Rh7BG006000

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010992
Physical Location & Seq
Forward (+)
370831 .. 372874
2044 bp
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UTR
Exon/CDS
Intron
Rh7BG006000.1

Sequence Viewer

Length: 495 bp
ATGGCGACGACCGTTGTGCTCACAAGTGCTCCAAGGGCTTGGGTTGATAGCAGAGAGACCCAATTTGCAGAGCACAAAAAAGTGGGCTCTGTAAATTGTGCTTGGCAATGGCAGCGCTGCCTCAGTTATAGGCTTTCGTTTTCTACTACACAGAAAAAGAGGAGGAGCTTTTTACCACTGGCGACTTCTTCATCTTCGTTTTCTCCCAAGACCAAGCTCTACGTCAGTGGGCTTTCATTTCGTACCACTGAAGAGAGTTTGCGAAATGCTTTCCACAATTTTGGACAACTTGTGGAAGTCAATCTGGTGATGGACAGGATAGCAAATAGGCCGAGAGGATTTGCTTTCCTCCGCTATGAAACCGAGGAGGAATCTCAAAAGGCTATCGAGGGAATGCATGGAAAGTTTTTGGATGGCAGAGTAATATTTGTTGAAGAAGCAAAGCCCAGATCAGAGCTACGACAAACCCTCAAACAAAACCCAAGACAGTATTAA

Protein Analysis

164

Amino Acids

18.97

Weight (kDa)

10.0

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 73 - 142 3.4e-19 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 352
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 244
AfeI AGCGCT 1 cut(s) 116
AgsI TTSAA 1 cut(s) 434
AluBI AGCT 3 cut(s) 168, 217, 457
AluI AGCT 3 cut(s) 168, 217, 457
Alw21I GWGCWC 3 cut(s) 21, 31, 75
Alw26I GTCTC 1 cut(s) 50
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 112, 117
AspLEI GCGC 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 319
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 3 cut(s) 21, 31, 75
BbvI GCAGC 2 cut(s) 104, 124
BccI CCATC 2 cut(s) 304, 407
BcgI CGANNNNNNTGC 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 50
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 2 cut(s) 113, 118
BlsI GCNGC 2 cut(s) 114, 119
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 32, 363
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bse1I ACTGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 113
BseDI CCNNGG 2 cut(s) 32, 363
BseGI GGATG 1 cut(s) 418
BseMI GCAATG 1 cut(s) 113
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 3 cut(s) 175, 178, 380
BseXI GCAGC 2 cut(s) 104, 124
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 21, 31, 75
BsmAI GTCTC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 1 cut(s) 331
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 4 cut(s) 21, 31, 75, 89
Bsp143I GATC 1 cut(s) 449
BspACI CCGC 1 cut(s) 352
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 1 cut(s) 135
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 113
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 32, 363
BssMI GATC 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 13, 489
Bst6I CTCTTC 1 cut(s) 246
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 418
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 452
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 449
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 35, 112
BstV1I GCAGC 2 cut(s) 104, 124
BstXI CCANNNNNNTGG 2 cut(s) 39, 281
BsuRI GGCC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 418
BtsIMutI CAGTG 3 cut(s) 176, 232, 246
CfoI GCGC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 243
CviAII CATG 1 cut(s) 398
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 1 cut(s) 122
DpnI GATC 1 cut(s) 451
DpnII GATC 1 cut(s) 449
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 50
Eco47III AGCGCT 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 32
EcoT22I ATGCAT 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 1 cut(s) 401
FaiI YATR 3 cut(s) 129, 357, 399
FatI CATG 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 113, 118
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 2 cut(s) 113, 118
GlaI GCGC 1 cut(s) 116
GluI GCNGC 2 cut(s) 113, 118
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 331
HhaI GCGC 1 cut(s) 117
Hin1II CATG 1 cut(s) 401
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 1 cut(s) 371
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 454
Hpy99I CGWCG 1 cut(s) 10
HpyCH4III ACNGT 2 cut(s) 13, 489
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 68, 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 112
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 401
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 449
LmnI GCTCC 2 cut(s) 34, 165
LpnPI CCDG 4 cut(s) 164, 290, 301, 460
Lsp1109I GCAGC 2 cut(s) 104, 124
MaeII ACGT 1 cut(s) 222
MalI GATC 1 cut(s) 451
MboI GATC 1 cut(s) 449
MboII GAAGA 4 cut(s) 180, 186, 263, 446
MhlI GDGCHC 4 cut(s) 21, 31, 75, 89
MluCI AATT 3 cut(s) 62, 94, 277
MnlI CCTC 9 cut(s) 131, 153, 156, 329, 358, 359, 361, 382, 479
Mph1103I ATGCAT 1 cut(s) 399
MseI TTAA 1 cut(s) 493
Mva1269I GAATGC 1 cut(s) 399
MwoI GCNNNNNNNGC 2 cut(s) 35, 112
NdeII GATC 1 cut(s) 449
NlaIII CATG 1 cut(s) 401
NmeAIII GCCGAG 1 cut(s) 357
NsiI ATGCAT 1 cut(s) 399
PctI GAATGC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 2 cut(s) 114, 119
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
SaqAI TTAA 1 cut(s) 493
SatI GCNGC 2 cut(s) 113, 118
Sau3AI GATC 1 cut(s) 449
SduI GDGCHC 4 cut(s) 21, 31, 75, 89
SetI ASST 4 cut(s) 170, 219, 225, 459
Sse9I AATT 3 cut(s) 62, 94, 277
SsiI CCGC 1 cut(s) 352
SspI AATATT 1 cut(s) 426
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 13, 489
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 387
TasI AATT 3 cut(s) 62, 94, 277
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 493
Tru9I TTAA 1 cut(s) 493
TscAI CASTG 3 cut(s) 183, 232, 253
TseI GCWGC 2 cut(s) 112, 117
TspDTI ATGAA 3 cut(s) 180, 225, 372
TspRI CASTG 3 cut(s) 183, 232, 253
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.