Rroxscaffold_7G00159890

Mannose-P-dolichol utilization defect 1 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007362
Physical Location & Seq
Forward (+)
2926631 .. 2928675
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159890.1

Sequence Viewer

Length: 618 bp
ATGAAAGTCCTCGGAATCGATTTCACCTGTGCTTTAGGGGCTCTTAGCAACGGCCAGTTTCCCGAGAAGGATTGCTTGCTTCCACTTATTTCCACGCTCCTCGGCTACGCCATCGTCGCCGCTTCCACCACCATATTAAAGATTTTGGAACATGGAAGTATCAGAGGTCTTAGTATCTTGTCTTTTGAGCTTGAACTAGTTGGTTACACGATTGCTTTGGCATATTGTCTTCATGGAGGGCTTCCCTTCTCAGCTTATGGGGAACTGGCTTTTCTTTTGATCCAAGCTCTAATATTAGTTGCCATTATGTACTATTACTCCCAACCTGTGAGCATCAAAAGATGGATGGCGGCATTACTATATTGTGGTATAGCACCAACCATATTAGCAGGTCGAATTGATCCTGTTCTATTTGAAGCTCTATATGCATCACAACATGCAATTTTTCTCTTTGCCAAGATCCCGCAGATATGGCAGAACTTTTCTAACAAAAGTACAGGGGAGCTCAGCTTCTTAACGAACTTTATGAACTTTGGAGGTTCTATGGTGAGAGTTTTCACCAGCATCCAAGAACAAGCTCCAAAAAGTGGTATCCTAGACCCTTGGCTGTCACTTTAA

Protein Analysis

205

Amino Acids

22.44

Weight (kDa)

6.27

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 146 - 195 4.2e-13 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AccB7I CCANNNNNTGG 1 cut(s) 587
AciI CCGC 3 cut(s) 120, 350, 464
AclWI GGATC 3 cut(s) 274, 395, 454
AcoI YGGCCR 1 cut(s) 52
AfaI GTAC 2 cut(s) 311, 496
AfiI CCNNNNNNNGG 1 cut(s) 587
AgsI TTSAA 2 cut(s) 194, 416
AhlI ACTAGT 1 cut(s) 196
AluBI AGCT 7 cut(s) 190, 254, 287, 419, 505, 510, 578
AluI AGCT 7 cut(s) 190, 254, 287, 419, 505, 510, 578
Alw21I GWGCWC 1 cut(s) 507
AlwI GGATC 3 cut(s) 274, 395, 454
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 52
AsuHPI GGTGA 3 cut(s) 16, 550, 559
AvaI CYCGRG 1 cut(s) 62
BanII GRGCYC 2 cut(s) 43, 507
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 507
BccI CCATC 3 cut(s) 119, 336, 340
BceAI ACGGC 1 cut(s) 67
BciVI GTATCC 1 cut(s) 602
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 2 cut(s) 197, 596
BfuAI ACCTGC 1 cut(s) 380
BfuI GTATCC 1 cut(s) 602
BisI GCNGC 2 cut(s) 120, 351
BlpI GCTNAGC 1 cut(s) 506
BlsI GCNGC 2 cut(s) 121, 352
BmeT110I CYCGRG 1 cut(s) 62
BmsI GCATC 3 cut(s) 342, 437, 573
BpiI GAAGAC 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 18
BsaJI CCNNGG 3 cut(s) 10, 100, 602
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse1I ACTGG 2 cut(s) 55, 270
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 3 cut(s) 10, 100, 602
BseGI GGATG 2 cut(s) 351, 564
BseLI CCNNNNNNNGG 1 cut(s) 587
BseMII CTCAG 2 cut(s) 264, 520
BseNI ACTGG 2 cut(s) 55, 270
BseRI GAGGAG 1 cut(s) 89
BshFI GGCC 1 cut(s) 54
BshVI ATCGAT 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 507
BsiHKCI CYCGRG 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 587
BsnI GGCC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 43, 507
Bsp143I GATC 3 cut(s) 279, 400, 459
Bsp1720I GCTNAGC 1 cut(s) 506
BspACI CCGC 3 cut(s) 120, 350, 464
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 2 cut(s) 263, 519
BspDI ATCGAT 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 274, 395, 454
BsrI ACTGG 2 cut(s) 55, 270
BssECI CCNNGG 3 cut(s) 10, 100, 602
BssMI GATC 3 cut(s) 279, 400, 459
BssT1I CCWWGG 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 4 cut(s) 44, 170, 250, 506
BstF5I GGATG 2 cut(s) 351, 564
BstKTI GATC 3 cut(s) 282, 403, 462
BstMBI GATC 3 cut(s) 279, 400, 459
BstMWI GCNNNNNNNGC 4 cut(s) 38, 116, 425, 472
BstNSI RCATGY 1 cut(s) 440
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 18
BsuI GTATCC 1 cut(s) 602
BsuRI GGCC 1 cut(s) 54
BsuTUI ATCGAT 1 cut(s) 18
BtsCI GGATG 2 cut(s) 351, 564
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 18
Csp6I GTAC 2 cut(s) 310, 495
CviAII CATG 3 cut(s) 152, 233, 437
CviQI GTAC 2 cut(s) 310, 495
DdeI CTNAG 4 cut(s) 44, 170, 250, 506
DpnI GATC 3 cut(s) 281, 402, 461
DpnII GATC 3 cut(s) 279, 400, 459
EaeI YGGCCR 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 602
Eco24I GRGCYC 2 cut(s) 43, 507
Eco53kI GAGCTC 1 cut(s) 505
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 602
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 2 cut(s) 43, 507
ErhI CCWWGG 1 cut(s) 602
FaeI CATG 3 cut(s) 155, 236, 440
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FatI CATG 3 cut(s) 151, 232, 436
FauI CCCGC 1 cut(s) 471
Fnu4HI GCNGC 2 cut(s) 120, 351
FokI GGATG 2 cut(s) 358, 551
FriOI GRGCYC 2 cut(s) 43, 507
Fsp4HI GCNGC 2 cut(s) 120, 351
FspBI CTAG 2 cut(s) 197, 596
GluI GCNGC 2 cut(s) 120, 351
HaeIII GGCC 1 cut(s) 54
Hin1II CATG 3 cut(s) 155, 236, 440
HinfI GANTC 1 cut(s) 15
HphI GGTGA 3 cut(s) 16, 550, 559
Hpy188I TCNGA 2 cut(s) 14, 164
Hpy188III TCNNGA 1 cut(s) 62
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 61, 256
HpyCH4V TGCA 2 cut(s) 428, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 116, 425, 472
HpyF3I CTNAG 4 cut(s) 44, 170, 250, 506
Hsp92II CATG 3 cut(s) 155, 236, 440
Kzo9I GATC 3 cut(s) 279, 400, 459
LmnI GCTCC 3 cut(s) 102, 502, 583
LpnPI CCDG 8 cut(s) 40, 68, 251, 339, 375, 417, 483, 574
LweI GCATC 3 cut(s) 342, 437, 573
MaeI CTAG 2 cut(s) 197, 596
MaeIII GTNAC 2 cut(s) 203, 609
MalI GATC 3 cut(s) 281, 402, 461
MboI GATC 3 cut(s) 279, 400, 459
MboII GAAGA 1 cut(s) 221
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 43, 507
MluCI AATT 2 cut(s) 396, 441
MnlI CCTC 5 cut(s) 20, 110, 158, 230, 530
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 3 cut(s) 137, 515, 616
MwoI GCNNNNNNNGC 4 cut(s) 38, 116, 425, 472
NdeII GATC 3 cut(s) 279, 400, 459
NlaIII CATG 3 cut(s) 155, 236, 440
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 609
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 440
PfeI GAWTC 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 587
PkrI GCNGC 2 cut(s) 121, 352
Psp124BI GAGCTC 1 cut(s) 507
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 2 cut(s) 311, 496
RsaNI GTAC 2 cut(s) 310, 495
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 3 cut(s) 137, 515, 616
SatI GCNGC 2 cut(s) 120, 351
Sau3AI GATC 3 cut(s) 279, 400, 459
SduI GDGCHC 2 cut(s) 43, 507
SfaNI GCATC 3 cut(s) 342, 437, 573
SpeI ACTAGT 1 cut(s) 196
Sse9I AATT 2 cut(s) 396, 441
SsiI CCGC 3 cut(s) 120, 350, 464
SspI AATATT 1 cut(s) 294
SspMI CTAG 2 cut(s) 197, 596
SstI GAGCTC 1 cut(s) 507
StyI CCWWGG 1 cut(s) 602
TaqI TCGA 2 cut(s) 18, 394
TasI AATT 2 cut(s) 396, 441
TatI WGTACW 2 cut(s) 309, 494
TauI GCSGC 2 cut(s) 122, 353
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 3 cut(s) 137, 515, 616
Tru9I TTAA 3 cut(s) 137, 515, 616
TseFI GTSAC 1 cut(s) 609
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 3 cut(s) 17, 221, 542
Van91I CCANNNNNTGG 1 cut(s) 587
XceI RCATGY 1 cut(s) 440
XspI CTAG 2 cut(s) 197, 596
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.