RLG00000022557

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014869
Physical Location & Seq
Forward (+)
3729927 .. 3731219
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022557

Sequence Viewer

Length: 420 bp
ATGCGTCATCACTCCCACTTGGCGCAGGTGGGGGAGGCTCCGGTTCGTCCTGTTGCACCAGCCTCAGACAGGGATCCTGTTGGCGGGAACCTTTTTAGTGGCCGATATGAGAAGCCTTCAGATACTGTTTTTGGCCTAGGAAGCCAACTCCAAAAGGAGCAAGGTGTGGTGATAGAGGATTCCGGTGGGAGCTCCGTGTCCCAAGAGTTGAATGACTTGCTTACGGAGGCAGGCAAAGAGGGAGTTGCAAAGAACTTGCGCTGGCTGGAGTCTCAGGGGTTTGTGGGACGTGTTTGCAACGAGCCGCTGCAAACCTTGATGGGGACCGCTTCTGAGGCCTCGCATCTCCAGTTTATTGCCACTAATACTTGCCCCGATGGGACTATGTACATGCCAAGCCCTCATGGTCATGGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

14.83

Weight (kDa)

5.4

Isoelectric Point (pI)

57.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 16
AciI CCGC 3 cut(s) 84, 305, 327
AclWI GGATC 2 cut(s) 68, 81
AcoI YGGCCR 2 cut(s) 100, 412
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 3 cut(s) 69, 83, 321
AflIII ACRYGT 1 cut(s) 289
AgsI TTSAA 1 cut(s) 211
AjiI CACGTC 1 cut(s) 290
AluBI AGCT 1 cut(s) 192
AluI AGCT 1 cut(s) 192
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 2 cut(s) 68, 81
AlwNI CAGNNNCTG 1 cut(s) 125
AoxI GGCC 4 cut(s) 100, 133, 336, 412
ApeKI GCWGC 1 cut(s) 307
AspA2I CCTAGG 1 cut(s) 136
AspLEI GCGC 2 cut(s) 25, 261
AspS9I GGNCC 1 cut(s) 324
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 324
AvrII CCTAGG 1 cut(s) 136
BalI TGGCCA 1 cut(s) 414
BamHI GGATCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 313, 371
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 16
BisI GCNGC 2 cut(s) 305, 308
BlnI CCTAGG 1 cut(s) 136
BlsI GCNGC 2 cut(s) 306, 309
Bme18I GGWCC 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 324
BmiI GGNNCC 4 cut(s) 39, 75, 89, 325
BmsI GCATC 1 cut(s) 352
BpmI CTGGAG 2 cut(s) 287, 332
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 2 cut(s) 40, 182
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 83, 321
Bse1I ACTGG 2 cut(s) 349, 415
BseDI CCNNGG 1 cut(s) 136
BseLI CCNNNNNNNGG 3 cut(s) 69, 83, 321
BseMII CTCAG 3 cut(s) 78, 287, 324
BseNI ACTGG 2 cut(s) 349, 415
BseXI GCAGC 1 cut(s) 294
BshFI GGCC 4 cut(s) 102, 135, 338, 414
BsiHKAI GWGCWC 1 cut(s) 194
BsiSI CCGG 2 cut(s) 41, 183
BslFI GGGAC 4 cut(s) 184, 300, 337, 394
BslI CCNNNNNNNGG 3 cut(s) 69, 83, 321
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 4 cut(s) 184, 300, 337, 394
BsnI GGCC 4 cut(s) 102, 135, 338, 414
Bsp1286I GDGCHC 1 cut(s) 194
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 3 cut(s) 84, 305, 327
BspANI GGCC 4 cut(s) 102, 135, 338, 414
BspCNI CTCAG 3 cut(s) 77, 286, 325
BspLI GGNNCC 4 cut(s) 39, 75, 89, 325
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 2 cut(s) 68, 81
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 2 cut(s) 349, 415
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 127
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 232, 263
BstDEI CTNAG 3 cut(s) 64, 273, 333
BstENI CCTNNNNNAGG 1 cut(s) 67
BstHHI GCGC 2 cut(s) 25, 261
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 141, 335
BstNSI RCATGY 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 4 cut(s) 102, 135, 338, 414
BtrI CACGTC 1 cut(s) 290
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 232, 263
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 2 cut(s) 25, 261
Cfr13I GGNCC 1 cut(s) 324
Csp6I GTAC 1 cut(s) 388
CviAII CATG 3 cut(s) 391, 404, 410
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 64, 273, 333
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 2 cut(s) 100, 412
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 3 cut(s) 394, 407, 413
FaiI YATR 5 cut(s) 108, 386, 392, 405, 411
FaqI GGGAC 4 cut(s) 184, 300, 337, 394
FatI CATG 3 cut(s) 390, 403, 409
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 2 cut(s) 305, 308
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 305, 308
FspBI CTAG 1 cut(s) 137
GlaI GCGC 2 cut(s) 24, 260
GluI GCNGC 2 cut(s) 305, 308
GsuI CTGGAG 2 cut(s) 287, 332
HaeIII GGCC 4 cut(s) 102, 135, 338, 414
HapII CCGG 2 cut(s) 41, 183
HhaI GCGC 2 cut(s) 25, 261
Hin1II CATG 3 cut(s) 394, 407, 413
Hin6I GCGC 2 cut(s) 23, 259
HinP1I GCGC 2 cut(s) 23, 259
HinfI GANTC 2 cut(s) 179, 269
HpaII CCGG 2 cut(s) 41, 183
HphI GGTGA 1 cut(s) 181
Hpy188I TCNGA 3 cut(s) 67, 121, 334
HpyAV CCTTC 1 cut(s) 126
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 4 cut(s) 56, 248, 297, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 335
HpyF3I CTNAG 3 cut(s) 64, 273, 333
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 3 cut(s) 394, 407, 413
HspAI GCGC 2 cut(s) 23, 259
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 4 cut(s) 43, 157, 189, 197
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 1 cut(s) 352
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 289
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 194
MlsI TGGCCA 1 cut(s) 414
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 8 cut(s) 28, 73, 169, 220, 232, 328, 349, 411
Mox20I TGGCCA 1 cut(s) 414
MscI TGGCCA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 2 cut(s) 41, 183
MwoI GCNNNNNNNGC 2 cut(s) 141, 335
NdeII GATC 1 cut(s) 73
NlaIII CATG 3 cut(s) 394, 407, 413
NlaIV GGNNCC 4 cut(s) 39, 75, 89, 325
NspI RCATGY 1 cut(s) 394
PaqCI CACCTGC 1 cut(s) 16
PceI AGGCCT 1 cut(s) 338
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 2 cut(s) 306, 309
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 194
PspN4I GGNNCC 4 cut(s) 39, 75, 89, 325
PspPI GGNCC 1 cut(s) 324
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 408
SacI GAGCTC 1 cut(s) 194
SatI GCNGC 2 cut(s) 305, 308
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 1 cut(s) 324
SchI GAGTC 1 cut(s) 278
SduI GDGCHC 1 cut(s) 194
SetI ASST 6 cut(s) 30, 93, 166, 194, 292, 317
SfaNI GCATC 1 cut(s) 352
SinI GGWCC 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 408
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 3 cut(s) 84, 305, 327
SspMI CTAG 1 cut(s) 137
SstI GAGCTC 1 cut(s) 194
StuI AGGCCT 1 cut(s) 338
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 292
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 1 cut(s) 179
TseI GCWGC 1 cut(s) 307
TspGWI ACGGA 2 cut(s) 184, 239
VpaK11BI GGWCC 1 cut(s) 324
XagI CCTNNNNNAGG 1 cut(s) 67
XceI RCATGY 1 cut(s) 394
XmaJI CCTAGG 1 cut(s) 136
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.