RchiOBHm_Chr6g0282821

chloroplast organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012361
Physical Location & Seq
Reverse (-)
46067533 .. 46071311
3779 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25358

Sequence Viewer

Length: 1206 bp
ATGTTGCGGAGCACCACCATATTCTTCCCCACCTGTTCATATTCACCATCACAATCTCTCCGCCCAACCACTATCTCTCCCTGTCTCCTCTCTCTCCCATCATTTTCCTCTCACCACACACAATCCCAGTGGCATCCAAATCAACCAGCCCACAAGAAGAGCCAATTTCAGCGACTCTGTGGCACTTATGAGGTCGGTGGAGGCTTCCCAGACGACGGATTGGGCGTCCAAGACAGGAACAGAACTACCCAAGAGAAATTGGACCCGGCCCAGATTGAAGCCCTACTCAAAGGTGGAGAGCAAGTCACTTCTGTCCTCGAAGAAATGATCACCCTCTTGGAGGATATGAAGATGGATGAGGCGTCTGAAGAGGTGGCAGTGGAAATAGCTGCACAAGGGGTCCTGGGGAAGAGGGTTGATGAGATGGAGGCCGGTTTCATGATGGCGCTGGACTACATGATCCAACTTGCTGACACTGACCGAGACGAGAAGCGCAAGTCTCTATTGGAGGTGATTAAGGACACAGTCTTATCCTACCTTACTAAAAAATGCCCCCCGCATGTTCAAGTGATTGGGCTACTCTGTAGAACTCCTCTGAAAGAAAGTAGACATGAATTACTACGCAGAGTGGCTGCTGGTGGTGGTGTATTTAAAAGTAAAAATGACGTCAAAGTTCATGTTCCAGCTGCAAATCTAAATGAAATTGCTAACCAAGCTGATGATTTACTGGAGACAATGGAAACTCGGCCTATAGTTCCGGATCGAAAGCTACTTGCAAGACTTGTTTTAATCAGAGAGGAAGCTCGGAATATGATGGGAGGTGGAATACTAGATGAGAGAAATGATCGTGGGTTTAAGACTCTTCCTGAATCAGAGGTGAACTTCTTAACCAAACTGGTAGCTTTGAAACCGGGGAAGACTGTGGAGGAGATGATAAAAAGTGTAATGGAAGGAAAAGACGAAGGTGCAGAAGACTCTGGCAGTGATATGGAAGATACCACCAGTAGAAAGGGTTCGAGTGGAATTGCAGGAAGGGAAAGTGTTACAGGACGAAAAGCGCTTCCTGTGCGCCCTGGCATGTTTCTTGAGACTGTATCCAAGGTCTTAGGTGGTATATATGCTGGAAATGTCTCTGGCATCACAGCACAACATCTAGAGTGGGTTCATCAAAAGACACTTGAAGTTCTTCAGGAAATAGCATTTTAA

Protein Analysis

401

Amino Acids

44.25

Weight (kDa)

5.63

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48470
fragaria_vesca FvH4_2g17800 FvH4_2g17800 FvH4_2g17800
malus_domestica MD05G1159400.v1.1 MD10G1151900.v1.1
prunus_persica Prupe.8G190300_v2.0.a1 Prupe.8G190300_v2.0.a1
pyrus_communis pycom10g13090
rosa_chinensis RchiOBHm_Chr6g0282821
rosa_laevigata RLG00000012865
rosa_multiflora Rmu_sc0006070.1_g000012
rosa_roxburghii Rroxscaffold_7G00185970
rosa_rugosa Rorug06G0149800
rosa_samantha Rh6AG263100 Rh6BG266000 Rh6CG265200 Rh6DG257800
rosa_wichuraiana Rw6G022710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 669
AccI GTMKAC 1 cut(s) 607
AccIII TCCGGA 1 cut(s) 757
AciI CCGC 3 cut(s) 7, 61, 557
AclWI GGATC 2 cut(s) 454, 768
AcuI CTGAAG 2 cut(s) 387, 1172
AcyI GRCGYC 3 cut(s) 225, 362, 666
AfeI AGCGCT 1 cut(s) 1059
AfiI CCNNNNNNNGG 2 cut(s) 215, 340
AgsI TTSAA 4 cut(s) 278, 566, 907, 1181
AjnI CCWGG 2 cut(s) 402, 1072
AluBI AGCT 6 cut(s) 389, 686, 716, 769, 803, 902
AluI AGCT 6 cut(s) 389, 686, 716, 769, 803, 902
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 6 cut(s) 89, 477, 504, 725, 1082, 1135
AlwI GGATC 2 cut(s) 454, 768
Aor13HI TCCGGA 1 cut(s) 757
Aor51HI AGCGCT 1 cut(s) 1059
AoxI GGCC 3 cut(s) 267, 429, 746
ApeKI GCWGC 3 cut(s) 389, 632, 686
Asp700I GAANNNNTTC 2 cut(s) 1012, 1185
AspLEI GCGC 4 cut(s) 448, 495, 1060, 1071
AspS9I GGNCC 3 cut(s) 262, 268, 400
AsuC2I CCSGG 2 cut(s) 266, 912
AsuHPI GGTGA 5 cut(s) 36, 104, 322, 523, 889
AvaII GGWCC 2 cut(s) 262, 400
BbsI GAAGAC 2 cut(s) 923, 978
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 3 cut(s) 376, 619, 673
BccI CCATC 6 cut(s) 55, 106, 346, 418, 436, 808
BciT130I CCWGG 2 cut(s) 404, 1074
BciVI GTATCC 1 cut(s) 1105
BclI TGATCA 1 cut(s) 327
BcnI CCSGG 2 cut(s) 266, 912
BcoDI GTCTC 6 cut(s) 89, 477, 504, 725, 1082, 1135
BfaI CTAG 2 cut(s) 830, 1154
BfmI CTRYAG 2 cut(s) 583, 750
BfoI RGCGCY 2 cut(s) 449, 1061
BfuI GTATCC 1 cut(s) 1105
BisI GCNGC 3 cut(s) 390, 633, 687
BlsI GCNGC 3 cut(s) 391, 634, 688
Bme1390I CCNGG 4 cut(s) 266, 404, 912, 1074
Bme18I GGWCC 2 cut(s) 262, 400
BmgT120I GGNCC 3 cut(s) 262, 268, 400
BmiI GGNNCC 2 cut(s) 264, 401
BmrFI CCNGG 4 cut(s) 266, 404, 912, 1074
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 2 cut(s) 142, 1146
BmuI ACTGGG 1 cut(s) 121
BpiI GAAGAC 2 cut(s) 923, 978
BpmI CTGGAG 1 cut(s) 749
BpuEI CTTGAG 1 cut(s) 1106
BpuMI CCSGG 2 cut(s) 266, 912
BsaHI GRCGYC 3 cut(s) 225, 362, 666
BsaJI CCNNGG 4 cut(s) 403, 911, 1072, 1098
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 6 cut(s) 42, 61, 72, 91, 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 340
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 4 cut(s) 127, 732, 900, 1002
BseAI TCCGGA 1 cut(s) 757
BseBI CCWGG 2 cut(s) 404, 1074
BseDI CCNNGG 4 cut(s) 403, 911, 1072, 1098
BseGI GGATG 2 cut(s) 133, 361
BseLI CCNNNNNNNGG 2 cut(s) 215, 340
BseNI ACTGG 4 cut(s) 127, 732, 900, 1002
BseRI GAGGAG 3 cut(s) 77, 582, 941
BseXI GCAGC 3 cut(s) 376, 619, 673
BsgI GTGCAG 2 cut(s) 375, 987
BshFI GGCC 3 cut(s) 269, 431, 748
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 4 cut(s) 266, 432, 758, 911
BslI CCNNNNNNNGG 2 cut(s) 215, 340
BsmAI GTCTC 6 cut(s) 89, 477, 504, 725, 1082, 1135
BsmBI CGTCTC 1 cut(s) 477
BsnI GGCC 3 cut(s) 269, 431, 748
Bsp1286I GDGCHC 1 cut(s) 14
Bsp13I TCCGGA 1 cut(s) 757
Bsp143I GATC 4 cut(s) 327, 459, 760, 844
BspACI CCGC 3 cut(s) 7, 61, 557
BspANI GGCC 3 cut(s) 269, 431, 748
BspEI TCCGGA 1 cut(s) 757
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 264, 401
BspPI GGATC 2 cut(s) 454, 768
BspQI GCTCTTC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 4 cut(s) 127, 732, 900, 1002
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 4 cut(s) 403, 911, 1072, 1098
BssMI GATC 4 cut(s) 327, 459, 760, 844
BssNI GRCGYC 3 cut(s) 225, 362, 666
BssT1I CCWWGG 1 cut(s) 1098
Bst2UI CCWGG 2 cut(s) 404, 1074
Bst4CI ACNGT 3 cut(s) 526, 922, 1093
Bst6I CTCTTC 4 cut(s) 152, 363, 404, 867
BstACI GRCGYC 3 cut(s) 225, 362, 666
BstDEI CTNAG 1 cut(s) 1105
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 2 cut(s) 133, 361
BstH2I RGCGCY 2 cut(s) 449, 1061
BstHHI GCGC 4 cut(s) 448, 495, 1060, 1071
BstKTI GATC 4 cut(s) 330, 462, 763, 847
BstMAI GTCTC 6 cut(s) 89, 477, 504, 725, 1082, 1135
BstMBI GATC 4 cut(s) 327, 459, 760, 844
BstMWI GCNNNNNNNGC 2 cut(s) 713, 1066
BstNI CCWGG 2 cut(s) 404, 1074
BstNSI RCATGY 2 cut(s) 563, 1081
BstSCI CCNGG 4 cut(s) 264, 402, 910, 1072
BstSFI CTRYAG 2 cut(s) 583, 750
BstV1I GCAGC 3 cut(s) 376, 619, 673
BstV2I GAAGAC 2 cut(s) 923, 978
BsuI GTATCC 1 cut(s) 1105
BsuRI GGCC 3 cut(s) 269, 431, 748
BtsCI GGATG 2 cut(s) 133, 361
BtsI GCAGTG 2 cut(s) 384, 988
BtsIMutI CAGTG 4 cut(s) 134, 384, 474, 988
CciI TCATGA 1 cut(s) 438
CfoI GCGC 4 cut(s) 448, 495, 1060, 1071
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 3 cut(s) 262, 268, 400
CseI GACGC 2 cut(s) 214, 351
CspCI CAANNNNNGTGG 2 cut(s) 110, 145
CviAII CATG 6 cut(s) 439, 457, 560, 611, 677, 1078
DdeI CTNAG 1 cut(s) 1105
DpnI GATC 4 cut(s) 329, 461, 762, 846
DpnII GATC 4 cut(s) 327, 459, 760, 844
DraI TTTAAA 1 cut(s) 652
Eam1104I CTCTTC 4 cut(s) 152, 363, 404, 867
EarI CTCTTC 4 cut(s) 152, 363, 404, 867
EciI GGCGGA 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 1098
Eco47I GGWCC 2 cut(s) 262, 400
Eco47III AGCGCT 1 cut(s) 1059
Eco57I CTGAAG 2 cut(s) 387, 1172
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 400
EcoRII CCWGG 2 cut(s) 402, 1072
EcoT14I CCWWGG 1 cut(s) 1098
ErhI CCWWGG 1 cut(s) 1098
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 6 cut(s) 442, 460, 563, 614, 680, 1081
FatI CATG 6 cut(s) 438, 456, 559, 610, 676, 1077
FauI CCCGC 1 cut(s) 564
FbaI TGATCA 1 cut(s) 327
FblI GTMKAC 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 390, 633, 687
FokI GGATG 2 cut(s) 120, 368
Fsp4HI GCNGC 3 cut(s) 390, 633, 687
FspBI CTAG 2 cut(s) 830, 1154
GlaI GCGC 4 cut(s) 447, 494, 1059, 1070
GluI GCNGC 3 cut(s) 390, 633, 687
GsuI CTGGAG 1 cut(s) 749
HaeII RGCGCY 2 cut(s) 449, 1061
HaeIII GGCC 3 cut(s) 269, 431, 748
HapII CCGG 4 cut(s) 266, 432, 758, 911
HgaI GACGC 2 cut(s) 214, 351
HhaI GCGC 4 cut(s) 448, 495, 1060, 1071
Hin1I GRCGYC 3 cut(s) 225, 362, 666
Hin1II CATG 6 cut(s) 442, 460, 563, 614, 680, 1081
Hin6I GCGC 4 cut(s) 446, 493, 1058, 1069
HinP1I GCGC 4 cut(s) 446, 493, 1058, 1069
HinfI GANTC 4 cut(s) 174, 859, 869, 974
HpaII CCGG 4 cut(s) 266, 432, 758, 911
HphI GGTGA 5 cut(s) 36, 104, 322, 523, 889
Hpy166II GTNNAC 2 cut(s) 608, 880
Hpy188I TCNGA 5 cut(s) 367, 597, 794, 807, 874
Hpy188III TCNNGA 6 cut(s) 439, 758, 866, 1085, 1154, 1190
Hpy8I GTNNAC 2 cut(s) 608, 880
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 944, 956, 1026
HpyCH4III ACNGT 3 cut(s) 526, 922, 1093
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 5 cut(s) 392, 689, 776, 968, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 713, 1066
HpyF3I CTNAG 1 cut(s) 1105
HpySE526I ACGT 1 cut(s) 666
Hsp92I GRCGYC 3 cut(s) 225, 362, 666
Hsp92II CATG 6 cut(s) 442, 460, 563, 614, 680, 1081
HspAI GCGC 4 cut(s) 446, 493, 1058, 1069
Kpn2I TCCGGA 1 cut(s) 757
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 4 cut(s) 327, 459, 760, 844
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 1 cut(s) 9
Lsp1109I GCAGC 3 cut(s) 376, 619, 673
LweI GCATC 2 cut(s) 142, 1146
MaeI CTAG 2 cut(s) 830, 1154
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 2 cut(s) 304, 1042
MalI GATC 4 cut(s) 329, 461, 762, 846
MboI GATC 4 cut(s) 327, 459, 760, 844
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 5 cut(s) 164, 257, 614, 702, 1023
MlyI GAGTC 3 cut(s) 168, 853, 968
MmeI TCCRAC 1 cut(s) 487
MroI TCCGGA 1 cut(s) 757
MroXI GAANNNNTTC 2 cut(s) 1012, 1185
MseI TTAA 6 cut(s) 516, 651, 788, 855, 887, 1204
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 4 cut(s) 266, 432, 758, 911
MspR9I CCNGG 4 cut(s) 266, 404, 912, 1074
MvaI CCWGG 2 cut(s) 404, 1074
MwoI GCNNNNNNNGC 2 cut(s) 713, 1066
NciI CCSGG 2 cut(s) 266, 912
NdeII GATC 4 cut(s) 327, 459, 760, 844
NlaIII CATG 6 cut(s) 442, 460, 563, 614, 680, 1081
NlaIV GGNNCC 2 cut(s) 264, 401
NmeAIII GCCGAG 1 cut(s) 724
NmuCI GTSAC 1 cut(s) 304
NspI RCATGY 2 cut(s) 563, 1081
PagI TCATGA 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 2 cut(s) 1012, 1185
PfeI GAWTC 1 cut(s) 869
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 3 cut(s) 391, 634, 688
PleI GAGTC 3 cut(s) 168, 853, 968
PpsI GAGTC 3 cut(s) 168, 853, 968
PpuMI RGGWCCY 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 2 cut(s) 402, 1072
PspGI CCWGG 2 cut(s) 402, 1072
PspN4I GGNNCC 2 cut(s) 264, 401
PspPI GGNCC 3 cut(s) 262, 268, 400
PspPPI RGGWCCY 1 cut(s) 400
PsrI GAACNNNNNNTAC 2 cut(s) 997, 1029
PsyI GACNNNGTC 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 686
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 6 cut(s) 516, 651, 788, 855, 887, 1204
SatI GCNGC 3 cut(s) 390, 633, 687
Sau3AI GATC 4 cut(s) 327, 459, 760, 844
Sau96I GGNCC 3 cut(s) 262, 268, 400
SchI GAGTC 3 cut(s) 168, 853, 968
ScrFI CCNGG 4 cut(s) 266, 404, 912, 1074
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 2 cut(s) 142, 1146
SfcI CTRYAG 2 cut(s) 583, 750
SinI GGWCC 2 cut(s) 262, 400
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
Sse9I AATT 5 cut(s) 164, 257, 614, 702, 1023
SsiI CCGC 3 cut(s) 7, 61, 557
SspMI CTAG 2 cut(s) 830, 1154
StyD4I CCNGG 4 cut(s) 264, 402, 910, 1072
StyI CCWWGG 1 cut(s) 1098
TaaI ACNGT 3 cut(s) 526, 922, 1093
TaiI ACGT 1 cut(s) 669
TaqI TCGA 3 cut(s) 318, 763, 1016
TaqII GACCGA 1 cut(s) 495
TasI AATT 5 cut(s) 164, 257, 614, 702, 1023
TfiI GAWTC 1 cut(s) 869
Tru1I TTAA 6 cut(s) 516, 651, 788, 855, 887, 1204
Tru9I TTAA 6 cut(s) 516, 651, 788, 855, 887, 1204
TscAI CASTG 4 cut(s) 134, 384, 481, 988
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 389, 632, 686
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 7 cut(s) 27, 362, 427, 627, 665, 714, 1154
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 4 cut(s) 134, 384, 481, 988
Tth111I GACNNNGTC 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 262, 400
XagI CCTNNNNNAGG 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 1153
XceI RCATGY 2 cut(s) 563, 1081
XmiI GTMKAC 1 cut(s) 607
XmnI GAANNNNTTC 2 cut(s) 1012, 1185
XspI CTAG 2 cut(s) 830, 1154
ZraI GACGTC 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.