(s)	121, 1265, 1507
Ecl136II	GAGCTC	2 cut(s)	414, 1151
Eco130I	CCWWGG	1 cut(s)	357
Eco24I	GRGCYC	3 cut(s)	305, 416, 1153
Eco47I	GGWCC	6 cut(s)	545, 841, 1354, 1369, 1399, 1453
Eco53kI	GAGCTC	2 cut(s)	414, 1151
Eco88I	CYCGRG	1 cut(s)	1152
Eco91I	GGTNACC	1 cut(s)	1312
EcoICRI	GAGCTC	2 cut(s)	414, 1151
EcoO109I	RGGNCCY	2 cut(s)	1354, 1453
EcoO65I	GGTNACC	1 cut(s)	1312
EcoRII	CCWGG	3 cut(s)	351, 458, 1356
EcoT14I	CCWWGG	1 cut(s)	357
EcoT22I	ATGCAT	1 cut(s)	1192
EcoT38I	GRGCYC	3 cut(s)	305, 416, 1153
ErhI	CCWWGG	1 cut(s)	357
FblI	GTMKAC	2 cut(s)	508, 610
Fnu4HI	GCNGC	7 cut(s)	198, 453, 530, 944, 1053, 1215, 1218
FokI	GGATG	6 cut(s)	42, 124, 169, 1148, 1489, 1537
FriOI	GRGCYC	3 cut(s)	305, 416, 1153
Fsp4HI	GCNGC	7 cut(s)	198, 453, 530, 944, 1053, 1215, 1218
FspBI	CTAG	2 cut(s)	930, 1343
GluI	GCNGC	7 cut(s)	198, 453, 530, 944, 1053, 1215, 1218
GsuI	CTGGAG	2 cut(s)	244, 1221
HaeIII	GGCC	5 cut(s)	356, 437, 727, 1024, 1204
HapII	CCGG	3 cut(s)	15, 438, 1317
HincII	GTYRAC	2 cut(s)	611, 1333
HindII	GTYRAC	2 cut(s)	611, 1333
HindIII	AAGCTT	2 cut(s)	46, 908
HinfI	GANTC	4 cut(s)	633, 677, 1424, 1481
HpaII	CCGG	3 cut(s)	15, 438, 1317
HphI	GGTGA	6 cut(s)	109, 161, 995, 1151, 1309, 1372
Hpy166II	GTNNAC	7 cut(s)	281, 509, 611, 742, 805, 1333, 1399
Hpy188I	TCNGA	5 cut(s)	276, 660, 682, 1148, 1389
Hpy188III	TCNNGA	5 cut(s)	103, 553, 701, 781, 1238
Hpy8I	GTNNAC	7 cut(s)	281, 509, 611, 742, 805, 1333, 1399
HpyAV	CCTTC	5 cut(s)	53, 328, 707, 1101, 1384
HpyCH4III	ACNGT	2 cut(s)	82, 1011
HpyCH4V	TGCA	9 cut(s)	92, 251, 994, 1075, 1190, 1214, 1449, 1476, 1531
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 368, 377, 461, 1021, 1049
HpyF3I	CTNAG	4 cut(s)	122, 665, 681, 1056
Kzo9I	GATC	5 cut(s)	99, 177, 228, 374, 1384
LmnI	GCTCC	5 cut(s)	215, 419, 1148, 1240, 1547
Lsp1109I	GCAGC	6 cut(s)	209, 516, 955, 1039, 1204, 1226
LweI	GCATC	4 cut(s)	1177, 1199, 1290, 1559
MabI	ACCWGGT	1 cut(s)	1356
MaeI	CTAG	2 cut(s)	930, 1343
MaeIII	GTNAC	5 cut(s)	76, 293, 1226, 1312, 1430
MalI	GATC	5 cut(s)	101, 179, 230, 376, 1386
MboI	GATC	5 cut(s)	99, 177, 228, 374, 1384
MboII	GAAGA	5 cut(s)	138, 716, 1282, 1451, 1524
MhlI	GDGCHC	4 cut(s)	142, 305, 416, 1153
MlsI	TGGCCA	1 cut(s)	356
MluCI	AATT	7 cut(s)	87, 598, 645, 1018, 1241, 1307, 1523
MluNI	TGGCCA	1 cut(s)	356
MlyI	GAGTC	2 cut(s)	642, 686
MmeI	TCCRAC	2 cut(s)	64, 1113
Mox20I	TGGCCA	1 cut(s)	356
Mph1103I	ATGCAT	1 cut(s)	1192
MscI	TGGCCA	1 cut(s)	356
MseI	TTAA	3 cut(s)	224, 755, 1035
MslI	CAYNNNNRTG	3 cut(s)	147, 1107, 1254
Msp20I	TGGCCA	1 cut(s)	356
MspA1I	CMGCKG	2 cut(s)	946, 1217
MspI	CCGG	3 cut(s)	15, 438, 1317
MspR9I	CCNGG	5 cut(s)	16, 353, 460, 1318, 1358
Mva1269I	GAATGC	1 cut(s)	522
MvaI	CCWGG	3 cut(s)	353, 460, 1358
MwoI	GCNNNNNNNGC	6 cut(s)	248, 368, 377, 461, 1021, 1049
NciI	CCSGG	2 cut(s)	16, 1318
NcoI	CCATGG	1 cut(s)	357
NdeII	GATC	5 cut(s)	99, 177, 228, 374, 1384
NlaIV	GGNNCC	3 cut(s)	139, 217, 1370
NsiI	ATGCAT	1 cut(s)	1192
NspI	RCATGY	3 cut(s)	345, 366, 967
PaeI	GCATGC	1 cut(s)	366
PaeR7I	CTCGAG	1 cut(s)	1152
PctI	GAATGC	1 cut(s)	522
PfeI	GAWTC	2 cut(s)	1424, 1481
PflFI	GACNNNGTC	1 cut(s)	1403
PkrI	GCNGC	7 cut(s)	199, 454, 531, 945, 1054, 1216, 1219
PleI	GAGTC	2 cut(s)	641, 685
PpsI	GAGTC	2 cut(s)	641, 685
PpuMI	RGGWCCY	2 cut(s)	1354, 1453
Psp124BI	GAGCTC	2 cut(s)	416, 1153
Psp5II	RGGWCCY	2 cut(s)	1354, 1453
Psp6I	CCWGG	3 cut(s)	351, 458, 1356
PspEI	GGTNACC	1 cut(s)	1312
PspGI	CCWGG	3 cut(s)	351, 458, 1356
PspN4I	GGNNCC	3 cut(s)	139, 217, 1370
PspPI	GGNCC	8 cut(s)	545, 725, 841, 1202, 1354, 1369, 1399, 1453
PspPPI	RGGWCCY	2 cut(s)	1354, 1453
PspXI	VCTCGAGB	1 cut(s)	1152
PstNI	CAGNNNCTG	2 cut(s)	664, 946
PsyI	GACNNNGTC	1 cut(s)	1403
PvuII	CAGCTG	2 cut(s)	946, 1217
RsaI	GTAC	2 cut(s)	743, 925
RsaNI	GTAC	2 cut(s)	742, 924
RseI	CAYNNNNRTG	3 cut(s)	147, 1107, 1254
SacI	GAGCTC	2 cut(s)	416, 1153
SalI	GTCGAC	1 cut(s)	609
SaqAI	TTAA	3 cut(s)	224, 755, 1035
SatI	GCNGC	7 cut(s)	198, 453, 530, 944, 1053, 1215, 1218
Sau3AI	GATC	5 cut(s)	99, 177, 228, 374, 1384
Sau96I	GGNCC	8 cut(s)	545, 725, 841, 1202, 1354, 1369, 1399, 1453
SchI	GAGTC	2 cut(s)	642, 686
ScrFI	CCNGG	5 cut(s)	16, 353, 460, 1318, 1358
SduI	GDGCHC	4 cut(s)	142, 305, 416, 1153
SexAI	ACCWGGT	1 cut(s)	1356
SfaNI	GCATC	4 cut(s)	1177, 1199, 1290, 1559
SfcI	CTRYAG	2 cut(s)	237, 858
Sfr274I	CTCGAG	1 cut(s)	1152
SinI	GGWCC	6 cut(s)	545, 841, 1354, 1369, 1399, 1453
SlaI	CTCGAG	1 cut(s)	1152
SmiMI	CAYNNNNRTG	3 cut(s)	147, 1107, 1254
SmlI	CTYRAG	2 cut(s)	49, 1152
SmoI	CTYRAG	2 cut(s)	49, 1152
SphI	GCATGC	1 cut(s)	366
Sse9I	AATT	7 cut(s)	87, 598, 645, 1018, 1241, 1307, 1523
SsiI	CCGC	1 cut(s)	452
SspI	AATATT	1 cut(s)	430
SspMI	CTAG	2 cut(s)	930, 1343
SstI	GAGCTC	2 cut(s)	416, 1153
StyD4I	CCNGG	5 cut(s)	14, 351, 458, 1316, 1356
StyI	CCWWGG	1 cut(s)	357
TaaI	ACNGT	2 cut(s)	82, 1011
TaqI	TCGA	7 cut(s)	373, 610, 636, 1153, 1422, 1458, 1498
TaqII	GACCGA	1 cut(s)	533
TasI	AATT	7 cut(s)	87, 598, 645, 1018, 1241, 1307, 1523
TatI	WGTACW	2 cut(s)	741, 923
TauI	GCSGC	1 cut(s)	455
TfiI	GAWTC	2 cut(s)	1424, 1481
Tru1I	TTAA	3 cut(s)	224, 755, 1035
Tru9I	TTAA	3 cut(s)	224, 755, 1035
TscAI	CASTG	1 cut(s)	815
TseI	GCWGC	6 cut(s)	197, 529, 943, 1052, 1214, 1217
TspDTI	ATGAA	4 cut(s)	256, 555, 920, 1091
TspGWI	ACGGA	1 cut(s)	1361
TspRI	CASTG	1 cut(s)	815
Tth111I	GACNNNGTC	1 cut(s)	1403
VpaK11BI	GGWCC	6 cut(s)	545, 841, 1354, 1369, 1399, 1453
XapI	RAATTY	2 cut(s)	645, 1241
XceI	RCATGY	3 cut(s)	345, 366, 967
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1513
XhoI	CTCGAG	1 cut(s)	1152
XmiI	GTMKAC	2 cut(s)	508, 610
XspI	CTAG	2 cut(s)	930, 1343
Zsp2I	ATGCAT	1 cut(s)	1192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.