Rh4BG191700

protein modification by small protein conjugation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010557
Physical Location & Seq
Forward (+)
33145865 .. 33148961
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG191700.1

Sequence Viewer

Length: 336 bp
ATGGGGCCTCCAGATTCTCCTTATGAAGAAGGCGTCTTCTTTCTTTCCATCGAGTTACCTGATGACTATCCAACCAAGCCTCCCATCATCAAATTCCTAACCAAGGTTTATCATCCAAACATCGATGAAGATGGCAATATTTACATTGACATTTTAGAAGAAGATCAATGGAACCCAGTCCAGACCATTGAGAGCCTATTGCTCTCCATATGCTCACTTCTTAATGATCCAAACCCAGTGGACCCTCTCAATCCAAGCAGCGATGTCTTCAAAACAAACAGACAGGAATTCAACAAAATGGCGAAAGAATGGACTAGGAAATATGCTAGTGAATGA

Protein Analysis

111

Amino Acids

12.82

Weight (kDa)

4.31

Isoelectric Point (pI)

59.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 1 - 104 3.8e-39 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 2 cut(s) 92, 287
AcyI GRCGYC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 2 cut(s) 271, 292
AlwI GGATC 1 cut(s) 221
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 1 cut(s) 258
ApoI RAATTY 2 cut(s) 92, 287
AspS9I GGNCC 2 cut(s) 5, 241
AvaII GGWCC 1 cut(s) 241
BbsI GAAGAC 2 cut(s) 28, 259
BbvI GCAGC 1 cut(s) 270
BccI CCATC 3 cut(s) 56, 92, 125
BfaI CTAG 2 cut(s) 315, 327
BisI GCNGC 1 cut(s) 259
BlsI GCNGC 1 cut(s) 260
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 2 cut(s) 5, 241
BmiI GGNNCC 3 cut(s) 6, 173, 243
BmrI ACTGGG 2 cut(s) 170, 230
BmuI ACTGGG 2 cut(s) 170, 230
BpiI GAAGAC 2 cut(s) 28, 259
Bsa29I ATCGAT 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 176, 236
Bse8I GATNNNNATC 1 cut(s) 66
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 176, 236
BseXI GCAGC 1 cut(s) 270
BshFI GGCC 1 cut(s) 7
BshVI ATCGAT 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 103
BsnI GGCC 1 cut(s) 7
Bsp143I GATC 2 cut(s) 163, 226
BspANI GGCC 1 cut(s) 7
BspDI ATCGAT 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 6, 173, 243
BspPI GGATC 1 cut(s) 221
BsrI ACTGG 2 cut(s) 176, 236
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 163, 226
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 102
BstACI GRCGYC 1 cut(s) 33
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 112
BstKTI GATC 2 cut(s) 166, 229
BstMBI GATC 2 cut(s) 163, 226
BstV1I GCAGC 1 cut(s) 270
BstV2I GAAGAC 2 cut(s) 28, 259
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 1 cut(s) 7
BsuTUI ATCGAT 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 5, 241
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 1 cut(s) 22
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviJI RGCY 3 cut(s) 7, 79, 195
CviKI_1 RGCY 3 cut(s) 7, 79, 195
DpnI GATC 2 cut(s) 165, 228
DpnII GATC 2 cut(s) 163, 226
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaiI YATR 4 cut(s) 24, 209, 211, 324
FauNDI CATATG 1 cut(s) 209
Fnu4HI GCNGC 1 cut(s) 259
FokI GGATG 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 259
FspBI CTAG 2 cut(s) 315, 327
GluI GCNGC 1 cut(s) 259
HaeIII GGCC 1 cut(s) 7
HgaI GACGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 33
HinfI GANTC 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 241
Hpy188III TCNNGA 2 cut(s) 11, 181
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 33
Kzo9I GATC 2 cut(s) 163, 226
LpnPI CCDG 6 cut(s) 24, 72, 189, 194, 249, 269
Lsp1109I GCAGC 1 cut(s) 270
MaeI CTAG 2 cut(s) 315, 327
MaeIII GTNAC 1 cut(s) 54
MalI GATC 2 cut(s) 165, 228
MboI GATC 2 cut(s) 163, 226
MboII GAAGA 6 cut(s) 28, 38, 140, 170, 173, 259
MluCI AATT 2 cut(s) 92, 287
MmeI TCCRAC 1 cut(s) 95
MnlI CCTC 3 cut(s) 18, 90, 255
MseI TTAA 1 cut(s) 222
NdeI CATATG 1 cut(s) 209
NdeII GATC 2 cut(s) 163, 226
NlaIV GGNNCC 3 cut(s) 6, 173, 243
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 260
PspN4I GGNNCC 3 cut(s) 6, 173, 243
PspPI GGNCC 2 cut(s) 5, 241
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 1 cut(s) 259
Sau3AI GATC 2 cut(s) 163, 226
Sau96I GGNCC 2 cut(s) 5, 241
SetI ASST 2 cut(s) 61, 108
SinI GGWCC 1 cut(s) 241
Sse9I AATT 2 cut(s) 92, 287
SspI AATATT 1 cut(s) 139
SspMI CTAG 2 cut(s) 315, 327
StyI CCWWGG 1 cut(s) 102
TaqI TCGA 2 cut(s) 51, 123
TasI AATT 2 cut(s) 92, 287
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 243
TseI GCWGC 1 cut(s) 258
TspDTI ATGAA 2 cut(s) 39, 141
TspRI CASTG 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 241
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 2 cut(s) 92, 287
XspI CTAG 2 cut(s) 315, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.