Rh5AG323200

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013644
Physical Location & Seq
Forward (+)
48284325 .. 48287818
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG323200.1

Sequence Viewer

Length: 3012 bp
ATGGCATCCTCTAGAGATGATGCATTGGCCACCATGGCTGTCAATAGAGTGAATCAACTTATGGAGGAAATAGGCACTGCAACCACCGATTTTCGGGTACTCATCCCTCATTCGAATTCTCAGCACTTTGTCTTAGGTCCAGCACTCAAATCTGACAATAGCTTCCCTGACATTTATAGTGATTTTCCCAAAACGAAAACTGAAGATTTTCTTATACATGATCAAAATGCCAAGTTCTATGAATGGTTTCATCAGAAGCATCGGTTTTGGCCTGGCAATGATCCTGATTGGGTCTCTTGGTTTAAAAGAGTTGAAGCTCAAAAGAGTTACGTTTGGCATCAAGCTGGGATCTATGACATTCTCCAGTTCTCTAAGTTTACCATTCCTCTTAATCGTTCTTTAGTACTAGCTGCACTCTGCTTTTGGTCAATTTCCACAAATTCTTTTCATTTTGGTCGTGGTCCCCTTACTATTACCCTCTTGGACCTTAGTGCTCTGGTAGGACTTAGACCATGTGGTGCCCAATTTGATGCTGTCGTTGCTGATTCCATCTCCATCCCAGAGGATAATTATGGCATTGAAGTTAATCAGTTCTCCAGTTTTCCTTCATTTATTAGGGAACATAAAAATATTGAAGGAGCTGTGACAGATGAAGAGCATGTAGCATTTCTTCACTATTGGCTTTGCCGTTACCTTCTTTGTTCTCCATCATTCAAGATCATCAAAACCAATTTGCCTTTGGCTGTGGCTTTGTCTAGGGGTGAGTCGTACGCCTTAGGACCCCTGGTTCTTGCCTACTTGTATAGAGGCATCAAGGACTTGCTGCACTCTCAGCTAAATACTACAGGTGGACCAATATGGATTCTTCAACTTTGGCTTCATGCATACTTTCCCAAAATGCGTCCAGATTGTTTTGCAATTCCCAACCTCTCCTGTCATGGACTAAAATATGTTTCTTTCAAACAGCCTAAACTTTTGTTTCATGATTGTTTATTGTATATGAGCTACTTAGACAGAGAAGAGAATTTCAACTTCAATCCATATTCAAAGTTAGATGCTTTACCTCCATGGTTAATATTTCCTTGGACTGAAATGACGGAAACTGAATTTTCTACACTCACGGACATGTGGGCTAGCTTTCTTGTTAGTCGTGATCTTCCTGTAGGTATAGCTCTTCCAAAATTTGGAAACAAAACGTTTGGTTTTGAAGCATATTGCCCAAATCTTTGTGCACGCCAGTTTGGGCTGATTCAAGGCATTCCTACTCCTCCTTTCTTCTCTCTTAATACCCATTTCCCAGAAAAGATAGTTGTTCGCAGTTTTGAAGAACAACTCCAGGTGCTTAAAGTTACTACAGCTCATTTGTTCACAAAACTTGAACTTTCTTCATATTCACCACTACCTAGTACCACCTCATCCTTCAAAGCTTGGTGGACCAAATACTATAAGCACACCTTTCCTCAAGATCTGAACTATCTTGAAATGCTTGATGACTTGACCCGAAACAGAACGCCTCGACAAGAGCCATCAGAGAAAGCAAGAAAGAATGCTTTGAGGAAGCGCAAAGGGGTTATCACCATTCAAGACCCCAGTTCTCACCAACAGCGAGTACCAGACCCTGGTACATCAAGAAAAAGGATTAGGTATGTAACTAAGCAAGGAATCTGTTCAAGTGTGACAGACGGAAAAGGAATGCTGCCTATGATTCAATGCCTACGTGGACAGGGCAGAGTTCATAGCAAGACAAGAATGACTATTAAAGCATGCCATATTTTGAGAAGTATAAGAAAGGGAAATTATTTATGTCACACCAAGTTCGAGGAAGCTGCTACTAAGACAAAGAGGTCGACAGTGTGTAAGAACAAACAAGCTAATTGCTCATCTAAAAATCAATTCCAGAGTGGCCAAGGGAAAAGTTCTGGCAACACACCGAAAGGAGCAGTGCATTGTTATTTGATAAATCTGACACATTCCCATTCTCAGTCTCTCTCTGAGGCCACCAAAGCCACCTTCCAAAAGAAAACATGTCCTCAGTCAAAGCATCAAGACTCTGATGCTGTATGTGGACTCGTTGTTGAATCAACTGAAGATAACATTAATTTTCAGGTTAATGATCCTCCACTAACAGAGACTATGGATGCTTCAAATGAGAAGCTACCTGGTCAAATTTCATCTCCACCAACAGAAGCAGCTGAGCCTCAGACTCCTCAAGAGCCAAGCATTCAATTCCCATTCCATGCACCTTCATCTCATCCCAACCCTTCTAGTTCTCATACCATACGTGACCTTGAGGTGTCAAATCCTACAAATTCTATACGAGCTTTGGAGTTTCTGGAACGTTATCTACACATGCATTCAGATATTGGTATCAAAACTGAAGAAACTCCCTTTCCTAAATCCGAAGAAATTTCTCAAGCCAAGGTCAAGATCCAAAGTATTTCAACACTTCCTCTGGATAAAGTCATGGAAACTGAATGTTTGCAAAGCTTCAAATCATCACTGGAAACTTTGCTCAGATCTAATGACCAAACTGCAGTGCAGTTGGCAAACATTGAAGATGTCCTGAGAAAGCTTGCTTTTCTGGAGTCCAAGTACCCCTCAGCTAGTGGAATCACAGCTGAAGTTAACAAAGCATTTGTTCAGAAAGTACAGCTGGAACAGGAAGTGGAAGTCTACACTGGCCAAATTTCTGAAGCTAAAAAAGAAGAGGCAAAGTTTTTCAAGTGTGTAAGTATACAGAAGTCGAGAGTTGAGGAACTTGAGACAAAATTGGCTGAAGAGAAAGCTACTTTAATTGCATTGGAGAAGTACCATGCCACCTACAAAAGCAAAATGGATGAATTGAATTACAAGTGTGAGCAAGTGCAGCAGATGCTGTGTCATCATAGTATTAATGAGAGAACTTTGGTGAACAAAGCTTTGCAAGCAAGAGGTGATTTTAAATCGATGGAAGCAACTTGGGTTCAGATCCGGCATCTTGTTTCCTCAATTGGTGGCAGCAAGCGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1003

Amino Acids

113.93

Weight (kDa)

8.54

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 116 - 478 1.9e-44 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g26742 FvH4_3g26742
malus_domestica MD11G1175200.v1.1
pyrus_communis pycom11g15030
rosa_chinensis RchiOBHm_Chr5g0049171
rosa_laevigata RLG00000034596
rosa_multiflora Rmu_sc0001677.1_g000029
rosa_roxburghii Rroxscaffold_1G00032560
rosa_rugosa Rorug05G0244500 Rorug05G0244600 Rorug05G0244700 Rorug05G0244800 Rorug05G0244900
rosa_samantha Rh5AG323200 Rh5BG333700 Rh5CG358800 Rh5DG345200
rosa_wichuraiana Rw5G030510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1843
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 2 cut(s) 1184, 1619
AccI GTMKAC 3 cut(s) 1847, 2673, 2734
AciI CCGC 1 cut(s) 3004
AclI AACGTT 2 cut(s) 1196, 2338
AclWI GGATC 5 cut(s) 275, 356, 2108, 2422, 2962
AcoI YGGCCR 3 cut(s) 27, 1903, 2680
AcsI RAATTY 9 cut(s) 115, 439, 1024, 1106, 1181, 2166, 2308, 2406, 2685
AcuI CTGAAG 6 cut(s) 222, 2106, 2397, 2640, 2712, 2796
AfaI GTAC 9 cut(s) 99, 405, 770, 1408, 1611, 1624, 2594, 2649, 2810
AfiI CCNNNNNNNGG 3 cut(s) 93, 1184, 1619
AflIII ACRYGT 2 cut(s) 1125, 2024
AjnI CCWGG 5 cut(s) 271, 783, 1335, 1618, 2158
AjuI GAANNNNNNNTTGG 2 cut(s) 1994, 2026
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
Alw21I GWGCWC 2 cut(s) 496, 1234
Alw26I GTCTC 4 cut(s) 298, 1989, 2123, 2756
Alw44I GTGCAC 1 cut(s) 1230
AlwI GGATC 5 cut(s) 275, 356, 2108, 2422, 2962
AlwNI CAGNNNCTG 2 cut(s) 1619, 2875
AoxI GGCC 5 cut(s) 27, 269, 1903, 1995, 2680
ApaLI GTGCAC 1 cut(s) 1230
ApeKI GCWGC 7 cut(s) 410, 823, 1696, 1826, 2189, 2866, 2997
ApoI RAATTY 9 cut(s) 115, 439, 1024, 1106, 1181, 2166, 2308, 2406, 2685
AseI ATTAAT 2 cut(s) 2097, 2892
Asp700I GAANNNNTTC 3 cut(s) 207, 246, 1666
AspLEI GCGC 1 cut(s) 1563
AspS9I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
AsuHPI GGTGA 6 cut(s) 773, 1386, 1567, 1589, 2920, 2945
AsuII TTCGAA 1 cut(s) 113
AsuNHI GCTAGC 1 cut(s) 1133
AvaII GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
AxyI CCTNAGG 1 cut(s) 775
BaeGI GKGCMC 2 cut(s) 523, 1234
BalI TGGCCA 3 cut(s) 29, 1905, 2682
BanI GGYRCC 1 cut(s) 518
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
Bbv12I GWGCWC 2 cut(s) 496, 1234
BbvCI CCTCAGC 1 cut(s) 2599
BbvI GCAGC 6 cut(s) 397, 810, 1683, 1813, 2201, 2878
BccI CCATC 5 cut(s) 557, 563, 715, 1534, 2941
BceAI ACGGC 1 cut(s) 672
BcgI CGANNNNNNTGC 2 cut(s) 1808, 1842
BciT130I CCWGG 5 cut(s) 273, 785, 1337, 1620, 2160
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 4 cut(s) 298, 1989, 2123, 2756
BfaI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2265, 2604
BfmI CTRYAG 4 cut(s) 843, 1161, 1353, 2532
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 2 cut(s) 1465, 2516
BisI GCNGC 7 cut(s) 411, 824, 1697, 1827, 2190, 2867, 2998
BlpI GCTNAGC 1 cut(s) 2193
BlsI GCNGC 7 cut(s) 412, 825, 1698, 1828, 2191, 2868, 2999
BmcAI AGTACT 1 cut(s) 405
Bme1390I CCNGG 5 cut(s) 273, 785, 1337, 1620, 2160
Bme18I GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
BmgT120I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
BmiI GGNNCC 3 cut(s) 463, 520, 781
BmrFI CCNGG 5 cut(s) 273, 785, 1337, 1620, 2160
BmrI ACTGGG 1 cut(s) 1584
BmtI GCTAGC 1 cut(s) 1137
BmuI ACTGGG 1 cut(s) 1584
BpmI CTGGAG 4 cut(s) 347, 580, 1319, 2603
Bpu10I CCTNAGC 1 cut(s) 2599
Bpu1102I GCTNAGC 1 cut(s) 2193
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 5 cut(s) 1446, 2193, 2309, 2397, 2780
Bsa29I ATCGAT 1 cut(s) 2945
BsaAI YACGTR 2 cut(s) 1718, 2282
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 7 cut(s) 33, 783, 1067, 1082, 1618, 1906, 2418
BsaXI ACNNNNNCTCC 2 cut(s) 2576, 2606
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 1184, 1619
Bse1I ACTGG 6 cut(s) 364, 597, 1237, 1590, 2505, 2683
Bse21I CCTNAGG 1 cut(s) 775
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 5 cut(s) 273, 785, 1337, 1620, 2160
BseCI ATCGAT 1 cut(s) 2945
BseDI CCNNGG 7 cut(s) 33, 783, 1067, 1082, 1618, 1906, 2418
BseGI GGATG 7 cut(s) 5, 102, 555, 1415, 2143, 2251, 2842
BseLI CCNNNNNNNGG 3 cut(s) 93, 1184, 1619
BseMI GCAATG 1 cut(s) 283
BseNI ACTGG 6 cut(s) 364, 597, 1237, 1590, 2505, 2683
BseRI GAGGAG 2 cut(s) 1257, 2196
BseSI GKGCMC 2 cut(s) 523, 1234
BseXI GCAGC 6 cut(s) 397, 810, 1683, 1813, 2201, 2878
BseYI CCCAGC 1 cut(s) 344
BsgI GTGCAG 4 cut(s) 396, 809, 2558, 2885
BshFI GGCC 5 cut(s) 29, 271, 1905, 1997, 2682
BshNI GGYRCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 2945
BsiHKAI GWGCWC 2 cut(s) 496, 1234
BsiSI CCGG 1 cut(s) 2971
BsiWI CGTACG 1 cut(s) 768
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 3 cut(s) 93, 1184, 1619
BsmAI GTCTC 4 cut(s) 298, 1989, 2123, 2756
BsmFI GGGAC 1 cut(s) 447
BsmI GAATGC 5 cut(s) 1257, 1552, 1698, 2220, 2353
BsnI GGCC 5 cut(s) 29, 271, 1905, 1997, 2682
Bso31I GGTCTC 1 cut(s) 298
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 496, 523, 1234
Bsp1720I GCTNAGC 1 cut(s) 2193
Bsp19I CCATGG 2 cut(s) 33, 1067
BspACI CCGC 1 cut(s) 3004
BspANI GGCC 5 cut(s) 29, 271, 1905, 1997, 2682
BspDI ATCGAT 1 cut(s) 2945
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 3 cut(s) 463, 520, 781
BspMAI CTGCAG 1 cut(s) 2536
BspOI GCTAGC 1 cut(s) 1137
BspPI GGATC 5 cut(s) 275, 356, 2108, 2422, 2962
BspQI GCTCTTC 2 cut(s) 648, 1179
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 6 cut(s) 364, 597, 1237, 1590, 2505, 2683
BssECI CCNNGG 7 cut(s) 33, 783, 1067, 1082, 1618, 1906, 2418
BssNAI GTATAC 1 cut(s) 2735
BssT1I CCWWGG 5 cut(s) 33, 1067, 1082, 1906, 2418
Bst1107I GTATAC 1 cut(s) 2735
Bst2UI CCWGG 5 cut(s) 273, 785, 1337, 1620, 2160
Bst4CI ACNGT 1 cut(s) 1852
Bst6I CTCTTC 5 cut(s) 648, 1014, 1179, 2700, 2772
BstAPI GCANNNNNTGC 1 cut(s) 2872
BstBAI YACGTR 2 cut(s) 1718, 2282
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 6 cut(s) 1135, 1234, 1765, 2574, 2925, 3002
BstDSI CCRYGG 2 cut(s) 33, 1067
BstF5I GGATG 7 cut(s) 5, 102, 555, 1415, 2143, 2251, 2842
BstHHI GCGC 1 cut(s) 1563
BstMAI GTCTC 4 cut(s) 298, 1989, 2123, 2756
BstMWI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2003, 2866, 2872, 2924
BstNI CCWGG 5 cut(s) 273, 785, 1337, 1620, 2160
BstNSI RCATGY 5 cut(s) 662, 1129, 1767, 2028, 2353
BstSCI CCNGG 5 cut(s) 271, 783, 1335, 1618, 2158
BstSFI CTRYAG 4 cut(s) 843, 1161, 1353, 2532
BstSLI GKGCMC 2 cut(s) 523, 1234
BstV1I GCAGC 6 cut(s) 397, 810, 1683, 1813, 2201, 2878
BstX2I RGATCY 5 cut(s) 348, 1465, 2427, 2516, 2967
BstYI RGATCY 5 cut(s) 348, 1465, 2427, 2516, 2967
BstZ17I GTATAC 1 cut(s) 2735
Bsu15I ATCGAT 1 cut(s) 2945
Bsu36I CCTNAGG 1 cut(s) 775
BsuRI GGCC 5 cut(s) 29, 271, 1905, 1997, 2682
BsuTUI ATCGAT 1 cut(s) 2945
BtgI CCRYGG 2 cut(s) 33, 1067
BtsCI GGATG 7 cut(s) 5, 102, 555, 1415, 2143, 2251, 2842
BtsI GCAGTG 3 cut(s) 75, 1947, 2541
BtsIMutI CAGTG 6 cut(s) 75, 1857, 1947, 2498, 2541, 2676
Cac8I GCNNGC 6 cut(s) 1135, 1234, 1765, 2574, 2925, 3002
CaiI CAGNNNCTG 2 cut(s) 1619, 2875
CciI TCATGA 1 cut(s) 982
CfoI GCGC 1 cut(s) 1563
Cfr13I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
ClaI ATCGAT 1 cut(s) 2945
CseI GACGC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 2158
Csp6I GTAC 9 cut(s) 98, 404, 769, 1407, 1610, 1623, 2593, 2648, 2809
CviQI GTAC 9 cut(s) 98, 404, 769, 1407, 1610, 1623, 2593, 2648, 2809
DraI TTTAAA 2 cut(s) 304, 2941
DrdI GACNNNNNNGTC 1 cut(s) 1843
DseDI GACNNNNNNGTC 1 cut(s) 1843
EaeI YGGCCR 3 cut(s) 27, 1903, 2680
Eam1104I CTCTTC 5 cut(s) 648, 1014, 1179, 2700, 2772
EarI CTCTTC 5 cut(s) 648, 1014, 1179, 2700, 2772
Eco130I CCWWGG 5 cut(s) 33, 1067, 1082, 1906, 2418
Eco31I GGTCTC 1 cut(s) 298
Eco47I GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
Eco57I CTGAAG 6 cut(s) 222, 2106, 2397, 2640, 2712, 2796
Eco81I CCTNAGG 1 cut(s) 775
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 5 cut(s) 271, 783, 1335, 1618, 2158
EcoT14I CCWWGG 5 cut(s) 33, 1067, 1082, 1906, 2418
EcoT22I ATGCAT 3 cut(s) 25, 886, 2355
ErhI CCWWGG 5 cut(s) 33, 1067, 1082, 1906, 2418
FalI AAGNNNNNCTT 4 cut(s) 195, 227, 1439, 1471
FaqI GGGAC 1 cut(s) 447
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 3 cut(s) 1847, 2673, 2734
Fnu4HI GCNGC 7 cut(s) 411, 824, 1697, 1827, 2190, 2867, 2998
FokI GGATG 6 cut(s) 89, 542, 1402, 2150, 2238, 2849
Fsp4HI GCNGC 7 cut(s) 411, 824, 1697, 1827, 2190, 2867, 2998
FspBI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2265, 2604
GlaI GCGC 1 cut(s) 1562
GluI GCNGC 7 cut(s) 411, 824, 1697, 1827, 2190, 2867, 2998
GsaI CCCAGC 1 cut(s) 348
GsuI CTGGAG 4 cut(s) 347, 580, 1319, 2603
HaeIII GGCC 5 cut(s) 29, 271, 1905, 1997, 2682
HapII CCGG 1 cut(s) 2971
HgaI GACGC 1 cut(s) 890
HhaI GCGC 1 cut(s) 1563
Hin6I GCGC 1 cut(s) 1561
HinP1I GCGC 1 cut(s) 1561
HincII GTYRAC 2 cut(s) 1848, 2626
HindII GTYRAC 2 cut(s) 1848, 2626
HindIII AAGCTT 4 cut(s) 1425, 2485, 2570, 2916
HpaI GTTAAC 1 cut(s) 2626
HpaII CCGG 1 cut(s) 2971
HphI GGTGA 6 cut(s) 773, 1386, 1567, 1589, 2920, 2945
HpyAV CCTTC 7 cut(s) 615, 629, 704, 1429, 2020, 2253, 2271
HpyCH4III ACNGT 1 cut(s) 1852
HpyCH4IV ACGT 5 cut(s) 330, 1196, 1717, 2281, 2338
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2003, 2866, 2872, 2924
HpySE526I ACGT 5 cut(s) 330, 1196, 1717, 2281, 2338
HspAI GCGC 1 cut(s) 1561
Ksp22I TGATCA 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 2626
LguI GCTCTTC 2 cut(s) 648, 1179
LmnI GCTCC 2 cut(s) 638, 1937
Lsp1109I GCAGC 6 cut(s) 397, 810, 1683, 1813, 2201, 2878
MabI ACCWGGT 1 cut(s) 2158
MaeI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2265, 2604
MaeII ACGT 5 cut(s) 330, 1196, 1717, 2281, 2338
MaeIII GTNAC 8 cut(s) 326, 643, 689, 1348, 1648, 1675, 1805, 2282
MfeI CAATTG 1 cut(s) 2988
MflI RGATCY 5 cut(s) 348, 1465, 2427, 2516, 2967
MhlI GDGCHC 3 cut(s) 496, 523, 1234
MlsI TGGCCA 3 cut(s) 29, 1905, 2682
MluNI TGGCCA 3 cut(s) 29, 1905, 2682
MlyI GAGTC 5 cut(s) 773, 2042, 2061, 2197, 2594
Mox20I TGGCCA 3 cut(s) 29, 1905, 2682
Mph1103I ATGCAT 3 cut(s) 25, 886, 2355
MroXI GAANNNNTTC 3 cut(s) 207, 246, 1666
MscI TGGCCA 3 cut(s) 29, 1905, 2682
MslI CAYNNNNRTG 1 cut(s) 1124
Msp20I TGGCCA 3 cut(s) 29, 1905, 2682
MspA1I CMGCKG 3 cut(s) 2192, 2618, 2653
MspI CCGG 1 cut(s) 2971
MspR9I CCNGG 5 cut(s) 273, 785, 1337, 1620, 2160
MunI CAATTG 1 cut(s) 2988
Mva1269I GAATGC 5 cut(s) 1257, 1552, 1698, 2220, 2353
MvaI CCWGG 5 cut(s) 273, 785, 1337, 1620, 2160
MwoI GCNNNNNNNGC 7 cut(s) 35, 539, 832, 2003, 2866, 2872, 2924
NcoI CCATGG 2 cut(s) 33, 1067
NheI GCTAGC 1 cut(s) 1133
NlaIV GGNNCC 3 cut(s) 463, 520, 781
NmuCI GTSAC 4 cut(s) 643, 1675, 1805, 2282
NsiI ATGCAT 3 cut(s) 25, 886, 2355
NspI RCATGY 5 cut(s) 662, 1129, 1767, 2028, 2353
NspV TTCGAA 1 cut(s) 113
PaeI GCATGC 1 cut(s) 1767
PagI TCATGA 1 cut(s) 982
PciI ACATGT 2 cut(s) 1125, 2024
PciSI GCTCTTC 2 cut(s) 648, 1179
PctI GAATGC 5 cut(s) 1257, 1552, 1698, 2220, 2353
PdmI GAANNNNTTC 3 cut(s) 207, 246, 1666
PfeI GAWTC 8 cut(s) 52, 545, 862, 1249, 1662, 1705, 2078, 2610
Pfl23II CGTACG 1 cut(s) 768
PflMI CCANNNNNTGG 2 cut(s) 1184, 1619
PkrI GCNGC 7 cut(s) 412, 825, 1698, 1828, 2191, 2868, 2999
PleI GAGTC 5 cut(s) 772, 2042, 2061, 2197, 2593
PpsI GAGTC 5 cut(s) 772, 2042, 2061, 2197, 2593
Ppu21I YACGTR 2 cut(s) 1718, 2282
PpuMI RGGWCCY 1 cut(s) 779
PscI ACATGT 2 cut(s) 1125, 2024
PshBI ATTAAT 2 cut(s) 2097, 2892
Psp1406I AACGTT 2 cut(s) 1196, 2338
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 5 cut(s) 271, 783, 1335, 1618, 2158
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 5 cut(s) 271, 783, 1335, 1618, 2158
PspLI CGTACG 1 cut(s) 768
PspN4I GGNNCC 3 cut(s) 463, 520, 781
PspPI GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
PspPPI RGGWCCY 1 cut(s) 779
PsrI GAACNNNNNNTAC 2 cut(s) 2328, 2360
PstI CTGCAG 1 cut(s) 2536
PstNI CAGNNNCTG 2 cut(s) 1619, 2875
PsuI RGATCY 5 cut(s) 348, 1465, 2427, 2516, 2967
PvuII CAGCTG 3 cut(s) 2192, 2618, 2653
RsaI GTAC 9 cut(s) 99, 405, 770, 1408, 1611, 1624, 2594, 2649, 2810
RsaNI GTAC 9 cut(s) 98, 404, 769, 1407, 1610, 1623, 2593, 2648, 2809
RseI CAYNNNNRTG 1 cut(s) 1124
SalI GTCGAC 1 cut(s) 1846
SapI GCTCTTC 2 cut(s) 648, 1179
SatI GCNGC 7 cut(s) 411, 824, 1697, 1827, 2190, 2867, 2998
Sau96I GGNCC 6 cut(s) 137, 461, 484, 779, 851, 1434
ScaI AGTACT 1 cut(s) 405
SchI GAGTC 5 cut(s) 773, 2042, 2061, 2197, 2594
ScrFI CCNGG 5 cut(s) 273, 785, 1337, 1620, 2160
SduI GDGCHC 3 cut(s) 496, 523, 1234
SexAI ACCWGGT 1 cut(s) 2158
SfcI CTRYAG 4 cut(s) 843, 1161, 1353, 2532
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
SmiMI CAYNNNNRTG 1 cut(s) 1124
SmlI CTYRAG 5 cut(s) 1461, 2208, 2288, 2412, 2759
SmoI CTYRAG 5 cut(s) 1461, 2208, 2288, 2412, 2759
SphI GCATGC 1 cut(s) 1767
SsiI CCGC 1 cut(s) 3004
SspI AATATT 2 cut(s) 631, 1077
SspMI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2265, 2604
StyD4I CCNGG 5 cut(s) 271, 783, 1335, 1618, 2158
StyI CCWWGG 5 cut(s) 33, 1067, 1082, 1906, 2418
TaaI ACNGT 1 cut(s) 1852
TaiI ACGT 5 cut(s) 333, 1199, 1720, 2284, 2341
TaqI TCGA 6 cut(s) 113, 1516, 1818, 1847, 2744, 2945
TatI WGTACW 2 cut(s) 403, 2647
TfiI GAWTC 8 cut(s) 52, 545, 862, 1249, 1662, 1705, 2078, 2610
TscAI CASTG 6 cut(s) 82, 1857, 1947, 2505, 2541, 2683
TseFI GTSAC 4 cut(s) 643, 1675, 1805, 2282
TseI GCWGC 7 cut(s) 410, 823, 1696, 1826, 2189, 2866, 2997
Tsp45I GTSAC 4 cut(s) 643, 1675, 1805, 2282
TspGWI ACGGA 3 cut(s) 1112, 1136, 1698
TspRI CASTG 6 cut(s) 82, 1857, 1947, 2505, 2541, 2683
Van91I CCANNNNNTGG 2 cut(s) 1184, 1619
VneI GTGCAC 1 cut(s) 1230
VpaK11BI GGWCC 6 cut(s) 137, 461, 484, 779, 851, 1434
VspI ATTAAT 2 cut(s) 2097, 2892
XapI RAATTY 9 cut(s) 115, 439, 1024, 1106, 1181, 2166, 2308, 2406, 2685
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 5 cut(s) 662, 1129, 1767, 2028, 2353
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmiI GTMKAC 3 cut(s) 1847, 2673, 2734
XmnI GAANNNNTTC 3 cut(s) 207, 246, 1666
XspI CTAG 7 cut(s) 12, 407, 756, 1134, 1404, 2265, 2604
ZrmI AGTACT 1 cut(s) 405
Zsp2I ATGCAT 3 cut(s) 25, 886, 2355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.