RchiOBHm_Chr7g0217651

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010629
Physical Location & Seq
Reverse (-)
35572532 .. 35573585
1054 bp
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UTR
Exon/CDS
Intron
PRQ19478

Sequence Viewer

Length: 444 bp
ATGGGAGTACCCAAAGTACGGAGGAGAGCAGCAGCAATGCAAATAATACTTCCAGTTACGGACTTAATACACATACCAGATCTCCATTTACTGAAGGAGGATGATTTGAAACTCATGGGACAGTTTATAAAGGACTACAGAGTACCTCCTGAGATCTGGTTCTCACTTCTGACAAGAATCAGATATTCTCGTGCCTTCCGTTCACCAAGAACCTGCAAGCTTGGCCTTCTTGCATTTACTGTGCTTGTTCATACTAGTGATGCTCCTGAAGAACTTCTGTCTTTCTTTGCTAATGAGCCAGACTATATTAATAGATCAATTAGAATTGTGCCATCAGAAGTAGTTTCTGGAACCATCAGAACAAATACAATGCCCTTATTGAAGATCATGCACCAACCCTCTTGTCCACTGCATCACATTGTTAGTGTTTCTGCTTCTACATAA

Protein Analysis

147

Amino Acids

16.73

Weight (kDa)

9.56

Isoelectric Point (pI)

56.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 22 - 128 3.2e-09 Domain of Unknown Function (DUF908)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
Acc36I ACCTGC 1 cut(s) 221
AcuI CTGAAG 2 cut(s) 113, 288
AfaI GTAC 3 cut(s) 9, 18, 144
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 2 cut(s) 109, 382
AhlI ACTAGT 1 cut(s) 254
AluBI AGCT 1 cut(s) 220
AluI AGCT 1 cut(s) 220
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 2 cut(s) 29, 32
AseI ATTAAT 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 195
BauI CACGAG 1 cut(s) 189
BbvI GCAGC 2 cut(s) 41, 44
BccI CCATC 2 cut(s) 340, 362
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 221
BglII AGATCT 2 cut(s) 79, 153
BisI GCNGC 2 cut(s) 30, 33
BlsI GCNGC 2 cut(s) 31, 34
BmiI GGNNCC 1 cut(s) 352
BmsI GCATC 2 cut(s) 250, 421
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 42
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 37
BseXI GCAGC 2 cut(s) 41, 44
BshFI GGCC 1 cut(s) 225
BslFI GGGAC 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 132
BsnI GGCC 1 cut(s) 225
Bsp143I GATC 4 cut(s) 79, 153, 314, 384
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 352
BspMI ACCTGC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 1 cut(s) 53
BssMI GATC 4 cut(s) 79, 153, 314, 384
BssSI CACGAG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 123, 241
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 4 cut(s) 82, 156, 317, 387
BstMBI GATC 4 cut(s) 79, 153, 314, 384
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstSFI CTRYAG 1 cut(s) 136
BstV1I GCAGC 2 cut(s) 41, 44
BstX2I RGATCY 2 cut(s) 79, 153
BstYI RGATCY 2 cut(s) 79, 153
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 407
BtsIMutI CAGTG 1 cut(s) 407
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 8, 17, 143
CviAII CATG 2 cut(s) 115, 388
CviJI RGCY 3 cut(s) 220, 225, 298
CviKI_1 RGCY 3 cut(s) 220, 225, 298
CviQI GTAC 3 cut(s) 8, 17, 143
DdeI CTNAG 1 cut(s) 150
DpnI GATC 4 cut(s) 81, 155, 316, 386
DpnII GATC 4 cut(s) 79, 153, 314, 384
Eco57I CTGAAG 2 cut(s) 113, 288
FaeI CATG 2 cut(s) 118, 391
FaiI YATR 7 cut(s) 74, 116, 128, 252, 306, 389, 442
FaqI GGGAC 1 cut(s) 132
FatI CATG 2 cut(s) 114, 387
Fnu4HI GCNGC 2 cut(s) 30, 33
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 2 cut(s) 30, 33
FspBI CTAG 1 cut(s) 255
GluI GCNGC 2 cut(s) 30, 33
HaeIII GGCC 1 cut(s) 225
Hin1II CATG 2 cut(s) 118, 391
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 1 cut(s) 177
HphI GGTGA 1 cut(s) 195
Hpy166II GTNNAC 2 cut(s) 203, 407
Hpy188I TCNGA 4 cut(s) 171, 182, 337, 359
Hpy188III TCNNGA 3 cut(s) 149, 266, 348
Hpy8I GTNNAC 2 cut(s) 203, 407
HpyAV CCTTC 3 cut(s) 88, 205, 236
HpyCH4III ACNGT 2 cut(s) 123, 241
HpyCH4V TGCA 5 cut(s) 40, 216, 233, 391, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 2 cut(s) 118, 391
Kzo9I GATC 4 cut(s) 79, 153, 314, 384
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 8 cut(s) 66, 90, 142, 162, 226, 279, 312, 333
Lsp1109I GCAGC 2 cut(s) 41, 44
LweI GCATC 2 cut(s) 250, 421
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 55
MalI GATC 4 cut(s) 81, 155, 316, 386
MboI GATC 4 cut(s) 79, 153, 314, 384
MboII GAAGA 2 cut(s) 281, 394
MflI RGATCY 2 cut(s) 79, 153
MluCI AATT 2 cut(s) 318, 324
MnlI CCTC 4 cut(s) 15, 91, 156, 409
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 2 cut(s) 65, 309
MslI CAYNNNNRTG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 222
NdeII GATC 4 cut(s) 79, 153, 314, 384
NlaIII CATG 2 cut(s) 118, 391
NlaIV GGNNCC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 177
PkrI GCNGC 2 cut(s) 31, 34
PshBI ATTAAT 1 cut(s) 309
PsiI TTATAA 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 352
PsuI RGATCY 2 cut(s) 79, 153
RsaI GTAC 3 cut(s) 9, 18, 144
RsaNI GTAC 3 cut(s) 8, 17, 143
RseI CAYNNNNRTG 1 cut(s) 255
SaqAI TTAA 2 cut(s) 65, 309
SatI GCNGC 2 cut(s) 30, 33
Sau3AI GATC 4 cut(s) 79, 153, 314, 384
SetI ASST 3 cut(s) 148, 215, 222
SfaNI GCATC 2 cut(s) 250, 421
SfcI CTRYAG 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 255
SpeI ACTAGT 1 cut(s) 254
Sse9I AATT 2 cut(s) 318, 324
SspMI CTAG 1 cut(s) 255
TaaI ACNGT 2 cut(s) 123, 241
TasI AATT 2 cut(s) 318, 324
TfiI GAWTC 1 cut(s) 177
Tru1I TTAA 2 cut(s) 65, 309
Tru9I TTAA 2 cut(s) 65, 309
TscAI CASTG 1 cut(s) 414
TseI GCWGC 2 cut(s) 29, 32
TspDTI ATGAA 1 cut(s) 239
TspGWI ACGGA 3 cut(s) 34, 74, 188
TspRI CASTG 1 cut(s) 414
VspI ATTAAT 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.