RLG00000007587

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009932
Physical Location & Seq
Forward (+)
18727755 .. 18731632
3878 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007587

Sequence Viewer

Length: 744 bp
ATGGCCATCGTTAGCCTTGTGGCTGGTTGTTTTTGTGTTCTCATCGCATTCACTCTGGGTGTAAGACAGTCACCAATCTTTGGGATGCCATCTGGCTTTCCTTTAGGCTTCAGCAACGTGTTGCCGATGTTGGATTCGAGCCCGGGCAATGTCTGTTGCCATAGAGCGCTTGTGAATTACTACGGCGATGGTGCGGGTCCGGTTTCGAGTAGCAGCAGTGGGGTTTCGAGCTCTGCAAGAAGTCCTCCTATGAGAATTGCTTATAGCTACCAGTTTCTCAAGATGGATCGAATTCTCTCTCTCTTCCTCTTCTTCCTCTCTCCTGCCGATGTGGACGGTGGCTATCAATTGATCCGTCAAGTCGTGGAAGGCGGCGATGAAGCCCTTCACCACCACGATGATCATGACGGCGAACAAATGCTCAGAGCTGAGCACCACTTCTCGCTGTTCAAGAACAAATTCGGCAAGTCGTACACGAGCCAGGAGGAGCACGACTACAGGTTTGAGGTGTTCAAGACCAACATAAGGATGGCCAAGATTCACCAGCGAATGGACCCCACGGCCCAACACGGTATGACTCGGTTCTCCTATTTGACTCCGACCGAGTTCAAGAGAAGCCATTTAGGGCTCAGAGGGCTGAGGCTGCCAGCCAATGCCAACAAGGCGCCGATTCTGCCTTCCAAGAATCTGCCTGAGGATTTTGATTTGAGAAAGCATAGAGCTGTTACTGAAGTCAAAAATTGA

Protein Analysis

248

Amino Acids

27.61

Weight (kDa)

8.87

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 147 - 203 2.1e-14 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 664
AccB7I CCANNNNNTGG 2 cut(s) 80, 550
AciI CCGC 2 cut(s) 194, 372
AclWI GGATC 2 cut(s) 294, 346
AcoI YGGCCR 2 cut(s) 3, 531
AcsI RAATTY 2 cut(s) 291, 458
AcuI CTGAAG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 665
AfaI GTAC 1 cut(s) 473
AfeI AGCGCT 1 cut(s) 168
AfiI CCNNNNNNNGG 3 cut(s) 80, 525, 550
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 3 cut(s) 451, 514, 610
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 4 cut(s) 231, 267, 428, 722
AluI AGCT 4 cut(s) 231, 267, 428, 722
Alw21I GWGCWC 3 cut(s) 233, 435, 492
AlwI GGATC 2 cut(s) 294, 346
Ama87I CYCGRG 1 cut(s) 142
Aor51HI AGCGCT 1 cut(s) 168
AoxI GGCC 3 cut(s) 3, 531, 561
ApeKI GCWGC 2 cut(s) 213, 643
ApoI RAATTY 2 cut(s) 291, 458
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
Asp700I GAANNNNTTC 2 cut(s) 384, 458
AspLEI GCGC 2 cut(s) 169, 667
AspS9I GGNCC 3 cut(s) 197, 553, 562
AsuC2I CCSGG 2 cut(s) 143, 144
AsuHPI GGTGA 3 cut(s) 63, 380, 533
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 2 cut(s) 197, 553
AxyI CCTNAGG 1 cut(s) 693
BalI TGGCCA 2 cut(s) 5, 533
BanI GGYRCC 1 cut(s) 664
BanII GRGCYC 3 cut(s) 143, 233, 630
BauI CACGAG 1 cut(s) 475
Bbv12I GWGCWC 3 cut(s) 233, 435, 492
BbvCI CCTCAGC 1 cut(s) 638
BbvI GCAGC 2 cut(s) 225, 630
BccI CCATC 5 cut(s) 14, 97, 182, 277, 523
BceAI ACGGC 3 cut(s) 199, 424, 576
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 2 cut(s) 143, 144
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 2 cut(s) 170, 668
BglI GCCNNNNNGGC 1 cut(s) 662
BisI GCNGC 3 cut(s) 214, 373, 644
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 3 cut(s) 215, 374, 645
Bme1390I CCNGG 3 cut(s) 143, 144, 482
Bme18I GGWCC 2 cut(s) 197, 553
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 3 cut(s) 197, 553, 562
BmiI GGNNCC 3 cut(s) 198, 555, 666
BmrFI CCNGG 3 cut(s) 143, 144, 482
BmsI GCATC 1 cut(s) 75
Bpu10I CCTNAGC 1 cut(s) 638
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 143, 144
BsaHI GRCGYC 1 cut(s) 665
BsaJI CCNNGG 2 cut(s) 142, 558
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 525, 550
Bse1I ACTGG 1 cut(s) 271
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 2 cut(s) 142, 558
BseGI GGATG 2 cut(s) 90, 534
BseLI CCNNNNNNNGG 3 cut(s) 80, 525, 550
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 5 cut(s) 420, 436, 629, 643, 684
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 225, 630
Bsh1285I CGRYCG 1 cut(s) 603
BshFI GGCC 3 cut(s) 5, 533, 563
BshNI GGYRCC 1 cut(s) 664
BsiEI CGRYCG 1 cut(s) 603
BsiHKAI GWGCWC 3 cut(s) 233, 435, 492
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 2 cut(s) 143, 200
BslI CCNNNNNNNGG 3 cut(s) 80, 525, 550
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 3 cut(s) 5, 533, 563
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 5 cut(s) 143, 233, 435, 492, 630
Bsp143I GATC 3 cut(s) 286, 351, 400
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 2 cut(s) 194, 372
BspANI GGCC 3 cut(s) 5, 533, 563
BspCNI CTCAG 5 cut(s) 421, 435, 630, 642, 685
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 198, 555, 666
BspPI GGATC 2 cut(s) 294, 346
BspT107I GGYRCC 1 cut(s) 664
BsrDI GCAATG 1 cut(s) 154
BsrI ACTGG 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 142, 558
BssMI GATC 3 cut(s) 286, 351, 400
BssNI GRCGYC 1 cut(s) 665
BssSI CACGAG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 3 cut(s) 69, 338, 572
Bst6I CTCTTC 2 cut(s) 308, 314
BstACI GRCGYC 1 cut(s) 665
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 5 cut(s) 422, 429, 629, 638, 693
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 2 cut(s) 90, 534
BstH2I RGCGCY 2 cut(s) 170, 668
BstHHI GCGC 2 cut(s) 169, 667
BstKTI GATC 3 cut(s) 289, 354, 403
BstMBI GATC 3 cut(s) 286, 351, 400
BstMCI CGRYCG 1 cut(s) 603
BstMWI GCNNNNNNNGC 4 cut(s) 634, 643, 662, 673
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 3 cut(s) 141, 142, 480
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 2 cut(s) 225, 630
Bsu36I CCTNAGG 1 cut(s) 693
BsuRI GGCC 3 cut(s) 5, 533, 563
BtgI CCRYGG 1 cut(s) 558
BtgZI GCGATG 3 cut(s) 28, 201, 390
BtsCI GGATG 2 cut(s) 90, 534
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 648
CciI TCATGA 1 cut(s) 403
CfoI GCGC 2 cut(s) 169, 667
Cfr13I GGNCC 3 cut(s) 197, 553, 562
Cfr9I CCCGGG 1 cut(s) 142
Csp6I GTAC 1 cut(s) 472
CviAII CATG 1 cut(s) 404
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 5 cut(s) 422, 429, 629, 638, 693
DinI GGCGCC 1 cut(s) 666
DpnI GATC 3 cut(s) 288, 353, 402
DpnII GATC 3 cut(s) 286, 351, 400
EaeI YGGCCR 2 cut(s) 3, 531
Eam1104I CTCTTC 2 cut(s) 308, 314
EarI CTCTTC 2 cut(s) 308, 314
Ecl136II GAGCTC 1 cut(s) 231
Eco24I GRGCYC 3 cut(s) 143, 233, 630
Eco47I GGWCC 2 cut(s) 197, 553
Eco47III AGCGCT 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 94
Eco81I CCTNAGG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 231
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 480
EcoT38I GRGCYC 3 cut(s) 143, 233, 630
EgeI GGCGCC 1 cut(s) 666
EheI GGCGCC 1 cut(s) 666
FaeI CATG 1 cut(s) 407
FaiI YATR 7 cut(s) 162, 251, 264, 405, 524, 575, 717
FatI CATG 1 cut(s) 403
FauI CCCGC 1 cut(s) 187
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 3 cut(s) 214, 373, 644
FokI GGATG 2 cut(s) 97, 541
FriOI GRGCYC 3 cut(s) 143, 233, 630
Fsp4HI GCNGC 3 cut(s) 214, 373, 644
GlaI GCGC 2 cut(s) 168, 666
GluI GCNGC 3 cut(s) 214, 373, 644
HaeII RGCGCY 2 cut(s) 170, 668
HaeIII GGCC 3 cut(s) 5, 533, 563
HapII CCGG 2 cut(s) 143, 200
HhaI GCGC 2 cut(s) 169, 667
Hin1I GRCGYC 1 cut(s) 665
Hin1II CATG 1 cut(s) 407
Hin6I GCGC 2 cut(s) 167, 665
HinP1I GCGC 2 cut(s) 167, 665
HinfI GANTC 6 cut(s) 134, 538, 577, 595, 670, 685
HpaII CCGG 2 cut(s) 143, 200
HphI GGTGA 3 cut(s) 63, 380, 533
Hpy166II GTNNAC 2 cut(s) 334, 474
Hpy188I TCNGA 3 cut(s) 425, 600, 632
Hpy188III TCNNGA 5 cut(s) 280, 404, 451, 514, 610
Hpy8I GTNNAC 2 cut(s) 334, 474
HpyAV CCTTC 3 cut(s) 362, 395, 687
HpyCH4III ACNGT 3 cut(s) 69, 338, 572
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 4 cut(s) 634, 643, 662, 673
HpyF3I CTNAG 5 cut(s) 422, 429, 629, 638, 693
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 1 cut(s) 665
Hsp92II CATG 1 cut(s) 407
HspAI GCGC 2 cut(s) 167, 665
KasI GGCGCC 1 cut(s) 664
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 3 cut(s) 286, 351, 400
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 2 cut(s) 225, 630
LweI GCATC 1 cut(s) 75
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 69, 724
MalI GATC 3 cut(s) 288, 353, 402
MboI GATC 3 cut(s) 286, 351, 400
MboII GAAGA 3 cut(s) 295, 301, 304
MfeI CAATTG 1 cut(s) 347
MhlI GDGCHC 5 cut(s) 143, 233, 435, 492, 630
MlsI TGGCCA 2 cut(s) 5, 533
MluCI AATT 6 cut(s) 175, 255, 291, 347, 458, 739
MluNI TGGCCA 2 cut(s) 5, 533
Mly113I GGCGCC 1 cut(s) 665
MlyI GAGTC 2 cut(s) 571, 589
MmeI TCCRAC 2 cut(s) 111, 623
MnlI CCTC 8 cut(s) 255, 317, 326, 478, 499, 626, 633, 688
Mox20I TGGCCA 2 cut(s) 5, 533
MroXI GAANNNNTTC 2 cut(s) 384, 458
MscI TGGCCA 2 cut(s) 5, 533
MslI CAYNNNNRTG 2 cut(s) 396, 527
Msp20I TGGCCA 2 cut(s) 5, 533
MspI CCGG 2 cut(s) 143, 200
MspR9I CCNGG 3 cut(s) 143, 144, 482
MunI CAATTG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 482
MwoI GCNNNNNNNGC 4 cut(s) 634, 643, 662, 673
NarI GGCGCC 1 cut(s) 665
NciI CCSGG 2 cut(s) 143, 144
NdeII GATC 3 cut(s) 286, 351, 400
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 3 cut(s) 198, 555, 666
NmuCI GTSAC 1 cut(s) 69
PagI TCATGA 1 cut(s) 403
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 2 cut(s) 384, 458
PfeI GAWTC 4 cut(s) 134, 538, 670, 685
PflMI CCANNNNNTGG 2 cut(s) 80, 550
PkrI GCNGC 3 cut(s) 215, 374, 645
PleI GAGTC 2 cut(s) 571, 589
PluTI GGCGCC 1 cut(s) 668
PpsI GAGTC 2 cut(s) 571, 589
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 480
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 3 cut(s) 198, 555, 666
PspPI GGNCC 3 cut(s) 197, 553, 562
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 2 cut(s) 396, 527
SacI GAGCTC 1 cut(s) 233
SatI GCNGC 3 cut(s) 214, 373, 644
Sau3AI GATC 3 cut(s) 286, 351, 400
Sau96I GGNCC 3 cut(s) 197, 553, 562
SchI GAGTC 2 cut(s) 571, 589
ScrFI CCNGG 3 cut(s) 143, 144, 482
SduI GDGCHC 5 cut(s) 143, 233, 435, 492, 630
SetI ASST 7 cut(s) 120, 233, 269, 430, 503, 510, 724
SfaNI GCATC 1 cut(s) 75
SfcI CTRYAG 1 cut(s) 496
SfoI GGCGCC 1 cut(s) 666
SinI GGWCC 2 cut(s) 197, 553
SmaI CCCGGG 1 cut(s) 144
SmiMI CAYNNNNRTG 2 cut(s) 396, 527
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SrfI GCCCGGGC 1 cut(s) 144
Sse9I AATT 6 cut(s) 175, 255, 291, 347, 458, 739
SsiI CCGC 2 cut(s) 194, 372
SspDI GGCGCC 1 cut(s) 664
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 141, 142, 480
TaaI ACNGT 3 cut(s) 69, 338, 572
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 137, 206, 227, 289
TaqII GACCGA 1 cut(s) 617
TasI AATT 6 cut(s) 175, 255, 291, 347, 458, 739
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 4 cut(s) 134, 538, 670, 685
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 69
TseI GCWGC 2 cut(s) 213, 643
Tsp45I GTSAC 1 cut(s) 69
TspDTI ATGAA 1 cut(s) 393
TspGWI ACGGA 1 cut(s) 344
TspMI CCCGGG 1 cut(s) 142
TspRI CASTG 1 cut(s) 223
Van91I CCANNNNNTGG 2 cut(s) 80, 550
VpaK11BI GGWCC 2 cut(s) 197, 553
XapI RAATTY 2 cut(s) 291, 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmaI CCCGGG 1 cut(s) 142
XmnI GAANNNNTTC 2 cut(s) 384, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.