RLG00000019318
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015736
Physical Location & Seq
Forward (+)
50220826 .. 50222171
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019318

Sequence Viewer

Length: 435 bp
ATGTTCCTGTACAACTTCATGAGAGGTTTCAAGTGTGAGTGTGTTGCTGCTGCAGGTGAGAGTATCACTAGGTCCTCAGTTATAGAGGAACCTGGAGACCATGGAGAAGCACACTTAGTAGCTCTGGGTGAATTAGAATTCCGTAAACAATTTCTAATATTAAGTGATGCTGGAGGAATGAAGTTGAAAGATCTGATAGCAGCAGAGGACATTAGGCATTGGAAATATTTGGCAATGGTAACGTTTGAGATCAAAATTTGGGAATATCTAGGTCGAAAATGGATCAGTCCTGAAGACTGTCGATTGAGTTTTGATTGGGATTCTGGAAGCACACAGTGTCTTGGGTTGTTACGTCTACGTGGATGGGAGTTACAGATTCAAGGATTACTCGCAGCAGCCCCCTATTTAGGAACTAATTGGAAGGGATCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

145

Amino Acids

16.32

Weight (kDa)

5.62

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_helical PF26252 38 - 103 3.4e-06 RNA dependent RNA polymerase, helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 355
AclI AACGTT 1 cut(s) 242
AclWI GGATC 2 cut(s) 290, 433
AcsI RAATTY 2 cut(s) 137, 255
AcuI CTGAAG 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 3 cut(s) 31, 187, 380
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 290, 433
ApeKI GCWGC 5 cut(s) 47, 50, 200, 392, 395
ApoI RAATTY 2 cut(s) 137, 255
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 68, 140
AvaII GGWCC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 300
BbvI GCAGC 5 cut(s) 34, 37, 212, 404, 407
BccI CCATC 1 cut(s) 357
BciT130I CCWGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 2 cut(s) 69, 269
BfmI CTRYAG 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 190
BisI GCNGC 5 cut(s) 48, 51, 201, 393, 396
BlsI GCNGC 5 cut(s) 49, 52, 202, 394, 397
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 2 cut(s) 114, 192
BsaAI YACGTR 1 cut(s) 359
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 368
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 90
BseXI GCAGC 5 cut(s) 34, 37, 212, 404, 407
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 90
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 4 cut(s) 190, 249, 282, 425
Bsp19I CCATGG 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 89
BspHI TCATGA 2 cut(s) 18, 431
BspLI GGNNCC 1 cut(s) 90
BspMAI CTGCAG 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 2 cut(s) 290, 433
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 4 cut(s) 190, 249, 282, 425
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 299, 336
BstAUI TGTACA 1 cut(s) 9
BstBAI YACGTR 1 cut(s) 359
BstDEI CTNAG 2 cut(s) 76, 115
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 1 cut(s) 368
BstKTI GATC 4 cut(s) 193, 252, 285, 428
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 4 cut(s) 190, 249, 282, 425
BstNI CCWGG 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 5 cut(s) 34, 37, 212, 404, 407
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 2 cut(s) 190, 425
BstYI RGATCY 2 cut(s) 190, 425
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 341
BveI ACCTGC 1 cut(s) 44
CciI TCATGA 2 cut(s) 18, 431
Cfr13I GGNCC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 10
CviAII CATG 3 cut(s) 19, 101, 432
CviJI RGCY 2 cut(s) 122, 398
CviKI_1 RGCY 2 cut(s) 122, 398
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 76, 115
DpnI GATC 4 cut(s) 192, 251, 284, 427
DpnII GATC 4 cut(s) 190, 249, 282, 425
DraIII CACNNNGTG 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 100
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 3 cut(s) 22, 104, 435
FaiI YATR 4 cut(s) 20, 83, 102, 433
FatI CATG 3 cut(s) 18, 100, 431
FblI GTMKAC 1 cut(s) 355
Fnu4HI GCNGC 5 cut(s) 48, 51, 201, 393, 396
FokI GGATG 1 cut(s) 375
Fsp4HI GCNGC 5 cut(s) 48, 51, 201, 393, 396
FspBI CTAG 2 cut(s) 69, 269
GluI GCNGC 5 cut(s) 48, 51, 201, 393, 396
GsuI CTGGAG 2 cut(s) 114, 192
Hin1II CATG 3 cut(s) 22, 104, 435
HinfI GANTC 2 cut(s) 320, 376
HphI GGTGA 2 cut(s) 68, 140
Hpy166II GTNNAC 2 cut(s) 146, 356
Hpy188I TCNGA 1 cut(s) 195
Hpy188III TCNNGA 4 cut(s) 19, 290, 324, 432
Hpy8I GTNNAC 2 cut(s) 146, 356
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 2 cut(s) 299, 336
HpyCH4IV ACGT 3 cut(s) 242, 352, 358
HpyCH4V TGCA 1 cut(s) 53
HpyF3I CTNAG 2 cut(s) 76, 115
HpySE526I ACGT 3 cut(s) 242, 352, 358
Hsp92II CATG 3 cut(s) 22, 104, 435
Kzo9I GATC 4 cut(s) 190, 249, 282, 425
LpnPI CCDG 8 cut(s) 20, 39, 78, 105, 110, 156, 303, 309
Lsp1109I GCAGC 5 cut(s) 34, 37, 212, 404, 407
LweI GCATC 1 cut(s) 157
MaeI CTAG 2 cut(s) 69, 269
MaeII ACGT 3 cut(s) 242, 352, 358
MaeIII GTNAC 3 cut(s) 238, 348, 369
MalI GATC 4 cut(s) 192, 251, 284, 427
MboI GATC 4 cut(s) 190, 249, 282, 425
MboII GAAGA 2 cut(s) 305, 420
MflI RGATCY 2 cut(s) 190, 425
MluCI AATT 5 cut(s) 131, 137, 149, 255, 415
MnlI CCTC 5 cut(s) 17, 79, 85, 167, 199
MseI TTAA 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 100
NdeII GATC 4 cut(s) 190, 249, 282, 425
NlaIII CATG 3 cut(s) 22, 104, 435
NlaIV GGNNCC 1 cut(s) 90
PagI TCATGA 2 cut(s) 18, 431
PaqCI CACCTGC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 320, 376
PkrI GCNGC 5 cut(s) 49, 52, 202, 394, 397
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 72
Psp1406I AACGTT 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 72
PspPPI RGGWCCY 1 cut(s) 72
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 2 cut(s) 190, 425
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 5 cut(s) 48, 51, 201, 393, 396
Sau3AI GATC 4 cut(s) 190, 249, 282, 425
Sau96I GGNCC 1 cut(s) 72
ScrFI CCNGG 1 cut(s) 93
SetI ASST 9 cut(s) 28, 58, 74, 94, 124, 245, 274, 355, 361
SfaNI GCATC 1 cut(s) 157
SfcI CTRYAG 1 cut(s) 51
SinI GGWCC 1 cut(s) 72
Sse9I AATT 5 cut(s) 131, 137, 149, 255, 415
SspI AATATT 2 cut(s) 159, 227
SspMI CTAG 2 cut(s) 69, 269
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 2 cut(s) 299, 336
TaiI ACGT 3 cut(s) 245, 355, 361
TaqI TCGA 2 cut(s) 274, 301
TasI AATT 5 cut(s) 131, 137, 149, 255, 415
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 2 cut(s) 320, 376
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 341
TseI GCWGC 5 cut(s) 47, 50, 200, 392, 395
TspDTI ATGAA 3 cut(s) 7, 194, 420
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 137, 255
XmiI GTMKAC 1 cut(s) 355
XspI CTAG 2 cut(s) 69, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.