RLG00000002827

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013765
Physical Location & Seq
Forward (+)
40381088 .. 40383375
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002827

Sequence Viewer

Length: 813 bp
ATGGTGAAGTTTTTGAAGCCAAACAAGGTTGTGATCCTCTTCCAGGGTCGCATCGCCAGCCGCAAAGGCATCATTGTGAAGGCCATCAACGAAGGCATGCGTGACAATGCCTTGTCGCCGGAATCAAGAAAGACTTGTTGCAGATCTTCTGTTCCAATGTACTTCGATGAAATTTTGGATGAAGCTTTAGATCCAATATCTTGCTCTGGGTTGCAGATTCGACAGAGGGGCGAGATAATTTTGGTTAGATTGTCTGTTAACGCGTGCAGTGCACGCTCTGTTAACAGAGCCCAGGTGACCACCCACTTGGGTGGGCTGAAGAATCTCCGTACAAATAATGCTGTTCTGTTGTTCGACCGTTTCGGGAAAGGTTGTACTGTTGTAGTAGAGACTGATCCGAAATTGCTAGCTTATACTGCTGTCAATGTGACTCTTGAGTTCACCAAACAATCTCTAGCGACAGTGAAGCGGTTTCAAGAGATTGGTGGTTTGAATCCTGTTTTAGCTTCAACAATGGAGGCATGTGCAAATGAACTTACTGTCGCTATGGACGATATCCAGAGTTCCAAAGAGGGATTGGAGGATCATGTAACAGAAGGCTCTGATCCCAAGCTTGAAATGGATAAAACTCAGGCGTGGCTTAATGCTGGACTAACAATAGAGCAGAAATGCTTTGATGGCTTTGCTGTAGATCATCTTGATGGGAAAGTGGAGACCCTTACTCGCAGAAATATTATGAAAGTCGCGCATTTAACAAGCAATGCCGTGCAACTCCTCAACCACTATGCATTATCTAAAGTTACATATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

271

Amino Acids

29.77

Weight (kDa)

8.43

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 133 - 257 1.8e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 263, 746
AciI CCGC 2 cut(s) 61, 469
AclWI GGATC 5 cut(s) 28, 185, 389, 591, 599
AcsI RAATTY 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 338
AfaI GTAC 3 cut(s) 161, 331, 376
AfiI CCNNNNNNNGG 1 cut(s) 43
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 5 cut(s) 16, 476, 493, 510, 617
AjnI CCWGG 2 cut(s) 42, 291
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 4 cut(s) 185, 410, 506, 613
AluI AGCT 4 cut(s) 185, 410, 506, 613
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 2 cut(s) 383, 707
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 5 cut(s) 28, 185, 389, 591, 599
AoxI GGCC 1 cut(s) 81
ApaLI GTGCAC 1 cut(s) 270
ApoI RAATTY 1 cut(s) 171
AspLEI GCGC 1 cut(s) 748
AsuHPI GGTGA 3 cut(s) 16, 307, 433
AsuNHI GCTAGC 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 274
BccI CCATC 3 cut(s) 92, 671, 695
BceAI ACGGC 1 cut(s) 749
BciT130I CCWGG 2 cut(s) 44, 293
BcoDI GTCTC 2 cut(s) 383, 707
BfaI CTAG 2 cut(s) 407, 455
BfmI CTRYAG 1 cut(s) 687
BglI GCCNNNNNGGC 1 cut(s) 66
BglII AGATCT 1 cut(s) 143
BisI GCNGC 1 cut(s) 61
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 44, 293
BmrFI CCNGG 2 cut(s) 44, 293
BmsI GCATC 2 cut(s) 60, 78
BmtI GCTAGC 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 455
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 2 cut(s) 43, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 766
BseBI CCWGG 2 cut(s) 44, 293
BseDI CCNNGG 2 cut(s) 43, 291
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 1 cut(s) 644
BseRI GAGGAG 1 cut(s) 764
BseSI GKGCMC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 286
Bsh1236I CGCG 2 cut(s) 263, 746
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 274
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 2 cut(s) 383, 707
BsnI GGCC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 707
Bsp1286I GDGCHC 2 cut(s) 274, 292
Bsp143I GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
BspACI CCGC 2 cut(s) 61, 469
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 643
BspFNI CGCG 2 cut(s) 263, 746
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 5 cut(s) 28, 185, 389, 591, 599
BspTNI GGTCTC 1 cut(s) 707
BsrDI GCAATG 1 cut(s) 766
BssECI CCNNGG 2 cut(s) 43, 291
BssMI GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
Bst2UI CCWGG 2 cut(s) 44, 293
Bst4CI ACNGT 4 cut(s) 359, 379, 463, 541
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 5 cut(s) 58, 98, 265, 274, 408
BstDEI CTNAG 2 cut(s) 630, 810
BstEII GGTNACC 1 cut(s) 295
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 184
BstFNI CGCG 2 cut(s) 263, 746
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 7 cut(s) 36, 146, 193, 397, 586, 607, 694
BstMAI GTCTC 2 cut(s) 383, 707
BstMBI GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 6 cut(s) 57, 66, 269, 273, 416, 678
BstNI CCWGG 2 cut(s) 44, 293
BstNSI RCATGY 2 cut(s) 100, 525
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 42, 291
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 2 cut(s) 263, 746
BstX2I RGATCY 2 cut(s) 143, 190
BstXI CCANNNNNNTGG 2 cut(s) 307, 311
BstYI RGATCY 2 cut(s) 143, 190
BsuRI GGCC 1 cut(s) 83
BtgZI GCGATG 1 cut(s) 37
BtsCI GGATG 1 cut(s) 184
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 274, 468
Cac8I GCNNGC 5 cut(s) 58, 98, 265, 274, 408
CfoI GCGC 1 cut(s) 748
Csp6I GTAC 3 cut(s) 160, 330, 375
CviAII CATG 3 cut(s) 97, 522, 587
CviQI GTAC 3 cut(s) 160, 330, 375
DdeI CTNAG 2 cut(s) 630, 810
DpnI GATC 7 cut(s) 35, 145, 192, 396, 585, 606, 693
DpnII GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 707
Eco32I GATATC 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 338
Eco91I GGTNACC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 2 cut(s) 42, 291
EcoRV GATATC 1 cut(s) 556
EcoT22I ATGCAT 1 cut(s) 790
EcoT38I GRGCYC 1 cut(s) 292
FaeI CATG 3 cut(s) 100, 525, 590
FaiI YATR 8 cut(s) 98, 414, 523, 548, 588, 737, 786, 805
FalI AAGNNNNNCTT 2 cut(s) 118, 150
FatI CATG 3 cut(s) 96, 521, 586
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 1 cut(s) 61
FspBI CTAG 2 cut(s) 407, 455
GlaI GCGC 1 cut(s) 747
GluI GCNGC 1 cut(s) 61
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 119
HhaI GCGC 1 cut(s) 748
Hin1II CATG 3 cut(s) 100, 525, 590
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HincII GTYRAC 2 cut(s) 259, 283
HindII GTYRAC 2 cut(s) 259, 283
HindIII AAGCTT 2 cut(s) 183, 611
HinfI GANTC 5 cut(s) 122, 217, 322, 430, 493
HpaI GTTAAC 2 cut(s) 259, 283
HpaII CCGG 1 cut(s) 119
HphI GGTGA 3 cut(s) 16, 307, 433
Hpy166II GTNNAC 4 cut(s) 259, 272, 283, 441
Hpy188I TCNGA 2 cut(s) 399, 604
Hpy188III TCNNGA 6 cut(s) 126, 364, 434, 476, 559, 698
Hpy8I GTNNAC 4 cut(s) 259, 272, 283, 441
HpyAV CCTTC 3 cut(s) 73, 86, 590
HpyCH4III ACNGT 4 cut(s) 359, 379, 463, 541
HpyCH4V TGCA 7 cut(s) 141, 214, 267, 272, 527, 769, 788
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 66, 269, 273, 416, 678
HpyF3I CTNAG 2 cut(s) 630, 810
Hsp92II CATG 3 cut(s) 100, 525, 590
HspAI GCGC 1 cut(s) 746
KspAI GTTAAC 2 cut(s) 259, 283
Kzo9I GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
LweI GCATC 2 cut(s) 60, 78
MaeI CTAG 2 cut(s) 407, 455
MaeIII GTNAC 5 cut(s) 101, 295, 427, 589, 799
MalI GATC 7 cut(s) 35, 145, 192, 396, 585, 606, 693
MboI GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
MboII GAAGA 3 cut(s) 31, 138, 331
MflI RGATCY 2 cut(s) 143, 190
MhlI GDGCHC 2 cut(s) 274, 292
MluCI AATT 3 cut(s) 171, 237, 401
MluI ACGCGT 1 cut(s) 261
MlyI GAGTC 1 cut(s) 424
MnlI CCTC 6 cut(s) 47, 219, 511, 565, 574, 785
Mph1103I ATGCAT 1 cut(s) 790
MseI TTAA 4 cut(s) 258, 282, 642, 752
MslI CAYNNNNRTG 3 cut(s) 74, 309, 699
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 2 cut(s) 44, 293
MvaI CCWGG 2 cut(s) 44, 293
MvnI CGCG 2 cut(s) 263, 746
MwoI GCNNNNNNNGC 6 cut(s) 57, 66, 269, 273, 416, 678
NdeII GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
NheI GCTAGC 1 cut(s) 406
NlaIII CATG 3 cut(s) 100, 525, 590
NmuCI GTSAC 3 cut(s) 101, 295, 427
NsiI ATGCAT 1 cut(s) 790
NspI RCATGY 2 cut(s) 100, 525
OliI CACNNNNGTG 1 cut(s) 309
PaeI GCATGC 1 cut(s) 100
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 4 cut(s) 122, 217, 322, 493
PkrI GCNGC 1 cut(s) 62
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 42, 291
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 2 cut(s) 42, 291
PsuI RGATCY 2 cut(s) 143, 190
RsaI GTAC 3 cut(s) 161, 331, 376
RsaNI GTAC 3 cut(s) 160, 330, 375
RseI CAYNNNNRTG 3 cut(s) 74, 309, 699
SaqAI TTAA 4 cut(s) 258, 282, 642, 752
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 7 cut(s) 33, 143, 190, 394, 583, 604, 691
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 44, 293
SduI GDGCHC 2 cut(s) 274, 292
SetI ASST 7 cut(s) 30, 187, 297, 373, 412, 508, 615
SfaNI GCATC 2 cut(s) 60, 78
SfcI CTRYAG 1 cut(s) 687
SmiMI CAYNNNNRTG 3 cut(s) 74, 309, 699
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SphI GCATGC 1 cut(s) 100
Sse9I AATT 3 cut(s) 171, 237, 401
SsiI CCGC 2 cut(s) 61, 469
SspI AATATT 1 cut(s) 733
SspMI CTAG 2 cut(s) 407, 455
StyD4I CCNGG 2 cut(s) 42, 291
TaaI ACNGT 4 cut(s) 359, 379, 463, 541
TaqI TCGA 3 cut(s) 165, 220, 354
TasI AATT 3 cut(s) 171, 237, 401
TatI WGTACW 2 cut(s) 159, 374
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 4 cut(s) 122, 217, 322, 493
Tru1I TTAA 4 cut(s) 258, 282, 642, 752
Tru9I TTAA 4 cut(s) 258, 282, 642, 752
TscAI CASTG 2 cut(s) 274, 468
TseFI GTSAC 3 cut(s) 101, 295, 427
Tsp45I GTSAC 3 cut(s) 101, 295, 427
TspDTI ATGAA 4 cut(s) 183, 195, 546, 752
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 2 cut(s) 274, 468
VneI GTGCAC 1 cut(s) 270
XagI CCTNNNNNAGG 1 cut(s) 41
XapI RAATTY 1 cut(s) 171
XceI RCATGY 2 cut(s) 100, 525
XcmI CCANNNNNNNNNTGG 2 cut(s) 574, 616
XspI CTAG 2 cut(s) 407, 455
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.