RchiOBHm_Chr7g0177501

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010992
Physical Location & Seq
Forward (+)
436142 .. 438483
2342 bp
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UTR
Exon/CDS
Intron
PRQ15811

Sequence Viewer

Length: 498 bp
ATGGCGACGACCGTTGTGCTCCCAAGTGCTCCAAGGGCTTGGATTGATAGCAGAGAGACCCAATTTGCAGAGCACAAAGTGGGCTCTGTAAATTGTGCTTGGCAATGGCACCGCTGCCTCAGTTATAGGCTTTCGTTTTCTACTACACACAAAAAGAGGAGCTTTTTACCACTGGCGACTTCTTCTTCTTCGTCATCTTCGTTTTCTCCCAAGACCAAGCTCTACGTCAGTGGGCTTTCGTTTCGTACCACTGAAGAAAGTTTGCGAAATGCTTTCCAAAATTTTGGACATCTTGTAGAAGTCAATCTGGTGATGGACAGGATAGCAAATAGGCCGAGAGGATTTGCTTTCCTCCGCTATGAAACCGAGGAGGAATCTCAAAAGGCTATCGAGGGAATGCATGGAAAGTTTTTGGATGGCAGAGTAATATTTGTTGAAGAAGCAAAGCCCAGATCAGAGCTACGACAAACCCTCAAACAAAACCCAAGACAGTATTAA

Protein Analysis

165

Amino Acids

18.98

Weight (kDa)

9.82

Isoelectric Point (pI)

58.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 74 - 143 2.4e-19 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AciI CCGC 2 cut(s) 112, 355
AcsI RAATTY 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 79
AfaI GTAC 1 cut(s) 247
AgsI TTSAA 1 cut(s) 437
AluBI AGCT 3 cut(s) 162, 220, 460
AluI AGCT 3 cut(s) 162, 220, 460
Alw21I GWGCWC 3 cut(s) 21, 31, 75
Alw26I GTCTC 1 cut(s) 50
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 1 cut(s) 114
ApoI RAATTY 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 322
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 86
Bbv12I GWGCWC 3 cut(s) 21, 31, 75
BbvI GCAGC 1 cut(s) 101
BccI CCATC 2 cut(s) 307, 410
BcgI CGANNNNNNTGC 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 50
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 32, 366
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 110
BseDI CCNNGG 2 cut(s) 32, 366
BseGI GGATG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 133
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 2 cut(s) 172, 383
BseXI GCAGC 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 334
BshNI GGYRCC 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 21, 31, 75
BsmAI GTCTC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 1 cut(s) 334
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 4 cut(s) 21, 31, 75, 86
Bsp143I GATC 1 cut(s) 452
BspACI CCGC 2 cut(s) 112, 355
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 108
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 110
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 32, 366
BssMI GATC 1 cut(s) 452
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 13, 492
BstDEI CTNAG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 1 cut(s) 455
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 452
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 101
BstXI CCANNNNNNTGG 2 cut(s) 39, 284
BsuRI GGCC 1 cut(s) 334
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 3 cut(s) 170, 235, 249
Csp6I GTAC 1 cut(s) 246
CviAII CATG 1 cut(s) 401
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 1 cut(s) 119
DpnI GATC 1 cut(s) 454
DpnII GATC 1 cut(s) 452
DraIII CACNNNGTG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 86
Eco31I GGTCTC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 32
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 1 cut(s) 404
FaiI YATR 3 cut(s) 126, 360, 402
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FatI CATG 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 115
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 115
HaeIII GGCC 1 cut(s) 334
Hin1II CATG 1 cut(s) 404
HinfI GANTC 1 cut(s) 374
HphI GGTGA 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 457
Hpy99I CGWCG 1 cut(s) 10
HpyCH4III ACNGT 2 cut(s) 13, 492
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 2 cut(s) 68, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 1 cut(s) 119
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 1 cut(s) 404
Kzo9I GATC 1 cut(s) 452
LmnI GCTCC 3 cut(s) 24, 34, 159
LpnPI CCDG 4 cut(s) 158, 293, 304, 463
Lsp1109I GCAGC 1 cut(s) 101
MaeII ACGT 1 cut(s) 225
MalI GATC 1 cut(s) 454
MboI GATC 1 cut(s) 452
MboII GAAGA 6 cut(s) 174, 177, 180, 189, 266, 449
MhlI GDGCHC 4 cut(s) 21, 31, 75, 86
MluCI AATT 3 cut(s) 62, 91, 280
MnlI CCTC 8 cut(s) 128, 150, 332, 361, 362, 364, 385, 482
Mph1103I ATGCAT 1 cut(s) 402
MseI TTAA 1 cut(s) 496
MspA1I CMGCKG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 402
MwoI GCNNNNNNNGC 1 cut(s) 35
NdeII GATC 1 cut(s) 452
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 1 cut(s) 110
NmeAIII GCCGAG 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PctI GAATGC 1 cut(s) 402
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 110
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 1 cut(s) 452
SduI GDGCHC 4 cut(s) 21, 31, 75, 86
SetI ASST 4 cut(s) 164, 222, 228, 462
Sse9I AATT 3 cut(s) 62, 91, 280
SsiI CCGC 2 cut(s) 112, 355
SspI AATATT 1 cut(s) 429
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 13, 492
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 390
TasI AATT 3 cut(s) 62, 91, 280
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TscAI CASTG 3 cut(s) 177, 235, 256
TseI GCWGC 1 cut(s) 114
TspDTI ATGAA 1 cut(s) 375
TspRI CASTG 3 cut(s) 177, 235, 256
XapI RAATTY 1 cut(s) 280
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.