Rroxscaffold_1G00032560

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013644
Physical Location & Seq
Reverse (-)
46605640 .. 46609133
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00032560.1

Sequence Viewer

Length: 3183 bp
ATGGCATCCTCTAGAGATGATGCATTGGCCACCATGGCTGTCAATAGAGTGAATCAACTTATGGAGGAAATAGGCACTGCAACCACCGACTTTCGGGTACTCATCCCTCATTCGAATTCTCAGCACTTTGCCTTAGGTCCAGCACTCAAATCTGACAATAGGTTCCCTGACATTTATAGTGAATTTCCCAAAAGGAAAACTGAAGATTTTCTTATACATGATCAAAATGCCAAGTTCTATGAATGGTTTCATCAAAAGCATCGGTTTTGGCCTGGCAATGATCCTGATTGGGTCTCTTGGTTTAAAAGAGTTGAAGCTCAAAAGAGTTATGTTTGGCATCAAGCTGGGATCTATGACATTCTCCAGTTCTCTAAGTTTACCATTCCTCTTAATCGTTCTTTAGTACTAGCTGCACTCTGCTTTTGGTCAATTTCCACAAATTCTTTTCATTTTGGTCTTGGTCCCCTTACTATTACTCTCTTGGATCTTAGTGCTCTGGTGGGACTTAGACCATGTGGTGCCCAATTTGATGCTGTCGTCGCTGATTCCATATCCATCCCGGAGGATGATTATGGCATTGAAGTTAATCAGTTCTCCAGTTTTCCTTCATTTATTAGGGAACATAACAATATTGAAGGAGCTGTGACAGATGAAGAGCATGTAGCATTTCTTCACTATTGGCTTTGCCGTTACCTTCTTTGTTCTCCATCATTCAAGATCATCAAAACCAATTTGCCTTTGGCTGTGGCTTTGTCTAGGGGTGAGTCGTACGCCTTAGGACCCCTGGTTCTTGCCTACTTGTATAGAGGCATCAAGGACTTGCTGCATTCTCAGCTAAATACTACAGGTGGACCAATATGGATTCTTCAACTTTGGCTTCATGCATACTTTCCCAAAATGCGTCCAGATTGTTTTGCAATTCCCAACCTCTCCTGTCATGGACTAAAATATGTTTCTTTCAAACAGCCTGAACTTTTGTTTCATGATTGTTTATTGTATATGAGCTACTTAGACAGAGAAGAGAATTTCAACTTCAATCCATATTCAAAGTTAGATGCTTTACCTCCATGGTTAATATTTCCTTGGACTGAAATGACGGAAACTGAATTTTCTACAATCAAGGACATGTGGGCTAGCTTTCTTGTTAGTCGTGATCTTCCTGTAGGTATAGCTCTTCCAAAATTTGGAAACAAAACGTTTGGTTTTGAAGCATATTGCCCAAATCTTTGTGCACGCCAGTTTGGGCTGATTCAAGGCATTCCTACTCCCCCTTTCTTCTCTCTTAATACCCATTTCCCAGAAAAGATAGTTGTTCGCAGTTTTGAAGAACAACTACAGGTGCTTAAAGTTACTACAGCTCATTTGTTCACAAAACTTGAACTTTCTTCATATTCACCACTACCTAGTACCACCTCATCCTTCAAAGCTTGGTGGACCAAATACTATAAGCACACCTTTCCTCAAGATTTGAACTATCTTGAAAAGCTTGATGACTTGACCCGAAACAGAACCCCTCGCCAAGAGCCATCAGAGAAAGCAAGAAAGAATGCTTTGAGGAAGCGCAAAGGTATTTCGGCCCCCATCTACTTTGTCTATTCATTTGACAGAATTTATCAGATGAAGTATGATGTTGATGCTGTAGCTATAACAAGTTCAACTGAGTTGGTCAACCTCATTATGGTTAAGCCTTCAAGGGCAGTTATTGAGGTTGGATTCCAGTGGCTGATTAGGACAGGGGTAATCACCATTCAAGACCCCAGTTCTCACCAACAGCGAGTACTAGACCCTGGTACATCAAGAAAAAGGATTAGGTATGTAACTAAGCAAGGAATCTGTTCAAGTGTGACGGACGGAAAAGGAATGCTGCCTATGATTCAATGCCTACGTGGACAGGGCAGAGTTCATAGCAAGACAAGAATGACTATTAAAGCATGCCATATTTTGAGTAGTATAAGAAAAGGAAATTATTTATGTCACACAAAGTTCGAGGAAGCTGCTACTAAGACAAAGAGGTCGACAGTGTGTAAGAACAAACAAGCTAATTGCTCATCTAAAAATCAATTCCAGAGTGGGCAAGGGAAAAGTTCTGGCAACACACCGAAAGGAGAAGTGCATTGTTATTTGATAAATCTGACACATTCCCATTCTCAGTCTCTCTCTGAGGCCACCAAAGCCACCTTCCAAAAGAAAACATGTCCTCAGTCAAAGCATCAAGACTCTGATGCTGTATGTGGACTCGTTGTTGAATCAACTGAAGATAACATTAATTTTCTGGTTAATCATCCTCCACTAACAGAGACTATGGATGCTTCAAATGAGAAGCTACCTGGTCAAATTTCATCTCCACCAACAGAAGCAGCTGAGCCTCAGACTCCTCAAGAGCCAAGCATTCAATTCCCATCCCATGCACCTTCATCTCATCCCAGCCCTTCTGGTTCTCATACCATACGTGACCCTGAGGTGTCAAAGCCTACAAATTCTATACGAGCTTTGGAGTTTCTGGAACGTTATCTACACATGCATTCAGATATTGGTATCAAAACTGAAGAAACTCCCATTCCTAAATCTGAAGAAATTTCTCAAGCCAAGGTCAAGATCCAAAGTATTTCAACACTTCCTCTGGACAAAGTCATGGAAACTGAATGTTTGCAAAGCTTCAAATCATCACTGGAAACTTTGCTCAGATCTAATGACCAAACTGCAGTGCAGTTGGCAAACATTGAAGATGTCCTGAGAAAGCTTGCTTTTCTGGAGTCCAAGTACCCCTCAGCTAGTGGAATCACAGCTGAAGTTAACAAAGCATTTGTTCAGAAAGTACAGCTGGAACAGGAAGTGGAAGTCTACACTGGCCAAATTTCTGAAGCTAAAAAAGAAGAGGCAAAGTTTTTCGAGTGTGTAAGTATACAGAAGTCGAGAGTTGAGGAACTTGAGACAAAATTGGCTGAAGAGAAAGCTACTTTGATTGCATTGGAGAAGTACCATGCCACCTACAAAAGCAAAATGGATGGATTGAATTACAAGTGTGAGCAATTGCAGCAGATGCTCTGTCATCATAGTATTAATGAGAGAACTTGGGTGAACAAAGCTTTGCAAGCAAGAGGTGATTTTAAATCGATAGAAGCAACTTGGGTTCAGATCCAGCATCTTGTTTCCACAATTGGTGGCAGCAGCAAGTGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1060

Amino Acids

120.37

Weight (kDa)

8.25

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 116 - 478 1.7e-44 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g26742 FvH4_3g26742
malus_domestica MD11G1175200.v1.1
pyrus_communis pycom11g15030
rosa_chinensis RchiOBHm_Chr5g0049171
rosa_laevigata RLG00000034596
rosa_multiflora Rmu_sc0001677.1_g000029
rosa_roxburghii Rroxscaffold_1G00032560
rosa_rugosa Rorug05G0244500 Rorug05G0244600 Rorug05G0244700 Rorug05G0244800 Rorug05G0244900
rosa_samantha Rh5AG323200 Rh5BG333700 Rh5CG358800 Rh5DG345200
rosa_wichuraiana Rw5G030510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2011
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 1184
AccI GTMKAC 3 cut(s) 2015, 2841, 2902
AclI AACGTT 2 cut(s) 1196, 2506
AclWI GGATC 5 cut(s) 275, 356, 492, 2590, 3130
AcoI YGGCCR 2 cut(s) 27, 2848
AcuI CTGAAG 7 cut(s) 222, 2274, 2565, 2589, 2808, 2880, 2964
AfaI GTAC 9 cut(s) 99, 405, 770, 1408, 1779, 1792, 2762, 2817, 2978
AfiI CCNNNNNNNGG 3 cut(s) 93, 1184, 1678
AflIII ACRYGT 2 cut(s) 1125, 2192
AjnI CCWGG 4 cut(s) 271, 783, 1786, 2326
AjuI GAANNNNNNNTTGG 2 cut(s) 2162, 2194
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
Alw21I GWGCWC 2 cut(s) 496, 1234
Alw26I GTCTC 4 cut(s) 298, 2157, 2291, 2924
Alw44I GTGCAC 1 cut(s) 1230
AlwI GGATC 5 cut(s) 275, 356, 492, 2590, 3130
AlwNI CAGNNNCTG 1 cut(s) 1723
AoxI GGCC 5 cut(s) 27, 269, 1575, 2163, 2848
ApaLI GTGCAC 1 cut(s) 1230
ApeKI GCWGC 8 cut(s) 410, 823, 1864, 1994, 2357, 3034, 3165, 3168
AseI ATTAAT 2 cut(s) 2265, 3060
Asp700I GAANNNNTTC 3 cut(s) 207, 246, 1834
AspLEI GCGC 1 cut(s) 1563
AspS9I GGNCC 6 cut(s) 137, 461, 779, 851, 1434, 1576
AsuC2I CCSGG 1 cut(s) 560
AsuHPI GGTGA 6 cut(s) 773, 1386, 1735, 1757, 3088, 3113
AsuII TTCGAA 1 cut(s) 113
AsuNHI GCTAGC 1 cut(s) 1133
AvaII GGWCC 5 cut(s) 137, 461, 779, 851, 1434
AxyI CCTNAGG 3 cut(s) 133, 775, 2457
BaeGI GKGCMC 2 cut(s) 523, 1234
BalI TGGCCA 2 cut(s) 29, 2850
BanI GGYRCC 1 cut(s) 518
BarI GAAGNNNNNNTAC 4 cut(s) 654, 686, 1317, 1349
Bbv12I GWGCWC 2 cut(s) 496, 1234
BbvCI CCTCAGC 1 cut(s) 2767
BbvI GCAGC 7 cut(s) 397, 810, 1851, 1981, 2369, 3046, 3177
BccI CCATC 6 cut(s) 563, 715, 1534, 1589, 2407, 2999
BceAI ACGGC 1 cut(s) 672
BcgI CGANNNNNNTGC 2 cut(s) 1976, 2010
BciT130I CCWGG 4 cut(s) 273, 785, 1788, 2328
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 1 cut(s) 560
BcoDI GTCTC 4 cut(s) 298, 2157, 2291, 2924
BfaI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 1781, 2772
BfmI CTRYAG 6 cut(s) 843, 1161, 1334, 1353, 1638, 2700
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 2684
BisI GCNGC 8 cut(s) 411, 824, 1865, 1995, 2358, 3035, 3166, 3169
BlpI GCTNAGC 1 cut(s) 2361
BlsI GCNGC 8 cut(s) 412, 825, 1866, 1996, 2359, 3036, 3167, 3170
BmcAI AGTACT 2 cut(s) 405, 1779
Bme1390I CCNGG 5 cut(s) 273, 560, 785, 1788, 2328
Bme18I GGWCC 5 cut(s) 137, 461, 779, 851, 1434
BmgT120I GGNCC 6 cut(s) 137, 461, 779, 851, 1434, 1576
BmiI GGNNCC 5 cut(s) 164, 463, 520, 781, 1578
BmrFI CCNGG 5 cut(s) 273, 560, 785, 1788, 2328
BmrI ACTGGG 1 cut(s) 1752
BmtI GCTAGC 1 cut(s) 1137
BmuI ACTGGG 1 cut(s) 1752
BpmI CTGGAG 3 cut(s) 347, 580, 2771
Bpu10I CCTNAGC 1 cut(s) 2767
Bpu1102I GCTNAGC 1 cut(s) 2361
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 4 cut(s) 1446, 2361, 2565, 2948
BpuMI CCSGG 1 cut(s) 560
Bsa29I ATCGAT 1 cut(s) 3113
BsaAI YACGTR 2 cut(s) 1886, 2450
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 6 cut(s) 33, 783, 1067, 1082, 1786, 2586
BsaXI ACNNNNNCTCC 2 cut(s) 2744, 2774
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 1184, 1678
Bse1I ACTGG 7 cut(s) 364, 597, 1237, 1717, 1758, 2673, 2851
Bse21I CCTNAGG 3 cut(s) 133, 775, 2457
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 4 cut(s) 273, 785, 1788, 2328
BseCI ATCGAT 1 cut(s) 3113
BseDI CCNNGG 6 cut(s) 33, 783, 1067, 1082, 1786, 2586
BseLI CCNNNNNNNGG 3 cut(s) 93, 1184, 1678
BseMI GCAATG 1 cut(s) 283
BseNI ACTGG 7 cut(s) 364, 597, 1237, 1717, 1758, 2673, 2851
BseRI GAGGAG 1 cut(s) 2364
BseSI GKGCMC 2 cut(s) 523, 1234
BseXI GCAGC 7 cut(s) 397, 810, 1851, 1981, 2369, 3046, 3177
BseYI CCCAGC 2 cut(s) 344, 2423
BsgI GTGCAG 2 cut(s) 396, 2726
BshFI GGCC 5 cut(s) 29, 271, 1577, 2165, 2850
BshNI GGYRCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 3113
BsiHKAI GWGCWC 2 cut(s) 496, 1234
BsiSI CCGG 1 cut(s) 560
BsiWI CGTACG 1 cut(s) 768
BslFI GGGAC 2 cut(s) 447, 516
BslI CCNNNNNNNGG 3 cut(s) 93, 1184, 1678
BsmAI GTCTC 4 cut(s) 298, 2157, 2291, 2924
BsmFI GGGAC 2 cut(s) 447, 516
BsmI GAATGC 6 cut(s) 826, 1257, 1552, 1866, 2388, 2521
BsnI GGCC 5 cut(s) 29, 271, 1577, 2165, 2850
Bso31I GGTCTC 1 cut(s) 298
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 496, 523, 1234
Bsp143I GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
Bsp1720I GCTNAGC 1 cut(s) 2361
Bsp19I CCATGG 2 cut(s) 33, 1067
BspANI GGCC 5 cut(s) 29, 271, 1577, 2165, 2850
BspDI ATCGAT 1 cut(s) 3113
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 5 cut(s) 164, 463, 520, 781, 1578
BspMAI CTGCAG 1 cut(s) 2704
BspOI GCTAGC 1 cut(s) 1137
BspPI GGATC 5 cut(s) 275, 356, 492, 2590, 3130
BspQI GCTCTTC 2 cut(s) 648, 1179
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 7 cut(s) 364, 597, 1237, 1717, 1758, 2673, 2851
BssECI CCNNGG 6 cut(s) 33, 783, 1067, 1082, 1786, 2586
BssMI GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
BssNAI GTATAC 1 cut(s) 2903
BssT1I CCWWGG 4 cut(s) 33, 1067, 1082, 2586
Bst1107I GTATAC 1 cut(s) 2903
Bst2UI CCWGG 4 cut(s) 273, 785, 1788, 2328
Bst4CI ACNGT 1 cut(s) 2020
Bst6I CTCTTC 5 cut(s) 648, 1014, 1179, 2868, 2940
BstAPI GCANNNNNTGC 1 cut(s) 3040
BstBAI YACGTR 2 cut(s) 1886, 2450
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 5 cut(s) 1135, 1234, 1933, 2742, 3093
BstDSI CCRYGG 2 cut(s) 33, 1067
BstHHI GCGC 1 cut(s) 1563
BstKTI GATC 9 cut(s) 223, 283, 351, 487, 720, 1156, 2598, 2687, 3138
BstMAI GTCTC 4 cut(s) 298, 2157, 2291, 2924
BstMBI GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
BstMWI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2171, 3034, 3040, 3092
BstNI CCWGG 4 cut(s) 273, 785, 1788, 2328
BstNSI RCATGY 5 cut(s) 662, 1129, 1935, 2196, 2521
BstSCI CCNGG 5 cut(s) 271, 558, 783, 1786, 2326
BstSFI CTRYAG 6 cut(s) 843, 1161, 1334, 1353, 1638, 2700
BstSLI GKGCMC 2 cut(s) 523, 1234
BstV1I GCAGC 7 cut(s) 397, 810, 1851, 1981, 2369, 3046, 3177
BstX2I RGATCY 5 cut(s) 348, 484, 2595, 2684, 3135
BstYI RGATCY 5 cut(s) 348, 484, 2595, 2684, 3135
BstZ17I GTATAC 1 cut(s) 2903
Bsu15I ATCGAT 1 cut(s) 3113
Bsu36I CCTNAGG 3 cut(s) 133, 775, 2457
BsuRI GGCC 5 cut(s) 29, 271, 1577, 2165, 2850
BsuTUI ATCGAT 1 cut(s) 3113
BtgI CCRYGG 2 cut(s) 33, 1067
BtsI GCAGTG 2 cut(s) 75, 2709
BtsIMutI CAGTG 6 cut(s) 75, 1724, 2025, 2666, 2709, 2844
Cac8I GCNNGC 5 cut(s) 1135, 1234, 1933, 2742, 3093
CaiI CAGNNNCTG 1 cut(s) 1723
CciI TCATGA 1 cut(s) 982
CfoI GCGC 1 cut(s) 1563
Cfr13I GGNCC 6 cut(s) 137, 461, 779, 851, 1434, 1576
ClaI ATCGAT 1 cut(s) 3113
CseI GACGC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 2326
Csp6I GTAC 9 cut(s) 98, 404, 769, 1407, 1778, 1791, 2761, 2816, 2977
CviQI GTAC 9 cut(s) 98, 404, 769, 1407, 1778, 1791, 2761, 2816, 2977
DpnI GATC 9 cut(s) 222, 282, 350, 486, 719, 1155, 2597, 2686, 3137
DpnII GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
DraI TTTAAA 2 cut(s) 304, 3109
DrdI GACNNNNNNGTC 1 cut(s) 2011
DseDI GACNNNNNNGTC 1 cut(s) 2011
EaeI YGGCCR 2 cut(s) 27, 2848
Eam1104I CTCTTC 5 cut(s) 648, 1014, 1179, 2868, 2940
EarI CTCTTC 5 cut(s) 648, 1014, 1179, 2868, 2940
Eco130I CCWWGG 4 cut(s) 33, 1067, 1082, 2586
Eco31I GGTCTC 1 cut(s) 298
Eco47I GGWCC 5 cut(s) 137, 461, 779, 851, 1434
Eco57I CTGAAG 7 cut(s) 222, 2274, 2565, 2589, 2808, 2880, 2964
Eco81I CCTNAGG 3 cut(s) 133, 775, 2457
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 4 cut(s) 271, 783, 1786, 2326
EcoT14I CCWWGG 4 cut(s) 33, 1067, 1082, 2586
EcoT22I ATGCAT 3 cut(s) 25, 886, 2523
ErhI CCWWGG 4 cut(s) 33, 1067, 1082, 2586
FalI AAGNNNNNCTT 4 cut(s) 195, 227, 1439, 1471
FaqI GGGAC 2 cut(s) 447, 516
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 3 cut(s) 2015, 2841, 2902
Fnu4HI GCNGC 8 cut(s) 411, 824, 1865, 1995, 2358, 3035, 3166, 3169
FokI GGATG 9 cut(s) 89, 542, 578, 1402, 2268, 2318, 2386, 2406, 3017
Fsp4HI GCNGC 8 cut(s) 411, 824, 1865, 1995, 2358, 3035, 3166, 3169
FspBI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 1781, 2772
GlaI GCGC 1 cut(s) 1562
GluI GCNGC 8 cut(s) 411, 824, 1865, 1995, 2358, 3035, 3166, 3169
GsaI CCCAGC 2 cut(s) 348, 2427
GsuI CTGGAG 3 cut(s) 347, 580, 2771
HaeIII GGCC 5 cut(s) 29, 271, 1577, 2165, 2850
HapII CCGG 1 cut(s) 560
HgaI GACGC 1 cut(s) 890
HhaI GCGC 1 cut(s) 1563
Hin6I GCGC 1 cut(s) 1561
HinP1I GCGC 1 cut(s) 1561
HincII GTYRAC 3 cut(s) 1669, 2016, 2794
HindII GTYRAC 3 cut(s) 1669, 2016, 2794
HindIII AAGCTT 5 cut(s) 1425, 1484, 2653, 2738, 3084
HpaI GTTAAC 1 cut(s) 2794
HpaII CCGG 1 cut(s) 560
HphI GGTGA 6 cut(s) 773, 1386, 1735, 1757, 3088, 3113
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 8 cut(s) 615, 629, 704, 1429, 1698, 2188, 2421, 2439
HpyCH4III ACNGT 1 cut(s) 2020
HpyCH4IV ACGT 4 cut(s) 1196, 1885, 2449, 2506
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2171, 3034, 3040, 3092
HpySE526I ACGT 4 cut(s) 1196, 1885, 2449, 2506
HspAI GCGC 1 cut(s) 1561
Ksp22I TGATCA 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 2794
Kzo9I GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
LguI GCTCTTC 2 cut(s) 648, 1179
LmnI GCTCC 1 cut(s) 638
Lsp1109I GCAGC 7 cut(s) 397, 810, 1851, 1981, 2369, 3046, 3177
MabI ACCWGGT 1 cut(s) 2326
MaeI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 1781, 2772
MaeII ACGT 4 cut(s) 1196, 1885, 2449, 2506
MaeIII GTNAC 7 cut(s) 643, 689, 1348, 1816, 1843, 1973, 2450
MalI GATC 9 cut(s) 222, 282, 350, 486, 719, 1155, 2597, 2686, 3137
MboI GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
MfeI CAATTG 2 cut(s) 3029, 3156
MflI RGATCY 5 cut(s) 348, 484, 2595, 2684, 3135
MhlI GDGCHC 3 cut(s) 496, 523, 1234
MlsI TGGCCA 2 cut(s) 29, 2850
MluNI TGGCCA 2 cut(s) 29, 2850
MlyI GAGTC 5 cut(s) 773, 2210, 2229, 2365, 2762
MmeI TCCRAC 1 cut(s) 1690
Mox20I TGGCCA 2 cut(s) 29, 2850
Mph1103I ATGCAT 3 cut(s) 25, 886, 2523
MroXI GAANNNNTTC 3 cut(s) 207, 246, 1834
MscI TGGCCA 2 cut(s) 29, 2850
Msp20I TGGCCA 2 cut(s) 29, 2850
MspA1I CMGCKG 3 cut(s) 2360, 2786, 2821
MspI CCGG 1 cut(s) 560
MspR9I CCNGG 5 cut(s) 273, 560, 785, 1788, 2328
MunI CAATTG 2 cut(s) 3029, 3156
Mva1269I GAATGC 6 cut(s) 826, 1257, 1552, 1866, 2388, 2521
MvaI CCWGG 4 cut(s) 273, 785, 1788, 2328
MwoI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2171, 3034, 3040, 3092
NciI CCSGG 1 cut(s) 560
NcoI CCATGG 2 cut(s) 33, 1067
NdeII GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
NheI GCTAGC 1 cut(s) 1133
NlaIV GGNNCC 5 cut(s) 164, 463, 520, 781, 1578
NmuCI GTSAC 4 cut(s) 643, 1843, 1973, 2450
NsiI ATGCAT 3 cut(s) 25, 886, 2523
NspI RCATGY 5 cut(s) 662, 1129, 1935, 2196, 2521
NspV TTCGAA 1 cut(s) 113
PaeI GCATGC 1 cut(s) 1935
PagI TCATGA 1 cut(s) 982
PciI ACATGT 2 cut(s) 1125, 2192
PciSI GCTCTTC 2 cut(s) 648, 1179
PctI GAATGC 6 cut(s) 826, 1257, 1552, 1866, 2388, 2521
PdmI GAANNNNTTC 3 cut(s) 207, 246, 1834
PfeI GAWTC 9 cut(s) 52, 545, 862, 1249, 1713, 1830, 1873, 2246, 2778
Pfl23II CGTACG 1 cut(s) 768
PflFI GACNNNGTC 1 cut(s) 2627
PflMI CCANNNNNTGG 1 cut(s) 1184
PfoI TCCNGGA 1 cut(s) 558
PkrI GCNGC 8 cut(s) 412, 825, 1866, 1996, 2359, 3036, 3167, 3170
PleI GAGTC 5 cut(s) 772, 2210, 2229, 2365, 2761
PpsI GAGTC 5 cut(s) 772, 2210, 2229, 2365, 2761
Ppu21I YACGTR 2 cut(s) 1886, 2450
PpuMI RGGWCCY 1 cut(s) 779
PscI ACATGT 2 cut(s) 1125, 2192
PshBI ATTAAT 2 cut(s) 2265, 3060
Psp1406I AACGTT 2 cut(s) 1196, 2506
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 4 cut(s) 271, 783, 1786, 2326
PspFI CCCAGC 2 cut(s) 344, 2423
PspGI CCWGG 4 cut(s) 271, 783, 1786, 2326
PspLI CGTACG 1 cut(s) 768
PspN4I GGNNCC 5 cut(s) 164, 463, 520, 781, 1578
PspPI GGNCC 6 cut(s) 137, 461, 779, 851, 1434, 1576
PspPPI RGGWCCY 1 cut(s) 779
PsrI GAACNNNNNNTAC 2 cut(s) 2496, 2528
PstI CTGCAG 1 cut(s) 2704
PstNI CAGNNNCTG 1 cut(s) 1723
PsuI RGATCY 5 cut(s) 348, 484, 2595, 2684, 3135
PsyI GACNNNGTC 1 cut(s) 2627
PvuII CAGCTG 3 cut(s) 2360, 2786, 2821
RsaI GTAC 9 cut(s) 99, 405, 770, 1408, 1779, 1792, 2762, 2817, 2978
RsaNI GTAC 9 cut(s) 98, 404, 769, 1407, 1778, 1791, 2761, 2816, 2977
SalI GTCGAC 1 cut(s) 2014
SapI GCTCTTC 2 cut(s) 648, 1179
SatI GCNGC 8 cut(s) 411, 824, 1865, 1995, 2358, 3035, 3166, 3169
Sau3AI GATC 9 cut(s) 220, 280, 348, 484, 717, 1153, 2595, 2684, 3135
Sau96I GGNCC 6 cut(s) 137, 461, 779, 851, 1434, 1576
ScaI AGTACT 2 cut(s) 405, 1779
SchI GAGTC 5 cut(s) 773, 2210, 2229, 2365, 2762
ScrFI CCNGG 5 cut(s) 273, 560, 785, 1788, 2328
SduI GDGCHC 3 cut(s) 496, 523, 1234
SexAI ACCWGGT 1 cut(s) 2326
SfcI CTRYAG 6 cut(s) 843, 1161, 1334, 1353, 1638, 2700
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 5 cut(s) 137, 461, 779, 851, 1434
SmlI CTYRAG 4 cut(s) 1461, 2376, 2580, 2927
SmoI CTYRAG 4 cut(s) 1461, 2376, 2580, 2927
SphI GCATGC 1 cut(s) 1935
SspI AATATT 2 cut(s) 631, 1077
SspMI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 1781, 2772
StyD4I CCNGG 5 cut(s) 271, 558, 783, 1786, 2326
StyI CCWWGG 4 cut(s) 33, 1067, 1082, 2586
TaaI ACNGT 1 cut(s) 2020
TaiI ACGT 4 cut(s) 1199, 1888, 2452, 2509
TaqI TCGA 6 cut(s) 113, 1986, 2015, 2889, 2912, 3113
TatI WGTACW 3 cut(s) 403, 1777, 2815
TfiI GAWTC 9 cut(s) 52, 545, 862, 1249, 1713, 1830, 1873, 2246, 2778
TscAI CASTG 6 cut(s) 82, 1724, 2025, 2673, 2709, 2851
TseFI GTSAC 4 cut(s) 643, 1843, 1973, 2450
TseI GCWGC 8 cut(s) 410, 823, 1864, 1994, 2357, 3034, 3165, 3168
Tsp45I GTSAC 4 cut(s) 643, 1843, 1973, 2450
TspGWI ACGGA 3 cut(s) 1112, 1862, 1866
TspRI CASTG 6 cut(s) 82, 1724, 2025, 2673, 2709, 2851
Tth111I GACNNNGTC 1 cut(s) 2627
Van91I CCANNNNNTGG 1 cut(s) 1184
VneI GTGCAC 1 cut(s) 1230
VpaK11BI GGWCC 5 cut(s) 137, 461, 779, 851, 1434
VspI ATTAAT 2 cut(s) 2265, 3060
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 5 cut(s) 662, 1129, 1935, 2196, 2521
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmiI GTMKAC 3 cut(s) 2015, 2841, 2902
XmnI GAANNNNTTC 3 cut(s) 207, 246, 1834
XspI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 1781, 2772
ZrmI AGTACT 2 cut(s) 405, 1779
Zsp2I ATGCAT 3 cut(s) 25, 886, 2523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.