RLG00000019143

HAUS augmin-like complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014661
Physical Location & Seq
Reverse (-)
47281881 .. 47284908
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019143

Sequence Viewer

Length: 729 bp
ATGACAGAGGCCGCTTGTTGTACGAATGTGATGAATAGCGGAATTAGAGCACTAGTGAAAGCCATTCACTTTACTCCCACGCAGTCTGTTCTCCATCTCTCCGGTAGAGCCTCTCAGATTCCGTCAAAATTGACCAAGACCGGTGATTTCAGGCTGTGGAGTGGTTGCTCTTCTTTCCCGGAGCCGCAAGTACAGACTTGGAAACTATTCCGGACAAGTTTTGTAGCCAACATACTGTTGAAGAAATGGCTTTGTTGGCTTACAAACTCCATGTCTGCCTTTTCTTACTACCGAGACAACCAGGAGGCGGTTTTCGCAGCTGAAGAGGGACTCAAAGATATTCGGGATGCAACTCTGGCATATAGATCTGAAGTTTTACAGTTGCAGAAGCAGCTTAGACGTCTGCACTCTCAATTTGACATGCTTATTGGTCAAGCTTCTTCTTTGATCCAAGGTAGACAAGCTCGAGTTGCTGCAACATCTACTATTAATGGACATCTTACAACCATAGATGACACCCTTTCAGCAAAAAATTTGCAGTTCTTGGAAGGATTGCGTCTACAGCACAGGAATTGGCTCATTATCACTTTGGGGATGATTGGTGTTGACCCAAAAGATCTCGAAAAGGTGTTTATACAGCTTACACTTGAGTTCCTGTCAAAGCAGGTCCAGGATTTATCTGTGAACCTTATGCACCTCGTGAAAAAGTTCCTGTTTTCCGGAGGTTAG

Protein Analysis

243

Amino Acids

27.31

Weight (kDa)

9.48

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS-augmin3 PF14932 89 - 185 1.3e-13 HAUS augmin-like complex subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 403
Acc36I ACCTGC 1 cut(s) 655
AccI GTMKAC 2 cut(s) 457, 559
AccIII TCCGGA 2 cut(s) 210, 719
AciI CCGC 4 cut(s) 12, 39, 185, 308
AclWI GGATC 1 cut(s) 442
AcsI RAATTY 1 cut(s) 532
AcuI CTGAAG 2 cut(s) 342, 390
AcyI GRCGYC 1 cut(s) 400
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 2 cut(s) 22, 192
AfiI CCNNNNNNNGG 1 cut(s) 307
AgeI ACCGGT 1 cut(s) 140
AgsI TTSAA 1 cut(s) 241
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 2 cut(s) 300, 669
AluBI AGCT 5 cut(s) 320, 394, 437, 464, 640
AluI AGCT 5 cut(s) 320, 394, 437, 464, 640
Alw21I GWGCWC 1 cut(s) 52
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 465
Aor13HI TCCGGA 2 cut(s) 210, 719
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 3 cut(s) 317, 391, 473
ApoI RAATTY 1 cut(s) 532
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AseI ATTAAT 1 cut(s) 489
AsiGI ACCGGT 1 cut(s) 140
Asp700I GAANNNNTTC 2 cut(s) 206, 707
AspS9I GGNCC 1 cut(s) 667
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 465
AvaII GGWCC 1 cut(s) 667
BauI CACGAG 1 cut(s) 698
Bbv12I GWGCWC 1 cut(s) 52
BbvI GCAGC 3 cut(s) 329, 403, 460
BccI CCATC 1 cut(s) 102
BciT130I CCWGG 2 cut(s) 302, 671
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 655
BglII AGATCT 2 cut(s) 365, 616
BisI GCNGC 5 cut(s) 12, 185, 318, 392, 474
BlsI GCNGC 5 cut(s) 13, 186, 319, 393, 475
Bme1390I CCNGG 3 cut(s) 179, 302, 671
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 3 cut(s) 179, 302, 671
BmsI GCATC 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 4 cut(s) 101, 140, 210, 719
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse118I RCCGGY 1 cut(s) 140
BseAI TCCGGA 2 cut(s) 210, 719
BseBI CCWGG 2 cut(s) 302, 671
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 2 cut(s) 352, 600
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 329, 403, 460
BsgI GTGCAG 1 cut(s) 389
BshFI GGCC 1 cut(s) 11
BshTI ACCGGT 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 52
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 5 cut(s) 102, 141, 179, 211, 720
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 342
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 52
Bsp13I TCCGGA 2 cut(s) 210, 719
Bsp143I GATC 3 cut(s) 365, 447, 616
BspACI CCGC 4 cut(s) 12, 39, 185, 308
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 2 cut(s) 210, 719
BspLI GGNNCC 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 655
BspPI GGATC 1 cut(s) 442
BspQI GCTCTTC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 3 cut(s) 365, 447, 616
BssNI GRCGYC 1 cut(s) 400
BssSI CACGAG 1 cut(s) 698
BssT1I CCWWGG 1 cut(s) 451
Bst2BI CACGAG 1 cut(s) 698
Bst2UI CCWGG 2 cut(s) 302, 671
Bst4CI ACNGT 2 cut(s) 237, 381
Bst6I CTCTTC 2 cut(s) 175, 318
BstACI GRCGYC 1 cut(s) 400
BstDEI CTNAG 2 cut(s) 114, 395
BstF5I GGATG 2 cut(s) 352, 600
BstKTI GATC 3 cut(s) 368, 450, 619
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 3 cut(s) 365, 447, 616
BstMWI GCNNNNNNNGC 6 cut(s) 256, 314, 356, 391, 470, 562
BstNI CCWGG 2 cut(s) 302, 671
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 177, 300, 669
BstSFI CTRYAG 1 cut(s) 560
BstV1I GCAGC 3 cut(s) 329, 403, 460
BstX2I RGATCY 2 cut(s) 365, 616
BstYI RGATCY 2 cut(s) 365, 616
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 2 cut(s) 352, 600
BveI ACCTGC 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 667
CseI GACGC 1 cut(s) 545
Csp6I GTAC 2 cut(s) 21, 191
CspAI ACCGGT 1 cut(s) 140
CviAII CATG 2 cut(s) 271, 421
CviQI GTAC 2 cut(s) 21, 191
DdeI CTNAG 2 cut(s) 114, 395
DpnI GATC 3 cut(s) 367, 449, 618
DpnII GATC 3 cut(s) 365, 447, 616
DraIII CACNNNGTG 1 cut(s) 700
Eam1104I CTCTTC 2 cut(s) 175, 318
EarI CTCTTC 2 cut(s) 175, 318
Eco130I CCWWGG 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 667
Eco57I CTGAAG 2 cut(s) 342, 390
Eco88I CYCGRG 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 300, 669
EcoT14I CCWWGG 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 2 cut(s) 274, 424
FaiI YATR 8 cut(s) 233, 272, 361, 363, 422, 509, 635, 692
FaqI GGGAC 1 cut(s) 342
FatI CATG 2 cut(s) 270, 420
FblI GTMKAC 2 cut(s) 457, 559
Fnu4HI GCNGC 5 cut(s) 12, 185, 318, 392, 474
FokI GGATG 2 cut(s) 359, 607
Fsp4HI GCNGC 5 cut(s) 12, 185, 318, 392, 474
FspBI CTAG 1 cut(s) 53
GluI GCNGC 5 cut(s) 12, 185, 318, 392, 474
HaeIII GGCC 1 cut(s) 11
HapII CCGG 5 cut(s) 102, 141, 179, 211, 720
HgaI GACGC 1 cut(s) 545
Hin1I GRCGYC 1 cut(s) 400
Hin1II CATG 2 cut(s) 274, 424
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 2 cut(s) 118, 330
HpaII CCGG 5 cut(s) 102, 141, 179, 211, 720
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 4 cut(s) 458, 560, 607, 685
Hpy188I TCNGA 2 cut(s) 117, 370
Hpy188III TCNNGA 5 cut(s) 211, 344, 620, 700, 720
Hpy8I GTNNAC 4 cut(s) 458, 560, 607, 685
HpyAV CCTTC 1 cut(s) 542
HpyCH4III ACNGT 2 cut(s) 237, 381
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 6 cut(s) 350, 385, 406, 476, 538, 694
HpyF10VI GCNNNNNNNGC 6 cut(s) 256, 314, 356, 391, 470, 562
HpyF3I CTNAG 2 cut(s) 114, 395
HpySE526I ACGT 1 cut(s) 400
Hsp92I GRCGYC 1 cut(s) 400
Hsp92II CATG 2 cut(s) 274, 424
Kpn2I TCCGGA 2 cut(s) 210, 719
Kzo9I GATC 3 cut(s) 365, 447, 616
LguI GCTCTTC 1 cut(s) 175
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 3 cut(s) 329, 403, 460
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 400
MalI GATC 3 cut(s) 367, 449, 618
MboI GATC 3 cut(s) 365, 447, 616
MboII GAAGA 4 cut(s) 162, 253, 335, 432
MflI RGATCY 2 cut(s) 365, 616
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 5 cut(s) 42, 128, 413, 532, 571
MlyI GAGTC 1 cut(s) 324
MnlI CCTC 5 cut(s) 121, 298, 319, 707, 716
MroI TCCGGA 2 cut(s) 210, 719
MroXI GAANNNNTTC 2 cut(s) 206, 707
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 5 cut(s) 102, 141, 179, 211, 720
MspR9I CCNGG 3 cut(s) 179, 302, 671
MvaI CCWGG 2 cut(s) 302, 671
MwoI GCNNNNNNNGC 6 cut(s) 256, 314, 356, 391, 470, 562
NciI CCSGG 1 cut(s) 179
NdeII GATC 3 cut(s) 365, 447, 616
NlaIII CATG 2 cut(s) 274, 424
NlaIV GGNNCC 1 cut(s) 183
NspI RCATGY 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 175
PdmI GAANNNNTTC 2 cut(s) 206, 707
PfeI GAWTC 1 cut(s) 118
PfoI TCCNGGA 2 cut(s) 177, 669
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 5 cut(s) 13, 186, 319, 393, 475
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
PshBI ATTAAT 1 cut(s) 489
Psp6I CCWGG 2 cut(s) 300, 669
PspGI CCWGG 2 cut(s) 300, 669
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 667
PspXI VCTCGAGB 1 cut(s) 465
PsuI RGATCY 2 cut(s) 365, 616
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 2 cut(s) 22, 192
RsaNI GTAC 2 cut(s) 21, 191
SapI GCTCTTC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 12, 185, 318, 392, 474
Sau3AI GATC 3 cut(s) 365, 447, 616
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 3 cut(s) 179, 302, 671
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 560
Sfr274I CTCGAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 667
SlaI CTCGAG 1 cut(s) 465
SmlI CTYRAG 2 cut(s) 465, 647
SmoI CTYRAG 2 cut(s) 465, 647
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 5 cut(s) 42, 128, 413, 532, 571
SsiI CCGC 4 cut(s) 12, 39, 185, 308
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 3 cut(s) 177, 300, 669
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 237, 381
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 466, 621
TasI AATT 5 cut(s) 42, 128, 413, 532, 571
TatI WGTACW 1 cut(s) 190
TauI GCSGC 2 cut(s) 14, 187
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseI GCWGC 3 cut(s) 317, 391, 473
TspDTI ATGAA 1 cut(s) 47
TspGWI ACGGA 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 667
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 1 cut(s) 532
XceI RCATGY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 465
XmiI GTMKAC 2 cut(s) 457, 559
XmnI GAANNNNTTC 2 cut(s) 206, 707
XspI CTAG 1 cut(s) 53
ZraI GACGTC 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.