RchiOBHm_Chr6g0308821

Mannose-P-dolichol utilization defect 1 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007362
Physical Location & Seq
Reverse (-)
66863189 .. 66866362
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27770

Sequence Viewer

Length: 699 bp
ATGAAAGTCCTCGGAATCGATTTCAGCTGTGCTCTGGGGGCTCTTAGCGACGGCCAGTTTCCCGAGAAGGATTGCTTGCTTCCCCTTATTTCCACGCTCCTCGGCTACGCCATCGTCGCCGCTTCCACCACCATATTAAAGATTTTGGAACATGGAAGTATCAGAGGTCTTAGTATCTTGTCTTTTGAGCTTGAACTAGTTGGTTACACGATTGCTTTGGCATATTGTCTTCATGGAGGGCTTCCCTTCTCAGCTTATGGGGAACTGGCCTTTCTTTTGATCCAAGCTCTAATATTAGTTGCCATTATGTACTATTACTCCCAACCTGTGAGCATCAAAAGATGGATGACGGCATTACTATATTGTGGTATAGCACCAACCATATTAGCAGGTCGAATTGATCCTGTTCTATTTGAAGCTCTATATGCATCACAACATGCAATTTTTCTCTTTGCCAAGATCCCGCAGATATGGCAGAACTTTTCTAACAAAAGTACAGGGGAGCTCAGCTTCTTAACGAACTTTATGAACTTTGGAGGTTCTATGGTGAGAGTTTTCACCAGCATCCAAGAACAAGCTCCGAAAAGTGTTTTGTTGGGCTCTGTACTTGGTGTTGCAACAAATTCCATCCTCCTGAGTCAGATACTTATATACCAAAAGCCAAAATCTCGTGCTCAGAAGGAGAAGAAAACGGAGTAG

Protein Analysis

232

Amino Acids

25.4

Weight (kDa)

8.61

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 146 - 196 4.5e-13 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AciI CCGC 2 cut(s) 120, 464
AclWI GGATC 3 cut(s) 274, 395, 454
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 1 cut(s) 622
AfaI GTAC 3 cut(s) 311, 496, 606
AgsI TTSAA 2 cut(s) 194, 416
AhlI ACTAGT 1 cut(s) 196
AluBI AGCT 8 cut(s) 27, 190, 254, 287, 419, 505, 510, 578
AluI AGCT 8 cut(s) 27, 190, 254, 287, 419, 505, 510, 578
Alw21I GWGCWC 3 cut(s) 34, 507, 676
AlwI GGATC 3 cut(s) 274, 395, 454
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 2 cut(s) 52, 267
ApoI RAATTY 1 cut(s) 622
AsuHPI GGTGA 2 cut(s) 550, 559
AvaI CYCGRG 1 cut(s) 62
BanII GRGCYC 3 cut(s) 43, 507, 602
BauI CACGAG 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 3 cut(s) 34, 507, 676
BccI CCATC 3 cut(s) 119, 336, 635
BceAI ACGGC 2 cut(s) 67, 366
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 1 cut(s) 197
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 506
BlsI GCNGC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 62
BmsI GCATC 3 cut(s) 342, 437, 573
BpiI GAAGAC 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 10, 100
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bse1I ACTGG 2 cut(s) 55, 270
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 2 cut(s) 10, 100
BseGI GGATG 3 cut(s) 351, 564, 627
BseMII CTCAG 4 cut(s) 264, 520, 626, 689
BseNI ACTGG 2 cut(s) 55, 270
BseRI GAGGAG 1 cut(s) 89
BshFI GGCC 2 cut(s) 54, 269
BshVI ATCGAT 1 cut(s) 18
BsiHKAI GWGCWC 3 cut(s) 34, 507, 676
BsiHKCI CYCGRG 1 cut(s) 62
BsnI GGCC 2 cut(s) 54, 269
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 5 cut(s) 34, 43, 507, 602, 676
Bsp143I GATC 3 cut(s) 279, 400, 459
Bsp1720I GCTNAGC 1 cut(s) 506
BspACI CCGC 2 cut(s) 120, 464
BspANI GGCC 2 cut(s) 54, 269
BspCNI CTCAG 4 cut(s) 263, 519, 627, 688
BspDI ATCGAT 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 274, 395, 454
BsrI ACTGG 2 cut(s) 55, 270
BssECI CCNNGG 2 cut(s) 10, 100
BssMI GATC 3 cut(s) 279, 400, 459
BssSI CACGAG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 669
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 6 cut(s) 44, 170, 250, 506, 635, 675
BstF5I GGATG 3 cut(s) 351, 564, 627
BstKTI GATC 3 cut(s) 282, 403, 462
BstMBI GATC 3 cut(s) 279, 400, 459
BstMWI GCNNNNNNNGC 4 cut(s) 38, 116, 425, 472
BstNSI RCATGY 1 cut(s) 440
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 18
BsuRI GGCC 2 cut(s) 54, 269
BsuTUI ATCGAT 1 cut(s) 18
BtsCI GGATG 3 cut(s) 351, 564, 627
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 18
Csp6I GTAC 3 cut(s) 310, 495, 605
CviAII CATG 3 cut(s) 152, 233, 437
CviQI GTAC 3 cut(s) 310, 495, 605
DdeI CTNAG 6 cut(s) 44, 170, 250, 506, 635, 675
DpnI GATC 3 cut(s) 281, 402, 461
DpnII GATC 3 cut(s) 279, 400, 459
EaeI YGGCCR 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 505
Eco24I GRGCYC 3 cut(s) 43, 507, 602
Eco53kI GAGCTC 1 cut(s) 505
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 505
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 3 cut(s) 43, 507, 602
FaeI CATG 3 cut(s) 155, 236, 440
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FatI CATG 3 cut(s) 151, 232, 436
FauI CCCGC 1 cut(s) 471
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 3 cut(s) 358, 551, 614
FriOI GRGCYC 3 cut(s) 43, 507, 602
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 197
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 2 cut(s) 54, 269
Hin1II CATG 3 cut(s) 155, 236, 440
HinfI GANTC 2 cut(s) 15, 637
HphI GGTGA 2 cut(s) 550, 559
Hpy188I TCNGA 5 cut(s) 14, 164, 582, 642, 678
Hpy188III TCNNGA 2 cut(s) 62, 634
Hpy99I CGWCG 2 cut(s) 53, 119
HpyAV CCTTC 3 cut(s) 61, 256, 673
HpyCH4V TGCA 3 cut(s) 428, 440, 617
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 116, 425, 472
HpyF3I CTNAG 6 cut(s) 44, 170, 250, 506, 635, 675
Hsp92II CATG 3 cut(s) 155, 236, 440
Kzo9I GATC 3 cut(s) 279, 400, 459
LmnI GCTCC 3 cut(s) 102, 502, 583
LpnPI CCDG 9 cut(s) 20, 68, 251, 339, 375, 417, 483, 574, 647
LweI GCATC 3 cut(s) 342, 437, 573
MaeI CTAG 1 cut(s) 197
MaeIII GTNAC 1 cut(s) 203
MalI GATC 3 cut(s) 281, 402, 461
MboI GATC 3 cut(s) 279, 400, 459
MboII GAAGA 2 cut(s) 221, 697
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 5 cut(s) 34, 43, 507, 602, 676
MluCI AATT 3 cut(s) 396, 441, 622
MlyI GAGTC 1 cut(s) 646
MnlI CCTC 6 cut(s) 20, 110, 158, 230, 530, 641
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 137, 515
MspA1I CMGCKG 1 cut(s) 27
MwoI GCNNNNNNNGC 4 cut(s) 38, 116, 425, 472
NdeII GATC 3 cut(s) 279, 400, 459
NlaIII CATG 3 cut(s) 155, 236, 440
NmeAIII GCCGAG 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 440
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 507
PsuI RGATCY 1 cut(s) 459
PvuII CAGCTG 1 cut(s) 27
RsaI GTAC 3 cut(s) 311, 496, 606
RsaNI GTAC 3 cut(s) 310, 495, 605
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 2 cut(s) 137, 515
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 3 cut(s) 279, 400, 459
SchI GAGTC 1 cut(s) 646
SduI GDGCHC 5 cut(s) 34, 43, 507, 602, 676
SfaNI GCATC 3 cut(s) 342, 437, 573
SpeI ACTAGT 1 cut(s) 196
Sse9I AATT 3 cut(s) 396, 441, 622
SsiI CCGC 2 cut(s) 120, 464
SspI AATATT 1 cut(s) 294
SspMI CTAG 1 cut(s) 197
SstI GAGCTC 1 cut(s) 507
TaqI TCGA 2 cut(s) 18, 394
TasI AATT 3 cut(s) 396, 441, 622
TatI WGTACW 3 cut(s) 309, 494, 604
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 2 cut(s) 137, 515
Tru9I TTAA 2 cut(s) 137, 515
TspDTI ATGAA 3 cut(s) 17, 221, 542
XapI RAATTY 1 cut(s) 622
XceI RCATGY 1 cut(s) 440
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.