RLG00000008086

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010557
Physical Location & Seq
Reverse (-)
25454957 .. 25457013
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008086

Sequence Viewer

Length: 663 bp
ATGTCTACTGGTGATTCACCCACTTTCAAATATTTTTCCTGTGGCCATACTCCATGGCCTTCTGATCGGACCGGCCACACCGCATCATCACCTCAACAACATCAACCGCTTTTCTTTCATTTCCGTATGGGAGAGGTGGAGTTTAGATTGTGGAGATATGTTGAGGCTTCTGCTGCATCTTTCAGGCACACAGATAACATGGCAGATCGAGAACTTGCAAGCCACTGGATATACCAAAGGGAGAAATTCATAAAAGCAGATTTCCCAACTCACTGCAGTTATGGACCAGTAATGCTTGAAGACCCTTTCAAATGGCAAGGTGTTATTATGGGGCCTCCAGATTCTCCTTATGAAGAAGGCGTCTTCTTTCTTTCCATCGAGTTACCTGACGACTATCCAACCAAGCCTCCCACCATCAAATTCCTAACCAAGGTTTATCATCCAAACATCGATGAAGATGGCAATATTTACGTTGACATTTTAGAAGAAGATCAATGGAACCCAGTCCAGAACATTGAGAGCCTATTGCTCTCCATATGCTCACTTCTTAATGATCCAAATCCAGTGGACCCTCTCAATCCAAGCAGCAAAGTCTTCAAAACAAACAGACAGGAATTCAACAAATTGGCGAAAGAATGGACTAGGAAATATGCTAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

221

Amino Acids

25.57

Weight (kDa)

5.25

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 87 - 213 3.3e-43 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 81, 107
AclWI GGATC 1 cut(s) 548
AcoI YGGCCR 2 cut(s) 43, 73
AcsI RAATTY 3 cut(s) 245, 419, 614
AcyI GRCGYC 1 cut(s) 360
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 5 cut(s) 28, 299, 310, 598, 619
AlwI GGATC 1 cut(s) 548
AoxI GGCC 4 cut(s) 43, 56, 73, 332
ApeKI GCWGC 2 cut(s) 173, 585
ApoI RAATTY 3 cut(s) 245, 419, 614
AspS9I GGNCC 4 cut(s) 69, 284, 332, 568
AsuHPI GGTGA 3 cut(s) 9, 23, 81
AvaII GGWCC 3 cut(s) 69, 284, 568
BalI TGGCCA 1 cut(s) 45
BbsI GAAGAC 3 cut(s) 306, 355, 586
BbvI GCAGC 2 cut(s) 160, 597
BccI CCATC 3 cut(s) 383, 422, 452
BfaI CTAG 2 cut(s) 642, 654
BfmI CTRYAG 1 cut(s) 274
BisI GCNGC 2 cut(s) 174, 586
BlsI GCNGC 2 cut(s) 175, 587
Bme18I GGWCC 3 cut(s) 69, 284, 568
BmgT120I GGNCC 4 cut(s) 69, 284, 332, 568
BmiI GGNNCC 3 cut(s) 333, 500, 570
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 2 cut(s) 92, 185
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 3 cut(s) 306, 355, 586
BpmI CTGGAG 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 450
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 2 cut(s) 53, 429
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 5 cut(s) 13, 230, 287, 503, 563
Bse8I GATNNNNATC 1 cut(s) 558
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 53, 429
BseGI GGATG 1 cut(s) 439
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 1 cut(s) 430
BseNI ACTGG 5 cut(s) 13, 230, 287, 503, 563
BseXI GCAGC 2 cut(s) 160, 597
BshFI GGCC 4 cut(s) 45, 58, 75, 334
BshVI ATCGAT 1 cut(s) 450
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 430
BsnI GGCC 4 cut(s) 45, 58, 75, 334
Bsp143I GATC 4 cut(s) 64, 205, 490, 553
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 2 cut(s) 81, 107
BspANI GGCC 4 cut(s) 45, 58, 75, 334
BspDI ATCGAT 1 cut(s) 450
BspLI GGNNCC 3 cut(s) 333, 500, 570
BspMAI CTGCAG 1 cut(s) 278
BspPI GGATC 1 cut(s) 548
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 5 cut(s) 13, 230, 287, 503, 563
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 53, 429
BssMI GATC 4 cut(s) 64, 205, 490, 553
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 53, 429
BstACI GRCGYC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 220
BstDSI CCRYGG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 4 cut(s) 67, 208, 493, 556
BstMBI GATC 4 cut(s) 64, 205, 490, 553
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 160, 597
BstV2I GAAGAC 3 cut(s) 306, 355, 586
Bsu15I ATCGAT 1 cut(s) 450
BsuRI GGCC 4 cut(s) 45, 58, 75, 334
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 3 cut(s) 223, 271, 570
Cac8I GCNNGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 4 cut(s) 69, 284, 332, 568
ClaI ATCGAT 1 cut(s) 450
CpoI CGGWCCG 1 cut(s) 69
CseI GACGC 1 cut(s) 349
CspCI CAANNNNNGTGG 2 cut(s) 546, 581
CspI CGGWCCG 1 cut(s) 69
CviAII CATG 2 cut(s) 54, 199
CviJI RGCY 8 cut(s) 45, 58, 75, 167, 222, 334, 406, 522
CviKI_1 RGCY 8 cut(s) 45, 58, 75, 167, 222, 334, 406, 522
DpnI GATC 4 cut(s) 66, 207, 492, 555
DpnII GATC 4 cut(s) 64, 205, 490, 553
EaeI YGGCCR 2 cut(s) 43, 73
Eco130I CCWWGG 2 cut(s) 53, 429
Eco47I GGWCC 3 cut(s) 69, 284, 568
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 614
EcoT14I CCWWGG 2 cut(s) 53, 429
ErhI CCWWGG 2 cut(s) 53, 429
FaeI CATG 2 cut(s) 57, 202
FatI CATG 2 cut(s) 53, 198
FauNDI CATATG 1 cut(s) 536
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 174, 586
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 2 cut(s) 174, 586
FspBI CTAG 2 cut(s) 642, 654
GluI GCNGC 2 cut(s) 174, 586
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 4 cut(s) 45, 58, 75, 334
HapII CCGG 1 cut(s) 72
HgaI GACGC 1 cut(s) 349
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 2 cut(s) 57, 202
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 2 cut(s) 14, 341
HpaII CCGG 1 cut(s) 72
HphI GGTGA 3 cut(s) 9, 23, 81
Hpy166II GTNNAC 3 cut(s) 6, 475, 568
Hpy188I TCNGA 2 cut(s) 64, 69
Hpy188III TCNNGA 3 cut(s) 209, 338, 508
Hpy8I GTNNAC 3 cut(s) 6, 475, 568
HpyAV CCTTC 2 cut(s) 69, 350
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 3 cut(s) 176, 218, 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpySE526I ACGT 1 cut(s) 471
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 2 cut(s) 57, 202
Kzo9I GATC 4 cut(s) 64, 205, 490, 553
Lsp1109I GCAGC 2 cut(s) 160, 597
LweI GCATC 2 cut(s) 92, 185
MaeI CTAG 2 cut(s) 642, 654
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 1 cut(s) 381
MalI GATC 4 cut(s) 66, 207, 492, 555
MboI GATC 4 cut(s) 64, 205, 490, 553
MboII GAAGA 7 cut(s) 311, 355, 365, 467, 497, 500, 586
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 4 cut(s) 245, 419, 614, 623
MluNI TGGCCA 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 422
MnlI CCTC 6 cut(s) 102, 127, 157, 345, 417, 582
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 1 cut(s) 549
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 53
NdeI CATATG 1 cut(s) 536
NdeII GATC 4 cut(s) 64, 205, 490, 553
NlaIII CATG 2 cut(s) 57, 202
NlaIV GGNNCC 3 cut(s) 333, 500, 570
PfeI GAWTC 2 cut(s) 14, 341
PkrI GCNGC 2 cut(s) 175, 587
PspN4I GGNNCC 3 cut(s) 333, 500, 570
PspPI GGNCC 4 cut(s) 69, 284, 332, 568
PstI CTGCAG 1 cut(s) 278
Rsr2I CGGWCCG 1 cut(s) 69
RsrII CGGWCCG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 2 cut(s) 174, 586
Sau3AI GATC 4 cut(s) 64, 205, 490, 553
Sau96I GGNCC 4 cut(s) 69, 284, 332, 568
SetI ASST 6 cut(s) 94, 138, 322, 388, 435, 474
SfaNI GCATC 2 cut(s) 92, 185
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 3 cut(s) 69, 284, 568
Sse9I AATT 4 cut(s) 245, 419, 614, 623
SsiI CCGC 2 cut(s) 81, 107
SspI AATATT 2 cut(s) 32, 466
SspMI CTAG 2 cut(s) 642, 654
StyI CCWWGG 2 cut(s) 53, 429
TaiI ACGT 1 cut(s) 474
TaqI TCGA 3 cut(s) 208, 378, 450
TasI AATT 4 cut(s) 245, 419, 614, 623
TfiI GAWTC 2 cut(s) 14, 341
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 3 cut(s) 230, 278, 570
TseI GCWGC 2 cut(s) 173, 585
TspDTI ATGAA 4 cut(s) 107, 238, 366, 468
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 3 cut(s) 230, 278, 570
VpaK11BI GGWCC 3 cut(s) 69, 284, 568
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 3 cut(s) 245, 419, 614
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 642, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.