Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004066

Physical Location & Seq

Genomic Coordinates Forward (+)

6304033 .. 6305867

Gene Structure 1835 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011040

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGGAAAACCCAGACAAGACAAAAGGCACAAGATCACCACCACCTCCGCCGCCGCAACAACCAGACCCCAAGAAGCTCAAAATGTCCACCACCACCACCGACGACGAAGAAACCACCACCACCCAAAAGCAACGCATCAAACGCCGCAAGGTCGCAATCTTCTTCGCCTATTGCGGCGTCGGATACCAAGGAATGCAAAAGAACCCCGGCGCCAAAACCATCGAAGGCGACCTCGAAGAAGCGCTCTACCTCTCCGGCGCGGTTCCCGAGCAGGACCGCAACAACCCTAAGCGTTACGACTGGGCCCGCTCGGCCCGTACCGACAAGGGCGTCAGCGCCGTGGGCCAGGTCGTATCGGGCCGGTTCTATGTCGACCCGCCGGGGTTCGTGGACCGCCTCAATTCGAACCTCTCCCCGCAAATTCGGATTTTCGGGTACAAGCGCGTGACGGCGTCGTTTAATGCGAAGAAGTTCTGTGATCGGAGGAGGTACGTTTATCTTATACCTGTGTTTGCTCTTGATCCTAGTGCCCATAGGGATAGAGAGAGTGTTAAGGCGAGTTTGGGGTCTGATGAGGAGTTTGTGAAGTGCTTGGAGTGCTCCGATAGGGGCCGGAAAGTCGGTGGATTGATGGGTAAGCGTACTTATGAATTGAGAGGTGATAGTTCGAATTCGGAATCTGTAAATGGAATGAGCACAAATGATGATTCTCATATGAAAAACGACATTGCCAATGGTATTGGGGTTTCTGAAGTTGAGGTTGAAGATAATGTAGTGGTTGCCAATGCGGATATGAACTCTGAGGTCAATGGAGAAGAGAAGAAGGTCGTGAAGGGAGGTGGGTTTTGTTATGGTGAGAGGGACAGGGAAAGGTTTAATAGAATCTTGAGTTGTTACCAGGGTACTCACAACTTCCATAACTTCACCACTAGAACTAAGGCTGAAGACCCGGCTGCAACGAGATACATTATTTCGTTCACTGCAAATACCACGGTTACAGTTGAGGGTATTGAATTTGTGAAGTGTGAGGTCGTGGGGCAGAGCTTCATGCTTCATCAGATTAGGAAGTTAATTGGGCTGGCAGTGGCAATCATGAGGAACTGTGCTCCTGAGTCATTGTTAGAAAGAGCTTTGCAAAAGGATGTTAATGTCAATGTGCCTACGGCTCCTGAGGTTGGTTTGTACTTGGATGAGTGCTTCTTCTCATCATATAACCAGAAATGGGGAGACACTCATGAAGAAGTGTCGATAAAAGACTATGAAGCAGAGGCAGAAGCTTTCAAAATGAAGCATATTTACACTCATATTGGAGCGACTGAGCATAAAGAAGGTGTTGTGGGTCTCTGGTTGCACTCTCTTAACCACCGCAATTATCCGGATTTAGGTGCTGACAACAATGGAGACACCAGCATCGAAAGGACTGCTGACATCTCCAGTGCTGACAACAATGGGGATACCAGCAACGGAAACACTGCATATATCGCCAGTGCTGACAACAATGGAGACACCAGCAACGGAAAAACTGCTGATATCTCCAGTTCTGACAACAATGGAGACACCGGCAACGGAAAGACTGCTGATATCTTCAGTGCTGACAACAACGGAGACACCAGCATTGAAAAGACCGCCGATATCTCCAGTGCTGACAACAATGGGGATACCAGCAATGGAAAGACTGCGTATATCGCCAGTGCTGACAACAATGGAGACACCAGCAAGGCTGGTATCTCCAATGTCGCCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0000959 GO:0000963 GO:0001522 GO:0002153 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016556 GO:0016853 GO:0016866 GO:0031119 GO:0031974 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070525 GO:0070900 GO:0070902 GO:0071704 GO:0090304 GO:0090646 GO:0097159 GO:0140053 GO:1900864 GO:1901360 GO:1901363 GO:1990481

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_1

Protein Analysis

582

Amino Acids

63.6

Weight (kDa)

5.37

Isoelectric Point (pI)

31.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_1	PF01416	299 - 401	4.2e-12	tRNA pseudouridine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20370 AT1G76120 AT1G76120
fragaria_vesca	FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_5g07491
malus_domestica	MD15G1005300.v1.1 MD15G1005400.v1.1
prunus_persica	Prupe.1G357900_v2.0.a1
pyrus_communis	pycom08g00460 pycom15g00380 pycom15g00390
rosa_chinensis	RchiOBHm_Chr6g0303831
rosa_laevigata	RLG00000011040
rosa_multiflora	Rmu_co8307379.1_g000001 Rmu_sc0012878.1_g000001 Rmu_sc0022895.1_g000001
rosa_roxburghii	Rroxscaffold_7G00164370
rosa_rugosa	Rorug06G0325200
rosa_samantha	Rh6AG439500 Rh6BG463500 Rh6CG451800 Rh6DG438700
rosa_wichuraiana	Rw6G038050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	329
AccB1I	GGYRCC	1 cut(s)	209
AccBSI	CCGCTC	1 cut(s)	309
AccI	GTMKAC	1 cut(s)	372
AccII	CGCG	2 cut(s)	260, 444
AccIII	TCCGGA	1 cut(s)	1375
AclWI	GGATC	1 cut(s)	515
AcsI	RAATTY	3 cut(s)	420, 670, 1013
AcuI	CTGAAG	3 cut(s)	771, 963, 1570
AcyI	GRCGYC	4 cut(s)	177, 210, 330, 452
AfaI	GTAC	6 cut(s)	319, 437, 491, 643, 904, 1184
AfeI	AGCGCT	1 cut(s)	243
AfiI	CCNNNNNNNGG	3 cut(s)	1175, 1222, 1382
AgsI	TTSAA	4 cut(s)	764, 1013, 1282, 1619
AjnI	CCWGG	2 cut(s)	345, 897
AluBI	AGCT	4 cut(s)	76, 1044, 1130, 1277
AluI	AGCT	4 cut(s)	76, 1044, 1130, 1277
Alw21I	GWGCWC	3 cut(s)	602, 698, 1108
Alw26I	GTCTC	7 cut(s)	1221, 1346, 1395, 1497, 1548, 1599, 1701
AlwI	GGATC	1 cut(s)	515
Ama87I	CYCGRG	1 cut(s)	266
Aor13HI	TCCGGA	1 cut(s)	1375
Aor51HI	AGCGCT	1 cut(s)	243
AoxI	GGCC	5 cut(s)	303, 312, 343, 358, 610
ApaI	GGGCCC	1 cut(s)	307
ApeKI	GCWGC	1 cut(s)	953
ApoI	RAATTY	3 cut(s)	420, 670, 1013
Asp700I	GAANNNNTTC	1 cut(s)	470
AspLEI	GCGC	5 cut(s)	212, 244, 260, 338, 444
AspS9I	GGNCC	8 cut(s)	274, 303, 304, 313, 343, 358, 391, 610
AsuC2I	CCSGG	3 cut(s)	207, 381, 950
AsuHPI	GGTGA	4 cut(s)	27, 671, 866, 916
AsuII	TTCGAA	2 cut(s)	404, 668
AvaI	CYCGRG	1 cut(s)	266
AvaII	GGWCC	2 cut(s)	274, 391
AxyI	CCTNAGG	1 cut(s)	1170
BaeGI	GKGCMC	2 cut(s)	307, 532
BanI	GGYRCC	1 cut(s)	209
BanII	GRGCYC	1 cut(s)	307
BarI	GAAGNNNNNNTAC	2 cut(s)	1280, 1312
BbsI	GAAGAC	1 cut(s)	951
Bbv12I	GWGCWC	3 cut(s)	602, 698, 1108
BbvI	GCAGC	1 cut(s)	940
BccI	CCATC	2 cut(s)	227, 625
BceAI	ACGGC	3 cut(s)	323, 465, 1179
BcgI	CGANNNNNNTGC	2 cut(s)	1403, 1437
BciT130I	CCWGG	2 cut(s)	347, 899
BciVI	GTATCC	3 cut(s)	176, 1447, 1651
BcnI	CCSGG	3 cut(s)	207, 381, 950
BcoDI	GTCTC	7 cut(s)	1221, 1346, 1395, 1497, 1548, 1599, 1701
BfaI	CTAG	2 cut(s)	525, 930
BfoI	RGCGCY	3 cut(s)	213, 245, 339
BfuI	GTATCC	3 cut(s)	176, 1447, 1651
BglI	GCCNNNNNGGC	1 cut(s)	311
BisI	GCNGC	5 cut(s)	50, 53, 145, 175, 954
BlsI	GCNGC	5 cut(s)	51, 54, 146, 176, 955
Bme1390I	CCNGG	5 cut(s)	207, 347, 381, 899, 950
Bme18I	GGWCC	2 cut(s)	274, 391
BmeT110I	CYCGRG	1 cut(s)	266
BmgT120I	GGNCC	8 cut(s)	274, 303, 304, 313, 343, 358, 391, 610
BmiI	GGNNCC	5 cut(s)	211, 264, 305, 611, 1167
BmrFI	CCNGG	5 cut(s)	207, 347, 381, 899, 950
BmrI	ACTGGG	2 cut(s)	310, 1735
BmsI	GCATC	2 cut(s)	144, 1419
BmuI	ACTGGG	2 cut(s)	310, 1735
BpiI	GAAGAC	1 cut(s)	951
BpmI	CTGGAG	3 cut(s)	1417, 1519, 1621
Bpu10I	CCTNAGC	1 cut(s)	288
Bpu14I	TTCGAA	2 cut(s)	404, 668
BpuEI	CTTGAG	1 cut(s)	907
BpuMI	CCSGG	3 cut(s)	207, 381, 950
BsaHI	GRCGYC	4 cut(s)	177, 210, 330, 452
BsaI	GGTCTC	1 cut(s)	1346
BsaJI	CCNNGG	6 cut(s)	187, 205, 339, 380, 898, 990
BsaWI	WCCGGW	1 cut(s)	1375
Bsc4I	CCNNNNNNNGG	3 cut(s)	1175, 1222, 1382
Bse118I	RCCGGY	2 cut(s)	360, 1559
Bse1I	ACTGG	7 cut(s)	305, 1434, 1485, 1536, 1638, 1689, 1741
Bse21I	CCTNAGG	1 cut(s)	1170
Bse3DI	GCAATG	2 cut(s)	726, 1672
BseAI	TCCGGA	1 cut(s)	1375
BseBI	CCWGG	2 cut(s)	347, 899
BseDI	CCNNGG	6 cut(s)	187, 205, 339, 380, 898, 990
BseGI	GGATG	2 cut(s)	1147, 1195
BseLI	CCNNNNNNNGG	3 cut(s)	1175, 1222, 1382
BseMI	GCAATG	2 cut(s)	726, 1672
BseMII	CTCAG	4 cut(s)	792, 1101, 1161, 1308
BseNI	ACTGG	7 cut(s)	305, 1434, 1485, 1536, 1638, 1689, 1741
BseRI	GAGGAG	2 cut(s)	499, 590
BseSI	GKGCMC	2 cut(s)	307, 532
BseXI	GCAGC	1 cut(s)	940
Bsh1236I	CGCG	2 cut(s)	260, 444
BshFI	GGCC	5 cut(s)	305, 314, 345, 360, 612
BshNI	GGYRCC	1 cut(s)	209
BsiHKAI	GWGCWC	3 cut(s)	602, 698, 1108
BsiHKCI	CYCGRG	1 cut(s)	266
BsiSI	CCGG	8 cut(s)	207, 255, 361, 380, 613, 950, 1376, 1560
BslFI	GGGAC	1 cut(s)	875
BslI	CCNNNNNNNGG	3 cut(s)	1175, 1222, 1382
BsmAI	GTCTC	7 cut(s)	1221, 1346, 1395, 1497, 1548, 1599, 1701
BsmFI	GGGAC	1 cut(s)	875
BsmI	GAATGC	1 cut(s)	198
BsnI	GGCC	5 cut(s)	305, 314, 345, 360, 612
Bso31I	GGTCTC	1 cut(s)	1346
BsoBI	CYCGRG	1 cut(s)	266
Bsp119I	TTCGAA	2 cut(s)	404, 668
Bsp120I	GGGCCC	1 cut(s)	303
Bsp1286I	GDGCHC	5 cut(s)	307, 532, 602, 698, 1108
Bsp13I	TCCGGA	1 cut(s)	1375
Bsp143I	GATC	3 cut(s)	32, 478, 520
BspANI	GGCC	5 cut(s)	305, 314, 345, 360, 612
BspCNI	CTCAG	4 cut(s)	793, 1102, 1162, 1309
BspEI	TCCGGA	1 cut(s)	1375
BspFNI	CGCG	2 cut(s)	260, 444
BspHI	TCATGA	2 cut(s)	1092, 1234
BspLI	GGNNCC	5 cut(s)	211, 264, 305, 611, 1167
BspPI	GGATC	1 cut(s)	515
BspT104I	TTCGAA	2 cut(s)	404, 668
BspT107I	GGYRCC	1 cut(s)	209
BspTNI	GGTCTC	1 cut(s)	1346
BsrBI	CCGCTC	1 cut(s)	309
BsrDI	GCAATG	2 cut(s)	726, 1672
BsrFI	RCCGGY	2 cut(s)	360, 1559
BsrI	ACTGG	7 cut(s)	305, 1434, 1485, 1536, 1638, 1689, 1741
BssAI	RCCGGY	2 cut(s)	360, 1559
BssECI	CCNNGG	6 cut(s)	187, 205, 339, 380, 898, 990
BssMI	GATC	3 cut(s)	32, 478, 520
BssNI	GRCGYC	4 cut(s)	177, 210, 330, 452
BssT1I	CCWWGG	1 cut(s)	187
Bst2UI	CCWGG	2 cut(s)	347, 899
Bst4CI	ACNGT	3 cut(s)	994, 1000, 1103
Bst6I	CTCTTC	1 cut(s)	810
BstACI	GRCGYC	4 cut(s)	177, 210, 330, 452
BstBI	TTCGAA	2 cut(s)	404, 668
BstC8I	GCNNGC	2 cut(s)	307, 1080
BstDEI	CTNAG	6 cut(s)	288, 801, 936, 1110, 1170, 1317
BstDSI	CCRYGG	2 cut(s)	339, 990
BstF5I	GGATG	2 cut(s)	1147, 1195
BstFNI	CGCG	2 cut(s)	260, 444
BstH2I	RGCGCY	3 cut(s)	213, 245, 339
BstHHI	GCGC	5 cut(s)	212, 244, 260, 338, 444
BstKTI	GATC	3 cut(s)	35, 481, 523
BstMAI	GTCTC	7 cut(s)	1221, 1346, 1395, 1497, 1548, 1599, 1701
BstMBI	GATC	3 cut(s)	32, 478, 520
BstMWI	GCNNNNNNNGC	6 cut(s)	141, 311, 342, 597, 1481, 1685
BstNI	CCWGG	2 cut(s)	347, 899
BstSCI	CCNGG	5 cut(s)	205, 345, 379, 897, 948
BstSLI	GKGCMC	2 cut(s)	307, 532
BstUI	CGCG	2 cut(s)	260, 444
BstV1I	GCAGC	1 cut(s)	940
BstV2I	GAAGAC	1 cut(s)	951
Bsu36I	CCTNAGG	1 cut(s)	1170
BsuI	GTATCC	3 cut(s)	176, 1447, 1651
BsuRI	GGCC	5 cut(s)	305, 314, 345, 360, 612
BtgI	CCRYGG	2 cut(s)	339, 990
BtsCI	GGATG	2 cut(s)	1147, 1195
BtsI	GCAGTG	3 cut(s)	978, 1089, 1470
BtsIMutI	CAGTG	8 cut(s)	978, 1089, 1441, 1470, 1492, 1594, 1645, 1696
Cac8I	GCNNGC	2 cut(s)	307, 1080
CciI	TCATGA	2 cut(s)	1092, 1234
CfoI	GCGC	5 cut(s)	212, 244, 260, 338, 444
Cfr10I	RCCGGY	2 cut(s)	360, 1559
Cfr13I	GGNCC	8 cut(s)	274, 303, 304, 313, 343, 358, 391, 610
CseI	GACGC	3 cut(s)	166, 319, 441
Csp6I	GTAC	6 cut(s)	318, 436, 490, 642, 903, 1183
CviAII	CATG	3 cut(s)	1048, 1093, 1235
CviQI	GTAC	6 cut(s)	318, 436, 490, 642, 903, 1183
DdeI	CTNAG	6 cut(s)	288, 801, 936, 1110, 1170, 1317
DinI	GGCGCC	1 cut(s)	211
DpnI	GATC	3 cut(s)	34, 480, 522
DpnII	GATC	3 cut(s)	32, 478, 520
DrdI	GACNNNNNNGTC	1 cut(s)	329
DseDI	GACNNNNNNGTC	1 cut(s)	329
Eam1104I	CTCTTC	1 cut(s)	810
EarI	CTCTTC	1 cut(s)	810
EciI	GGCGGA	1 cut(s)	36
Eco130I	CCWWGG	1 cut(s)	187
Eco24I	GRGCYC	1 cut(s)	307
Eco31I	GGTCTC	1 cut(s)	1346
Eco32I	GATATC	3 cut(s)	1531, 1582, 1633
Eco47I	GGWCC	2 cut(s)	274, 391
Eco47III	AGCGCT	1 cut(s)	243
Eco57I	CTGAAG	3 cut(s)	771, 963, 1570
Eco81I	CCTNAGG	1 cut(s)	1170
Eco88I	CYCGRG	1 cut(s)	266
EcoRI	GAATTC	1 cut(s)	670
EcoRII	CCWGG	2 cut(s)	345, 897
EcoRV	GATATC	3 cut(s)	1531, 1582, 1633
EcoT14I	CCWWGG	1 cut(s)	187
EcoT38I	GRGCYC	1 cut(s)	307
EgeI	GGCGCC	1 cut(s)	211
EheI	GGCGCC	1 cut(s)	211
ErhI	CCWWGG	1 cut(s)	187
FaeI	CATG	3 cut(s)	1051, 1096, 1238
FaqI	GGGAC	1 cut(s)	875
FatI	CATG	3 cut(s)	1047, 1092, 1234
FauI	CCCGC	3 cut(s)	314, 384, 423
FauNDI	CATATG	1 cut(s)	714
FblI	GTMKAC	1 cut(s)	372
Fnu4HI	GCNGC	5 cut(s)	50, 53, 145, 175, 954
FokI	GGATG	2 cut(s)	1154, 1202
FriOI	GRGCYC	1 cut(s)	307
Fsp4HI	GCNGC	5 cut(s)	50, 53, 145, 175, 954
FspBI	CTAG	2 cut(s)	525, 930
GlaI	GCGC	5 cut(s)	211, 243, 259, 337, 443
GluI	GCNGC	5 cut(s)	50, 53, 145, 175, 954
GsuI	CTGGAG	3 cut(s)	1417, 1519, 1621
HaeII	RGCGCY	3 cut(s)	213, 245, 339
HaeIII	GGCC	5 cut(s)	305, 314, 345, 360, 612
HapII	CCGG	8 cut(s)	207, 255, 361, 380, 613, 950, 1376, 1560
HgaI	GACGC	3 cut(s)	166, 319, 441
HhaI	GCGC	5 cut(s)	212, 244, 260, 338, 444
Hin1I	GRCGYC	4 cut(s)	177, 210, 330, 452
Hin1II	CATG	3 cut(s)	1051, 1096, 1238
Hin6I	GCGC	5 cut(s)	210, 242, 258, 336, 442
HinP1I	GCGC	5 cut(s)	210, 242, 258, 336, 442
HincII	GTYRAC	1 cut(s)	373
HindII	GTYRAC	1 cut(s)	373
HindIII	AAGCTT	1 cut(s)	1275
HinfI	GANTC	4 cut(s)	677, 707, 882, 1112
HpaII	CCGG	8 cut(s)	207, 255, 361, 380, 613, 950, 1376, 1560
HphI	GGTGA	4 cut(s)	27, 671, 866, 916
Hpy166II	GTNNAC	4 cut(s)	87, 373, 391, 978
Hpy188III	TCNNGA	9 cut(s)	266, 518, 829, 886, 1093, 1109, 1169, 1235, 1376
Hpy8I	GTNNAC	4 cut(s)	87, 373, 391, 978
Hpy99I	CGWCG	4 cut(s)	104, 107, 182, 457
HpyAV	CCTTC	4 cut(s)	218, 817, 826, 1322
HpyCH4III	ACNGT	3 cut(s)	994, 1000, 1103
HpyCH4IV	ACGT	1 cut(s)	492
HpyCH4V	TGCA	6 cut(s)	196, 956, 983, 1135, 1351, 1475
HpyF10VI	GCNNNNNNNGC	6 cut(s)	141, 311, 342, 597, 1481, 1685
HpyF3I	CTNAG	6 cut(s)	288, 801, 936, 1110, 1170, 1317
HpySE526I	ACGT	1 cut(s)	492
Hsp92I	GRCGYC	4 cut(s)	177, 210, 330, 452
Hsp92II	CATG	3 cut(s)	1051, 1096, 1238
HspAI	GCGC	5 cut(s)	210, 242, 258, 336, 442
KasI	GGCGCC	1 cut(s)	209
Kpn2I	TCCGGA	1 cut(s)	1375
Kzo9I	GATC	3 cut(s)	32, 478, 520
LmnI	GCTCC	4 cut(s)	605, 1111, 1171, 1310
Lsp1109I	GCAGC	1 cut(s)	940
LweI	GCATC	2 cut(s)	144, 1419
MaeI	CTAG	2 cut(s)	525, 930
MaeII	ACGT	1 cut(s)	492
MaeIII	GTNAC	4 cut(s)	293, 445, 893, 994
MalI	GATC	3 cut(s)	34, 480, 522
MbiI	CCGCTC	1 cut(s)	309
MboI	GATC	3 cut(s)	32, 478, 520
MhlI	GDGCHC	5 cut(s)	307, 532, 602, 698, 1108
MluCI	AATT	7 cut(s)	400, 420, 650, 670, 1013, 1071, 1369
Mly113I	GGCGCC	1 cut(s)	210
MlyI	GAGTC	1 cut(s)	1121
MmeI	TCCRAC	1 cut(s)	160
MroI	TCCGGA	1 cut(s)	1375
MroXI	GAANNNNTTC	1 cut(s)	470
MseI	TTAA	6 cut(s)	459, 552, 876, 1070, 1146, 1359
MspI	CCGG	8 cut(s)	207, 255, 361, 380, 613, 950, 1376, 1560
MspR9I	CCNGG	5 cut(s)	207, 347, 381, 899, 950
Mva1269I	GAATGC	1 cut(s)	198
MvaI	CCWGG	2 cut(s)	347, 899
MvnI	CGCG	2 cut(s)	260, 444
MwoI	GCNNNNNNNGC	6 cut(s)	141, 311, 342, 597, 1481, 1685
NarI	GGCGCC	1 cut(s)	210
NciI	CCSGG	3 cut(s)	207, 381, 950
NdeI	CATATG	1 cut(s)	714
NdeII	GATC	3 cut(s)	32, 478, 520
NlaIII	CATG	3 cut(s)	1051, 1096, 1238
NlaIV	GGNNCC	5 cut(s)	211, 264, 305, 611, 1167
NmeAIII	GCCGAG	1 cut(s)	290
NmuCI	GTSAC	1 cut(s)	445
NspV	TTCGAA	2 cut(s)	404, 668
PagI	TCATGA	2 cut(s)	1092, 1234
PcsI	WCGNNNNNNNCGW	1 cut(s)	461
PctI	GAATGC	1 cut(s)	198
PdmI	GAANNNNTTC	1 cut(s)	470
PfeI	GAWTC	3 cut(s)	677, 707, 882
PflFI	GACNNNGTC	1 cut(s)	451
PkrI	GCNGC	5 cut(s)	51, 54, 146, 176, 955
PleI	GAGTC	1 cut(s)	1120
PluTI	GGCGCC	1 cut(s)	213
PpsI	GAGTC	1 cut(s)	1120
Psp6I	CCWGG	2 cut(s)	345, 897
PspGI	CCWGG	2 cut(s)	345, 897
PspN4I	GGNNCC	5 cut(s)	211, 264, 305, 611, 1167
PspOMI	GGGCCC	1 cut(s)	303
PspPI	GGNCC	8 cut(s)	274, 303, 304, 313, 343, 358, 391, 610
PsyI	GACNNNGTC	1 cut(s)	451
RsaI	GTAC	6 cut(s)	319, 437, 491, 643, 904, 1184
RsaNI	GTAC	6 cut(s)	318, 436, 490, 642, 903, 1183
SalI	GTCGAC	1 cut(s)	371
SaqAI	TTAA	6 cut(s)	459, 552, 876, 1070, 1146, 1359
SatI	GCNGC	5 cut(s)	50, 53, 145, 175, 954
Sau3AI	GATC	3 cut(s)	32, 478, 520
Sau96I	GGNCC	8 cut(s)	274, 303, 304, 313, 343, 358, 391, 610
SchI	GAGTC	1 cut(s)	1121
ScrFI	CCNGG	5 cut(s)	207, 347, 381, 899, 950
SduI	GDGCHC	5 cut(s)	307, 532, 602, 698, 1108
SfaNI	GCATC	2 cut(s)	144, 1419
SfiI	GGCCNNNNNGGCC	1 cut(s)	311
SfoI	GGCGCC	1 cut(s)	211
SfuI	TTCGAA	2 cut(s)	404, 668
SinI	GGWCC	2 cut(s)	274, 391
SmlI	CTYRAG	1 cut(s)	886
SmoI	CTYRAG	1 cut(s)	886
Sse9I	AATT	7 cut(s)	400, 420, 650, 670, 1013, 1071, 1369
SspDI	GGCGCC	1 cut(s)	209
SspMI	CTAG	2 cut(s)	525, 930
StyD4I	CCNGG	5 cut(s)	205, 345, 379, 897, 948
StyI	CCWWGG	1 cut(s)	187
TaaI	ACNGT	3 cut(s)	994, 1000, 1103
TaiI	ACGT	1 cut(s)	495
TaqI	TCGA	7 cut(s)	222, 234, 372, 404, 668, 1247, 1413
TasI	AATT	7 cut(s)	400, 420, 650, 670, 1013, 1071, 1369
TatI	WGTACW	1 cut(s)	1182
TauI	GCSGC	4 cut(s)	52, 55, 147, 177
TfiI	GAWTC	3 cut(s)	677, 707, 882
Tru1I	TTAA	6 cut(s)	459, 552, 876, 1070, 1146, 1359
Tru9I	TTAA	6 cut(s)	459, 552, 876, 1070, 1146, 1359
TscAI	CASTG	8 cut(s)	985, 1089, 1441, 1477, 1492, 1594, 1645, 1696
TseFI	GTSAC	1 cut(s)	445
TseI	GCWGC	1 cut(s)	953
Tsp45I	GTSAC	1 cut(s)	445
TspDTI	ATGAA	8 cut(s)	663, 731, 809, 1036, 1043, 1251, 1275, 1301
TspGWI	ACGGA	4 cut(s)	1479, 1530, 1581, 1617
TspRI	CASTG	8 cut(s)	985, 1089, 1441, 1477, 1492, 1594, 1645, 1696
Tth111I	GACNNNGTC	1 cut(s)	451
VpaK11BI	GGWCC	2 cut(s)	274, 391
XapI	RAATTY	3 cut(s)	420, 670, 1013
XmiI	GTMKAC	1 cut(s)	372
XmnI	GAANNNNTTC	1 cut(s)	470
XspI	CTAG	2 cut(s)	525, 930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.