RLG00000013606

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014869
Physical Location & Seq
Forward (+)
33975894 .. 33977378
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013606

Sequence Viewer

Length: 654 bp
ATGTTAAGCGAGGGTGTGAAGGTGTGGAAGACAATTGTAGATCTGCAGACCCGGGAGGTTACCCCTTCGCAGCAGGACACAATCCTGGAGATGCCTTATCACTCACACATGGCGCGGGCGGGGGAGGCCTTTGTTCATCCTGTTCCCCCTTCTTCCAAAAGGGGTCCGGCTGGCTGGAACCATTTTGGTGGCCGCCCAAAGAAGACTTTAGACAGAGTTGTTGGCACCCGTAGAGGAGACCACCCCCAAAAGGAGCAGGCTATGGTGCTAGAGGAGTCTAGGGCTAGTGGGAGCTCCGTGGTTCAGCAAATTGCTCTGGATGTCCCTAGGAATGGGCAACGTCGGGATTGGCTGACGTCACCATCAACTATAGAGGGAGAACAAGGTGAAGGTGGTGATGGGCAAACTGTGAATCCGCCTAGTACTCGTTCGGTCAATGACGAATCCGCAAAGCATGCTGGCAACAAGCCACTACAAATGCCGATGGGGACCACTTCTGAGGCCTTACGTCTCCAACTCATTGACGCCAACACTTGCCCCGACGAGACTATGTACATGCCAAGCCCCCATGGCCATGGCCAGCAGTCAGATGAGACTAGTCGCTTTGTGGTGCAGTCGGGGCAGGCTGAAGAGGATGGTGCTACAAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

23.69

Weight (kDa)

6.07

Isoelectric Point (pI)

68.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 224
AccII CGCG 1 cut(s) 115
AciI CCGC 5 cut(s) 115, 119, 193, 416, 447
AcoI YGGCCR 3 cut(s) 190, 571, 577
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 2 cut(s) 356, 525
AfaI GTAC 2 cut(s) 424, 554
AfiI CCNNNNNNNGG 2 cut(s) 250, 332
AhlI ACTAGT 1 cut(s) 596
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 1 cut(s) 294
AluI AGCT 1 cut(s) 294
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 4 cut(s) 231, 515, 539, 587
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 5 cut(s) 126, 190, 501, 571, 577
ApeKI GCWGC 1 cut(s) 70
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 2 cut(s) 164, 489
AsuC2I CCSGG 2 cut(s) 52, 53
AsuHPI GGTGA 3 cut(s) 351, 398, 407
AvaI CYCGRG 1 cut(s) 51
AvaII GGWCC 2 cut(s) 164, 489
AvrII CCTAGG 1 cut(s) 326
BalI TGGCCA 2 cut(s) 573, 579
BanI GGYRCC 1 cut(s) 224
BanII GRGCYC 1 cut(s) 296
BbsI GAAGAC 2 cut(s) 35, 209
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 82
BccI CCATC 4 cut(s) 370, 392, 478, 629
BciT130I CCWGG 1 cut(s) 86
BcnI CCSGG 2 cut(s) 52, 53
BcoDI GTCTC 4 cut(s) 231, 515, 539, 587
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 6 cut(s) 269, 279, 285, 327, 420, 597
BfmI CTRYAG 2 cut(s) 44, 369
BglI GCCNNNNNGGC 1 cut(s) 570
BglII AGATCT 1 cut(s) 40
BisI GCNGC 2 cut(s) 71, 193
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 2 cut(s) 72, 194
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 3 cut(s) 52, 53, 86
Bme18I GGWCC 2 cut(s) 164, 489
BmeT110I CYCGRG 1 cut(s) 51
BmgT120I GGNCC 2 cut(s) 164, 489
BmiI GGNNCC 4 cut(s) 165, 179, 226, 490
BmrFI CCNGG 3 cut(s) 52, 53, 86
BmsI GCATC 1 cut(s) 81
BpiI GAAGAC 2 cut(s) 35, 209
BpmI CTGGAG 1 cut(s) 107
BpuMI CCSGG 2 cut(s) 52, 53
BsaHI GRCGYC 2 cut(s) 356, 525
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 5 cut(s) 51, 297, 326, 568, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 332
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 5 cut(s) 51, 297, 326, 568, 574
BseGI GGATG 3 cut(s) 136, 325, 640
BseLI CCNNNNNNNGG 2 cut(s) 250, 332
BseMII CTCAG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 249, 287
BseXI GCAGC 1 cut(s) 82
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 115
BshFI GGCC 5 cut(s) 128, 192, 503, 573, 579
BshNI GGYRCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 2 cut(s) 52, 167
BslFI GGGAC 2 cut(s) 308, 502
BslI CCNNNNNNNGG 2 cut(s) 250, 332
BsmAI GTCTC 4 cut(s) 231, 515, 539, 587
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 2 cut(s) 308, 502
BsnI GGCC 5 cut(s) 128, 192, 503, 573, 579
Bso31I GGTCTC 1 cut(s) 231
BsoBI CYCGRG 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 296
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 1 cut(s) 40
Bsp19I CCATGG 2 cut(s) 568, 574
BspACI CCGC 5 cut(s) 115, 119, 193, 416, 447
BspANI GGCC 5 cut(s) 128, 192, 503, 573, 579
BspCNI CTCAG 1 cut(s) 490
BspFNI CGCG 1 cut(s) 115
BspLI GGNNCC 4 cut(s) 165, 179, 226, 490
BspMAI CTGCAG 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 231
BsrGI TGTACA 1 cut(s) 552
BssECI CCNNGG 5 cut(s) 51, 297, 326, 568, 574
BssMI GATC 1 cut(s) 40
BssNI GRCGYC 2 cut(s) 356, 525
BssT1I CCWWGG 3 cut(s) 326, 568, 574
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 409
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 2 cut(s) 356, 525
BstAPI GCANNNNNTGC 1 cut(s) 455
BstAUI TGTACA 1 cut(s) 552
BstC8I GCNNGC 7 cut(s) 117, 172, 258, 456, 460, 581, 624
BstDEI CTNAG 1 cut(s) 498
BstDSI CCRYGG 3 cut(s) 297, 568, 574
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 3 cut(s) 136, 325, 640
BstFNI CGCG 1 cut(s) 115
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 4 cut(s) 231, 515, 539, 587
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 4 cut(s) 125, 455, 570, 619
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 2 cut(s) 458, 559
BstPI GGTNACC 1 cut(s) 58
BstSCI CCNGG 3 cut(s) 50, 51, 84
BstSFI CTRYAG 2 cut(s) 44, 369
BstUI CGCG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 82
BstV2I GAAGAC 2 cut(s) 35, 209
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 2 cut(s) 188, 575
BstYI RGATCY 1 cut(s) 40
BsuRI GGCC 5 cut(s) 128, 192, 503, 573, 579
BtgI CCRYGG 3 cut(s) 297, 568, 574
BtsCI GGATG 3 cut(s) 136, 325, 640
Cac8I GCNNGC 7 cut(s) 117, 172, 258, 456, 460, 581, 624
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 164, 489
Cfr9I CCCGGG 1 cut(s) 51
CseI GACGC 1 cut(s) 533
Csp6I GTAC 2 cut(s) 423, 553
CviAII CATG 5 cut(s) 109, 455, 556, 569, 575
CviQI GTAC 2 cut(s) 423, 553
DdeI CTNAG 1 cut(s) 498
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
EaeI YGGCCR 3 cut(s) 190, 571, 577
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
EciI GGCGGA 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 3 cut(s) 326, 568, 574
Eco147I AGGCCT 2 cut(s) 128, 503
Eco24I GRGCYC 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 164, 489
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 51
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 3 cut(s) 326, 568, 574
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 3 cut(s) 326, 568, 574
Esp3I CGTCTC 1 cut(s) 515
FaeI CATG 5 cut(s) 112, 458, 559, 572, 578
FaiI YATR 8 cut(s) 110, 263, 371, 456, 551, 557, 570, 576
FaqI GGGAC 2 cut(s) 308, 502
FatI CATG 5 cut(s) 108, 454, 555, 568, 574
FauI CCCGC 2 cut(s) 108, 112
Fnu4HI GCNGC 2 cut(s) 71, 193
FokI GGATG 3 cut(s) 123, 332, 647
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 71, 193
FspBI CTAG 6 cut(s) 269, 279, 285, 327, 420, 597
GlaI GCGC 1 cut(s) 114
GluI GCNGC 2 cut(s) 71, 193
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 5 cut(s) 128, 192, 503, 573, 579
HapII CCGG 2 cut(s) 52, 167
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 2 cut(s) 356, 525
Hin1II CATG 5 cut(s) 112, 458, 559, 572, 578
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HinfI GANTC 3 cut(s) 275, 412, 443
HpaII CCGG 2 cut(s) 52, 167
HphI GGTGA 3 cut(s) 351, 398, 407
Hpy188I TCNGA 2 cut(s) 499, 589
Hpy188III TCNNGA 2 cut(s) 317, 344
Hpy99I CGWCG 2 cut(s) 345, 545
HpyAV CCTTC 4 cut(s) 13, 75, 159, 383
HpyCH4III ACNGT 1 cut(s) 409
HpyCH4IV ACGT 3 cut(s) 340, 356, 508
HpyCH4V TGCA 2 cut(s) 46, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 455, 570, 619
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 3 cut(s) 340, 356, 508
Hsp92I GRCGYC 2 cut(s) 356, 525
Hsp92II CATG 5 cut(s) 112, 458, 559, 572, 578
HspAI GCGC 1 cut(s) 113
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 253, 291, 299
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 81
MaeI CTAG 6 cut(s) 269, 279, 285, 327, 420, 597
MaeII ACGT 3 cut(s) 340, 356, 508
MaeIII GTNAC 2 cut(s) 58, 357
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 4 cut(s) 40, 144, 214, 641
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 2 cut(s) 573, 579
MluCI AATT 2 cut(s) 33, 309
MluNI TGGCCA 2 cut(s) 573, 579
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 8 cut(s) 4, 49, 118, 227, 265, 367, 493, 625
Mox20I TGGCCA 2 cut(s) 573, 579
MscI TGGCCA 2 cut(s) 573, 579
MseI TTAA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 186, 573
Msp20I TGGCCA 2 cut(s) 573, 579
MspI CCGG 2 cut(s) 52, 167
MspR9I CCNGG 3 cut(s) 52, 53, 86
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 115
MwoI GCNNNNNNNGC 4 cut(s) 125, 455, 570, 619
NciI CCSGG 2 cut(s) 52, 53
NcoI CCATGG 2 cut(s) 568, 574
NdeII GATC 1 cut(s) 40
NlaIII CATG 5 cut(s) 112, 458, 559, 572, 578
NlaIV GGNNCC 4 cut(s) 165, 179, 226, 490
NmuCI GTSAC 1 cut(s) 357
NspI RCATGY 2 cut(s) 458, 559
PaeI GCATGC 1 cut(s) 458
PceI AGGCCT 2 cut(s) 128, 503
PfeI GAWTC 2 cut(s) 412, 443
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 2 cut(s) 72, 194
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Psp124BI GAGCTC 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 84
PspEI GGTNACC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 4 cut(s) 165, 179, 226, 490
PspPI GGNCC 2 cut(s) 164, 489
PstI CTGCAG 1 cut(s) 48
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 2 cut(s) 424, 554
RsaNI GTAC 2 cut(s) 423, 553
RseI CAYNNNNRTG 2 cut(s) 186, 573
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 5
SatI GCNGC 2 cut(s) 71, 193
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 2 cut(s) 164, 489
ScaI AGTACT 1 cut(s) 424
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 52, 53, 86
SduI GDGCHC 1 cut(s) 296
SetI ASST 8 cut(s) 24, 60, 296, 343, 359, 388, 394, 511
SfaNI GCATC 1 cut(s) 81
SfcI CTRYAG 2 cut(s) 44, 369
SinI GGWCC 2 cut(s) 164, 489
SmaI CCCGGG 1 cut(s) 53
SmiMI CAYNNNNRTG 2 cut(s) 186, 573
SpeI ACTAGT 1 cut(s) 596
SphI GCATGC 1 cut(s) 458
Sse9I AATT 2 cut(s) 33, 309
SseBI AGGCCT 2 cut(s) 128, 503
SsiI CCGC 5 cut(s) 115, 119, 193, 416, 447
SspMI CTAG 6 cut(s) 269, 279, 285, 327, 420, 597
SstI GAGCTC 1 cut(s) 296
StuI AGGCCT 2 cut(s) 128, 503
StyD4I CCNGG 3 cut(s) 50, 51, 84
StyI CCWWGG 3 cut(s) 326, 568, 574
TaaI ACNGT 1 cut(s) 409
TaiI ACGT 3 cut(s) 343, 359, 511
TaqII GACCGA 1 cut(s) 421
TasI AATT 2 cut(s) 33, 309
TatI WGTACW 2 cut(s) 422, 552
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 2 cut(s) 412, 443
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 286
TspMI CCCGGG 1 cut(s) 51
VpaK11BI GGWCC 2 cut(s) 164, 489
XceI RCATGY 2 cut(s) 458, 559
XmaI CCCGGG 1 cut(s) 51
XmaJI CCTAGG 1 cut(s) 326
XspI CTAG 6 cut(s) 269, 279, 285, 327, 420, 597
ZraI GACGTC 1 cut(s) 357
ZrmI AGTACT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.