RchiOBHm_Chr5g0035201
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013762
Physical Location & Seq
Forward (+)
29096823 .. 29103860
7038 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31411

Sequence Viewer

Length: 1209 bp
ATGGCATGCTTTGCTCAATCAGCAGAGCACTTCACCCAAGCATACTGTCTAATGAGCTCCCAAAACAGCAATATTGAGGCCGGAGATGTGGAGGCGGCAACCGGGGCAGTTTTAGGTGGAGTTCATTTCAAATCTCTTGGGATGAGATGTCTACAATGTGCTGGCATTCTGCTACTATGGTTTTTCGGTCCTATCCCAATGTTTGTTTCCTCTGTGGCTCTAGTCATATTCCTCCATTACAATTATACTGGTCGACAATATCATGTTCGTAATAGAGTCGATTCGCAAATGCAGTCTTTTCAGAAACTGAGGTTTTGTGAGGCTGAGATTTTGTGTGCCCTTTGTGAAGATAATGTGATTGGCAGAGGGCCATCTGGGAAAGTGTACAAGGTTGAGTCAAGCAATGGTACAACTATTGCAGTGAAGAAGCTGCAGAGAAGAAGAGTGAACCAGTGCGAAAACAACGATGTTGAGAAAGATATGGTCCATGATGATGGCCTTATAGCAGGGTTTGACACTTTGGGGAGGATTACGCACAAGAACATTATGAAGCTTTGGCATTGTTGTACTACTAGAGACTGCCAGCAGCTGTTGGTTTATGATTACATGCCAAATGGTAGTCTTGGTGATTTTCTGCACAATAAGAAAGCAGGGTTGCTGAATTGGCCTACAAGGTTTAAGATTGCTCTTGGTGCTGCTGAGGGGATTTCTTATCTGCACCATGATTGTTCTCCTTCTATTGTTCATAGAAATGTGAAATCAAACAATATATTGTTGGATGGTGATTTCGAAGCTCGAGTGGTGGATATTGGAACAACTAATGGCGTTGATGCATCTGGAAAAGGGCCAGTCATTGCAGCTGCTTGTGGTTACATTGCTCCAGAATTTTTGCACGCACATGGGTTGAATGGGAAGAGTGATATATACAACTTTGGTGTGGTGATGCTGGAATTGGTTACCGGGAAACTTCCAGTTAACCCAGAACTAGGAGAAAAGGACCTGGTGAAGTGGGTCCGTAGCACTTTGGATCAGAAAGGAGTGGACCATGTCCTTGACCCCAAGCTCGATTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCGATATTGGGCTTCTCTGCACCGATTCTCGTCCAGACAATCGACCATCCATGAGAAGAGTGATGGAATTGTTGCAAGAAGTGGGCAGAGGAGCATCCCCATATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

402

Amino Acids

44.48

Weight (kDa)

6.69

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 2 - 79 7.9e-12 Protein of unknown function, DUF599
PK_Tyr_Ser-Thr PF07714 117 - 390 4.9e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 118 - 389 2e-33 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 151, 253
AciI CCGC 1 cut(s) 95
AclWI GGATC 1 cut(s) 1035
AcsI RAATTY 1 cut(s) 884
AfaI GTAC 3 cut(s) 386, 409, 568
AfiI CCNNNNNNNGG 1 cut(s) 986
AgsI TTSAA 2 cut(s) 130, 907
AjnI CCWGG 1 cut(s) 999
AjuI GAANNNNNNNTTGG 2 cut(s) 190, 222
AluBI AGCT 7 cut(s) 57, 430, 553, 589, 794, 860, 1063
AluI AGCT 7 cut(s) 57, 430, 553, 589, 794, 860, 1063
Alw21I GWGCWC 2 cut(s) 30, 59
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 1 cut(s) 1035
AlwNI CAGNNNCTG 2 cut(s) 307, 589
Ama87I CYCGRG 1 cut(s) 795
AoxI GGCC 5 cut(s) 78, 368, 496, 665, 845
ApeKI GCWGC 5 cut(s) 430, 586, 695, 857, 860
ApoI RAATTY 1 cut(s) 884
AspS9I GGNCC 8 cut(s) 188, 368, 484, 845, 997, 1012, 1042, 1096
AsuC2I CCSGG 2 cut(s) 103, 961
AsuHPI GGTGA 5 cut(s) 25, 638, 794, 952, 1015
AsuII TTCGAA 1 cut(s) 789
AvaI CYCGRG 1 cut(s) 795
AvaII GGWCC 6 cut(s) 188, 484, 997, 1012, 1042, 1096
BaeGI GKGCMC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 30, 59
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 5 cut(s) 417, 598, 682, 847, 869
BccI CCATC 5 cut(s) 379, 488, 773, 1153, 1156
BcgI CGANNNNNNTGC 2 cut(s) 1081, 1115
BciT130I CCWGG 1 cut(s) 1001
BcnI CCSGG 2 cut(s) 103, 961
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 3 cut(s) 221, 573, 986
BfmI CTRYAG 1 cut(s) 431
BisI GCNGC 6 cut(s) 96, 431, 587, 696, 858, 861
BlsI GCNGC 6 cut(s) 97, 432, 588, 697, 859, 862
Bme1390I CCNGG 3 cut(s) 103, 961, 1001
Bme18I GGWCC 6 cut(s) 188, 484, 997, 1012, 1042, 1096
BmeT110I CYCGRG 1 cut(s) 795
BmgT120I GGNCC 8 cut(s) 188, 368, 484, 845, 997, 1012, 1042, 1096
BmiI GGNNCC 1 cut(s) 1013
BmrFI CCNGG 3 cut(s) 103, 961, 1001
BmsI GCATC 4 cut(s) 820, 842, 933, 1202
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 1 cut(s) 699
Bpu14I TTCGAA 1 cut(s) 789
BpuMI CCSGG 2 cut(s) 103, 961
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 1 cut(s) 986
Bse1I ACTGG 4 cut(s) 253, 451, 848, 971
Bse3DI GCAATG 3 cut(s) 409, 852, 873
BseBI CCWGG 1 cut(s) 1001
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 4 cut(s) 147, 784, 1145, 1193
BseLI CCNNNNNNNGG 1 cut(s) 986
BseMI GCAATG 3 cut(s) 409, 852, 873
BseMII CTCAG 3 cut(s) 299, 315, 690
BseNI ACTGG 4 cut(s) 253, 451, 848, 971
BseRI GAGGAG 1 cut(s) 1203
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 5 cut(s) 417, 598, 682, 847, 869
BsgI GTGCAG 3 cut(s) 620, 701, 1102
BshFI GGCC 5 cut(s) 80, 370, 498, 667, 847
BsiHKAI GWGCWC 2 cut(s) 30, 59
BsiHKCI CYCGRG 1 cut(s) 795
BsiSI CCGG 3 cut(s) 81, 102, 960
BslI CCNNNNNNNGG 1 cut(s) 986
BsmAI GTCTC 1 cut(s) 570
BsmI GAATGC 1 cut(s) 165
BsnI GGCC 5 cut(s) 80, 370, 498, 667, 847
BsoBI CYCGRG 1 cut(s) 795
Bsp119I TTCGAA 1 cut(s) 789
Bsp1286I GDGCHC 3 cut(s) 30, 59, 340
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 1 cut(s) 1027
BspACI CCGC 1 cut(s) 95
BspANI GGCC 5 cut(s) 80, 370, 498, 667, 847
BspCNI CTCAG 3 cut(s) 300, 316, 691
BspLI GGNNCC 1 cut(s) 1013
BspMAI CTGCAG 1 cut(s) 435
BspPI GGATC 1 cut(s) 1035
BspT104I TTCGAA 1 cut(s) 789
BsrDI GCAATG 3 cut(s) 409, 852, 873
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 4 cut(s) 253, 451, 848, 971
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 1 cut(s) 1027
Bst2UI CCWGG 1 cut(s) 1001
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 3 cut(s) 436, 908, 1150
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 384
BstBI TTCGAA 1 cut(s) 789
BstC8I GCNNGC 4 cut(s) 7, 163, 584, 894
BstDEI CTNAG 3 cut(s) 308, 324, 699
BstEII GGTNACC 1 cut(s) 955
BstF5I GGATG 4 cut(s) 147, 784, 1145, 1193
BstKTI GATC 1 cut(s) 1030
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 1 cut(s) 1027
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 104, 664, 692
BstNI CCWGG 1 cut(s) 1001
BstNSI RCATGY 2 cut(s) 9, 610
BstPI GGTNACC 1 cut(s) 955
BstSCI CCNGG 3 cut(s) 101, 959, 999
BstSFI CTRYAG 1 cut(s) 431
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 5 cut(s) 417, 598, 682, 847, 869
BstXI CCANNNNNNTGG 1 cut(s) 494
BsuRI GGCC 5 cut(s) 80, 370, 498, 667, 847
BtsCI GGATG 4 cut(s) 147, 784, 1145, 1193
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 2 cut(s) 426, 458
Cac8I GCNNGC 4 cut(s) 7, 163, 584, 894
CaiI CAGNNNCTG 2 cut(s) 307, 589
Cfr13I GGNCC 8 cut(s) 188, 368, 484, 845, 997, 1012, 1042, 1096
CsiI ACCWGGT 1 cut(s) 999
Csp6I GTAC 3 cut(s) 385, 408, 567
CviAII CATG 8 cut(s) 6, 263, 488, 607, 722, 899, 1046, 1150
CviQI GTAC 3 cut(s) 385, 408, 567
DdeI CTNAG 3 cut(s) 308, 324, 699
DpnI GATC 1 cut(s) 1029
DpnII GATC 1 cut(s) 1027
Eam1104I CTCTTC 3 cut(s) 436, 908, 1150
EarI CTCTTC 3 cut(s) 436, 908, 1150
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 6 cut(s) 188, 484, 997, 1012, 1042, 1096
Eco53kI GAGCTC 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 795
Eco91I GGTNACC 1 cut(s) 955
EcoICRI GAGCTC 1 cut(s) 57
EcoO109I RGGNCCY 2 cut(s) 997, 1096
EcoO65I GGTNACC 1 cut(s) 955
EcoRII CCWGG 1 cut(s) 999
EcoT22I ATGCAT 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 8 cut(s) 9, 266, 491, 610, 725, 902, 1049, 1153
FatI CATG 8 cut(s) 5, 262, 487, 606, 721, 898, 1045, 1149
FblI GTMKAC 2 cut(s) 151, 253
Fnu4HI GCNGC 6 cut(s) 96, 431, 587, 696, 858, 861
FokI GGATG 4 cut(s) 154, 791, 1132, 1180
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 6 cut(s) 96, 431, 587, 696, 858, 861
FspBI CTAG 3 cut(s) 221, 573, 986
GluI GCNGC 6 cut(s) 96, 431, 587, 696, 858, 861
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 5 cut(s) 80, 370, 498, 667, 847
HapII CCGG 3 cut(s) 81, 102, 960
Hin1II CATG 8 cut(s) 9, 266, 491, 610, 725, 902, 1049, 1153
HincII GTYRAC 2 cut(s) 254, 976
HindII GTYRAC 2 cut(s) 254, 976
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 5 cut(s) 276, 281, 395, 1067, 1124
HpaI GTTAAC 1 cut(s) 976
HpaII CCGG 3 cut(s) 81, 102, 960
HphI GGTGA 5 cut(s) 25, 638, 794, 952, 1015
Hpy166II GTNNAC 6 cut(s) 152, 254, 385, 448, 976, 1042
Hpy188I TCNGA 2 cut(s) 303, 1032
Hpy188III TCNNGA 3 cut(s) 837, 881, 1133
Hpy8I GTNNAC 6 cut(s) 152, 254, 385, 448, 976, 1042
HpyAV CCTTC 1 cut(s) 744
HpyCH4III ACNGT 1 cut(s) 47
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 104, 664, 692
HpyF3I CTNAG 3 cut(s) 308, 324, 699
Hsp92II CATG 8 cut(s) 9, 266, 491, 610, 725, 902, 1049, 1153
KspAI GTTAAC 1 cut(s) 976
Kzo9I GATC 1 cut(s) 1027
LmnI GCTCC 3 cut(s) 62, 883, 1190
Lsp1109I GCAGC 5 cut(s) 417, 598, 682, 847, 869
LweI GCATC 4 cut(s) 820, 842, 933, 1202
MabI ACCWGGT 1 cut(s) 999
MaeI CTAG 3 cut(s) 221, 573, 986
MaeIII GTNAC 3 cut(s) 869, 955, 1073
MalI GATC 1 cut(s) 1029
MboI GATC 1 cut(s) 1027
MboII GAAGA 7 cut(s) 359, 436, 450, 453, 925, 1094, 1167
MhlI GDGCHC 3 cut(s) 30, 59, 340
MluCI AATT 5 cut(s) 241, 661, 884, 950, 1166
MlyI GAGTC 2 cut(s) 285, 404
MmeI TCCRAC 1 cut(s) 756
Mph1103I ATGCAT 1 cut(s) 835
MseI TTAA 2 cut(s) 678, 975
MslI CAYNNNNRTG 3 cut(s) 492, 750, 897
MspA1I CMGCKG 2 cut(s) 589, 860
MspI CCGG 3 cut(s) 81, 102, 960
MspR9I CCNGG 3 cut(s) 103, 961, 1001
Mva1269I GAATGC 1 cut(s) 165
MvaI CCWGG 1 cut(s) 1001
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 104, 664, 692
NciI CCSGG 2 cut(s) 103, 961
NdeII GATC 1 cut(s) 1027
NlaIII CATG 8 cut(s) 9, 266, 491, 610, 725, 902, 1049, 1153
NlaIV GGNNCC 1 cut(s) 1013
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 2 cut(s) 9, 610
NspV TTCGAA 1 cut(s) 789
PaeI GCATGC 1 cut(s) 9
PaeR7I CTCGAG 1 cut(s) 795
PctI GAATGC 1 cut(s) 165
PfeI GAWTC 3 cut(s) 281, 1067, 1124
PflFI GACNNNGTC 1 cut(s) 1046
PkrI GCNGC 6 cut(s) 97, 432, 588, 697, 859, 862
PleI GAGTC 2 cut(s) 284, 403
PpsI GAGTC 2 cut(s) 284, 403
PpuMI RGGWCCY 2 cut(s) 997, 1096
Psp124BI GAGCTC 1 cut(s) 59
Psp5II RGGWCCY 2 cut(s) 997, 1096
Psp6I CCWGG 1 cut(s) 999
PspEI GGTNACC 1 cut(s) 955
PspGI CCWGG 1 cut(s) 999
PspN4I GGNNCC 1 cut(s) 1013
PspPI GGNCC 8 cut(s) 188, 368, 484, 845, 997, 1012, 1042, 1096
PspPPI RGGWCCY 2 cut(s) 997, 1096
PspXI VCTCGAGB 1 cut(s) 795
PstI CTGCAG 1 cut(s) 435
PstNI CAGNNNCTG 2 cut(s) 307, 589
PsyI GACNNNGTC 1 cut(s) 1046
PvuII CAGCTG 2 cut(s) 589, 860
RsaI GTAC 3 cut(s) 386, 409, 568
RsaNI GTAC 3 cut(s) 385, 408, 567
RseI CAYNNNNRTG 3 cut(s) 492, 750, 897
SacI GAGCTC 1 cut(s) 59
SalI GTCGAC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 678, 975
SatI GCNGC 6 cut(s) 96, 431, 587, 696, 858, 861
Sau3AI GATC 1 cut(s) 1027
Sau96I GGNCC 8 cut(s) 188, 368, 484, 845, 997, 1012, 1042, 1096
SchI GAGTC 2 cut(s) 285, 404
ScrFI CCNGG 3 cut(s) 103, 961, 1001
SduI GDGCHC 3 cut(s) 30, 59, 340
SexAI ACCWGGT 1 cut(s) 999
SfaNI GCATC 4 cut(s) 820, 842, 933, 1202
SfcI CTRYAG 1 cut(s) 431
Sfr274I CTCGAG 1 cut(s) 795
SfuI TTCGAA 1 cut(s) 789
SinI GGWCC 6 cut(s) 188, 484, 997, 1012, 1042, 1096
SlaI CTCGAG 1 cut(s) 795
SmiMI CAYNNNNRTG 3 cut(s) 492, 750, 897
SmlI CTYRAG 1 cut(s) 795
SmoI CTYRAG 1 cut(s) 795
SphI GCATGC 1 cut(s) 9
Sse9I AATT 5 cut(s) 241, 661, 884, 950, 1166
SsiI CCGC 1 cut(s) 95
SspI AATATT 1 cut(s) 73
SspMI CTAG 3 cut(s) 221, 573, 986
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 3 cut(s) 101, 959, 999
TaaI ACNGT 1 cut(s) 47
TaqI TCGA 7 cut(s) 253, 279, 789, 796, 1065, 1101, 1141
TaqII GACCGA 1 cut(s) 176
TasI AATT 5 cut(s) 241, 661, 884, 950, 1166
TatI WGTACW 2 cut(s) 384, 566
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 3 cut(s) 281, 1067, 1124
Tru1I TTAA 2 cut(s) 678, 975
Tru9I TTAA 2 cut(s) 678, 975
TscAI CASTG 2 cut(s) 426, 458
TseI GCWGC 5 cut(s) 430, 586, 695, 857, 860
TspDTI ATGAA 3 cut(s) 113, 563, 734
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 2 cut(s) 426, 458
Tth111I GACNNNGTC 1 cut(s) 1046
VpaK11BI GGWCC 6 cut(s) 188, 484, 997, 1012, 1042, 1096
XapI RAATTY 1 cut(s) 884
XceI RCATGY 2 cut(s) 9, 610
XhoI CTCGAG 1 cut(s) 795
XmiI GTMKAC 2 cut(s) 151, 253
XspI CTAG 3 cut(s) 221, 573, 986
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.