RLG00000006339

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012695
Physical Location & Seq
Reverse (-)
6342883 .. 6355332
12450 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006339

Sequence Viewer

Length: 1203 bp
ATGTCTTTGGTGGTGTTGGGCTGGGACGTCCTCGACTCAGGCTACGGGGATGCAGGTATGCCACGCTTTGTGGAGGCAACAGGTGGACCGAGGCGAGTCTCTCAAGCGTCGACGATGACTAGGCCGGTGGTTGAGACTGGTGGCCTACTAGAGGTGGACCGTCTCCTGCTGGTGCAGGTAGCTTTAGCATATCAACTTGCTGAAAGTGGCAGCGAGGGTTCATCAGATGGAAGCGAGGAGAATGGTAACCACCAGGAGTATGGTGCAAACAAGAAGGGAAGCTTTGACAAGATGCTTGCAGATGGAGCAAATGCACAAAATAACACAGCTTTAGTGCCTGGGAAGCCTGTAGTTTCTGTGCCTGCAACTAGTCTGAATATGGGAATGGACTGTGGAATGCATCCCCCGCTGGGAGCTTTGGACGGTGGTTTGGATATTCCACACAGTGATAAGAGGTTTGCTGGTTTCTCCAAGGACAACAAGCAGCTTGATGCTGAGGTACATCGCAAGTTTATCTATGGTGGCCATGTTGCTGCATACATGAGGATACCTCTCAATGCAGTTACATCTATAACTGATGGCTTGAAGAAATTGTACATTGAAAAACCAAAACCATTGGAAGCTGCATATCGTTTCAATGATTTTGGTAATCCATTACTGCCTGCTTCAACAAAATTGCAATCGTATGCAGGGTCGCAGCCATTACCTTCGGCCTCGTATCCGTCATCACCGTTGGATTCCGTAGTCTGGTTCATCTTCCATTTGGATGGAACTGGAACGAAGTCGTGGTCATTGAAACTCTGCCTCTGTGGCCAAACTCTGATCACAAGATTTGAACCAGAGCTCTATTCTTTGAGAAGCCCAACAATGTCGGTGAATCGGAGACCGAGGAACTCCGGCGAACATAGTCGAGGTCCGAAAGAATCGTCAGAGAGAATAAGCTTTCGGGAAGAGAGTACGTGGCGCCAGATTCTTGAGAGTGGTTCCCTTTCCTTCGTCACGAACCATGACTCAATATTTGAGGTAACGAGTGTTGGAATCAATACTGAATGGGGATTGTTGATGGCAAGTATATCTCTGAGGATACCTATAAAGAAACTCTTCTACAGATTTGTGTTCCTTCCAATTTCTAACCTGAAACTGTTGAGGATGCTAGCTAATGAAGGTCCTGCAGTGTTGTGTTCCTTGCAATCTCTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

401

Amino Acids

43.6

Weight (kDa)

7.09

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EHD_N PF16880 194 - 220 2.4e-08 N-terminal EH-domain containing protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
Acc36I ACCTGC 2 cut(s) 44, 166
AccB1I GGYRCC 1 cut(s) 963
AccI GTMKAC 1 cut(s) 110
AciI CCGC 1 cut(s) 407
AcoI YGGCCR 2 cut(s) 523, 811
AcyI GRCGYC 2 cut(s) 27, 964
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 3 cut(s) 501, 596, 958
AfiI CCNNNNNNNGG 3 cut(s) 151, 410, 747
AgsI TTSAA 6 cut(s) 586, 602, 637, 669, 796, 836
AhlI ACTAGT 1 cut(s) 368
AjnI CCWGG 2 cut(s) 252, 337
AluBI AGCT 9 cut(s) 182, 282, 329, 416, 487, 623, 844, 942, 1157
AluI AGCT 9 cut(s) 182, 282, 329, 416, 487, 623, 844, 942, 1157
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 4 cut(s) 103, 128, 167, 877
AoxI GGCC 5 cut(s) 122, 142, 523, 711, 811
ApeKI GCWGC 5 cut(s) 210, 484, 533, 623, 697
ArsI GACNNNNNNTTYG 6 cut(s) 773, 805, 911, 943, 1001, 1033
Asp700I GAANNNNTTC 1 cut(s) 1100
AspLEI GCGC 1 cut(s) 966
AspS9I GGNCC 4 cut(s) 86, 157, 914, 1166
AsuHPI GGTGA 2 cut(s) 720, 886
AsuNHI GCTAGC 1 cut(s) 1153
AvaII GGWCC 4 cut(s) 86, 157, 914, 1166
BalI TGGCCA 2 cut(s) 525, 813
BanI GGYRCC 1 cut(s) 963
BanII GRGCYC 1 cut(s) 846
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
Bbv12I GWGCWC 1 cut(s) 846
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 222, 496, 520, 610, 709
BccI CCATC 5 cut(s) 221, 296, 572, 761, 1057
BciT130I CCWGG 2 cut(s) 254, 339
BciVI GTATCC 3 cut(s) 540, 729, 1077
BclI TGATCA 1 cut(s) 822
BcoDI GTCTC 4 cut(s) 103, 128, 167, 877
BcuI ACTAGT 1 cut(s) 368
BfaI CTAG 4 cut(s) 120, 149, 369, 1154
BfmI CTRYAG 3 cut(s) 348, 1105, 1170
BfoI RGCGCY 1 cut(s) 967
BfuAI ACCTGC 2 cut(s) 44, 166
BfuI GTATCC 3 cut(s) 540, 729, 1077
BglI GCCNNNNNGGC 1 cut(s) 810
BisI GCNGC 5 cut(s) 211, 485, 534, 624, 698
BlsI GCNGC 5 cut(s) 212, 486, 535, 625, 699
Bme1390I CCNGG 2 cut(s) 254, 339
Bme18I GGWCC 4 cut(s) 86, 157, 914, 1166
BmgT120I GGNCC 4 cut(s) 86, 157, 914, 1166
BmiI GGNNCC 2 cut(s) 965, 985
BmrFI CCNGG 2 cut(s) 254, 339
BmsI GCATC 5 cut(s) 40, 282, 409, 481, 1140
BmtI GCTAGC 1 cut(s) 1157
BplI GAGNNNNNCTC 2 cut(s) 535, 567
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 87, 995
BsaAI YACGTR 1 cut(s) 960
BsaHI GRCGYC 2 cut(s) 27, 964
BsaI GGTCTC 1 cut(s) 877
BsaJI CCNNGG 4 cut(s) 89, 338, 471, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 410, 747
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 2 cut(s) 142, 778
BseBI CCWGG 2 cut(s) 254, 339
BseDI CCNNGG 4 cut(s) 89, 338, 471, 887
BseGI GGATG 4 cut(s) 55, 400, 772, 1155
BseLI CCNNNNNNNGG 3 cut(s) 151, 410, 747
BseMII CTCAG 3 cut(s) 51, 486, 1070
BseNI ACTGG 2 cut(s) 142, 778
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 5 cut(s) 222, 496, 520, 610, 709
BseYI CCCAGC 2 cut(s) 21, 409
BsgI GTGCAG 1 cut(s) 194
BshFI GGCC 5 cut(s) 124, 144, 525, 713, 813
BshNI GGYRCC 1 cut(s) 963
BsiHKAI GWGCWC 1 cut(s) 846
BsiSI CCGG 2 cut(s) 125, 897
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 3 cut(s) 151, 410, 747
BsmAI GTCTC 4 cut(s) 103, 128, 167, 877
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 5 cut(s) 124, 144, 525, 713, 813
Bso31I GGTCTC 1 cut(s) 877
Bsp1286I GDGCHC 1 cut(s) 846
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 1 cut(s) 822
BspACI CCGC 1 cut(s) 407
BspANI GGCC 5 cut(s) 124, 144, 525, 713, 813
BspCNI CTCAG 3 cut(s) 50, 487, 1071
BspLI GGNNCC 2 cut(s) 965, 985
BspMAI CTGCAG 1 cut(s) 1174
BspMI ACCTGC 2 cut(s) 44, 166
BspOI GCTAGC 1 cut(s) 1157
BspT107I GGYRCC 1 cut(s) 963
BspTNI GGTCTC 1 cut(s) 877
BsrFI RCCGGY 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 2 cut(s) 142, 778
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 4 cut(s) 89, 338, 471, 887
BssMI GATC 1 cut(s) 822
BssNI GRCGYC 2 cut(s) 27, 964
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 2 cut(s) 254, 339
Bst4CI ACNGT 6 cut(s) 161, 392, 425, 446, 732, 1143
Bst6I CTCTTC 2 cut(s) 945, 1106
BstACI GRCGYC 2 cut(s) 27, 964
BstAUI TGTACA 1 cut(s) 594
BstBAI YACGTR 1 cut(s) 960
BstC8I GCNNGC 4 cut(s) 297, 363, 663, 1155
BstDEI CTNAG 3 cut(s) 37, 495, 1079
BstEII GGTNACC 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 149
BstF5I GGATG 4 cut(s) 55, 400, 772, 1155
BstH2I RGCGCY 1 cut(s) 967
BstHHI GCGC 1 cut(s) 966
BstKTI GATC 1 cut(s) 825
BstMAI GTCTC 4 cut(s) 103, 128, 167, 877
BstMBI GATC 1 cut(s) 822
BstMWI GCNNNNNNNGC 4 cut(s) 305, 343, 406, 810
BstNI CCWGG 2 cut(s) 254, 339
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 2 cut(s) 252, 337
BstSFI CTRYAG 3 cut(s) 348, 1105, 1170
BstV1I GCAGC 5 cut(s) 222, 496, 520, 610, 709
BstXI CCANNNNNNTGG 2 cut(s) 260, 767
BsuI GTATCC 3 cut(s) 540, 729, 1077
BsuRI GGCC 5 cut(s) 124, 144, 525, 713, 813
BtgZI GCGATG 1 cut(s) 488
BtsCI GGATG 4 cut(s) 55, 400, 772, 1155
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 2 cut(s) 451, 1179
BveI ACCTGC 2 cut(s) 44, 166
Cac8I GCNNGC 4 cut(s) 297, 363, 663, 1155
CfoI GCGC 1 cut(s) 966
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 4 cut(s) 86, 157, 914, 1166
CseI GACGC 1 cut(s) 96
Csp6I GTAC 3 cut(s) 500, 595, 957
CviAII CATG 3 cut(s) 527, 541, 1007
CviQI GTAC 3 cut(s) 500, 595, 957
DdeI CTNAG 3 cut(s) 37, 495, 1079
DinI GGCGCC 1 cut(s) 965
DpnI GATC 1 cut(s) 824
DpnII GATC 1 cut(s) 822
DraIII CACNNNGTG 1 cut(s) 446
EaeI YGGCCR 2 cut(s) 523, 811
Eam1104I CTCTTC 2 cut(s) 945, 1106
EarI CTCTTC 2 cut(s) 945, 1106
Ecl136II GAGCTC 1 cut(s) 844
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 846
Eco31I GGTCTC 1 cut(s) 877
Eco47I GGWCC 4 cut(s) 86, 157, 914, 1166
Eco53kI GAGCTC 1 cut(s) 844
Eco91I GGTNACC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 844
EcoNI CCTNNNNNAGG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 1166
EcoO65I GGTNACC 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 252, 337
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 846
EgeI GGCGCC 1 cut(s) 965
EheI GGCGCC 1 cut(s) 965
ErhI CCWWGG 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 167
FaeI CATG 3 cut(s) 530, 544, 1010
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 1085, 1117
FaqI GGGAC 1 cut(s) 38
FatI CATG 3 cut(s) 526, 540, 1006
FauI CCCGC 1 cut(s) 414
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 1 cut(s) 110
Fnu4HI GCNGC 5 cut(s) 211, 485, 534, 624, 698
FokI GGATG 4 cut(s) 62, 387, 779, 1162
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 5 cut(s) 211, 485, 534, 624, 698
FspBI CTAG 4 cut(s) 120, 149, 369, 1154
GlaI GCGC 1 cut(s) 965
GluI GCNGC 5 cut(s) 211, 485, 534, 624, 698
GsaI CCCAGC 2 cut(s) 25, 413
HaeII RGCGCY 1 cut(s) 967
HaeIII GGCC 5 cut(s) 124, 144, 525, 713, 813
HapII CCGG 2 cut(s) 125, 897
HgaI GACGC 1 cut(s) 96
HhaI GCGC 1 cut(s) 966
Hin1I GRCGYC 2 cut(s) 27, 964
Hin1II CATG 3 cut(s) 530, 544, 1010
Hin6I GCGC 1 cut(s) 964
HinP1I GCGC 1 cut(s) 964
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 2 cut(s) 280, 940
HinfI GANTC 8 cut(s) 35, 96, 737, 877, 923, 970, 1010, 1038
HpaII CCGG 2 cut(s) 125, 897
HphI GGTGA 2 cut(s) 720, 886
Hpy166II GTNNAC 3 cut(s) 86, 111, 157
Hpy188I TCNGA 8 cut(s) 226, 375, 822, 882, 918, 931, 1080, 1197
Hpy188III TCNNGA 3 cut(s) 947, 974, 1000
Hpy8I GTNNAC 3 cut(s) 86, 111, 157
Hpy99I CGWCG 2 cut(s) 112, 115
HpyAV CCTTC 5 cut(s) 268, 717, 1003, 1130, 1157
HpyCH4III ACNGT 6 cut(s) 161, 392, 425, 446, 732, 1143
HpyCH4IV ACGT 2 cut(s) 27, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 343, 406, 810
HpyF3I CTNAG 3 cut(s) 37, 495, 1079
HpySE526I ACGT 2 cut(s) 27, 959
Hsp92I GRCGYC 2 cut(s) 27, 964
Hsp92II CATG 3 cut(s) 530, 544, 1010
HspAI GCGC 1 cut(s) 964
KasI GGCGCC 1 cut(s) 963
Ksp22I TGATCA 1 cut(s) 822
Kzo9I GATC 1 cut(s) 822
LmnI GCTCC 2 cut(s) 305, 413
Lsp1109I GCAGC 5 cut(s) 222, 496, 520, 610, 709
LweI GCATC 5 cut(s) 40, 282, 409, 481, 1140
MaeI CTAG 4 cut(s) 120, 149, 369, 1154
MaeII ACGT 2 cut(s) 27, 959
MaeIII GTNAC 4 cut(s) 245, 562, 997, 1024
MalI GATC 1 cut(s) 824
MboI GATC 1 cut(s) 822
MboII GAAGA 4 cut(s) 598, 748, 962, 1093
MhlI GDGCHC 1 cut(s) 846
MlsI TGGCCA 2 cut(s) 525, 813
MluCI AATT 3 cut(s) 590, 674, 1125
MluNI TGGCCA 2 cut(s) 525, 813
Mly113I GGCGCC 1 cut(s) 964
MlyI GAGTC 3 cut(s) 29, 105, 1004
MmeI TCCRAC 2 cut(s) 714, 1015
Mox20I TGGCCA 2 cut(s) 525, 813
Mph1103I ATGCAT 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 1100
MscI TGGCCA 2 cut(s) 525, 813
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 2 cut(s) 525, 813
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 2 cut(s) 125, 897
MspR9I CCNGG 2 cut(s) 254, 339
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 2 cut(s) 254, 339
MwoI GCNNNNNNNGC 4 cut(s) 305, 343, 406, 810
NarI GGCGCC 1 cut(s) 964
NdeII GATC 1 cut(s) 822
NheI GCTAGC 1 cut(s) 1153
NlaIII CATG 3 cut(s) 530, 544, 1010
NlaIV GGNNCC 2 cut(s) 965, 985
NmuCI GTSAC 1 cut(s) 997
NsiI ATGCAT 1 cut(s) 402
PctI GAATGC 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 1100
PfeI GAWTC 5 cut(s) 737, 877, 923, 970, 1038
PkrI GCNGC 5 cut(s) 212, 486, 535, 625, 699
PleI GAGTC 3 cut(s) 29, 104, 1004
PluTI GGCGCC 1 cut(s) 967
PpsI GAGTC 3 cut(s) 29, 104, 1004
Ppu21I YACGTR 1 cut(s) 960
PpuMI RGGWCCY 1 cut(s) 1166
Psp124BI GAGCTC 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 1166
Psp6I CCWGG 2 cut(s) 252, 337
PspEI GGTNACC 1 cut(s) 245
PspFI CCCAGC 2 cut(s) 21, 409
PspGI CCWGG 2 cut(s) 252, 337
PspN4I GGNNCC 2 cut(s) 965, 985
PspPI GGNCC 4 cut(s) 86, 157, 914, 1166
PspPPI RGGWCCY 1 cut(s) 1166
PstI CTGCAG 1 cut(s) 1174
RsaI GTAC 3 cut(s) 501, 596, 958
RsaNI GTAC 3 cut(s) 500, 595, 957
RseI CAYNNNNRTG 1 cut(s) 765
SacI GAGCTC 1 cut(s) 846
SalI GTCGAC 1 cut(s) 109
SatI GCNGC 5 cut(s) 211, 485, 534, 624, 698
Sau3AI GATC 1 cut(s) 822
Sau96I GGNCC 4 cut(s) 86, 157, 914, 1166
SchI GAGTC 3 cut(s) 29, 105, 1004
ScrFI CCNGG 2 cut(s) 254, 339
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 5 cut(s) 40, 282, 409, 481, 1140
SfcI CTRYAG 3 cut(s) 348, 1105, 1170
SfoI GGCGCC 1 cut(s) 965
SgrDI CGTCGACG 1 cut(s) 109
SinI GGWCC 4 cut(s) 86, 157, 914, 1166
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 102, 974
SmoI CTYRAG 2 cut(s) 102, 974
SpeI ACTAGT 1 cut(s) 368
Sse9I AATT 3 cut(s) 590, 674, 1125
SsiI CCGC 1 cut(s) 407
SspDI GGCGCC 1 cut(s) 963
SspI AATATT 1 cut(s) 1017
SspMI CTAG 4 cut(s) 120, 149, 369, 1154
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 2 cut(s) 252, 337
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 6 cut(s) 161, 392, 425, 446, 732, 1143
TaiI ACGT 2 cut(s) 30, 962
TaqI TCGA 3 cut(s) 33, 110, 910
TaqII GACCGA 2 cut(s) 103, 901
TasI AATT 3 cut(s) 590, 674, 1125
TatI WGTACW 1 cut(s) 594
TfiI GAWTC 5 cut(s) 737, 877, 923, 970, 1038
TscAI CASTG 2 cut(s) 451, 1179
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 5 cut(s) 210, 484, 533, 623, 697
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 3 cut(s) 210, 742, 1176
TspGWI ACGGA 2 cut(s) 711, 730
TspRI CASTG 2 cut(s) 451, 1179
VpaK11BI GGWCC 4 cut(s) 86, 157, 914, 1166
XagI CCTNNNNNAGG 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 1100
XspI CTAG 4 cut(s) 120, 149, 369, 1154
ZraI GACGTC 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.