Rroxscaffold_5G00332980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013765
Physical Location & Seq
Reverse (-)
384800 .. 387572
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00332980.1

Sequence Viewer

Length: 414 bp
ATGGCAGCAAGGGCGCATTCAAATGGACCAGCCGTGGAGAAGAAGCAAGGTGGTCGGAGTTGTGGGGAAGCCGTAGGTCCGGCTTCCCGAGGTGTTTGCTTTGTCGTCACATCTCTTTATCCGGGTCGTCCGTTGGTAAGGGAGTCGGGGAAGTTTCACCGATTCCGCGGTGCCGGGACTACCGGAGGCTTGGTGGCGGGTTGGTGCGGCGGCGGCAAGATCACGAGTCGCGCTAGATCTTCTGTTCCAATGTACTTCGATGAAATTTTGGATGAAGCTTTAGATCCAATATCTTGCTCTGGGTTGCAAATTCGACAGACGCGCGAGATAATTTTGGTTAGACTGTCACGCTCCGTTAACGAGCCCAGTGACCACCCACTTGGGTGGGCTGAAGAGTCTCCGTATGAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.69

Weight (kDa)

9.07

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AccII CGCG 4 cut(s) 168, 231, 322, 324
AciI CCGC 6 cut(s) 166, 168, 197, 207, 210, 213
AclWI GGATC 1 cut(s) 278
AcsI RAATTY 2 cut(s) 264, 309
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 1 cut(s) 254
AgsI TTSAA 1 cut(s) 21
AleI CACNNNNGTG 1 cut(s) 382
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 264, 309
AspLEI GCGC 3 cut(s) 16, 233, 324
AspS9I GGNCC 2 cut(s) 26, 77
AsuC2I CCSGG 2 cut(s) 123, 175
AsuHPI GGTGA 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 87
AvaII GGWCC 2 cut(s) 26, 77
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 223
BbvI GCAGC 1 cut(s) 17
BceAI ACGGC 2 cut(s) 17, 56
BcgI CGANNNNNNTGC 4 cut(s) 35, 69, 78, 112
BcnI CCSGG 2 cut(s) 123, 175
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 234
BglII AGATCT 1 cut(s) 236
BisI GCNGC 4 cut(s) 6, 208, 211, 214
BlsI GCNGC 4 cut(s) 7, 209, 212, 215
Bme1390I CCNGG 2 cut(s) 123, 175
Bme18I GGWCC 2 cut(s) 26, 77
BmeT110I CYCGRG 1 cut(s) 87
BmgT120I GGNCC 2 cut(s) 26, 77
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 2 cut(s) 123, 175
BmrI ACTGGG 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 360
BpuMI CCSGG 2 cut(s) 123, 175
BsaJI CCNNGG 3 cut(s) 33, 88, 166
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bse1I ACTGG 1 cut(s) 366
BseDI CCNNGG 3 cut(s) 33, 88, 166
BseGI GGATG 1 cut(s) 277
BseNI ACTGG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 4 cut(s) 168, 231, 322, 324
BshNI GGYRCC 1 cut(s) 170
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 4 cut(s) 80, 122, 174, 183
BslFI GGGAC 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 16
BsoBI CYCGRG 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 3 cut(s) 219, 236, 283
BspACI CCGC 6 cut(s) 166, 168, 197, 207, 210, 213
BspFNI CGCG 4 cut(s) 168, 231, 322, 324
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 3 cut(s) 33, 88, 166
BssMI GATC 3 cut(s) 219, 236, 283
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 387
BstDSI CCRYGG 2 cut(s) 33, 166
BstF5I GGATG 1 cut(s) 277
BstFNI CGCG 4 cut(s) 168, 231, 322, 324
BstHHI GCGC 3 cut(s) 16, 233, 324
BstKTI GATC 3 cut(s) 222, 239, 286
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 3 cut(s) 219, 236, 283
BstMWI GCNNNNNNNGC 2 cut(s) 11, 213
BstSCI CCNGG 2 cut(s) 121, 173
BstUI CGCG 4 cut(s) 168, 231, 322, 324
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 236, 283
BstXI CCANNNNNNTGG 2 cut(s) 380, 384
BstYI RGATCY 2 cut(s) 236, 283
BtgI CCRYGG 2 cut(s) 33, 166
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 373
CfoI GCGC 3 cut(s) 16, 233, 324
Cfr13I GGNCC 2 cut(s) 26, 77
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 253
CviJI RGCY 7 cut(s) 32, 71, 83, 189, 278, 364, 389
CviKI_1 RGCY 7 cut(s) 32, 71, 83, 189, 278, 364, 389
CviQI GTAC 1 cut(s) 253
DpnI GATC 3 cut(s) 221, 238, 285
DpnII GATC 3 cut(s) 219, 236, 283
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 366
Eco47I GGWCC 2 cut(s) 26, 77
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 366
FaiI YATR 1 cut(s) 405
FaqI GGGAC 1 cut(s) 190
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 4 cut(s) 6, 208, 211, 214
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 6, 208, 211, 214
FspBI CTAG 1 cut(s) 234
GlaI GCGC 3 cut(s) 15, 232, 323
GluI GCNGC 4 cut(s) 6, 208, 211, 214
HapII CCGG 4 cut(s) 80, 122, 174, 183
HgaI GACGC 1 cut(s) 328
HhaI GCGC 3 cut(s) 16, 233, 324
Hin6I GCGC 3 cut(s) 14, 231, 322
HinP1I GCGC 3 cut(s) 14, 231, 322
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 4 cut(s) 143, 162, 226, 395
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 4 cut(s) 80, 122, 174, 183
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 87, 223
Hpy8I GTNNAC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4V TGCA 1 cut(s) 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 213
HspAI GCGC 3 cut(s) 14, 231, 322
KspAI GTTAAC 1 cut(s) 358
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 3 cut(s) 219, 236, 283
LmnI GCTCC 1 cut(s) 356
LpnPI CCDG 7 cut(s) 42, 93, 135, 187, 196, 285, 379
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 1 cut(s) 234
MaeIII GTNAC 3 cut(s) 106, 345, 368
MalI GATC 3 cut(s) 221, 238, 285
MboI GATC 3 cut(s) 219, 236, 283
MboII GAAGA 3 cut(s) 52, 231, 404
MflI RGATCY 2 cut(s) 236, 283
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 3 cut(s) 264, 309, 330
MlyI GAGTC 3 cut(s) 152, 235, 404
MmeI TCCRAC 1 cut(s) 35
MnlI CCTC 2 cut(s) 83, 179
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 2 cut(s) 21, 382
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 4 cut(s) 80, 122, 174, 183
MspR9I CCNGG 2 cut(s) 123, 175
Mva1269I GAATGC 1 cut(s) 16
MvnI CGCG 4 cut(s) 168, 231, 322, 324
MwoI GCNNNNNNNGC 2 cut(s) 11, 213
NciI CCSGG 2 cut(s) 123, 175
NdeII GATC 3 cut(s) 219, 236, 283
NlaIV GGNNCC 1 cut(s) 172
NmuCI GTSAC 3 cut(s) 106, 345, 368
OliI CACNNNNGTG 1 cut(s) 382
PctI GAATGC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 4 cut(s) 7, 209, 212, 215
PleI GAGTC 3 cut(s) 151, 234, 403
PpsI GAGTC 3 cut(s) 151, 234, 403
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 2 cut(s) 26, 77
PsuI RGATCY 2 cut(s) 236, 283
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 2 cut(s) 21, 382
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 4 cut(s) 6, 208, 211, 214
Sau3AI GATC 3 cut(s) 219, 236, 283
Sau96I GGNCC 2 cut(s) 26, 77
SchI GAGTC 3 cut(s) 152, 235, 404
ScrFI CCNGG 2 cut(s) 123, 175
SduI GDGCHC 1 cut(s) 366
SetI ASST 4 cut(s) 52, 79, 94, 280
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 2 cut(s) 26, 77
SmiMI CAYNNNNRTG 2 cut(s) 21, 382
Sse9I AATT 3 cut(s) 264, 309, 330
SsiI CCGC 6 cut(s) 166, 168, 197, 207, 210, 213
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 121, 173
TaaI ACNGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 258, 313
TasI AATT 3 cut(s) 264, 309, 330
TatI WGTACW 1 cut(s) 252
TauI GCSGC 3 cut(s) 210, 213, 216
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 3 cut(s) 106, 345, 368
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 106, 345, 368
TspDTI ATGAA 2 cut(s) 276, 288
TspGWI ACGGA 3 cut(s) 120, 343, 390
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 2 cut(s) 26, 77
XapI RAATTY 2 cut(s) 264, 309
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.