Rh4DG191200

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010557
Physical Location & Seq
Forward (+)
37533560 .. 37534936
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG191200.1

Sequence Viewer

Length: 465 bp
ATGGCAGATGGAGAACTTGCAAGCCACTGGATATACCAAAGGGAGGGATTCATAAAAGCAGATTTCCCAACTCACTGCAGTTATGGACCAGTAAAGCTTGAAGACCCTTTCAAATGGCAAGGTGTTATTATGGGGCCTCCAGATTCTCCTTATGAAGAAGGCGTCTTCTTTCTTTCCATCGAGTTACCTGACGACTATCCAACCAAGCCTCCCATCATCAAATTCCTAACCAAGGTTTATCATCCAAACATCGATGAAGATGGCAATATTTACGTTGACATTTTAGAAGAAGATCAATGGAACCCAATCCAGACCATTGAGAGCCTATTGCTCTCCATATGCTCACTTCTTAATGATCCCAATCCAGTGGACCCTCGCAATCCAAGCAGCGATGACTTCAAAACAAACAGACAGGAATTCAACAAATTGGCGAAAGAATGGACTAGGATATATGCTAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

154

Amino Acids

17.79

Weight (kDa)

4.45

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 21 - 147 1.7e-42 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 2 cut(s) 221, 416
AcyI GRCGYC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 43, 232
AgsI TTSAA 4 cut(s) 101, 112, 400, 421
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
AlwI GGATC 1 cut(s) 350
AoxI GGCC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 387
ApoI RAATTY 2 cut(s) 221, 416
AspS9I GGNCC 3 cut(s) 86, 134, 370
AvaII GGWCC 2 cut(s) 86, 370
BbsI GAAGAC 2 cut(s) 108, 157
BbvI GCAGC 1 cut(s) 399
BccI CCATC 4 cut(s) 2, 185, 221, 254
BfaI CTAG 2 cut(s) 444, 456
BfmI CTRYAG 1 cut(s) 76
BisI GCNGC 1 cut(s) 388
BlsI GCNGC 1 cut(s) 389
Bme18I GGWCC 2 cut(s) 86, 370
BmgT120I GGNCC 3 cut(s) 86, 134, 370
BmiI GGNNCC 3 cut(s) 135, 302, 372
BpiI GAAGAC 2 cut(s) 108, 157
BpmI CTGGAG 1 cut(s) 123
Bsa29I ATCGAT 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 232
Bse1I ACTGG 3 cut(s) 32, 89, 365
Bse8I GATNNNNATC 1 cut(s) 360
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 1 cut(s) 241
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 2 cut(s) 43, 232
BseNI ACTGG 3 cut(s) 32, 89, 365
BseXI GCAGC 1 cut(s) 399
BshFI GGCC 1 cut(s) 136
BshVI ATCGAT 1 cut(s) 252
BslI CCNNNNNNNGG 2 cut(s) 43, 232
BsnI GGCC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 292, 355
BspANI GGCC 1 cut(s) 136
BspDI ATCGAT 1 cut(s) 252
BspLI GGNNCC 3 cut(s) 135, 302, 372
BspMAI CTGCAG 1 cut(s) 80
BspPI GGATC 1 cut(s) 350
BsrI ACTGG 3 cut(s) 32, 89, 365
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 2 cut(s) 292, 355
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 231
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 1 cut(s) 241
BstKTI GATC 2 cut(s) 295, 358
BstMBI GATC 2 cut(s) 292, 355
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 2 cut(s) 108, 157
BstXI CCANNNNNNTGG 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 1 cut(s) 136
BsuTUI ATCGAT 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 3 cut(s) 25, 73, 372
Cac8I GCNNGC 1 cut(s) 22
Cfr13I GGNCC 3 cut(s) 86, 134, 370
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 1 cut(s) 151
CviJI RGCY 5 cut(s) 24, 97, 136, 208, 324
CviKI_1 RGCY 5 cut(s) 24, 97, 136, 208, 324
DpnI GATC 2 cut(s) 294, 357
DpnII GATC 2 cut(s) 292, 355
Eco130I CCWWGG 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 86, 370
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
FaiI YATR 9 cut(s) 34, 53, 84, 131, 153, 338, 340, 451, 453
FauNDI CATATG 1 cut(s) 338
Fnu4HI GCNGC 1 cut(s) 388
FokI GGATG 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 388
FspBI CTAG 2 cut(s) 444, 456
GluI GCNGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 1 cut(s) 136
HgaI GACGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 2 cut(s) 48, 143
Hpy166II GTNNAC 2 cut(s) 277, 370
Hpy188III TCNNGA 2 cut(s) 140, 310
Hpy8I GTNNAC 2 cut(s) 277, 370
HpyAV CCTTC 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 20, 78
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 292, 355
LpnPI CCDG 7 cut(s) 13, 102, 153, 201, 323, 378, 398
Lsp1109I GCAGC 1 cut(s) 399
MaeI CTAG 2 cut(s) 444, 456
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 183
MalI GATC 2 cut(s) 294, 357
MboI GATC 2 cut(s) 292, 355
MboII GAAGA 6 cut(s) 113, 157, 167, 269, 299, 302
MluCI AATT 3 cut(s) 221, 416, 425
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 4 cut(s) 37, 147, 219, 384
MseI TTAA 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 384
NdeI CATATG 1 cut(s) 338
NdeII GATC 2 cut(s) 292, 355
NlaIV GGNNCC 3 cut(s) 135, 302, 372
PfeI GAWTC 2 cut(s) 48, 143
PkrI GCNGC 1 cut(s) 389
PspN4I GGNNCC 3 cut(s) 135, 302, 372
PspPI GGNCC 3 cut(s) 86, 134, 370
PstI CTGCAG 1 cut(s) 80
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 388
Sau3AI GATC 2 cut(s) 292, 355
Sau96I GGNCC 3 cut(s) 86, 134, 370
SetI ASST 5 cut(s) 99, 124, 190, 237, 276
SfcI CTRYAG 1 cut(s) 76
SinI GGWCC 2 cut(s) 86, 370
Sse9I AATT 3 cut(s) 221, 416, 425
SspI AATATT 1 cut(s) 268
SspMI CTAG 2 cut(s) 444, 456
StyI CCWWGG 1 cut(s) 231
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 180, 252
TasI AATT 3 cut(s) 221, 416, 425
TfiI GAWTC 2 cut(s) 48, 143
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 3 cut(s) 32, 80, 372
TseI GCWGC 1 cut(s) 387
TspDTI ATGAA 3 cut(s) 40, 168, 270
TspRI CASTG 3 cut(s) 32, 80, 372
VpaK11BI GGWCC 2 cut(s) 86, 370
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 2 cut(s) 221, 416
XspI CTAG 2 cut(s) 444, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.