RLG00000002136

HAUS augmin-like complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014661
Physical Location & Seq
Forward (+)
26105723 .. 26107255
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002136

Sequence Viewer

Length: 570 bp
ATGACAGAGGCTGCATGTTGTACGAATGTGATGAATAGCAGAATCAAAGCACTAGTGAAAGCCATTCACTCTACTCCCACGCAGTCCGTTCTCTATCTCTCCGGCAGAGCCTTTCAGATTCTGTCAAAATTGACTAAGACCGGTGATTTCAGGCGGTGGCGTGGTTACTCTCCGTTCCAAGAGCCATTCCGGACAAGTTTTGTAGCCAGCACACTGTTGAAGAAATGGCTCTGTTGGCTTACAATCGCGCTCTCAAGCTCTCCTCACCAGGGGGAAGATTTGGACTTTGCTTATGATAGCATTTCTGCCTTTTCTTCCAGCCAAGACAACCAGGAGGTAGTTTTCGCAGCTGAAGAGGGACTCAAAGATATTAGGGATGCAACTATGCCATATAAATCTGAAGCTTTACAGTTGCAGAAGCAACTTAGACGTCTGCACTCTCAATTTGACATGCGTACTGGTCAAGCTTCTTCTTTGATCCAAGGTAGACAAGTTCGAGTTGCTGCAACATCTACTATTAATGGACATCTTATAACCATAGATGACACCCTTTTTGCAAAAAATTTGTAG

Protein Analysis

190

Amino Acids

21.21

Weight (kDa)

9.24

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS-augmin3 PF14932 90 - 188 2.1e-14 HAUS augmin-like complex subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AatII GACGTC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 487
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 1 cut(s) 154
AclWI GGATC 1 cut(s) 472
AcsI RAATTY 1 cut(s) 562
AcuI CTGAAG 2 cut(s) 372, 420
AcyI GRCGYC 1 cut(s) 430
AfaI GTAC 2 cut(s) 22, 457
AfiI CCNNNNNNNGG 1 cut(s) 269
AgeI ACCGGT 1 cut(s) 140
AgsI TTSAA 1 cut(s) 220
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 2 cut(s) 267, 330
AluBI AGCT 4 cut(s) 258, 350, 404, 467
AluI AGCT 4 cut(s) 258, 350, 404, 467
AlwI GGATC 1 cut(s) 472
AlwNI CAGNNNCTG 2 cut(s) 11, 121
Aor13HI TCCGGA 1 cut(s) 189
ApeKI GCWGC 3 cut(s) 11, 347, 503
ApoI RAATTY 1 cut(s) 562
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AseI ATTAAT 1 cut(s) 519
AsiGI ACCGGT 1 cut(s) 140
AspLEI GCGC 1 cut(s) 250
AsuHPI GGTGA 2 cut(s) 155, 257
BbvI GCAGC 2 cut(s) 359, 490
BciT130I CCWGG 2 cut(s) 269, 332
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 1 cut(s) 53
BisI GCNGC 3 cut(s) 12, 348, 504
BlsI GCNGC 3 cut(s) 13, 349, 505
Bme1390I CCNGG 2 cut(s) 269, 332
BmrFI CCNGG 2 cut(s) 269, 332
BmsI GCATC 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 2 cut(s) 268, 481
BsaWI WCCGGW 2 cut(s) 140, 189
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 463
BseAI TCCGGA 1 cut(s) 189
BseBI CCWGG 2 cut(s) 269, 332
BseDI CCNNGG 2 cut(s) 268, 481
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 252
BseXI GCAGC 2 cut(s) 359, 490
BsgI GTGCAG 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 248
BshTI ACCGGT 1 cut(s) 140
BsiSI CCGG 3 cut(s) 102, 141, 190
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 372
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 1 cut(s) 477
BspACI CCGC 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 189
BspFNI CGCG 1 cut(s) 248
BspPI GGATC 1 cut(s) 472
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 463
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 268, 481
BssMI GATC 1 cut(s) 477
BssNI GRCGYC 1 cut(s) 430
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 2 cut(s) 269, 332
Bst4CI ACNGT 2 cut(s) 216, 411
Bst6I CTCTTC 1 cut(s) 348
BstACI GRCGYC 1 cut(s) 430
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 135, 425
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 1 cut(s) 480
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNI CCWGG 2 cut(s) 269, 332
BstNSI RCATGY 2 cut(s) 18, 454
BstSCI CCNGG 2 cut(s) 267, 330
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 359, 490
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 212
Cac8I GCNNGC 1 cut(s) 208
CaiI CAGNNNCTG 2 cut(s) 11, 121
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 140
Csp6I GTAC 2 cut(s) 21, 456
CspAI ACCGGT 1 cut(s) 140
CviAII CATG 2 cut(s) 15, 451
CviQI GTAC 2 cut(s) 21, 456
DdeI CTNAG 2 cut(s) 135, 425
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 481
Eco57I CTGAAG 2 cut(s) 372, 420
EcoRII CCWGG 2 cut(s) 267, 330
EcoT14I CCWWGG 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 2 cut(s) 18, 454
FaiI YATR 8 cut(s) 16, 294, 386, 391, 393, 452, 533, 539
FaqI GGGAC 1 cut(s) 372
FatI CATG 2 cut(s) 14, 450
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 3 cut(s) 12, 348, 504
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 3 cut(s) 12, 348, 504
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 249
GluI GCNGC 3 cut(s) 12, 348, 504
HapII CCGG 3 cut(s) 102, 141, 190
HhaI GCGC 1 cut(s) 250
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 2 cut(s) 18, 454
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HindIII AAGCTT 2 cut(s) 402, 465
HinfI GANTC 3 cut(s) 42, 118, 360
HpaII CCGG 3 cut(s) 102, 141, 190
HphI GGTGA 2 cut(s) 155, 257
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 2 cut(s) 117, 400
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 1 cut(s) 488
HpyCH4III ACNGT 2 cut(s) 216, 411
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 6 cut(s) 14, 380, 415, 436, 506, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 2 cut(s) 135, 425
HpySE526I ACGT 1 cut(s) 430
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 2 cut(s) 18, 454
HspAI GCGC 1 cut(s) 248
Kpn2I TCCGGA 1 cut(s) 189
Kzo9I GATC 1 cut(s) 477
Lsp1109I GCAGC 2 cut(s) 359, 490
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 479
MboI GATC 1 cut(s) 477
MboII GAAGA 5 cut(s) 232, 287, 306, 365, 462
MluCI AATT 3 cut(s) 128, 443, 562
MlyI GAGTC 1 cut(s) 354
MnlI CCTC 3 cut(s) 273, 328, 349
MroI TCCGGA 1 cut(s) 189
MseI TTAA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 3 cut(s) 102, 141, 190
MspR9I CCNGG 2 cut(s) 269, 332
MvaI CCWGG 2 cut(s) 269, 332
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 1 cut(s) 477
NlaIII CATG 2 cut(s) 18, 454
NspI RCATGY 2 cut(s) 18, 454
PfeI GAWTC 2 cut(s) 42, 118
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 3 cut(s) 13, 349, 505
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PshBI ATTAAT 1 cut(s) 519
PsiI TTATAA 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 267, 330
PspGI CCWGG 2 cut(s) 267, 330
PstNI CAGNNNCTG 2 cut(s) 11, 121
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 22, 457
RsaNI GTAC 2 cut(s) 21, 456
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 3 cut(s) 12, 348, 504
Sau3AI GATC 1 cut(s) 477
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 2 cut(s) 269, 332
SetI ASST 7 cut(s) 260, 339, 352, 406, 433, 469, 487
SfaNI GCATC 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 3 cut(s) 128, 443, 562
SsiI CCGC 1 cut(s) 154
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 2 cut(s) 267, 330
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 2 cut(s) 216, 411
TaiI ACGT 1 cut(s) 433
TaqI TCGA 1 cut(s) 496
TasI AATT 3 cut(s) 128, 443, 562
TfiI GAWTC 2 cut(s) 42, 118
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 1 cut(s) 219
TseI GCWGC 3 cut(s) 11, 347, 503
TspDTI ATGAA 1 cut(s) 47
TspGWI ACGGA 2 cut(s) 76, 162
TspRI CASTG 1 cut(s) 219
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 1 cut(s) 562
XceI RCATGY 2 cut(s) 18, 454
XmiI GTMKAC 1 cut(s) 487
XspI CTAG 1 cut(s) 53
ZraI GACGTC 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.