Rh5CG552700

HAUS augmin-like complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014661
Physical Location & Seq
Forward (+)
76918691 .. 76920830
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG552700.1

Sequence Viewer

Length: 606 bp
ATGAGAATGTTCTTCTCCGGCGATTCGTCGAATCGGAAGCACGTGGATTTAGGGGTACAGAGTACAAAGGAGAGGGATAAGCAGAAGCTTCTGGAGCAAACGAGGTTGGAGATTTCGGACAAGTTTTGTAGCCAACACACTATTGAAGAAATGGCTATGTTGGCTTACAATTGCGCTCTCAAGCTCTCCTCACCAGGGGAAGATTTGGACTTTGCTTATGATAGCATTTCTGCCTTTTCTTCCAGCAGAGACAACCAGGAGGCGGTTTTCGCAGCTGAAGAGAGACTCAAAGATATTCGGGATGCAACTCTGGCATATAAATCTGAAGCTTTACAGTTGCAGAAGCAGCTTAGAAGTCTGCACTCTCAATTTGACATGCTTACTGGTCAAGCTTCTTCTTTGATCCAAGGTAGACAAGCTCGAATTGCTGCAACATCTACTATTAATGGACATCTTACAACCATAGATGACACCCTTTATGCAAAAAATTTGCAGATGACTGTAGTTCTTGGAAGGAATGCGTGTACAGCACAGAAATTAGCTCATTATCACTCTGGGGATGGTATTAATATCTTCTGCCTTATAGTACTTTTGTTGGCCATCTGA

Protein Analysis

201

Amino Acids

22.43

Weight (kDa)

6.3

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS-augmin3 PF14932 66 - 192 1.3e-25 HAUS augmin-like complex subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 412
AciI CCGC 1 cut(s) 263
AclWI GGATC 1 cut(s) 397
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 1 cut(s) 487
AcuI CTGAAG 2 cut(s) 297, 345
AcvI CACGTG 1 cut(s) 43
AfaI GTAC 4 cut(s) 57, 64, 526, 588
AfiI CCNNNNNNNGG 2 cut(s) 195, 262
AgsI TTSAA 1 cut(s) 146
AjnI CCWGG 2 cut(s) 193, 255
AluBI AGCT 8 cut(s) 88, 184, 275, 329, 349, 392, 419, 542
AluI AGCT 8 cut(s) 88, 184, 275, 329, 349, 392, 419, 542
Alw26I GTCTC 2 cut(s) 243, 277
AlwI GGATC 1 cut(s) 397
AoxI GGCC 1 cut(s) 597
ApeKI GCWGC 3 cut(s) 272, 346, 428
ApoI RAATTY 1 cut(s) 487
AseI ATTAAT 2 cut(s) 444, 567
AspLEI GCGC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 183
BalI TGGCCA 1 cut(s) 599
BbrPI CACGTG 1 cut(s) 43
BbvI GCAGC 3 cut(s) 284, 358, 415
BccI CCATC 1 cut(s) 554
BciT130I CCWGG 2 cut(s) 195, 257
BcoDI GTCTC 2 cut(s) 243, 277
BfmI CTRYAG 1 cut(s) 501
BisI GCNGC 3 cut(s) 273, 347, 429
BlsI GCNGC 3 cut(s) 274, 348, 430
BmcAI AGTACT 1 cut(s) 588
Bme1390I CCNGG 2 cut(s) 195, 257
BmrFI CCNGG 2 cut(s) 195, 257
BmsI GCATC 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 43
BsaJI CCNNGG 2 cut(s) 194, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 262
Bse1I ACTGG 1 cut(s) 388
BseBI CCWGG 2 cut(s) 195, 257
BseDI CCNNGG 2 cut(s) 194, 406
BseGI GGATG 2 cut(s) 307, 565
BseLI CCNNNNNNNGG 2 cut(s) 195, 262
BseNI ACTGG 1 cut(s) 388
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 3 cut(s) 284, 358, 415
BsgI GTGCAG 1 cut(s) 344
BshFI GGCC 1 cut(s) 599
BsiSI CCGG 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 195, 262
BsmAI GTCTC 2 cut(s) 243, 277
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 1 cut(s) 599
Bsp1407I TGTACA 1 cut(s) 524
Bsp143I GATC 1 cut(s) 402
BspACI CCGC 1 cut(s) 263
BspANI GGCC 1 cut(s) 599
BspPI GGATC 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 524
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 2 cut(s) 194, 406
BssMI GATC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 2 cut(s) 195, 257
Bst4CI ACNGT 2 cut(s) 336, 502
Bst6I CTCTTC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 307, 565
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 1 cut(s) 405
BstMAI GTCTC 2 cut(s) 243, 277
BstMBI GATC 1 cut(s) 402
BstMWI GCNNNNNNNGC 7 cut(s) 94, 161, 269, 311, 346, 425, 527
BstNI CCWGG 2 cut(s) 195, 257
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 193, 255
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 3 cut(s) 284, 358, 415
BsuRI GGCC 1 cut(s) 599
BtsCI GGATG 2 cut(s) 307, 565
CfoI GCGC 1 cut(s) 176
Csp6I GTAC 4 cut(s) 56, 63, 525, 587
CviAII CATG 1 cut(s) 376
CviQI GTAC 4 cut(s) 56, 63, 525, 587
DdeI CTNAG 1 cut(s) 350
DpnI GATC 1 cut(s) 404
DpnII GATC 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 297, 345
Eco72I CACGTG 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 193, 255
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 1 cut(s) 379
FaiI YATR 8 cut(s) 158, 219, 316, 318, 377, 464, 480, 584
FatI CATG 1 cut(s) 375
FblI GTMKAC 1 cut(s) 412
Fnu4HI GCNGC 3 cut(s) 273, 347, 429
FokI GGATG 2 cut(s) 314, 572
Fsp4HI GCNGC 3 cut(s) 273, 347, 429
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 273, 347, 429
GsuI CTGGAG 1 cut(s) 113
HaeIII GGCC 1 cut(s) 599
HapII CCGG 1 cut(s) 18
HhaI GCGC 1 cut(s) 176
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HindIII AAGCTT 3 cut(s) 86, 327, 390
HinfI GANTC 3 cut(s) 23, 31, 285
HpaII CCGG 1 cut(s) 18
HphI GGTGA 1 cut(s) 183
Hpy166II GTNNAC 2 cut(s) 413, 525
Hpy188I TCNGA 4 cut(s) 36, 118, 325, 605
Hpy188III TCNNGA 2 cut(s) 92, 299
Hpy8I GTNNAC 2 cut(s) 413, 525
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 2 cut(s) 336, 502
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 6 cut(s) 305, 340, 361, 431, 482, 493
HpyF10VI GCNNNNNNNGC 7 cut(s) 94, 161, 269, 311, 346, 425, 527
HpyF3I CTNAG 1 cut(s) 350
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 1 cut(s) 402
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 3 cut(s) 284, 358, 415
LweI GCATC 1 cut(s) 292
MaeII ACGT 1 cut(s) 42
MalI GATC 1 cut(s) 404
MboI GATC 1 cut(s) 402
MboII GAAGA 7 cut(s) 4, 158, 212, 231, 290, 387, 565
MfeI CAATTG 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 599
MluCI AATT 5 cut(s) 169, 368, 423, 487, 536
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 4 cut(s) 66, 96, 199, 253
Mox20I TGGCCA 1 cut(s) 599
MscI TGGCCA 1 cut(s) 599
MseI TTAA 2 cut(s) 444, 567
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 2 cut(s) 195, 257
MunI CAATTG 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 2 cut(s) 195, 257
MwoI GCNNNNNNNGC 7 cut(s) 94, 161, 269, 311, 346, 425, 527
NdeII GATC 1 cut(s) 402
NlaIII CATG 1 cut(s) 379
NspI RCATGY 1 cut(s) 379
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 2 cut(s) 23, 31
PkrI GCNGC 3 cut(s) 274, 348, 430
PleI GAGTC 1 cut(s) 279
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 1 cut(s) 279
Ppu21I YACGTR 1 cut(s) 43
PshBI ATTAAT 2 cut(s) 444, 567
Psp6I CCWGG 2 cut(s) 193, 255
PspCI CACGTG 1 cut(s) 43
PspGI CCWGG 2 cut(s) 193, 255
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 4 cut(s) 57, 64, 526, 588
RsaNI GTAC 4 cut(s) 56, 63, 525, 587
SaqAI TTAA 2 cut(s) 444, 567
SatI GCNGC 3 cut(s) 273, 347, 429
Sau3AI GATC 1 cut(s) 402
ScaI AGTACT 1 cut(s) 588
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 2 cut(s) 195, 257
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 5 cut(s) 169, 368, 423, 487, 536
SsiI CCGC 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 193, 255
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 2 cut(s) 336, 502
TaiI ACGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 29, 421
TasI AATT 5 cut(s) 169, 368, 423, 487, 536
TatI WGTACW 3 cut(s) 62, 524, 586
TfiI GAWTC 2 cut(s) 23, 31
Tru1I TTAA 2 cut(s) 444, 567
Tru9I TTAA 2 cut(s) 444, 567
TseI GCWGC 3 cut(s) 272, 346, 428
VspI ATTAAT 2 cut(s) 444, 567
XapI RAATTY 1 cut(s) 487
XceI RCATGY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 412
ZrmI AGTACT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.