RLG00000031650

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013762
Physical Location & Seq
Forward (+)
7069240 .. 7069993
754 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031650

Sequence Viewer

Length: 579 bp
ATGTCGGAGACTTTTGCTGATACCAGAGAAGGTCTTGTTGCGATCTCATCCAGGATAACAGAAACATATGCTATGTGTGGAGCTTGCTTAATGATCTTTCCTACAGCTTGGCTTGCAAACCCTTCTAGTTACGTGTTTCAGAGAAAACTTGTGTATGGTAACTCGAGCCCATCACTCGTTAGCATCAAGTTCGTAATCCTCATATCATGTTTCCTCTTGGCCTTGGGATTCTTTGCTTATTCAGCAAAGCAATTCAAGCAAACGCTCTATCTAATAGTCTCACAAGACGAAAGAGATAAGGCCAGGGCAATCAGGGCTTTTATATGGGGAACTACTTTCTTCTCGCTTGGGATCAGATGTCTACTATGTGCTGGCCCTTCGCTACTATGGTTTTTTGGCCCTATCCCCATGTTTGTGTCCTCTGTTGCTTTAGTCATATCCCTCCATGTCGCTGGCAATCGTGCCAGTCCACAATTGCATGTTCATCTTGAGAATACGGATGAAAGTCATGAAGACAATTCCAATATTGCAAAGGAAGAAGCATATATAGAGATGCCCCCTCTTGGTGTTATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

193

Amino Acids

21.3

Weight (kDa)

6.07

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 35 - 149 5.4e-22 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 361
AclWI GGATC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 563
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 1 cut(s) 256
AjnI CCWGG 2 cut(s) 50, 302
AluBI AGCT 2 cut(s) 83, 107
AluI AGCT 2 cut(s) 83, 107
Alw26I GTCTC 2 cut(s) 2, 283
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 4 cut(s) 219, 300, 373, 397
AspS9I GGNCC 2 cut(s) 374, 398
AvaI CYCGRG 1 cut(s) 163
BanII GRGCYC 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 519
BccI CCATC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 2 cut(s) 52, 304
BcoDI GTCTC 2 cut(s) 2, 283
BfaI CTAG 2 cut(s) 126, 577
BfmI CTRYAG 1 cut(s) 102
Bme1390I CCNGG 2 cut(s) 52, 304
BmeT110I CYCGRG 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 374, 398
BmrFI CCNGG 2 cut(s) 52, 304
BmsI GCATC 2 cut(s) 192, 543
BpiI GAAGAC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 509
BsaAI YACGTR 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 222, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse1I ACTGG 1 cut(s) 465
BseBI CCWGG 2 cut(s) 52, 304
BseDI CCNNGG 2 cut(s) 222, 303
BseGI GGATG 2 cut(s) 47, 505
BseLI CCNNNNNNNGG 1 cut(s) 563
BseNI ACTGG 1 cut(s) 465
BshFI GGCC 4 cut(s) 221, 302, 375, 399
BsiHKCI CYCGRG 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 2 cut(s) 2, 283
BsnI GGCC 4 cut(s) 221, 302, 375, 399
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 3 cut(s) 42, 93, 351
BspANI GGCC 4 cut(s) 221, 302, 375, 399
BspHI TCATGA 1 cut(s) 508
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 2 cut(s) 222, 303
BssMI GATC 3 cut(s) 42, 93, 351
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 52, 304
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 4 cut(s) 85, 114, 373, 454
BstF5I GGATG 2 cut(s) 47, 505
BstKTI GATC 3 cut(s) 45, 96, 354
BstMAI GTCTC 2 cut(s) 2, 283
BstMBI GATC 3 cut(s) 42, 93, 351
BstMWI GCNNNNNNNGC 4 cut(s) 113, 242, 256, 314
BstNI CCWGG 2 cut(s) 52, 304
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 50, 302
BstSFI CTRYAG 1 cut(s) 102
BstV2I GAAGAC 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 452
BsuRI GGCC 4 cut(s) 221, 302, 375, 399
BtsCI GGATG 2 cut(s) 47, 505
Cac8I GCNNGC 4 cut(s) 85, 114, 373, 454
CciI TCATGA 1 cut(s) 508
Cfr13I GGNCC 2 cut(s) 374, 398
CviAII CATG 5 cut(s) 207, 409, 446, 479, 509
CviJI RGCY 9 cut(s) 83, 107, 112, 168, 221, 302, 317, 375, 399
CviKI_1 RGCY 9 cut(s) 83, 107, 112, 168, 221, 302, 317, 375, 399
DpnI GATC 3 cut(s) 44, 95, 353
DpnII GATC 3 cut(s) 42, 93, 351
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 163
EcoRII CCWGG 2 cut(s) 50, 302
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 5 cut(s) 210, 412, 449, 482, 512
FatI CATG 5 cut(s) 206, 408, 445, 478, 508
FauNDI CATATG 1 cut(s) 67
FblI GTMKAC 1 cut(s) 361
FokI GGATG 2 cut(s) 34, 512
FriOI GRGCYC 1 cut(s) 170
FspBI CTAG 2 cut(s) 126, 577
HaeIII GGCC 4 cut(s) 221, 302, 375, 399
Hin1II CATG 5 cut(s) 210, 412, 449, 482, 512
HinfI GANTC 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 362, 470
Hpy188I TCNGA 3 cut(s) 7, 141, 356
Hpy188III TCNNGA 2 cut(s) 488, 509
Hpy8I GTNNAC 2 cut(s) 362, 470
HpyAV CCTTC 3 cut(s) 23, 132, 387
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 116, 478, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 242, 256, 314
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 5 cut(s) 210, 412, 449, 482, 512
Kzo9I GATC 3 cut(s) 42, 93, 351
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 9 cut(s) 37, 37, 64, 289, 298, 316, 357, 438, 478
LweI GCATC 2 cut(s) 192, 543
MaeI CTAG 2 cut(s) 126, 577
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 128, 158
MalI GATC 3 cut(s) 44, 95, 353
MboI GATC 3 cut(s) 42, 93, 351
MboII GAAGA 3 cut(s) 331, 524, 548
MfeI CAATTG 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 3 cut(s) 251, 473, 517
MnlI CCTC 5 cut(s) 209, 224, 430, 452, 570
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 413
MspR9I CCNGG 2 cut(s) 52, 304
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 2 cut(s) 52, 304
MwoI GCNNNNNNNGC 4 cut(s) 113, 242, 256, 314
NdeI CATATG 1 cut(s) 67
NdeII GATC 3 cut(s) 42, 93, 351
NlaIII CATG 5 cut(s) 210, 412, 449, 482, 512
NspI RCATGY 1 cut(s) 482
PaeR7I CTCGAG 1 cut(s) 163
PagI TCATGA 1 cut(s) 508
PfeI GAWTC 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 50
Ppu21I YACGTR 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 50, 302
PspGI CCWGG 2 cut(s) 50, 302
PspPI GGNCC 2 cut(s) 374, 398
PspXI VCTCGAGB 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 1 cut(s) 89
Sau3AI GATC 3 cut(s) 42, 93, 351
Sau96I GGNCC 2 cut(s) 374, 398
ScrFI CCNGG 2 cut(s) 52, 304
SduI GDGCHC 1 cut(s) 170
SetI ASST 4 cut(s) 34, 85, 109, 135
SfaNI GCATC 2 cut(s) 192, 543
SfcI CTRYAG 1 cut(s) 102
Sfr274I CTCGAG 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 163, 488
SmoI CTYRAG 2 cut(s) 163, 488
Sse9I AATT 3 cut(s) 251, 473, 517
SspI AATATT 1 cut(s) 526
SspMI CTAG 2 cut(s) 126, 577
StyD4I CCNGG 2 cut(s) 50, 302
StyI CCWWGG 1 cut(s) 222
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 164
TasI AATT 3 cut(s) 251, 473, 517
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TspDTI ATGAA 3 cut(s) 473, 516, 525
TspGWI ACGGA 1 cut(s) 512
XceI RCATGY 1 cut(s) 482
XhoI CTCGAG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 361
XspI CTAG 2 cut(s) 126, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.