RLG00000013738
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015736
Physical Location & Seq
Forward (+)
37661854 .. 37665100
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013738

Sequence Viewer

Length: 807 bp
ATGGCGAAACAACCAGCTCGCGTCCTCGTTACCAGAGCCACAGGTGAAATCGGGTATGCTCTTGTACCTATGATCGCAAAAGGAGTGATGCTCGGAGCTGACCAGCCCATGATCCTTCACCTTCTTGATGAACCATTCGCTGCTGAAGCATTGAATGGTGTCAAGATGATACTGCGATCGTCGACTGATTTCATACTTGGATTGTTCATTGTTGCAGGTGTTGTTGCTACATCTGATGTTGTGAAGGCATGCACTGGAGTCAACATTGCAATCATAGTTGGTGGCTTCCCAGGGAAAGAGGGCATGGTAAAGAAAGATGTCCTGTCAGAGAAAGATGCTATCTCCATTCGCGGAGCCGCAGTTCACGAGAGTAAAAACGTCAAGCTCTTAGACCCACTTATAAGTTCTCCATCAAAAGCCGTCTGCATTTCTCCATATCAGAGACTGTCTGCTGATGTTCCTGTACAACTTCATGAGAGGTTTCAAGGTGAGAGTATCACTAGGTCCTCATTTATAGAGGAACCTGGAGACCATGGAGAAGCACACTTAGTAGCTCTGGGTGAATTAGAATTCCGTAAACAATTTCTAATATTAAGTGATGCTGGAGGAATGAAGTTGAAAGATCTGATGGCGGCAGAGGACATTAGGCATTGGAAATATTTGCCAATGCCTGATGCCCCTTTCATGTTTTTGATGTATTATAGAGTTTTGATTGGGATTCTGGAAGCACACAGTGTCTTGGGTTGTTACGTCAACGTGGATGGGAGTTACAGATTCAAGGATTACTCGCAGCAACCCCCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

269

Amino Acids

29.23

Weight (kDa)

6.17

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 7 - 114 9.1e-06 lactate/malate dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 401
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 182
AccII CGCG 2 cut(s) 21, 351
AciI CCGC 3 cut(s) 351, 357, 632
AclWI GGATC 1 cut(s) 106
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 2 cut(s) 66, 465
AgsI TTSAA 4 cut(s) 154, 485, 619, 778
AjnI CCWGG 2 cut(s) 289, 523
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 4 cut(s) 17, 98, 385, 554
AluI AGCT 4 cut(s) 17, 98, 385, 554
Alw26I GTCTC 2 cut(s) 436, 522
AlwI GGATC 1 cut(s) 106
AlwNI CAGNNNCTG 1 cut(s) 445
ApeKI GCWGC 2 cut(s) 140, 790
ApoI RAATTY 1 cut(s) 569
AspS9I GGNCC 1 cut(s) 504
AsuHPI GGTGA 4 cut(s) 56, 110, 500, 572
AvaII GGWCC 1 cut(s) 504
BauI CACGAG 1 cut(s) 365
BbvI GCAGC 2 cut(s) 127, 802
BccI CCATC 3 cut(s) 418, 622, 755
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 291, 525
BcoDI GTCTC 2 cut(s) 436, 522
BfaI CTAG 1 cut(s) 501
BfuAI ACCTGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 622
BisI GCNGC 4 cut(s) 141, 357, 633, 791
BlsI GCNGC 4 cut(s) 142, 358, 634, 792
Bme1390I CCNGG 2 cut(s) 291, 525
Bme18I GGWCC 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 504
BmiI GGNNCC 2 cut(s) 355, 522
BmrFI CCNGG 2 cut(s) 291, 525
BmsI GCATC 4 cut(s) 78, 325, 589, 664
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 3 cut(s) 276, 546, 624
BsaI GGTCTC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 289, 290, 532
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 264
BseBI CCWGG 2 cut(s) 291, 525
BseDI CCNNGG 3 cut(s) 289, 290, 532
BseGI GGATG 1 cut(s) 766
BseMI GCAATG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 2 cut(s) 127, 802
Bsh1236I CGCG 2 cut(s) 21, 351
Bsh1285I CGRYCG 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 179
BsmAI GTCTC 2 cut(s) 436, 522
Bso31I GGTCTC 1 cut(s) 522
Bsp1407I TGTACA 1 cut(s) 463
Bsp143I GATC 4 cut(s) 72, 111, 176, 622
Bsp19I CCATGG 1 cut(s) 532
BspACI CCGC 3 cut(s) 351, 357, 632
BspFNI CGCG 2 cut(s) 21, 351
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 2 cut(s) 355, 522
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 106
BspTNI GGTCTC 1 cut(s) 522
BsrDI GCAATG 1 cut(s) 264
BsrGI TGTACA 1 cut(s) 463
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 3 cut(s) 289, 290, 532
BssMI GATC 4 cut(s) 72, 111, 176, 622
BssSI CACGAG 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 532
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 2 cut(s) 291, 525
Bst4CI ACNGT 2 cut(s) 447, 734
BstAUI TGTACA 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 19, 250
BstDEI CTNAG 2 cut(s) 388, 547
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 1 cut(s) 766
BstFNI CGCG 2 cut(s) 21, 351
BstKTI GATC 4 cut(s) 75, 114, 179, 625
BstMAI GTCTC 2 cut(s) 436, 522
BstMBI GATC 4 cut(s) 72, 111, 176, 622
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 2 cut(s) 291, 525
BstNSI RCATGY 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 289, 523
BstUI CGCG 2 cut(s) 21, 351
BstV1I GCAGC 2 cut(s) 127, 802
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 1 cut(s) 766
BtsIMutI CAGTG 2 cut(s) 252, 739
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 19, 250
CaiI CAGNNNCTG 1 cut(s) 445
CciI TCATGA 1 cut(s) 472
Cfr13I GGNCC 1 cut(s) 504
CseI GACGC 1 cut(s) 10
Csp6I GTAC 2 cut(s) 65, 464
CviAII CATG 6 cut(s) 109, 249, 304, 473, 533, 685
CviJI RGCY 9 cut(s) 17, 38, 98, 106, 285, 356, 385, 419, 554
CviKI_1 RGCY 9 cut(s) 17, 38, 98, 106, 285, 356, 385, 419, 554
CviQI GTAC 2 cut(s) 65, 464
DdeI CTNAG 2 cut(s) 388, 547
DpnI GATC 4 cut(s) 74, 113, 178, 624
DpnII GATC 4 cut(s) 72, 111, 176, 622
DraIII CACNNNGTG 1 cut(s) 734
Eco130I CCWWGG 1 cut(s) 532
Eco31I GGTCTC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 504
EcoRI GAATTC 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 289, 523
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 6 cut(s) 112, 252, 307, 476, 536, 688
FatI CATG 6 cut(s) 108, 248, 303, 472, 532, 684
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 4 cut(s) 141, 357, 633, 791
FokI GGATG 1 cut(s) 773
Fsp4HI GCNGC 4 cut(s) 141, 357, 633, 791
FspBI CTAG 1 cut(s) 501
GluI GCNGC 4 cut(s) 141, 357, 633, 791
GsuI CTGGAG 3 cut(s) 276, 546, 624
HgaI GACGC 1 cut(s) 10
Hin1II CATG 6 cut(s) 112, 252, 307, 476, 536, 688
HincII GTYRAC 3 cut(s) 183, 262, 754
HindII GTYRAC 3 cut(s) 183, 262, 754
HinfI GANTC 3 cut(s) 258, 718, 774
HphI GGTGA 4 cut(s) 56, 110, 500, 572
Hpy166II GTNNAC 5 cut(s) 183, 262, 364, 578, 754
Hpy188I TCNGA 5 cut(s) 95, 235, 328, 441, 627
Hpy188III TCNNGA 5 cut(s) 125, 163, 365, 473, 722
Hpy8I GTNNAC 5 cut(s) 183, 262, 364, 578, 754
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 3 cut(s) 125, 131, 238
HpyCH4III ACNGT 2 cut(s) 447, 734
HpyCH4IV ACGT 3 cut(s) 378, 750, 756
HpyCH4V TGCA 4 cut(s) 215, 252, 269, 426
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 2 cut(s) 388, 547
HpySE526I ACGT 3 cut(s) 378, 750, 756
Hsp92II CATG 6 cut(s) 112, 252, 307, 476, 536, 688
Kzo9I GATC 4 cut(s) 72, 111, 176, 622
LmnI GCTCC 2 cut(s) 95, 353
Lsp1109I GCAGC 2 cut(s) 127, 802
LweI GCATC 4 cut(s) 78, 325, 589, 664
MaeI CTAG 1 cut(s) 501
MaeII ACGT 3 cut(s) 378, 750, 756
MaeIII GTNAC 3 cut(s) 28, 746, 767
MalI GATC 4 cut(s) 74, 113, 178, 624
MboI GATC 4 cut(s) 72, 111, 176, 622
MflI RGATCY 1 cut(s) 622
MluCI AATT 3 cut(s) 563, 569, 581
MlyI GAGTC 1 cut(s) 267
MnlI CCTC 7 cut(s) 35, 292, 471, 511, 517, 599, 631
MseI TTAA 1 cut(s) 593
MspR9I CCNGG 2 cut(s) 291, 525
MvaI CCWGG 2 cut(s) 291, 525
MvnI CGCG 2 cut(s) 21, 351
MwoI GCNNNNNNNGC 1 cut(s) 146
NcoI CCATGG 1 cut(s) 532
NdeII GATC 4 cut(s) 72, 111, 176, 622
NlaIII CATG 6 cut(s) 112, 252, 307, 476, 536, 688
NlaIV GGNNCC 2 cut(s) 355, 522
NspI RCATGY 1 cut(s) 252
PaeI GCATGC 1 cut(s) 252
PagI TCATGA 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 206
PasI CCCWGGG 1 cut(s) 290
PfeI GAWTC 2 cut(s) 718, 774
PkrI GCNGC 4 cut(s) 142, 358, 634, 792
Ple19I CGATCG 1 cut(s) 179
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 504
PsiI TTATAA 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 289, 523
PspGI CCWGG 2 cut(s) 289, 523
PspN4I GGNNCC 2 cut(s) 355, 522
PspPI GGNCC 1 cut(s) 504
PspPPI RGGWCCY 1 cut(s) 504
PstNI CAGNNNCTG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 622
PvuI CGATCG 1 cut(s) 179
RsaI GTAC 2 cut(s) 66, 465
RsaNI GTAC 2 cut(s) 65, 464
SalI GTCGAC 1 cut(s) 181
SaqAI TTAA 1 cut(s) 593
SatI GCNGC 4 cut(s) 141, 357, 633, 791
Sau3AI GATC 4 cut(s) 72, 111, 176, 622
Sau96I GGNCC 1 cut(s) 504
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 2 cut(s) 291, 525
SfaNI GCATC 4 cut(s) 78, 325, 589, 664
SinI GGWCC 1 cut(s) 504
SphI GCATGC 1 cut(s) 252
Sse9I AATT 3 cut(s) 563, 569, 581
SsiI CCGC 3 cut(s) 351, 357, 632
SspI AATATT 2 cut(s) 591, 659
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 2 cut(s) 289, 523
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 2 cut(s) 447, 734
TaiI ACGT 3 cut(s) 381, 753, 759
TaqI TCGA 1 cut(s) 182
TasI AATT 3 cut(s) 563, 569, 581
TatI WGTACW 1 cut(s) 463
TauI GCSGC 2 cut(s) 359, 635
TfiI GAWTC 2 cut(s) 718, 774
Tru1I TTAA 1 cut(s) 593
Tru9I TTAA 1 cut(s) 593
TscAI CASTG 2 cut(s) 259, 739
TseI GCWGC 2 cut(s) 140, 790
TspDTI ATGAA 6 cut(s) 144, 181, 196, 461, 626, 673
TspGWI ACGGA 1 cut(s) 563
TspRI CASTG 2 cut(s) 259, 739
VpaK11BI GGWCC 1 cut(s) 504
XapI RAATTY 1 cut(s) 569
XceI RCATGY 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 182
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.