Rh7BG267300

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013765
Physical Location & Seq
Reverse (-)
24494799 .. 24496087
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG267300.1

Sequence Viewer

Length: 792 bp
ATGGAAGGCTATCTTGTACGTACACTTTTAGCTTTCGCCATTCTCTCATTGTTTAACCACATAAACCCCTGCACAGCAACCTTGCTTCCTAATGAACCAAACACCGACTTCATAAGAACTTCTTGCAAAGTCACATTACATCCCATTTTGTGCTATAATTCTCTCAAGGATTACGCATGCAAAATCCAGACTGATCCGAAATTGCTAGCTTATACTGCTATCAATGTGACTCTTGAGTTCACCAAACAATCTCTAGCGACAGTCAAGCGGTTTCAAGAGATTGGTGGTTTGAATCCTGCTTTAGCTTCAACAATGGAGGCATGTGCAAATGAACTTACTGTCGCTATGGACGACATCCAGAATTCCAAAGAGGGATTGGAGGATCATGTAACAGAAGGCTCTGATCCCAAGCTTGAAATGGGTGATACTCAGGCGTGGCTTAATGCCGGACTAACAATAGAGCAGAAATGCTTTGATGGCTTTGCTGTAGATCATCTTGATGGGAAAGTGGAGACCCTTACTCGCAGAAATATTATGAAAGTCGCGCATTTAACAAGCAATGCCGTGCAACTCCTCAACCACTATGCATTATCTAAAGTTCCACGGTTTGTTGCCCGTGAGGAATTAACCACTCATATAGGTTGGGGGTGGCCATGTTGGAGGATCAACATAGATACGAGGAAAGTGTGTTTCAATTCAATCGTCATGCTCAGGCTGGAGCTGGATCAAGCTGATAAACCAGGAGCCTTAGGACTCGTCGAAATTGGAGAAGGTACCACAGCCTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

263

Amino Acids

29.21

Weight (kDa)

5.4

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 36 - 190 2.5e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 773
AccB1I GGYRCC 1 cut(s) 773
AccII CGCG 1 cut(s) 545
AciI CCGC 1 cut(s) 268
AclWI GGATC 5 cut(s) 188, 390, 398, 671, 732
AcoI YGGCCR 1 cut(s) 650
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 3 cut(s) 18, 22, 775
AgsI TTSAA 6 cut(s) 275, 292, 309, 416, 694, 699
AjnI CCWGG 1 cut(s) 739
AluBI AGCT 6 cut(s) 32, 209, 305, 412, 721, 731
AluI AGCT 6 cut(s) 32, 209, 305, 412, 721, 731
Alw26I GTCTC 1 cut(s) 506
AlwI GGATC 5 cut(s) 188, 390, 398, 671, 732
AoxI GGCC 1 cut(s) 650
ApoI RAATTY 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 773
AspLEI GCGC 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 232, 434
AsuNHI GCTAGC 1 cut(s) 205
AxyI CCTNAGG 1 cut(s) 748
BalI TGGCCA 1 cut(s) 652
BanI GGYRCC 1 cut(s) 773
BccI CCATC 2 cut(s) 470, 494
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 506
BfaI CTAG 2 cut(s) 206, 254
BfmI CTRYAG 1 cut(s) 486
Bme1390I CCNGG 1 cut(s) 741
BmiI GGNNCC 2 cut(s) 745, 775
BmrFI CCNGG 1 cut(s) 741
BmtI GCTAGC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 737
Bpu10I CCTNAGC 1 cut(s) 710
BpuEI CTTGAG 2 cut(s) 149, 254
BsaAI YACGTR 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 2 cut(s) 602, 783
Bse21I CCTNAGG 1 cut(s) 748
Bse3DI GCAATG 1 cut(s) 565
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 602, 783
BseGI GGATG 2 cut(s) 139, 354
BseMI GCAATG 1 cut(s) 565
BseMII CTCAG 2 cut(s) 443, 724
BseRI GAGGAG 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 1 cut(s) 652
BshNI GGYRCC 1 cut(s) 773
BsiSI CCGG 1 cut(s) 447
BsmAI GTCTC 1 cut(s) 506
BsnI GGCC 1 cut(s) 652
Bso31I GGTCTC 1 cut(s) 506
Bsp143I GATC 6 cut(s) 193, 382, 403, 490, 663, 724
BspACI CCGC 1 cut(s) 268
BspANI GGCC 1 cut(s) 652
BspCNI CTCAG 2 cut(s) 442, 723
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 2 cut(s) 745, 775
BspOI GCTAGC 1 cut(s) 209
BspPI GGATC 5 cut(s) 188, 390, 398, 671, 732
BspT107I GGYRCC 1 cut(s) 773
BspTNI GGTCTC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 565
BssECI CCNNGG 2 cut(s) 602, 783
BssMI GATC 6 cut(s) 193, 382, 403, 490, 663, 724
BssT1I CCWWGG 1 cut(s) 783
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 3 cut(s) 262, 340, 606
BstBAI YACGTR 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 178, 207
BstDEI CTNAG 3 cut(s) 429, 710, 748
BstDSI CCRYGG 1 cut(s) 602
BstF5I GGATG 2 cut(s) 139, 354
BstFNI CGCG 1 cut(s) 545
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 6 cut(s) 196, 385, 406, 493, 666, 727
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 6 cut(s) 193, 382, 403, 490, 663, 724
BstMWI GCNNNNNNNGC 2 cut(s) 215, 477
BstNI CCWGG 1 cut(s) 741
BstNSI RCATGY 2 cut(s) 180, 324
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 486
BstSNI TACGTA 1 cut(s) 20
BstUI CGCG 1 cut(s) 545
BstXI CCANNNNNNTGG 1 cut(s) 784
Bsu36I CCTNAGG 1 cut(s) 748
BsuRI GGCC 1 cut(s) 652
BtgI CCRYGG 1 cut(s) 602
BtsCI GGATG 2 cut(s) 139, 354
Cac8I GCNNGC 2 cut(s) 178, 207
CfoI GCGC 1 cut(s) 547
Csp6I GTAC 3 cut(s) 17, 21, 774
CviAII CATG 5 cut(s) 177, 321, 386, 654, 706
CviQI GTAC 3 cut(s) 17, 21, 774
DdeI CTNAG 3 cut(s) 429, 710, 748
DpnI GATC 6 cut(s) 195, 384, 405, 492, 665, 726
DpnII GATC 6 cut(s) 193, 382, 403, 490, 663, 724
EaeI YGGCCR 1 cut(s) 650
Eco105I TACGTA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 783
Eco31I GGTCTC 1 cut(s) 506
Eco81I CCTNAGG 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 783
EcoT22I ATGCAT 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 783
FaeI CATG 5 cut(s) 180, 324, 389, 657, 709
FalI AAGNNNNNCTT 3 cut(s) 29, 106, 138
FatI CATG 5 cut(s) 176, 320, 385, 653, 705
FokI GGATG 2 cut(s) 126, 341
FspBI CTAG 2 cut(s) 206, 254
GlaI GCGC 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 737
HaeIII GGCC 1 cut(s) 652
HapII CCGG 1 cut(s) 447
HhaI GCGC 1 cut(s) 547
Hin1II CATG 5 cut(s) 180, 324, 389, 657, 709
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 3 cut(s) 229, 292, 753
HpaII CCGG 1 cut(s) 447
HphI GGTGA 2 cut(s) 232, 434
Hpy166II GTNNAC 2 cut(s) 23, 240
Hpy188I TCNGA 2 cut(s) 198, 403
Hpy188III TCNNGA 5 cut(s) 187, 233, 275, 358, 497
Hpy8I GTNNAC 2 cut(s) 23, 240
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 2 cut(s) 389, 764
HpyCH4III ACNGT 3 cut(s) 262, 340, 606
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 6 cut(s) 72, 126, 180, 326, 568, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 477
HpyF3I CTNAG 3 cut(s) 429, 710, 748
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 5 cut(s) 180, 324, 389, 657, 709
HspAI GCGC 1 cut(s) 545
KpnI GGTACC 1 cut(s) 777
Kzo9I GATC 6 cut(s) 193, 382, 403, 490, 663, 724
LmnI GCTCC 2 cut(s) 718, 743
MaeI CTAG 2 cut(s) 206, 254
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 3 cut(s) 130, 226, 388
MalI GATC 6 cut(s) 195, 384, 405, 492, 665, 726
MboI GATC 6 cut(s) 193, 382, 403, 490, 663, 724
MlsI TGGCCA 1 cut(s) 652
MluCI AATT 6 cut(s) 157, 200, 361, 623, 694, 762
MluNI TGGCCA 1 cut(s) 652
MlyI GAGTC 2 cut(s) 223, 747
MmeI TCCRAC 1 cut(s) 638
MnlI CCTC 7 cut(s) 310, 364, 373, 584, 613, 654, 672
Mox20I TGGCCA 1 cut(s) 652
Mph1103I ATGCAT 1 cut(s) 589
MscI TGGCCA 1 cut(s) 652
MseI TTAA 4 cut(s) 54, 441, 551, 626
MslI CAYNNNNRTG 1 cut(s) 498
Msp20I TGGCCA 1 cut(s) 652
MspI CCGG 1 cut(s) 447
MspR9I CCNGG 1 cut(s) 741
MvaI CCWGG 1 cut(s) 741
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 215, 477
NdeII GATC 6 cut(s) 193, 382, 403, 490, 663, 724
NheI GCTAGC 1 cut(s) 205
NlaIII CATG 5 cut(s) 180, 324, 389, 657, 709
NlaIV GGNNCC 2 cut(s) 745, 775
NmuCI GTSAC 2 cut(s) 130, 226
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 2 cut(s) 180, 324
PaeI GCATGC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 1 cut(s) 292
PleI GAGTC 2 cut(s) 223, 747
PpsI GAGTC 2 cut(s) 223, 747
Ppu21I YACGTR 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 2 cut(s) 745, 775
RsaI GTAC 3 cut(s) 18, 22, 775
RsaNI GTAC 3 cut(s) 17, 21, 774
RseI CAYNNNNRTG 1 cut(s) 498
SaqAI TTAA 4 cut(s) 54, 441, 551, 626
Sau3AI GATC 6 cut(s) 193, 382, 403, 490, 663, 724
SchI GAGTC 2 cut(s) 223, 747
ScrFI CCNGG 1 cut(s) 741
SfcI CTRYAG 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 2 cut(s) 164, 233
SmoI CTYRAG 2 cut(s) 164, 233
SnaBI TACGTA 1 cut(s) 20
SphI GCATGC 1 cut(s) 180
Sse9I AATT 6 cut(s) 157, 200, 361, 623, 694, 762
SsiI CCGC 1 cut(s) 268
SspI AATATT 1 cut(s) 532
SspMI CTAG 2 cut(s) 206, 254
StyD4I CCNGG 1 cut(s) 739
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 3 cut(s) 262, 340, 606
TaiI ACGT 1 cut(s) 22
TaqI TCGA 1 cut(s) 759
TasI AATT 6 cut(s) 157, 200, 361, 623, 694, 762
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 4 cut(s) 54, 441, 551, 626
Tru9I TTAA 4 cut(s) 54, 441, 551, 626
TseFI GTSAC 2 cut(s) 130, 226
Tsp45I GTSAC 2 cut(s) 130, 226
TspDTI ATGAA 4 cut(s) 100, 108, 345, 551
XapI RAATTY 1 cut(s) 361
XceI RCATGY 2 cut(s) 180, 324
XcmI CCANNNNNNNNNTGG 2 cut(s) 373, 415
XspI CTAG 2 cut(s) 206, 254
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.