Rw5G030510

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013644
Physical Location & Seq
Forward (+)
48491184 .. 48494682
3499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030510.1

Sequence Viewer

Length: 3015 bp
ATGGCATCCTCTAGAGATGATGCATTGGCCACCATGGCTGTCAATAGAGTGAATCAACTTATGGAGGAAATAGGCACTGCAACCACCGATTTTCGGGTACTCATCCCTCATTCGAATTCTCAGCACTTTGTCTTAGGTCCAGCACTCAAATCTGACAATAGCTTCCCTGACATTTATAGTGATTTTCCCAAAACGAAAACTGAAGATTTTCTTATACATGATCAAAATGCCAAGTTCTATGAATGGTTTCATCAGAAGCATCGGTTTTGGCCTGGCAATGATCCTGATTGGGTCTCTTGGTTTAAAAGAGTTGAAGCTCAAAAGAGTTACGTTTGGCATCAAGCTGGGATCTATGACATTCTCCAGTTCTCTAAGTTTACCATTCCTCTTAATCGTTCTTTAGTACTAGCTGCACTTTGCTTTTGGTCTATTTCCACAAATACTTTTCATTTTGGTCATGGTCCCCTTACTATTACTCTCTTGGACCTTAGTGCTCTGGTAGGACTTAGACCATATGGTGCCCAATTTGATGCTGTCATTGCTGATTCCATATCCATCCCAGAGGATAATTATGGCATTGAAGTTAATCAGTTCTCCAGTTTTCCTTCATTTATTAGGGAACATAACAATATTGAAGGAGCTGTGACAGATGAAGAGCATGTAGCATTTCTTCACTATTGGCTTTGCCGTTACCTTCTTTGTTCTCCATCATTCAAGATCATCAAAACCAATTTGCCTTTGGCTGTGGCTTTGTCTAGGGGTGAGTCGTACGCCTTAGGACCCCTGGTTCTTGCCTACTTGTATAGAGGCATCAAGGACTTGCTGCACTCTCAGCTAAATACTACAGGTGGACCAATATGGATTCTTCAACTTTGGCTTCATGCATACTTTCCCAAAATGCGTCCAGATTGTTTTGCAATTCCCAACCTCTCCTGTCATGGACTAAAATATGTTTCTTTCAAACAGCCTAAACTTTTGTTTCATGATTGTTTATTGTATATGAGCTACTTAGACAGAGAAGAGAATTTCAACTTCAATCCATATTCAAAGTTAGATGCTTTACCTCCATGGTTAATATTTCCTTGGACTGAAATAACGGAAACTGAATTTTCTACACTCAAGGACATGTGGGCTAGCTTTCTTGTTAGTCGTGATCTTCCTGTAGGTATAGCTCTTCCAAAATTTGGAAACAAAACGTTTGGTTTTGAAGCATATTGCCCAAATCTTTGTGCACGCCAGTTCGGGCTGATTCAAGGCATTCCTACTCCCCCTTTCTTCTCTCTTAATACGCATTTCCCAGAAAAGATGGTTGTTCGCAGTTTTGAAGAACTACTCCAGGTGCTTAAAGTTACTACAGCTCATTTGTTCACAAAACTTGAACTTTCTTCATATTCACCACTACCTAGTACCACCTCATCCTTCAAAGCTTGGTGGACCAAATACTACAAGCACACCTTTCCTCAAGATTTGAGCTATCTTGAAAAGCTTGATGACTTGACGCGAAACAGAACGCCTCGCCAAGAGCCATCAGAGAAAGCAAGAAAGAATGCTTTGAGGAAGCGCAAAGGAGGGTTAATCACCATTCAAGACCCCAGTTCTCACCAACAGCGAGTACCAGACCCTGGTACATCAAGAAAAAGGATTAGGTATGTAACTAAGCAAGGAATCTGTTCAAGTGTGACAGACGTAAAAGGAAAGCTGCCTATGATTCAATGCCTACGTGGACAGGGCAGAGTTCATAGCAAGACAAGAATGACTATTAAAGCATGCCATATTTTGAGAAGTATAAGAAAGGGAAATTATTTATGTCACACCAAGTTCGAGGAAGCTGCTACTAAGACAAAGAGGTCGACAGTGTGTAAGAACAAACAAGCTAATTGCTCATCTAAAAATCAATTCCAGAGTGGCCAAGGGAAAAGTTCTGGCAACACACCGAAAGGAGCAGTGCATTGTTATTTGATAAATCTGACACATTCCCATTCTCAGTCTCTCTCTGAGGCCACCAAAGCCACCTTCCAAAAGAAAACATGTCCTCAGTCAAAGGATCAAGACTCTGATGCTGTATGTGGACTCGTTGTTGAATCAACTGAAGATAACATTAATTTTCAGGTTAATGATCCTCCACTAACAGAGACTATGGATGCTTCAAATGAGAAGCTACCTGGTCAAATTTCACCTCCGCCAACAGAAGCAGCTGAGCCTCAGACTCCTCAAGAGCCAAGCATTCAATTCCCATTCCATGCACCTTCATCTCATCCCAACCCTTCTAGTTCTCATACCATACGTGACCCTGAGGTGTCAAATCCTACAAATTCTATACGAGCTTTGGAGTTTCTGGAACGTTATCTACACATGCATTCAGATATTGGTATCAAAACTGAAGAAACTCCCATTCCTAAATCTGAAGAAACTTCTCAAGCCAAGGTCAAGATCCAAAGTATTGCAACACTTCCTCTGGATAAAGTCATGGAAACTGAATGTTTGCAAAGCTTCAAATCATCACTGGAAACTTTGCTCAGATCTAATGACCAAACTGCAGTGCAGTTGGCAAACATTGAAGATGTCCTGAGAAAGCTTGCTTTTCTGGAGTCCAAGTACCCCTCAGCTAGTGGAATCACAGCTGAAGTTAACAAAGCATTTGTTCAGAAAGTACAGCTGGAACAGGAAGTGGAAGTCTACACTGGCCAAATTTCTGAAGCTAAAAAAGAAGAGGCAAAGTTTTTCGAGTGTGTAAGTATACAGAAGTCGAGAGTTGAGGAACTTGAGACAAAATTGGCTGAAGAGAAAGCTACTTTAATTGCATTGGAGAAGTACCATGCCACCTACAAAATCAAAATGGATGAATTGAATTACAAGTGTGAGCAAGTGCAGCAGATGCTCTGTCATCATAGTATTAATGAGAGAACTTTGGTGAACAAAGCTTTGCAAGCAAGAGGTGATTTTAAATCGATGGAAGCAACTTGGGTTCAGATCCGGCATCTTGTTTCCTCAATTGGTGGCAGCAAGCGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1004

Amino Acids

114.01

Weight (kDa)

8.43

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 116 - 426 1.1e-44 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g26742 FvH4_3g26742
malus_domestica MD11G1175200.v1.1
pyrus_communis pycom11g15030
rosa_chinensis RchiOBHm_Chr5g0049171
rosa_laevigata RLG00000034596
rosa_multiflora Rmu_sc0001677.1_g000029
rosa_roxburghii Rroxscaffold_1G00032560
rosa_rugosa Rorug05G0244500 Rorug05G0244600 Rorug05G0244700 Rorug05G0244800 Rorug05G0244900
rosa_samantha Rh5AG323200 Rh5BG333700 Rh5CG358800 Rh5DG345200
rosa_wichuraiana Rw5G030510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1846
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 2 cut(s) 1184, 1622
AccI GTMKAC 3 cut(s) 1850, 2676, 2737
AccII CGCG 1 cut(s) 1501
AciI CCGC 2 cut(s) 2180, 3007
AclI AACGTT 2 cut(s) 1196, 2341
AclWI GGATC 6 cut(s) 275, 356, 2052, 2111, 2425, 2965
AcoI YGGCCR 3 cut(s) 27, 1906, 2683
AcsI RAATTY 7 cut(s) 115, 1024, 1106, 1181, 2169, 2311, 2688
AcuI CTGAAG 7 cut(s) 222, 2109, 2400, 2424, 2643, 2715, 2799
AfaI GTAC 9 cut(s) 99, 405, 770, 1408, 1614, 1627, 2597, 2652, 2813
AfiI CCNNNNNNNGG 3 cut(s) 93, 1184, 1622
AflIII ACRYGT 2 cut(s) 1125, 2027
AjnI CCWGG 5 cut(s) 271, 783, 1335, 1621, 2161
AjuI GAANNNNNNNTTGG 2 cut(s) 1997, 2029
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
Alw21I GWGCWC 2 cut(s) 496, 1234
Alw26I GTCTC 4 cut(s) 298, 1992, 2126, 2759
Alw44I GTGCAC 1 cut(s) 1230
AlwI GGATC 6 cut(s) 275, 356, 2052, 2111, 2425, 2965
AlwNI CAGNNNCTG 1 cut(s) 1622
AoxI GGCC 5 cut(s) 27, 269, 1906, 1998, 2683
ApaLI GTGCAC 1 cut(s) 1230
ApeKI GCWGC 7 cut(s) 410, 823, 1699, 1829, 2192, 2869, 3000
ApoI RAATTY 7 cut(s) 115, 1024, 1106, 1181, 2169, 2311, 2688
AseI ATTAAT 2 cut(s) 2100, 2895
Asp700I GAANNNNTTC 3 cut(s) 207, 246, 1669
AspLEI GCGC 1 cut(s) 1563
AspS9I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
AsuHPI GGTGA 7 cut(s) 773, 1386, 1570, 1592, 2166, 2923, 2948
AsuII TTCGAA 1 cut(s) 113
AsuNHI GCTAGC 1 cut(s) 1133
AvaII GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
AxyI CCTNAGG 2 cut(s) 775, 2292
BaeGI GKGCMC 2 cut(s) 523, 1234
BalI TGGCCA 3 cut(s) 29, 1908, 2685
BanI GGYRCC 1 cut(s) 518
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
Bbv12I GWGCWC 2 cut(s) 496, 1234
BbvCI CCTCAGC 1 cut(s) 2602
BbvI GCAGC 6 cut(s) 397, 810, 1686, 1816, 2204, 2881
BccI CCATC 5 cut(s) 563, 715, 1300, 1534, 2944
BceAI ACGGC 1 cut(s) 672
BcgI CGANNNNNNTGC 2 cut(s) 1811, 1845
BciT130I CCWGG 5 cut(s) 273, 785, 1337, 1623, 2163
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 4 cut(s) 298, 1992, 2126, 2759
BfaI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2268, 2607
BfmI CTRYAG 4 cut(s) 843, 1161, 1353, 2535
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 2519
BisI GCNGC 7 cut(s) 411, 824, 1700, 1830, 2193, 2870, 3001
BlpI GCTNAGC 1 cut(s) 2196
BlsI GCNGC 7 cut(s) 412, 825, 1701, 1831, 2194, 2871, 3002
BmcAI AGTACT 1 cut(s) 405
Bme1390I CCNGG 5 cut(s) 273, 785, 1337, 1623, 2163
Bme18I GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
BmgT120I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
BmiI GGNNCC 3 cut(s) 463, 520, 781
BmrFI CCNGG 5 cut(s) 273, 785, 1337, 1623, 2163
BmrI ACTGGG 1 cut(s) 1587
BmtI GCTAGC 1 cut(s) 1137
BmuI ACTGGG 1 cut(s) 1587
BpmI CTGGAG 4 cut(s) 347, 580, 1319, 2606
Bpu10I CCTNAGC 1 cut(s) 2602
Bpu1102I GCTNAGC 1 cut(s) 2196
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 5 cut(s) 1103, 1446, 2196, 2400, 2783
Bsa29I ATCGAT 1 cut(s) 2948
BsaAI YACGTR 2 cut(s) 1721, 2285
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 7 cut(s) 33, 783, 1067, 1082, 1621, 1909, 2421
BsaXI ACNNNNNCTCC 2 cut(s) 2579, 2609
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 1184, 1622
Bse1I ACTGG 6 cut(s) 364, 597, 1237, 1593, 2508, 2686
Bse21I CCTNAGG 2 cut(s) 775, 2292
Bse3DI GCAATG 2 cut(s) 283, 537
BseBI CCWGG 5 cut(s) 273, 785, 1337, 1623, 2163
BseCI ATCGAT 1 cut(s) 2948
BseDI CCNNGG 7 cut(s) 33, 783, 1067, 1082, 1621, 1909, 2421
BseGI GGATG 7 cut(s) 5, 102, 555, 1415, 2146, 2254, 2845
BseLI CCNNNNNNNGG 3 cut(s) 93, 1184, 1622
BseMI GCAATG 2 cut(s) 283, 537
BseNI ACTGG 6 cut(s) 364, 597, 1237, 1593, 2508, 2686
BseRI GAGGAG 1 cut(s) 2199
BseSI GKGCMC 2 cut(s) 523, 1234
BseXI GCAGC 6 cut(s) 397, 810, 1686, 1816, 2204, 2881
BseYI CCCAGC 1 cut(s) 344
BsgI GTGCAG 4 cut(s) 396, 809, 2561, 2888
Bsh1236I CGCG 1 cut(s) 1501
BshFI GGCC 5 cut(s) 29, 271, 1908, 2000, 2685
BshNI GGYRCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 2948
BsiHKAI GWGCWC 2 cut(s) 496, 1234
BsiSI CCGG 1 cut(s) 2974
BsiWI CGTACG 1 cut(s) 768
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 3 cut(s) 93, 1184, 1622
BsmAI GTCTC 4 cut(s) 298, 1992, 2126, 2759
BsmFI GGGAC 1 cut(s) 447
BsmI GAATGC 4 cut(s) 1257, 1552, 2223, 2356
BsnI GGCC 5 cut(s) 29, 271, 1908, 2000, 2685
Bso31I GGTCTC 1 cut(s) 298
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 496, 523, 1234
Bsp1720I GCTNAGC 1 cut(s) 2196
Bsp19I CCATGG 2 cut(s) 33, 1067
BspACI CCGC 2 cut(s) 2180, 3007
BspANI GGCC 5 cut(s) 29, 271, 1908, 2000, 2685
BspDI ATCGAT 1 cut(s) 2948
BspFNI CGCG 1 cut(s) 1501
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 3 cut(s) 463, 520, 781
BspMAI CTGCAG 1 cut(s) 2539
BspOI GCTAGC 1 cut(s) 1137
BspPI GGATC 6 cut(s) 275, 356, 2052, 2111, 2425, 2965
BspQI GCTCTTC 2 cut(s) 648, 1179
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 283, 537
BsrI ACTGG 6 cut(s) 364, 597, 1237, 1593, 2508, 2686
BssECI CCNNGG 7 cut(s) 33, 783, 1067, 1082, 1621, 1909, 2421
BssNAI GTATAC 1 cut(s) 2738
BssT1I CCWWGG 5 cut(s) 33, 1067, 1082, 1909, 2421
Bst1107I GTATAC 1 cut(s) 2738
Bst2UI CCWGG 5 cut(s) 273, 785, 1337, 1623, 2163
Bst4CI ACNGT 1 cut(s) 1855
Bst6I CTCTTC 5 cut(s) 648, 1014, 1179, 2703, 2775
BstAPI GCANNNNNTGC 1 cut(s) 2875
BstBAI YACGTR 2 cut(s) 1721, 2285
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 6 cut(s) 1135, 1234, 1768, 2577, 2928, 3005
BstDSI CCRYGG 2 cut(s) 33, 1067
BstF5I GGATG 7 cut(s) 5, 102, 555, 1415, 2146, 2254, 2845
BstFNI CGCG 1 cut(s) 1501
BstHHI GCGC 1 cut(s) 1563
BstMAI GTCTC 4 cut(s) 298, 1992, 2126, 2759
BstMWI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2006, 2869, 2875, 2927
BstNI CCWGG 5 cut(s) 273, 785, 1337, 1623, 2163
BstNSI RCATGY 5 cut(s) 662, 1129, 1770, 2031, 2356
BstSCI CCNGG 5 cut(s) 271, 783, 1335, 1621, 2161
BstSFI CTRYAG 4 cut(s) 843, 1161, 1353, 2535
BstSLI GKGCMC 2 cut(s) 523, 1234
BstUI CGCG 1 cut(s) 1501
BstV1I GCAGC 6 cut(s) 397, 810, 1686, 1816, 2204, 2881
BstX2I RGATCY 4 cut(s) 348, 2430, 2519, 2970
BstYI RGATCY 4 cut(s) 348, 2430, 2519, 2970
BstZ17I GTATAC 1 cut(s) 2738
Bsu15I ATCGAT 1 cut(s) 2948
Bsu36I CCTNAGG 2 cut(s) 775, 2292
BsuRI GGCC 5 cut(s) 29, 271, 1908, 2000, 2685
BsuTUI ATCGAT 1 cut(s) 2948
BtgI CCRYGG 2 cut(s) 33, 1067
BtsCI GGATG 7 cut(s) 5, 102, 555, 1415, 2146, 2254, 2845
BtsI GCAGTG 3 cut(s) 75, 1950, 2544
BtsIMutI CAGTG 6 cut(s) 75, 1860, 1950, 2501, 2544, 2679
Cac8I GCNNGC 6 cut(s) 1135, 1234, 1768, 2577, 2928, 3005
CaiI CAGNNNCTG 1 cut(s) 1622
CciI TCATGA 1 cut(s) 982
CfoI GCGC 1 cut(s) 1563
Cfr13I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
ClaI ATCGAT 1 cut(s) 2948
CseI GACGC 2 cut(s) 890, 1507
CsiI ACCWGGT 1 cut(s) 2161
Csp6I GTAC 9 cut(s) 98, 404, 769, 1407, 1613, 1626, 2596, 2651, 2812
CviQI GTAC 9 cut(s) 98, 404, 769, 1407, 1613, 1626, 2596, 2651, 2812
DraI TTTAAA 2 cut(s) 304, 2944
DrdI GACNNNNNNGTC 1 cut(s) 1846
DseDI GACNNNNNNGTC 1 cut(s) 1846
EaeI YGGCCR 3 cut(s) 27, 1906, 2683
Eam1104I CTCTTC 5 cut(s) 648, 1014, 1179, 2703, 2775
EarI CTCTTC 5 cut(s) 648, 1014, 1179, 2703, 2775
EciI GGCGGA 1 cut(s) 2169
Eco130I CCWWGG 5 cut(s) 33, 1067, 1082, 1909, 2421
Eco31I GGTCTC 1 cut(s) 298
Eco47I GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
Eco57I CTGAAG 7 cut(s) 222, 2109, 2400, 2424, 2643, 2715, 2799
Eco81I CCTNAGG 2 cut(s) 775, 2292
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 5 cut(s) 271, 783, 1335, 1621, 2161
EcoT14I CCWWGG 5 cut(s) 33, 1067, 1082, 1909, 2421
EcoT22I ATGCAT 3 cut(s) 25, 886, 2358
ErhI CCWWGG 5 cut(s) 33, 1067, 1082, 1909, 2421
FalI AAGNNNNNCTT 4 cut(s) 195, 227, 1439, 1471
FaqI GGGAC 1 cut(s) 447
FauNDI CATATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 3 cut(s) 1850, 2676, 2737
Fnu4HI GCNGC 7 cut(s) 411, 824, 1700, 1830, 2193, 2870, 3001
FokI GGATG 6 cut(s) 89, 542, 1402, 2153, 2241, 2852
Fsp4HI GCNGC 7 cut(s) 411, 824, 1700, 1830, 2193, 2870, 3001
FspBI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2268, 2607
GlaI GCGC 1 cut(s) 1562
GluI GCNGC 7 cut(s) 411, 824, 1700, 1830, 2193, 2870, 3001
GsaI CCCAGC 1 cut(s) 348
GsuI CTGGAG 4 cut(s) 347, 580, 1319, 2606
HaeIII GGCC 5 cut(s) 29, 271, 1908, 2000, 2685
HapII CCGG 1 cut(s) 2974
HgaI GACGC 2 cut(s) 890, 1507
HhaI GCGC 1 cut(s) 1563
Hin6I GCGC 1 cut(s) 1561
HinP1I GCGC 1 cut(s) 1561
HincII GTYRAC 2 cut(s) 1851, 2629
HindII GTYRAC 2 cut(s) 1851, 2629
HindIII AAGCTT 5 cut(s) 1425, 1484, 2488, 2573, 2919
HpaI GTTAAC 1 cut(s) 2629
HpaII CCGG 1 cut(s) 2974
HphI GGTGA 7 cut(s) 773, 1386, 1570, 1592, 2166, 2923, 2948
HpyAV CCTTC 7 cut(s) 615, 629, 704, 1429, 2023, 2256, 2274
HpyCH4III ACNGT 1 cut(s) 1855
HpyCH4IV ACGT 6 cut(s) 330, 1196, 1686, 1720, 2284, 2341
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2006, 2869, 2875, 2927
HpySE526I ACGT 6 cut(s) 330, 1196, 1686, 1720, 2284, 2341
HspAI GCGC 1 cut(s) 1561
Ksp22I TGATCA 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 2629
LguI GCTCTTC 2 cut(s) 648, 1179
LmnI GCTCC 2 cut(s) 638, 1940
Lsp1109I GCAGC 6 cut(s) 397, 810, 1686, 1816, 2204, 2881
MabI ACCWGGT 1 cut(s) 2161
MaeI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2268, 2607
MaeII ACGT 6 cut(s) 330, 1196, 1686, 1720, 2284, 2341
MaeIII GTNAC 8 cut(s) 326, 643, 689, 1348, 1651, 1678, 1808, 2285
MfeI CAATTG 1 cut(s) 2991
MflI RGATCY 4 cut(s) 348, 2430, 2519, 2970
MhlI GDGCHC 3 cut(s) 496, 523, 1234
MlsI TGGCCA 3 cut(s) 29, 1908, 2685
MluNI TGGCCA 3 cut(s) 29, 1908, 2685
MlyI GAGTC 5 cut(s) 773, 2045, 2064, 2200, 2597
Mox20I TGGCCA 3 cut(s) 29, 1908, 2685
Mph1103I ATGCAT 3 cut(s) 25, 886, 2358
MroXI GAANNNNTTC 3 cut(s) 207, 246, 1669
MscI TGGCCA 3 cut(s) 29, 1908, 2685
Msp20I TGGCCA 3 cut(s) 29, 1908, 2685
MspA1I CMGCKG 3 cut(s) 2195, 2621, 2656
MspI CCGG 1 cut(s) 2974
MspR9I CCNGG 5 cut(s) 273, 785, 1337, 1623, 2163
MunI CAATTG 1 cut(s) 2991
Mva1269I GAATGC 4 cut(s) 1257, 1552, 2223, 2356
MvaI CCWGG 5 cut(s) 273, 785, 1337, 1623, 2163
MvnI CGCG 1 cut(s) 1501
MwoI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2006, 2869, 2875, 2927
NcoI CCATGG 2 cut(s) 33, 1067
NdeI CATATG 1 cut(s) 514
NheI GCTAGC 1 cut(s) 1133
NlaIV GGNNCC 3 cut(s) 463, 520, 781
NmuCI GTSAC 4 cut(s) 643, 1678, 1808, 2285
NsiI ATGCAT 3 cut(s) 25, 886, 2358
NspI RCATGY 5 cut(s) 662, 1129, 1770, 2031, 2356
NspV TTCGAA 1 cut(s) 113
PaeI GCATGC 1 cut(s) 1770
PagI TCATGA 1 cut(s) 982
PciI ACATGT 2 cut(s) 1125, 2027
PciSI GCTCTTC 2 cut(s) 648, 1179
PctI GAATGC 4 cut(s) 1257, 1552, 2223, 2356
PdmI GAANNNNTTC 3 cut(s) 207, 246, 1669
PfeI GAWTC 8 cut(s) 52, 545, 862, 1249, 1665, 1708, 2081, 2613
Pfl23II CGTACG 1 cut(s) 768
PflMI CCANNNNNTGG 2 cut(s) 1184, 1622
PkrI GCNGC 7 cut(s) 412, 825, 1701, 1831, 2194, 2871, 3002
PleI GAGTC 5 cut(s) 772, 2045, 2064, 2200, 2596
PpsI GAGTC 5 cut(s) 772, 2045, 2064, 2200, 2596
Ppu21I YACGTR 2 cut(s) 1721, 2285
PpuMI RGGWCCY 1 cut(s) 779
PscI ACATGT 2 cut(s) 1125, 2027
PshBI ATTAAT 2 cut(s) 2100, 2895
Psp1406I AACGTT 2 cut(s) 1196, 2341
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 5 cut(s) 271, 783, 1335, 1621, 2161
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 5 cut(s) 271, 783, 1335, 1621, 2161
PspLI CGTACG 1 cut(s) 768
PspN4I GGNNCC 3 cut(s) 463, 520, 781
PspPI GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
PspPPI RGGWCCY 1 cut(s) 779
PsrI GAACNNNNNNTAC 2 cut(s) 2331, 2363
PstI CTGCAG 1 cut(s) 2539
PstNI CAGNNNCTG 1 cut(s) 1622
PsuI RGATCY 4 cut(s) 348, 2430, 2519, 2970
PvuII CAGCTG 3 cut(s) 2195, 2621, 2656
RsaI GTAC 9 cut(s) 99, 405, 770, 1408, 1614, 1627, 2597, 2652, 2813
RsaNI GTAC 9 cut(s) 98, 404, 769, 1407, 1613, 1626, 2596, 2651, 2812
SalI GTCGAC 1 cut(s) 1849
SapI GCTCTTC 2 cut(s) 648, 1179
SatI GCNGC 7 cut(s) 411, 824, 1700, 1830, 2193, 2870, 3001
Sau96I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
ScaI AGTACT 1 cut(s) 405
SchI GAGTC 5 cut(s) 773, 2045, 2064, 2200, 2597
ScrFI CCNGG 5 cut(s) 273, 785, 1337, 1623, 2163
SduI GDGCHC 3 cut(s) 496, 523, 1234
SexAI ACCWGGT 1 cut(s) 2161
SfcI CTRYAG 4 cut(s) 843, 1161, 1353, 2535
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
SmlI CTYRAG 5 cut(s) 1118, 1461, 2211, 2415, 2762
SmoI CTYRAG 5 cut(s) 1118, 1461, 2211, 2415, 2762
SphI GCATGC 1 cut(s) 1770
SsiI CCGC 2 cut(s) 2180, 3007
SspI AATATT 2 cut(s) 631, 1077
SspMI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2268, 2607
StyD4I CCNGG 5 cut(s) 271, 783, 1335, 1621, 2161
StyI CCWWGG 5 cut(s) 33, 1067, 1082, 1909, 2421
TaaI ACNGT 1 cut(s) 1855
TaiI ACGT 6 cut(s) 333, 1199, 1689, 1723, 2287, 2344
TaqI TCGA 6 cut(s) 113, 1821, 1850, 2724, 2747, 2948
TatI WGTACW 2 cut(s) 403, 2650
TfiI GAWTC 8 cut(s) 52, 545, 862, 1249, 1665, 1708, 2081, 2613
TscAI CASTG 6 cut(s) 82, 1860, 1950, 2508, 2544, 2686
TseFI GTSAC 4 cut(s) 643, 1678, 1808, 2285
TseI GCWGC 7 cut(s) 410, 823, 1699, 1829, 2192, 2869, 3000
Tsp45I GTSAC 4 cut(s) 643, 1678, 1808, 2285
TspGWI ACGGA 1 cut(s) 1112
TspRI CASTG 6 cut(s) 82, 1860, 1950, 2508, 2544, 2686
Van91I CCANNNNNTGG 2 cut(s) 1184, 1622
VneI GTGCAC 1 cut(s) 1230
VpaK11BI GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
VspI ATTAAT 2 cut(s) 2100, 2895
XapI RAATTY 7 cut(s) 115, 1024, 1106, 1181, 2169, 2311, 2688
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 5 cut(s) 662, 1129, 1770, 2031, 2356
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmiI GTMKAC 3 cut(s) 1850, 2676, 2737
XmnI GAANNNNTTC 3 cut(s) 207, 246, 1669
XspI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2268, 2607
ZrmI AGTACT 1 cut(s) 405
Zsp2I ATGCAT 3 cut(s) 25, 886, 2358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.