Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004066

Physical Location & Seq

Genomic Coordinates Reverse (-)

62260163 .. 62262097

Gene Structure 1935 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G038050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGAAAACCCAGACAACACAAAAGCCACAAGATCACCACCACCTCCGCCGCCGCAACAACCAGAACCCAAGAAGCTCAAAATGTCCACCACCACCACCGACGACGAAGAAACAGCCGCCACCCAAAAGCAACGCATCAAACGCCGCAAGGTCGCAATCTTCTTCGCCTACTGCGGCGTCGGATACCAAGGAATGCAAAAGAACCCCGGCGCCAAAACCATCGAAGGCGACCTCGAAGAAGCCCTCTACCTCTCCGGCGCCGTTCCCGAACAGGACCGCAACAACCCTAAGCGTTATGACTGGGCCCGCTCGGCCCGTACCGACAAGGGCGTCAGCGCCGTGGGCCAGGTCGTATCGGGCCGGTTCTATGTCGACCCGCCGGGGTTCGTGGACCGCCTCAATTCGAACCTCTCCCCGCAAATTCGGATTTTCGGGTACAAGCGCGTGACGGCGTCGTTTAATGCGAAGAAGTTCTGTGATCGGAGGAGGTACGTTTATCTTATACCTGTGTTTGCTCTTGATCCTAGTGCCCATAGGGATAGAGAGAGTGTTAAGGCGAGTTTGGGGTCTGATGAGGAGTTTGTGAAGTGCTTGGAGTGCTCCGATAGGGGGCGGAAAGTCGGTGGATTGATGGGTAAGCGCACTTATGAATTGAGAGGTGATAGTTCGAATTCGGAATCGGTAAATGGAGGAATTAGCACAAATGATGATTCTCATATGAAAAACGACATTGCCAATGATATTGGGGTTTCTGAAGTTGAGGTGGTTGCCAATGCGGATATGAACTCTGAGGTCAATGGAGAAGGCAAGAAGGCTGTGAAGGGAAGTGGGTTTTGTTATGGTGAGAAGGAGAAGGAAAGGTTTAATAGAATCTTGAGTTGTTACCAGGGTACTCACAACTTCCATAACTTCACCACTAGAACTAAGGCTGAAGACCCTGCTGCAACGAGATACATTATTTCGTTCACTGCAAATACCACGGTTACAGTTGAGGGTATTGAATTTGTGAAGTGTGAGGTCGTGGGGCAGAGCTTCATGCTCCATCAGATTAGAAAGTTAATTGGGCTGGCAGTGGCAATCATGAGGAACTGTGCTCCGGAGTCATTGTTAGAAAGAGCTTTGCAAAAGGATGTTAACGTCAATGTGCCTACGGCTCCTGAGGTTGGTTTGTACTTGGATGAGTGCTTCTTCTCATCTTATAACCAGAAATGGGGAGACACTCATGAAGAAGTGTCGATGAAAGACTATGAAGCAGAGGCAGAAGCTTTCAAAATGAAGCATATTTACACTCATATTGGAGCCACTGAGCATAAAGAAGGTGTTGTGGGGCTCTGGTTGCACTCTCTCAACCACCGCAATTATCCGGATTTAGGTGCTGACAACAATGGAGACACCAGCATCGAAAGGACTGCTGATATCTCCAGTGCTGACAACAATGGGGATACTAGCAATGGAAAGACTGCATATATCGCCGGTGCTGACAACAATGGAGACACCAGCAACGGAAAGACTGCCAATATCTCCAGTGCTGACAACAATGGAGACACCAGCAACGGAAAGACGGCTGATATCTTCAGTGCTGACAACAACGGAGACACCAGCATTGAAAAGACCGCCGATATCTCCAGTGCTGACAACACGGGATACCAGATCGCCGGTGCTGACAACAATGGAGACACCAGCAACGGGAAAGACTGCCAATATCTCCAGTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0000959 GO:0000963 GO:0001522 GO:0002153 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016556 GO:0016853 GO:0016866 GO:0031119 GO:0031974 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070525 GO:0070900 GO:0070902 GO:0071704 GO:0090304 GO:0090646 GO:0097159 GO:0140053 GO:1900864 GO:1901360 GO:1901363 GO:1990481

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_1

Protein Analysis

571

Amino Acids

62.59

Weight (kDa)

5.49

Isoelectric Point (pI)

32.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_1	PF01416	295 - 397	4.1e-12	tRNA pseudouridine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20370 AT1G76120 AT1G76120
fragaria_vesca	FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_4g09040 FvH4_5g07491
malus_domestica	MD15G1005300.v1.1 MD15G1005400.v1.1
prunus_persica	Prupe.1G357900_v2.0.a1
pyrus_communis	pycom08g00460 pycom15g00380 pycom15g00390
rosa_chinensis	RchiOBHm_Chr6g0303831
rosa_laevigata	RLG00000011040
rosa_multiflora	Rmu_co8307379.1_g000001 Rmu_sc0012878.1_g000001 Rmu_sc0022895.1_g000001
rosa_roxburghii	Rroxscaffold_7G00164370
rosa_rugosa	Rorug06G0325200
rosa_samantha	Rh6AG439500 Rh6BG463500 Rh6CG451800 Rh6DG438700
rosa_wichuraiana	Rw6G038050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1200
AasI	GACNNNNNNGTC	1 cut(s)	329
AccB1I	GGYRCC	2 cut(s)	209, 257
AccBSI	CCGCTC	1 cut(s)	309
AccI	GTMKAC	1 cut(s)	372
AccII	CGCG	1 cut(s)	444
AccIII	TCCGGA	2 cut(s)	1096, 1363
AclWI	GGATC	1 cut(s)	515
AcsI	RAATTY	3 cut(s)	420, 670, 1001
AcuI	CTGAAG	3 cut(s)	774, 951, 1558
AcyI	GRCGYC	5 cut(s)	177, 210, 258, 330, 452
AfaI	GTAC	5 cut(s)	319, 437, 491, 892, 1172
AfiI	CCNNNNNNNGG	5 cut(s)	609, 1163, 1210, 1370, 1686
AgsI	TTSAA	3 cut(s)	1001, 1270, 1607
AjnI	CCWGG	2 cut(s)	345, 885
AluBI	AGCT	4 cut(s)	76, 1032, 1118, 1265
AluI	AGCT	4 cut(s)	76, 1032, 1118, 1265
Alw21I	GWGCWC	2 cut(s)	602, 1096
Alw26I	GTCTC	6 cut(s)	1209, 1383, 1485, 1536, 1587, 1668
AlwI	GGATC	1 cut(s)	515
Aor13HI	TCCGGA	2 cut(s)	1096, 1363
AoxI	GGCC	4 cut(s)	303, 312, 343, 358
ApaI	GGGCCC	1 cut(s)	307
ApeKI	GCWGC	1 cut(s)	941
ApoI	RAATTY	3 cut(s)	420, 670, 1001
Asp700I	GAANNNNTTC	1 cut(s)	470
AspLEI	GCGC	5 cut(s)	212, 260, 338, 444, 642
AspS9I	GGNCC	7 cut(s)	274, 303, 304, 313, 343, 358, 391
AsuC2I	CCSGG	2 cut(s)	207, 381
AsuHPI	GGTGA	4 cut(s)	27, 671, 854, 904
AsuII	TTCGAA	2 cut(s)	404, 668
AvaII	GGWCC	2 cut(s)	274, 391
AxyI	CCTNAGG	1 cut(s)	1158
BaeGI	GKGCMC	2 cut(s)	307, 532
BanI	GGYRCC	2 cut(s)	209, 257
BanII	GRGCYC	2 cut(s)	307, 1332
BarI	GAAGNNNNNNTAC	2 cut(s)	1268, 1300
BbsI	GAAGAC	1 cut(s)	939
Bbv12I	GWGCWC	2 cut(s)	602, 1096
BbvI	GCAGC	1 cut(s)	928
BccI	CCATC	3 cut(s)	227, 625, 1050
BceAI	ACGGC	5 cut(s)	245, 323, 465, 1167, 1578
BcgI	CGANNNNNNTGC	2 cut(s)	1391, 1425
BciT130I	CCWGG	2 cut(s)	347, 887
BciVI	GTATCC	3 cut(s)	176, 1435, 1637
BcnI	CCSGG	2 cut(s)	207, 381
BcoDI	GTCTC	6 cut(s)	1209, 1383, 1485, 1536, 1587, 1668
BfaI	CTAG	3 cut(s)	525, 918, 1446
BfoI	RGCGCY	3 cut(s)	213, 261, 339
BfuI	GTATCC	3 cut(s)	176, 1435, 1637
BglI	GCCNNNNNGGC	1 cut(s)	311
BisI	GCNGC	6 cut(s)	50, 53, 117, 145, 175, 942
BlsI	GCNGC	6 cut(s)	51, 54, 118, 146, 176, 943
Bme1390I	CCNGG	4 cut(s)	207, 347, 381, 887
Bme18I	GGWCC	2 cut(s)	274, 391
BmgT120I	GGNCC	7 cut(s)	274, 303, 304, 313, 343, 358, 391
BmiI	GGNNCC	5 cut(s)	211, 259, 305, 1155, 1300
BmrFI	CCNGG	4 cut(s)	207, 347, 381, 887
BmrI	ACTGGG	1 cut(s)	310
BmsI	GCATC	2 cut(s)	144, 1407
BmuI	ACTGGG	1 cut(s)	310
BpiI	GAAGAC	1 cut(s)	939
BpmI	CTGGAG	4 cut(s)	1405, 1507, 1609, 1691
Bpu10I	CCTNAGC	1 cut(s)	288
Bpu14I	TTCGAA	2 cut(s)	404, 668
BpuEI	CTTGAG	1 cut(s)	895
BpuMI	CCSGG	2 cut(s)	207, 381
BsaHI	GRCGYC	5 cut(s)	177, 210, 258, 330, 452
BsaJI	CCNNGG	6 cut(s)	187, 205, 339, 380, 886, 978
BsaWI	WCCGGW	2 cut(s)	1096, 1363
BsaXI	ACNNNNNCTCC	2 cut(s)	1091, 1121
Bsc4I	CCNNNNNNNGG	5 cut(s)	609, 1163, 1210, 1370, 1686
Bse118I	RCCGGY	3 cut(s)	360, 1472, 1655
Bse1I	ACTGG	5 cut(s)	305, 1422, 1524, 1626, 1708
Bse21I	CCTNAGG	1 cut(s)	1158
Bse3DI	GCAATG	2 cut(s)	729, 1456
BseAI	TCCGGA	2 cut(s)	1096, 1363
BseBI	CCWGG	2 cut(s)	347, 887
BseDI	CCNNGG	6 cut(s)	187, 205, 339, 380, 886, 978
BseGI	GGATG	2 cut(s)	1135, 1183
BseLI	CCNNNNNNNGG	5 cut(s)	609, 1163, 1210, 1370, 1686
BseMI	GCAATG	2 cut(s)	729, 1456
BseMII	CTCAG	3 cut(s)	780, 1149, 1296
BseNI	ACTGG	5 cut(s)	305, 1422, 1524, 1626, 1708
BseRI	GAGGAG	2 cut(s)	499, 590
BseSI	GKGCMC	2 cut(s)	307, 532
BseXI	GCAGC	1 cut(s)	928
Bsh1236I	CGCG	1 cut(s)	444
BshFI	GGCC	4 cut(s)	305, 314, 345, 360
BshNI	GGYRCC	2 cut(s)	209, 257
BsiHKAI	GWGCWC	2 cut(s)	602, 1096
BsiSI	CCGG	8 cut(s)	207, 255, 361, 380, 1097, 1364, 1473, 1656
BslI	CCNNNNNNNGG	5 cut(s)	609, 1163, 1210, 1370, 1686
BsmAI	GTCTC	6 cut(s)	1209, 1383, 1485, 1536, 1587, 1668
BsmI	GAATGC	1 cut(s)	198
BsnI	GGCC	4 cut(s)	305, 314, 345, 360
Bsp119I	TTCGAA	2 cut(s)	404, 668
Bsp120I	GGGCCC	1 cut(s)	303
Bsp1286I	GDGCHC	5 cut(s)	307, 532, 602, 1096, 1332
Bsp13I	TCCGGA	2 cut(s)	1096, 1363
Bsp143I	GATC	4 cut(s)	32, 478, 520, 1650
BspANI	GGCC	4 cut(s)	305, 314, 345, 360
BspCNI	CTCAG	3 cut(s)	781, 1150, 1297
BspEI	TCCGGA	2 cut(s)	1096, 1363
BspFNI	CGCG	1 cut(s)	444
BspHI	TCATGA	2 cut(s)	1080, 1222
BspLI	GGNNCC	5 cut(s)	211, 259, 305, 1155, 1300
BspPI	GGATC	1 cut(s)	515
BspT104I	TTCGAA	2 cut(s)	404, 668
BspT107I	GGYRCC	2 cut(s)	209, 257
BsrBI	CCGCTC	1 cut(s)	309
BsrDI	GCAATG	2 cut(s)	729, 1456
BsrFI	RCCGGY	3 cut(s)	360, 1472, 1655
BsrI	ACTGG	5 cut(s)	305, 1422, 1524, 1626, 1708
BssAI	RCCGGY	3 cut(s)	360, 1472, 1655
BssECI	CCNNGG	6 cut(s)	187, 205, 339, 380, 886, 978
BssMI	GATC	4 cut(s)	32, 478, 520, 1650
BssNI	GRCGYC	5 cut(s)	177, 210, 258, 330, 452
BssT1I	CCWWGG	1 cut(s)	187
Bst2UI	CCWGG	2 cut(s)	347, 887
Bst4CI	ACNGT	3 cut(s)	982, 988, 1091
BstACI	GRCGYC	5 cut(s)	177, 210, 258, 330, 452
BstBI	TTCGAA	2 cut(s)	404, 668
BstC8I	GCNNGC	2 cut(s)	307, 1068
BstDEI	CTNAG	5 cut(s)	288, 789, 924, 1158, 1305
BstDSI	CCRYGG	2 cut(s)	339, 978
BstF5I	GGATG	2 cut(s)	1135, 1183
BstFNI	CGCG	1 cut(s)	444
BstH2I	RGCGCY	3 cut(s)	213, 261, 339
BstHHI	GCGC	5 cut(s)	212, 260, 338, 444, 642
BstKTI	GATC	4 cut(s)	35, 481, 523, 1653
BstMAI	GTCTC	6 cut(s)	1209, 1383, 1485, 1536, 1587, 1668
BstMBI	GATC	4 cut(s)	32, 478, 520, 1650
BstMWI	GCNNNNNNNGC	6 cut(s)	141, 311, 342, 597, 1336, 1469
BstNI	CCWGG	2 cut(s)	347, 887
BstSCI	CCNGG	4 cut(s)	205, 345, 379, 885
BstSLI	GKGCMC	2 cut(s)	307, 532
BstUI	CGCG	1 cut(s)	444
BstV1I	GCAGC	1 cut(s)	928
BstV2I	GAAGAC	1 cut(s)	939
Bsu36I	CCTNAGG	1 cut(s)	1158
BsuI	GTATCC	3 cut(s)	176, 1435, 1637
BsuRI	GGCC	4 cut(s)	305, 314, 345, 360
BtgI	CCRYGG	2 cut(s)	339, 978
BtsCI	GGATG	2 cut(s)	1135, 1183
BtsI	GCAGTG	2 cut(s)	966, 1077
BtsIMutI	CAGTG	8 cut(s)	966, 1077, 1302, 1429, 1531, 1582, 1633, 1715
Cac8I	GCNNGC	2 cut(s)	307, 1068
CciI	TCATGA	2 cut(s)	1080, 1222
CfoI	GCGC	5 cut(s)	212, 260, 338, 444, 642
Cfr10I	RCCGGY	3 cut(s)	360, 1472, 1655
Cfr13I	GGNCC	7 cut(s)	274, 303, 304, 313, 343, 358, 391
CseI	GACGC	3 cut(s)	166, 319, 441
Csp6I	GTAC	5 cut(s)	318, 436, 490, 891, 1171
CviAII	CATG	3 cut(s)	1036, 1081, 1223
CviQI	GTAC	5 cut(s)	318, 436, 490, 891, 1171
DdeI	CTNAG	5 cut(s)	288, 789, 924, 1158, 1305
DinI	GGCGCC	2 cut(s)	211, 259
DpnI	GATC	4 cut(s)	34, 480, 522, 1652
DpnII	GATC	4 cut(s)	32, 478, 520, 1650
DrdI	GACNNNNNNGTC	1 cut(s)	329
DseDI	GACNNNNNNGTC	1 cut(s)	329
EciI	GGCGGA	2 cut(s)	36, 628
Eco130I	CCWWGG	1 cut(s)	187
Eco24I	GRGCYC	2 cut(s)	307, 1332
Eco32I	GATATC	3 cut(s)	1417, 1570, 1621
Eco47I	GGWCC	2 cut(s)	274, 391
Eco57I	CTGAAG	3 cut(s)	774, 951, 1558
Eco81I	CCTNAGG	1 cut(s)	1158
EcoRI	GAATTC	1 cut(s)	670
EcoRII	CCWGG	2 cut(s)	345, 885
EcoRV	GATATC	3 cut(s)	1417, 1570, 1621
EcoT14I	CCWWGG	1 cut(s)	187
EcoT38I	GRGCYC	2 cut(s)	307, 1332
EgeI	GGCGCC	2 cut(s)	211, 259
EheI	GGCGCC	2 cut(s)	211, 259
ErhI	CCWWGG	1 cut(s)	187
FaeI	CATG	3 cut(s)	1039, 1084, 1226
FatI	CATG	3 cut(s)	1035, 1080, 1222
FauI	CCCGC	3 cut(s)	314, 384, 423
FauNDI	CATATG	1 cut(s)	717
FblI	GTMKAC	1 cut(s)	372
Fnu4HI	GCNGC	6 cut(s)	50, 53, 117, 145, 175, 942
FokI	GGATG	2 cut(s)	1142, 1190
FriOI	GRGCYC	2 cut(s)	307, 1332
Fsp4HI	GCNGC	6 cut(s)	50, 53, 117, 145, 175, 942
FspBI	CTAG	3 cut(s)	525, 918, 1446
GlaI	GCGC	5 cut(s)	211, 259, 337, 443, 641
GluI	GCNGC	6 cut(s)	50, 53, 117, 145, 175, 942
GsuI	CTGGAG	4 cut(s)	1405, 1507, 1609, 1691
HaeII	RGCGCY	3 cut(s)	213, 261, 339
HaeIII	GGCC	4 cut(s)	305, 314, 345, 360
HapII	CCGG	8 cut(s)	207, 255, 361, 380, 1097, 1364, 1473, 1656
HgaI	GACGC	3 cut(s)	166, 319, 441
HhaI	GCGC	5 cut(s)	212, 260, 338, 444, 642
Hin1I	GRCGYC	5 cut(s)	177, 210, 258, 330, 452
Hin1II	CATG	3 cut(s)	1039, 1084, 1226
Hin6I	GCGC	5 cut(s)	210, 258, 336, 442, 640
HinP1I	GCGC	5 cut(s)	210, 258, 336, 442, 640
HincII	GTYRAC	2 cut(s)	373, 1135
HindII	GTYRAC	2 cut(s)	373, 1135
HindIII	AAGCTT	1 cut(s)	1263
HinfI	GANTC	4 cut(s)	677, 710, 870, 1100
HpaI	GTTAAC	1 cut(s)	1135
HpaII	CCGG	8 cut(s)	207, 255, 361, 380, 1097, 1364, 1473, 1656
HphI	GGTGA	4 cut(s)	27, 671, 854, 904
Hpy166II	GTNNAC	5 cut(s)	87, 373, 391, 966, 1135
Hpy188I	TCNGA	9 cut(s)	182, 426, 483, 571, 604, 676, 754, 790, 1047
Hpy188III	TCNNGA	8 cut(s)	266, 518, 874, 1081, 1097, 1157, 1223, 1364
Hpy8I	GTNNAC	5 cut(s)	87, 373, 391, 966, 1135
Hpy99I	CGWCG	4 cut(s)	104, 107, 182, 457
HpyAV	CCTTC	7 cut(s)	218, 797, 805, 814, 841, 847, 1310
HpyCH4III	ACNGT	3 cut(s)	982, 988, 1091
HpyCH4IV	ACGT	2 cut(s)	492, 1137
HpyCH4V	TGCA	6 cut(s)	196, 944, 971, 1123, 1339, 1463
HpyF10VI	GCNNNNNNNGC	6 cut(s)	141, 311, 342, 597, 1336, 1469
HpyF3I	CTNAG	5 cut(s)	288, 789, 924, 1158, 1305
HpySE526I	ACGT	2 cut(s)	492, 1137
Hsp92I	GRCGYC	5 cut(s)	177, 210, 258, 330, 452
Hsp92II	CATG	3 cut(s)	1039, 1084, 1226
HspAI	GCGC	5 cut(s)	210, 258, 336, 442, 640
KasI	GGCGCC	2 cut(s)	209, 257
Kpn2I	TCCGGA	2 cut(s)	1096, 1363
KspAI	GTTAAC	1 cut(s)	1135
Kzo9I	GATC	4 cut(s)	32, 478, 520, 1650
LmnI	GCTCC	5 cut(s)	605, 1044, 1099, 1159, 1298
Lsp1109I	GCAGC	1 cut(s)	928
LweI	GCATC	2 cut(s)	144, 1407
MaeI	CTAG	3 cut(s)	525, 918, 1446
MaeII	ACGT	2 cut(s)	492, 1137
MaeIII	GTNAC	3 cut(s)	445, 881, 982
MalI	GATC	4 cut(s)	34, 480, 522, 1652
MbiI	CCGCTC	1 cut(s)	309
MboI	GATC	4 cut(s)	32, 478, 520, 1650
MboII	GAAGA	9 cut(s)	119, 151, 154, 248, 478, 944, 1180, 1238, 1564
MhlI	GDGCHC	5 cut(s)	307, 532, 602, 1096, 1332
MluCI	AATT	8 cut(s)	400, 420, 650, 670, 693, 1001, 1059, 1357
Mly113I	GGCGCC	2 cut(s)	210, 258
MlyI	GAGTC	1 cut(s)	1109
MmeI	TCCRAC	1 cut(s)	160
MroI	TCCGGA	2 cut(s)	1096, 1363
MroXI	GAANNNNTTC	1 cut(s)	470
MseI	TTAA	5 cut(s)	459, 552, 864, 1058, 1134
MspI	CCGG	8 cut(s)	207, 255, 361, 380, 1097, 1364, 1473, 1656
MspR9I	CCNGG	4 cut(s)	207, 347, 381, 887
Mva1269I	GAATGC	1 cut(s)	198
MvaI	CCWGG	2 cut(s)	347, 887
MvnI	CGCG	1 cut(s)	444
MwoI	GCNNNNNNNGC	6 cut(s)	141, 311, 342, 597, 1336, 1469
NarI	GGCGCC	2 cut(s)	210, 258
NciI	CCSGG	2 cut(s)	207, 381
NdeI	CATATG	1 cut(s)	717
NdeII	GATC	4 cut(s)	32, 478, 520, 1650
NlaIII	CATG	3 cut(s)	1039, 1084, 1226
NlaIV	GGNNCC	5 cut(s)	211, 259, 305, 1155, 1300
NmeAIII	GCCGAG	1 cut(s)	290
NmuCI	GTSAC	1 cut(s)	445
NspV	TTCGAA	2 cut(s)	404, 668
PagI	TCATGA	2 cut(s)	1080, 1222
PcsI	WCGNNNNNNNCGW	1 cut(s)	461
PctI	GAATGC	1 cut(s)	198
PdmI	GAANNNNTTC	1 cut(s)	470
PfeI	GAWTC	3 cut(s)	677, 710, 870
PflFI	GACNNNGTC	1 cut(s)	451
PkrI	GCNGC	6 cut(s)	51, 54, 118, 146, 176, 943
PleI	GAGTC	1 cut(s)	1108
PluTI	GGCGCC	2 cut(s)	213, 261
PpsI	GAGTC	1 cut(s)	1108
PsiI	TTATAA	1 cut(s)	1200
Psp6I	CCWGG	2 cut(s)	345, 885
PspGI	CCWGG	2 cut(s)	345, 885
PspN4I	GGNNCC	5 cut(s)	211, 259, 305, 1155, 1300
PspOMI	GGGCCC	1 cut(s)	303
PspPI	GGNCC	7 cut(s)	274, 303, 304, 313, 343, 358, 391
PsyI	GACNNNGTC	1 cut(s)	451
RsaI	GTAC	5 cut(s)	319, 437, 491, 892, 1172
RsaNI	GTAC	5 cut(s)	318, 436, 490, 891, 1171
SalI	GTCGAC	1 cut(s)	371
SaqAI	TTAA	5 cut(s)	459, 552, 864, 1058, 1134
SatI	GCNGC	6 cut(s)	50, 53, 117, 145, 175, 942
Sau3AI	GATC	4 cut(s)	32, 478, 520, 1650
Sau96I	GGNCC	7 cut(s)	274, 303, 304, 313, 343, 358, 391
SchI	GAGTC	1 cut(s)	1109
ScrFI	CCNGG	4 cut(s)	207, 347, 381, 887
SduI	GDGCHC	5 cut(s)	307, 532, 602, 1096, 1332
SfaNI	GCATC	2 cut(s)	144, 1407
SfiI	GGCCNNNNNGGCC	1 cut(s)	311
SfoI	GGCGCC	2 cut(s)	211, 259
SfuI	TTCGAA	2 cut(s)	404, 668
SgrAI	CRCCGGYG	2 cut(s)	1472, 1655
SinI	GGWCC	2 cut(s)	274, 391
SmlI	CTYRAG	1 cut(s)	874
SmoI	CTYRAG	1 cut(s)	874
Sse9I	AATT	8 cut(s)	400, 420, 650, 670, 693, 1001, 1059, 1357
SspDI	GGCGCC	2 cut(s)	209, 257
SspMI	CTAG	3 cut(s)	525, 918, 1446
StyD4I	CCNGG	4 cut(s)	205, 345, 379, 885
StyI	CCWWGG	1 cut(s)	187
TaaI	ACNGT	3 cut(s)	982, 988, 1091
TaiI	ACGT	2 cut(s)	495, 1140
TaqI	TCGA	7 cut(s)	222, 234, 372, 404, 668, 1235, 1401
TasI	AATT	8 cut(s)	400, 420, 650, 670, 693, 1001, 1059, 1357
TatI	WGTACW	1 cut(s)	1170
TauI	GCSGC	5 cut(s)	52, 55, 119, 147, 177
TfiI	GAWTC	3 cut(s)	677, 710, 870
Tru1I	TTAA	5 cut(s)	459, 552, 864, 1058, 1134
Tru9I	TTAA	5 cut(s)	459, 552, 864, 1058, 1134
TscAI	CASTG	8 cut(s)	973, 1077, 1309, 1429, 1531, 1582, 1633, 1715
TseFI	GTSAC	1 cut(s)	445
TseI	GCWGC	1 cut(s)	941
Tsp45I	GTSAC	1 cut(s)	445
TspDTI	ATGAA	8 cut(s)	663, 734, 797, 1024, 1239, 1253, 1263, 1289
TspGWI	ACGGA	3 cut(s)	1518, 1569, 1605
TspRI	CASTG	8 cut(s)	973, 1077, 1309, 1429, 1531, 1582, 1633, 1715
Tth111I	GACNNNGTC	1 cut(s)	451
VpaK11BI	GGWCC	2 cut(s)	274, 391
XapI	RAATTY	3 cut(s)	420, 670, 1001
XmiI	GTMKAC	1 cut(s)	372
XmnI	GAANNNNTTC	1 cut(s)	470
XspI	CTAG	3 cut(s)	525, 918, 1446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.