RchiOBHm_Chr5g0049171

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013644
Physical Location & Seq
Forward (+)
46691841 .. 46695537
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32684

Sequence Viewer

Length: 2952 bp
ATGGAGGAAATAGGCACTGCCACCACCGACTTTCGGGTACTCATCCCTCATTCGAATTCTCAGCACTTTGTCTTGGGTCCAGCACTCAAATCTGACAATAGGTTCCCTGACATTTATAGTGAATTTCCCAAAAGGAAAACCGAAGATTTTCTTGTACATGATCAAAATGCCAAGTTCTATGAATGGTTTCATCAAAAGCATCGGTTTTGGCCTGGCAATGATCCTGATTGGGTCTCTTGGTTTAAAAGAGTTGAAGCTCAAAAGAGTTATGTTTGGCATCAAGCTGGGATCTATGACATTCTCCAGTTCTCTAAGTTTACCATTCCTCTTAATCGTTCTTTAGTACTAGCTGCACTCTGCTTTTGGTCAATTTCCACAAATTCTTTTCATTTTGGTCATGGTCCCCTTACTATTACTCTCTTGGATCTTAGTGCTCTGGTAGGACTTAGACCATGTGGTGCCCAATTTGATGCTGTCGTCGCTGATTCCATCTCCATCCCGGAGGACAATTATGGCATTGAAGTTAATCAGTTCTCCAGTTTTCCTTCATTTATTAGGGAACATAACAATATTGAAGGAGCTGTGACAGATGAAGAGCATGTAGCATTTCTTCTCTATTGGCTTTGCCGTTACCTTCTTTGTTCTCCATCATTCAAGATCATCAAAACCAATTTGCCTTTGGCTGTGGCTTTGTCTAGGGGTGAGTCGTACGCCTTAGGACCCCTGGTTCTTGCCTACTTGTATAGAGGCATCAAGGACTTGCTGCACTCTCAGCTAAATACTACAGGTGGACCAATATGGATTCTTCAACTTTGGCTTCATGCATACTTTCCCAAAATGCGTCCAGATTGTTTTGCAATTCCCAACCTCTCCTGTCATGGACTAAAATATGTTTCTTTCAAACAGCCTAAACTTTTGTTTCATGATTGTTTATTGTATATGAGCTACTTAGACAGAGAAGAGAATTTCAACTTCAATCCATATTCAAAGTTAGATGCTTTACCTCCATGGTTAATATTTCCTTGGACTGAAATGACGAAAACTGAATTTTCTACACTCAAGGACATGTGGGCTAGCTTTCTTGTTAGTCGTGATCTTCCTGTAGGTATAGCTCTTCCAAAATTTGGAAACAAAACGTTTGGTTTTGAAGCATATTGCCCAAATCTTTGTGCACGCCAGTTCGGGCTGATTCAAGGCATTCCTACTCCCCCTTTCTTCTCTCTTAATACGCATTTCCCAGAAAAGATGGTTGTTCGCAGTTTTGAAGAACAACTCCAGTTGCTTAAAGTTACTACAGCTCATTTGTTCACAAAACTTGAACTTTCTTCATATTCACCACTACCTAGTACCGCCTCATCCTTCAAAGCTTGGTGGACCAAATACTATAAGCACACCTTTCCTCAAGATTTGAACTATCTTGAAAAGCTTGATGACTTGACGCGAAACAGAACGCCTCGCCAAGAGCCATCAGAGAAAGCAAGAAAGAATGCTTTGAGGAAGCGCAAAGGGATAATCACCATTCAAGACCCCAGTTCTCACCGACAGCGAGTACCAGACCCTGGTACATCAAGAAAAAGGATTAGGTATGTAACTAAGCAAGGAATCTGTTCAAGTGTGACGGACGGAAAAGGAATGCTGCCTATGATTCAATGCCTACGTGGACAGGGCAGAGTTCATAGCAAGACAAGAATGACTATTAAAGCATGCCATATTTTGAGAAGTATAAGAAAGGGAAATTATTTATGTCACACCAAGTTAGAGGAAGCTGCTACTAAGACAAAGAGGTCGACAGTGTGTAAGAACAAACAAGCTAATTGCTCACCTAAAAATCAATTCCAGAGTGGGCAAGGGAAAAGTTCTGGCAACACACCGAAAGGAGAAGTGCATTGTTATTTGATAAATCTGACACATTCCCATTCTCAGTCTCTCTCTGAGGCCACCAAAGCCGCCTTCCAAAAGAAAACATGTCCTCAGTCAAAGCATCAAGACTCTGATACTGCATGTGGACTCGTTGTTGAATCAACTGAAGATAACATTAATTTTCAGGTTAATGATCCTCCACTAACAGAGACTATGGATGCTTCAAATGAGAAGCTACCTGGTCAAATTTCATCTCCGCCAACAGAAGCAGCGGAGCCTCAGACTCCTCAAGAGCCAAGCATTCAATTCCCATTCCATGCACCTTCATCTCATCCCAACCCTTCTAGTTCTCATACCATACGTGACCCTGAGGTGTCAAATCCTACAAATTCTATACGAGCTTTGGAGTTTTTGGAACGTTATCTACACATGCATTCAGATATTGGTATCAAAACTGAAGAAACTCCCATTCCTAAATCTGAAGAAATTTCTCAAGCCAAGGTCAAGATCCAAAGTATTTCAACACTTCCTCTGGATAAAGTCATGGAAACTGAATGTTTGCAAAGCTTCAAATCATCACTGGAAACTTTGCTCAGATCTAATGACCAAACTGCAGTGCAGTTGGCAAACATTGAAGATGTCCTGAGAAAGCTTGCTTTTCTGGAGTCCAAGTACCCCTCAGCTAGTGGAATCACAGCTGAAGTTAACAAAGCATTTGTTCAGAAAGTACAGCTGGAACAGGAAGTGGAAGTCTACACTGGCCAAATTTCTGAAGCTAAAAAAGAAGAGGCAAAGTTTTTCGAGTGTGTAAGTATACAGAAGTCGAGAGTTGAGGAACTTGAGACAAAATTGGCTGAAGAGAAAGCTACTTTAATTGCATTGGAGAAGTACCATGCCACCTACAAAAGCAAAATGGATGAATTGAATTACAAGTGTGAGCAAGTGCAGCAGATGCTCTGTCATCATAGTATTAATGAGAGAACTTTGGTGAACAAAGCTTTGCAAGCAAGAGGTGATTTTAAATCGATAGAAGCAACTTGGGTTCAGATCCAGCATCTTGTTGCCACAATTGGTGGCAGCAAGCGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

983

Amino Acids

111.83

Weight (kDa)

8.6

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 96 - 406 8.2e-45 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g26742 FvH4_3g26742
malus_domestica MD11G1175200.v1.1
pyrus_communis pycom11g15030
rosa_chinensis RchiOBHm_Chr5g0049171
rosa_laevigata RLG00000034596
rosa_multiflora Rmu_sc0001677.1_g000029
rosa_roxburghii Rroxscaffold_1G00032560
rosa_rugosa Rorug05G0244500 Rorug05G0244600 Rorug05G0244700 Rorug05G0244800 Rorug05G0244900
rosa_samantha Rh5AG323200 Rh5BG333700 Rh5CG358800 Rh5DG345200
rosa_wichuraiana Rw5G030510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1783
AccB1I GGYRCC 1 cut(s) 458
AccB7I CCANNNNNTGG 2 cut(s) 1124, 1559
AccI GTMKAC 3 cut(s) 1787, 2613, 2674
AccII CGCG 1 cut(s) 1441
AciI CCGC 5 cut(s) 1350, 1947, 2117, 2132, 2944
AclI AACGTT 2 cut(s) 1136, 2278
AclWI GGATC 6 cut(s) 215, 296, 432, 2048, 2362, 2902
AcoI YGGCCR 1 cut(s) 2620
AcuI CTGAAG 6 cut(s) 2046, 2337, 2361, 2580, 2652, 2736
AfiI CCNNNNNNNGG 3 cut(s) 33, 1124, 1559
AflIII ACRYGT 2 cut(s) 1065, 1964
AjnI CCWGG 4 cut(s) 211, 723, 1558, 2098
AjuI GAANNNNNNNTTGG 2 cut(s) 1934, 1966
AloI GAACNNNNNNTCC 2 cut(s) 711, 743
Alw21I GWGCWC 2 cut(s) 436, 1174
Alw26I GTCTC 4 cut(s) 238, 1929, 2063, 2696
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 6 cut(s) 215, 296, 432, 2048, 2362, 2902
AlwNI CAGNNNCTG 1 cut(s) 1559
AoxI GGCC 3 cut(s) 209, 1935, 2620
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 7 cut(s) 350, 763, 1636, 1766, 2129, 2806, 2937
AseI ATTAAT 2 cut(s) 2037, 2832
Asp700I GAANNNNTTC 3 cut(s) 147, 186, 1606
AspLEI GCGC 1 cut(s) 1503
AspS9I GGNCC 5 cut(s) 77, 401, 719, 791, 1374
AsuC2I CCSGG 1 cut(s) 500
AsuHPI GGTGA 7 cut(s) 713, 1326, 1507, 1529, 1812, 2860, 2885
AsuII TTCGAA 1 cut(s) 53
AsuNHI GCTAGC 1 cut(s) 1073
AvaII GGWCC 5 cut(s) 77, 401, 719, 791, 1374
AxyI CCTNAGG 2 cut(s) 715, 2229
BaeGI GKGCMC 2 cut(s) 463, 1174
BalI TGGCCA 1 cut(s) 2622
BanI GGYRCC 1 cut(s) 458
BarI GAAGNNNNNNTAC 2 cut(s) 594, 626
Bbv12I GWGCWC 2 cut(s) 436, 1174
BbvCI CCTCAGC 1 cut(s) 2539
BbvI GCAGC 6 cut(s) 337, 750, 1623, 1753, 2141, 2818
BccI CCATC 5 cut(s) 497, 503, 655, 1240, 1474
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 4 cut(s) 213, 725, 1560, 2100
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 1 cut(s) 500
BcoDI GTCTC 4 cut(s) 238, 1929, 2063, 2696
BfaI CTAG 6 cut(s) 347, 696, 1074, 1344, 2205, 2544
BfmI CTRYAG 4 cut(s) 783, 1101, 1293, 2472
BglII AGATCT 1 cut(s) 2456
BisI GCNGC 8 cut(s) 351, 764, 1637, 1767, 1947, 2130, 2807, 2938
BlsI GCNGC 8 cut(s) 352, 765, 1638, 1768, 1948, 2131, 2808, 2939
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 5 cut(s) 213, 500, 725, 1560, 2100
Bme18I GGWCC 5 cut(s) 77, 401, 719, 791, 1374
BmgT120I GGNCC 5 cut(s) 77, 401, 719, 791, 1374
BmiI GGNNCC 6 cut(s) 78, 104, 403, 460, 721, 2136
BmrFI CCNGG 5 cut(s) 213, 500, 725, 1560, 2100
BmrI ACTGGG 1 cut(s) 1524
BmsI GCATC 9 cut(s) 208, 286, 460, 759, 985, 1990, 2068, 2802, 2923
BmtI GCTAGC 1 cut(s) 1077
BmuI ACTGGG 1 cut(s) 1524
BpmI CTGGAG 4 cut(s) 287, 520, 1259, 2543
Bpu10I CCTNAGC 1 cut(s) 2539
Bpu14I TTCGAA 1 cut(s) 53
BpuEI CTTGAG 5 cut(s) 1043, 1386, 2133, 2337, 2720
BpuMI CCSGG 1 cut(s) 500
Bsa29I ATCGAT 1 cut(s) 2885
BsaAI YACGTR 2 cut(s) 1658, 2222
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 5 cut(s) 723, 1007, 1022, 1558, 2358
BsaXI ACNNNNNCTCC 2 cut(s) 2516, 2546
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 1124, 1559
Bse1I ACTGG 7 cut(s) 304, 537, 1177, 1276, 1530, 2445, 2623
Bse21I CCTNAGG 2 cut(s) 715, 2229
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 4 cut(s) 213, 725, 1560, 2100
BseCI ATCGAT 1 cut(s) 2885
BseDI CCNNGG 5 cut(s) 723, 1007, 1022, 1558, 2358
BseGI GGATG 6 cut(s) 42, 495, 1355, 2083, 2191, 2782
BseLI CCNNNNNNNGG 3 cut(s) 33, 1124, 1559
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 7 cut(s) 304, 537, 1177, 1276, 1530, 2445, 2623
BseRI GAGGAG 1 cut(s) 2136
BseSI GKGCMC 2 cut(s) 463, 1174
BseXI GCAGC 6 cut(s) 337, 750, 1623, 1753, 2141, 2818
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 4 cut(s) 336, 749, 2498, 2825
Bsh1236I CGCG 1 cut(s) 1441
BshFI GGCC 3 cut(s) 211, 1937, 2622
BshNI GGYRCC 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 2885
BsiHKAI GWGCWC 2 cut(s) 436, 1174
BsiSI CCGG 1 cut(s) 500
BsiWI CGTACG 1 cut(s) 708
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 3 cut(s) 33, 1124, 1559
BsmAI GTCTC 4 cut(s) 238, 1929, 2063, 2696
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 5 cut(s) 1197, 1492, 1638, 2160, 2293
BsnI GGCC 3 cut(s) 211, 1937, 2622
Bso31I GGTCTC 1 cut(s) 238
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 3 cut(s) 436, 463, 1174
Bsp1407I TGTACA 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 1007
BspACI CCGC 5 cut(s) 1350, 1947, 2117, 2132, 2944
BspANI GGCC 3 cut(s) 211, 1937, 2622
BspDI ATCGAT 1 cut(s) 2885
BspFNI CGCG 1 cut(s) 1441
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 6 cut(s) 78, 104, 403, 460, 721, 2136
BspMAI CTGCAG 1 cut(s) 2476
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 6 cut(s) 215, 296, 432, 2048, 2362, 2902
BspQI GCTCTTC 2 cut(s) 588, 1119
BspT104I TTCGAA 1 cut(s) 53
BspT107I GGYRCC 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 7 cut(s) 304, 537, 1177, 1276, 1530, 2445, 2623
BssECI CCNNGG 5 cut(s) 723, 1007, 1022, 1558, 2358
BssNAI GTATAC 1 cut(s) 2675
BssT1I CCWWGG 3 cut(s) 1007, 1022, 2358
Bst1107I GTATAC 1 cut(s) 2675
Bst2UI CCWGG 4 cut(s) 213, 725, 1560, 2100
Bst4CI ACNGT 1 cut(s) 1792
Bst6I CTCTTC 5 cut(s) 588, 954, 1119, 2640, 2712
BstAPI GCANNNNNTGC 1 cut(s) 2812
BstAUI TGTACA 1 cut(s) 154
BstBAI YACGTR 2 cut(s) 1658, 2222
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 6 cut(s) 1075, 1174, 1705, 2514, 2865, 2942
BstDSI CCRYGG 1 cut(s) 1007
BstF5I GGATG 6 cut(s) 42, 495, 1355, 2083, 2191, 2782
BstFNI CGCG 1 cut(s) 1441
BstHHI GCGC 1 cut(s) 1503
BstMAI GTCTC 4 cut(s) 238, 1929, 2063, 2696
BstMWI GCNNNNNNNGC 6 cut(s) 479, 772, 1943, 2806, 2812, 2864
BstNI CCWGG 4 cut(s) 213, 725, 1560, 2100
BstNSI RCATGY 6 cut(s) 602, 1069, 1707, 1968, 2004, 2293
BstSCI CCNGG 5 cut(s) 211, 498, 723, 1558, 2098
BstSFI CTRYAG 4 cut(s) 783, 1101, 1293, 2472
BstSLI GKGCMC 2 cut(s) 463, 1174
BstUI CGCG 1 cut(s) 1441
BstV1I GCAGC 6 cut(s) 337, 750, 1623, 1753, 2141, 2818
BstX2I RGATCY 5 cut(s) 288, 424, 2367, 2456, 2907
BstYI RGATCY 5 cut(s) 288, 424, 2367, 2456, 2907
BstZ17I GTATAC 1 cut(s) 2675
Bsu15I ATCGAT 1 cut(s) 2885
Bsu36I CCTNAGG 2 cut(s) 715, 2229
BsuRI GGCC 3 cut(s) 211, 1937, 2622
BsuTUI ATCGAT 1 cut(s) 2885
BtgI CCRYGG 1 cut(s) 1007
BtsCI GGATG 6 cut(s) 42, 495, 1355, 2083, 2191, 2782
BtsI GCAGTG 2 cut(s) 15, 2481
BtsIMutI CAGTG 5 cut(s) 15, 1797, 2438, 2481, 2616
Cac8I GCNNGC 6 cut(s) 1075, 1174, 1705, 2514, 2865, 2942
CaiI CAGNNNCTG 1 cut(s) 1559
CciI TCATGA 1 cut(s) 922
CfoI GCGC 1 cut(s) 1503
Cfr13I GGNCC 5 cut(s) 77, 401, 719, 791, 1374
ClaI ATCGAT 1 cut(s) 2885
CseI GACGC 2 cut(s) 830, 1447
CsiI ACCWGGT 1 cut(s) 2098
DraI TTTAAA 2 cut(s) 244, 2881
DrdI GACNNNNNNGTC 1 cut(s) 1783
DseDI GACNNNNNNGTC 1 cut(s) 1783
EaeI YGGCCR 1 cut(s) 2620
Eam1104I CTCTTC 5 cut(s) 588, 954, 1119, 2640, 2712
EarI CTCTTC 5 cut(s) 588, 954, 1119, 2640, 2712
EciI GGCGGA 1 cut(s) 2106
Eco130I CCWWGG 3 cut(s) 1007, 1022, 2358
Eco31I GGTCTC 1 cut(s) 238
Eco47I GGWCC 5 cut(s) 77, 401, 719, 791, 1374
Eco57I CTGAAG 6 cut(s) 2046, 2337, 2361, 2580, 2652, 2736
Eco81I CCTNAGG 2 cut(s) 715, 2229
EcoO109I RGGNCCY 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 4 cut(s) 211, 723, 1558, 2098
EcoT14I CCWWGG 3 cut(s) 1007, 1022, 2358
EcoT22I ATGCAT 2 cut(s) 826, 2295
ErhI CCWWGG 3 cut(s) 1007, 1022, 2358
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1379, 1411
FaqI GGGAC 1 cut(s) 387
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 3 cut(s) 1787, 2613, 2674
Fnu4HI GCNGC 8 cut(s) 351, 764, 1637, 1767, 1947, 2130, 2807, 2938
FokI GGATG 6 cut(s) 29, 482, 1342, 2090, 2178, 2789
Fsp4HI GCNGC 8 cut(s) 351, 764, 1637, 1767, 1947, 2130, 2807, 2938
FspBI CTAG 6 cut(s) 347, 696, 1074, 1344, 2205, 2544
GlaI GCGC 1 cut(s) 1502
GluI GCNGC 8 cut(s) 351, 764, 1637, 1767, 1947, 2130, 2807, 2938
GsaI CCCAGC 1 cut(s) 288
GsuI CTGGAG 4 cut(s) 287, 520, 1259, 2543
HaeIII GGCC 3 cut(s) 211, 1937, 2622
HapII CCGG 1 cut(s) 500
HgaI GACGC 2 cut(s) 830, 1447
HhaI GCGC 1 cut(s) 1503
Hin6I GCGC 1 cut(s) 1501
HinP1I GCGC 1 cut(s) 1501
HincII GTYRAC 2 cut(s) 1788, 2566
HindII GTYRAC 2 cut(s) 1788, 2566
HindIII AAGCTT 5 cut(s) 1365, 1424, 2425, 2510, 2856
HpaI GTTAAC 1 cut(s) 2566
HpaII CCGG 1 cut(s) 500
HphI GGTGA 7 cut(s) 713, 1326, 1507, 1529, 1812, 2860, 2885
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 7 cut(s) 555, 569, 644, 1369, 1960, 2193, 2211
HpyCH4III ACNGT 1 cut(s) 1792
HpyCH4IV ACGT 4 cut(s) 1136, 1657, 2221, 2278
HpyF10VI GCNNNNNNNGC 6 cut(s) 479, 772, 1943, 2806, 2812, 2864
HpySE526I ACGT 4 cut(s) 1136, 1657, 2221, 2278
HspAI GCGC 1 cut(s) 1501
Ksp22I TGATCA 1 cut(s) 160
KspAI GTTAAC 1 cut(s) 2566
LguI GCTCTTC 2 cut(s) 588, 1119
LmnI GCTCC 2 cut(s) 578, 2134
Lsp1109I GCAGC 6 cut(s) 337, 750, 1623, 1753, 2141, 2818
LweI GCATC 9 cut(s) 208, 286, 460, 759, 985, 1990, 2068, 2802, 2923
MabI ACCWGGT 1 cut(s) 2098
MaeI CTAG 6 cut(s) 347, 696, 1074, 1344, 2205, 2544
MaeII ACGT 4 cut(s) 1136, 1657, 2221, 2278
MaeIII GTNAC 7 cut(s) 583, 629, 1288, 1588, 1615, 1745, 2222
MfeI CAATTG 1 cut(s) 2928
MflI RGATCY 5 cut(s) 288, 424, 2367, 2456, 2907
MhlI GDGCHC 3 cut(s) 436, 463, 1174
MlsI TGGCCA 1 cut(s) 2622
MluNI TGGCCA 1 cut(s) 2622
MlyI GAGTC 5 cut(s) 713, 1982, 2001, 2137, 2534
Mox20I TGGCCA 1 cut(s) 2622
Mph1103I ATGCAT 2 cut(s) 826, 2295
MroXI GAANNNNTTC 3 cut(s) 147, 186, 1606
MscI TGGCCA 1 cut(s) 2622
Msp20I TGGCCA 1 cut(s) 2622
MspA1I CMGCKG 3 cut(s) 2132, 2558, 2593
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 5 cut(s) 213, 500, 725, 1560, 2100
MunI CAATTG 1 cut(s) 2928
Mva1269I GAATGC 5 cut(s) 1197, 1492, 1638, 2160, 2293
MvaI CCWGG 4 cut(s) 213, 725, 1560, 2100
MvnI CGCG 1 cut(s) 1441
MwoI GCNNNNNNNGC 6 cut(s) 479, 772, 1943, 2806, 2812, 2864
NciI CCSGG 1 cut(s) 500
NcoI CCATGG 1 cut(s) 1007
NheI GCTAGC 1 cut(s) 1073
NlaIV GGNNCC 6 cut(s) 78, 104, 403, 460, 721, 2136
NmuCI GTSAC 4 cut(s) 583, 1615, 1745, 2222
NsiI ATGCAT 2 cut(s) 826, 2295
NspI RCATGY 6 cut(s) 602, 1069, 1707, 1968, 2004, 2293
NspV TTCGAA 1 cut(s) 53
PaeI GCATGC 1 cut(s) 1707
PagI TCATGA 1 cut(s) 922
PciI ACATGT 2 cut(s) 1065, 1964
PciSI GCTCTTC 2 cut(s) 588, 1119
PctI GAATGC 5 cut(s) 1197, 1492, 1638, 2160, 2293
PdmI GAANNNNTTC 3 cut(s) 147, 186, 1606
PfeI GAWTC 7 cut(s) 485, 802, 1189, 1602, 1645, 2018, 2550
Pfl23II CGTACG 1 cut(s) 708
PflMI CCANNNNNTGG 2 cut(s) 1124, 1559
PfoI TCCNGGA 1 cut(s) 498
PkrI GCNGC 8 cut(s) 352, 765, 1638, 1768, 1948, 2131, 2808, 2939
PleI GAGTC 5 cut(s) 712, 1982, 2001, 2137, 2533
PpsI GAGTC 5 cut(s) 712, 1982, 2001, 2137, 2533
Ppu21I YACGTR 2 cut(s) 1658, 2222
PpuMI RGGWCCY 1 cut(s) 719
PscI ACATGT 2 cut(s) 1065, 1964
PshBI ATTAAT 2 cut(s) 2037, 2832
Psp1406I AACGTT 2 cut(s) 1136, 2278
Psp5II RGGWCCY 1 cut(s) 719
Psp6I CCWGG 4 cut(s) 211, 723, 1558, 2098
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 4 cut(s) 211, 723, 1558, 2098
PspLI CGTACG 1 cut(s) 708
PspN4I GGNNCC 6 cut(s) 78, 104, 403, 460, 721, 2136
PspPI GGNCC 5 cut(s) 77, 401, 719, 791, 1374
PspPPI RGGWCCY 1 cut(s) 719
PsrI GAACNNNNNNTAC 2 cut(s) 2268, 2300
PstI CTGCAG 1 cut(s) 2476
PstNI CAGNNNCTG 1 cut(s) 1559
PsuI RGATCY 5 cut(s) 288, 424, 2367, 2456, 2907
PvuII CAGCTG 2 cut(s) 2558, 2593
SalI GTCGAC 1 cut(s) 1786
SapI GCTCTTC 2 cut(s) 588, 1119
SatI GCNGC 8 cut(s) 351, 764, 1637, 1767, 1947, 2130, 2807, 2938
Sau96I GGNCC 5 cut(s) 77, 401, 719, 791, 1374
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 5 cut(s) 713, 1982, 2001, 2137, 2534
ScrFI CCNGG 5 cut(s) 213, 500, 725, 1560, 2100
SduI GDGCHC 3 cut(s) 436, 463, 1174
SexAI ACCWGGT 1 cut(s) 2098
SfaNI GCATC 9 cut(s) 208, 286, 460, 759, 985, 1990, 2068, 2802, 2923
SfcI CTRYAG 4 cut(s) 783, 1101, 1293, 2472
SfuI TTCGAA 1 cut(s) 53
SinI GGWCC 5 cut(s) 77, 401, 719, 791, 1374
SmlI CTYRAG 5 cut(s) 1058, 1401, 2148, 2352, 2699
SmoI CTYRAG 5 cut(s) 1058, 1401, 2148, 2352, 2699
SphI GCATGC 1 cut(s) 1707
SsiI CCGC 5 cut(s) 1350, 1947, 2117, 2132, 2944
SspI AATATT 2 cut(s) 571, 1017
SspMI CTAG 6 cut(s) 347, 696, 1074, 1344, 2205, 2544
StyD4I CCNGG 5 cut(s) 211, 498, 723, 1558, 2098
StyI CCWWGG 3 cut(s) 1007, 1022, 2358
TaaI ACNGT 1 cut(s) 1792
TaiI ACGT 4 cut(s) 1139, 1660, 2224, 2281
TaqI TCGA 5 cut(s) 53, 1787, 2661, 2684, 2885
TatI WGTACW 3 cut(s) 154, 343, 2587
TauI GCSGC 1 cut(s) 1949
TfiI GAWTC 7 cut(s) 485, 802, 1189, 1602, 1645, 2018, 2550
TscAI CASTG 5 cut(s) 22, 1797, 2445, 2481, 2623
TseFI GTSAC 4 cut(s) 583, 1615, 1745, 2222
TseI GCWGC 7 cut(s) 350, 763, 1636, 1766, 2129, 2806, 2937
Tsp45I GTSAC 4 cut(s) 583, 1615, 1745, 2222
TspGWI ACGGA 2 cut(s) 1634, 1638
TspRI CASTG 5 cut(s) 22, 1797, 2445, 2481, 2623
Van91I CCANNNNNTGG 2 cut(s) 1124, 1559
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 5 cut(s) 77, 401, 719, 791, 1374
VspI ATTAAT 2 cut(s) 2037, 2832
XceI RCATGY 6 cut(s) 602, 1069, 1707, 1968, 2004, 2293
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XmiI GTMKAC 3 cut(s) 1787, 2613, 2674
XmnI GAANNNNTTC 3 cut(s) 147, 186, 1606
XspI CTAG 6 cut(s) 347, 696, 1074, 1344, 2205, 2544
ZrmI AGTACT 1 cut(s) 345
Zsp2I ATGCAT 2 cut(s) 826, 2295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.