Rh6CG133900
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015736
Physical Location & Seq
Reverse (-)
16609830 .. 16620471
10642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG133900.1

Sequence Viewer

Length: 816 bp
ATGGCCTTATTCAAGAAGGCTCAGGTATCCCATCACAGGTCATGGACTAAACTTGCAGTTATGACAGCTTCTCAGCGCAATATAAGAGAGAGAAGAGAGTTCGTTTTTCAAAGCAAGGGAGGAAGCAGAATTTCTTACACATTGAATCTGGGTAAACACGGTATCGAGAAACAACCGTTTGAGCTCCCTGATTTCATAGCTGCAACTGGTACTGAGAAAATCAGACAGGACGATTCATATGTGGACAGCTACGTCAGCACCATTGGAGTTGATTTTAAAATCAGGACTGTGGAGCTAGATGGGAAGACAGTCAAGCTGCAGATTAGACTGTCTGCTGATGTTCCTGTACAACTTCATGAGAGGTTTCAAGGTGAGAGTATCACTAGGTCCTCAGTTATAGAGGAACCTGGAGACCATGGAGAAGCACACTTAGTAGCTCTGGGTGAATTAGAATTCCGTAAACAATTTCTAATATTAAGTGATACTGGAGGAATGAAGTTGAAAGATCTGATAGCGGCAGAGGACATTAGGCATTGGAAATATTTGCCAATGAGTTTTGATTGGGATTCTAGAAGCACACAGTGTCTTGGGTTGTTATGTCAACGTGGATGGGAGTTACAGATTCAAGATGAGGAGACAAATGGCTACTCGGCACAATATTCCTCAACTGGTTTTACTGATATGGGGAACACAGCTGATGGAGGAGTTCTTTCGTCAAGCCAGGAAACTCTAAGGAAAGCAGAGGAGATCTTTGGGACAGATAACAAAGATCTGTATTTATCATTTCAAGGATTGCTTAGCCGTAACATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

271

Amino Acids

30.66

Weight (kDa)

5.93

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 239 - 269 5.4e-09 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AciI CCGC 1 cut(s) 515
AcsI RAATTY 2 cut(s) 129, 452
AdeI CACNNNGTG 1 cut(s) 582
AfaI GTAC 2 cut(s) 211, 348
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 7 cut(s) 13, 110, 145, 368, 502, 626, 788
AjnI CCWGG 2 cut(s) 406, 720
AluBI AGCT 8 cut(s) 68, 184, 200, 249, 295, 316, 437, 695
AluI AGCT 8 cut(s) 68, 184, 200, 249, 295, 316, 437, 695
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 2 cut(s) 405, 629
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 200, 316
ApoI RAATTY 2 cut(s) 129, 452
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 1 cut(s) 387
AsuHPI GGTGA 2 cut(s) 383, 455
AvaII GGWCC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 187, 303
BccI CCATC 4 cut(s) 39, 293, 603, 692
BceAI ACGGC 1 cut(s) 786
BciT130I CCWGG 2 cut(s) 408, 722
BciVI GTATCC 1 cut(s) 37
BcoDI GTCTC 2 cut(s) 405, 629
BfaI CTAG 4 cut(s) 296, 384, 570, 814
BfmI CTRYAG 1 cut(s) 317
BfuI GTATCC 1 cut(s) 37
BglII AGATCT 3 cut(s) 505, 747, 769
BisI GCNGC 3 cut(s) 201, 317, 516
BlpI GCTNAGC 1 cut(s) 797
BlsI GCNGC 3 cut(s) 202, 318, 517
Bme1390I CCNGG 2 cut(s) 408, 722
Bme18I GGWCC 1 cut(s) 387
BmgT120I GGNCC 1 cut(s) 387
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 408, 722
BpiI GAAGAC 1 cut(s) 311
BpmI CTGGAG 2 cut(s) 429, 507
Bpu10I CCTNAGC 1 cut(s) 21
Bpu1102I GCTNAGC 1 cut(s) 797
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 415
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 3 cut(s) 211, 490, 673
BseBI CCWGG 2 cut(s) 408, 722
BseDI CCNNGG 1 cut(s) 415
BseGI GGATG 1 cut(s) 614
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMII CTCAG 4 cut(s) 35, 86, 204, 405
BseNI ACTGG 3 cut(s) 211, 490, 673
BseRI GAGGAG 3 cut(s) 647, 717, 758
BseXI GCAGC 2 cut(s) 187, 303
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 186
BslFI GGGAC 1 cut(s) 769
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 2 cut(s) 405, 629
BsmFI GGGAC 1 cut(s) 769
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 3 cut(s) 505, 747, 769
Bsp1720I GCTNAGC 1 cut(s) 797
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 1 cut(s) 515
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 34, 85, 205, 404
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 405
BspMAI CTGCAG 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 405
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 3 cut(s) 211, 490, 673
BssECI CCNNGG 1 cut(s) 415
BssMI GATC 3 cut(s) 505, 747, 769
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 2 cut(s) 408, 722
Bst4CI ACNGT 6 cut(s) 161, 177, 289, 310, 330, 582
Bst6I CTCTTC 1 cut(s) 88
BstAUI TGTACA 1 cut(s) 346
BstDEI CTNAG 7 cut(s) 21, 72, 213, 391, 430, 731, 797
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 1 cut(s) 614
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 3 cut(s) 508, 750, 772
BstMAI GTCTC 2 cut(s) 405, 629
BstMBI GATC 3 cut(s) 505, 747, 769
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstNI CCWGG 2 cut(s) 408, 722
BstSCI CCNGG 2 cut(s) 406, 720
BstSFI CTRYAG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 187, 303
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 3 cut(s) 505, 747, 769
BstYI RGATCY 3 cut(s) 505, 747, 769
BsuI GTATCC 1 cut(s) 37
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 1 cut(s) 614
BtsIMutI CAGTG 1 cut(s) 587
CciI TCATGA 1 cut(s) 355
CfoI GCGC 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 387
Csp6I GTAC 2 cut(s) 210, 347
CviAII CATG 3 cut(s) 42, 356, 416
CviQI GTAC 2 cut(s) 210, 347
DdeI CTNAG 7 cut(s) 21, 72, 213, 391, 430, 731, 797
DpnI GATC 3 cut(s) 507, 749, 771
DpnII GATC 3 cut(s) 505, 747, 769
DraI TTTAAA 1 cut(s) 277
DraIII CACNNNGTG 1 cut(s) 582
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 387
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 406, 720
EcoT14I CCWWGG 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 3 cut(s) 45, 359, 419
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 769
FatI CATG 3 cut(s) 41, 355, 415
FauNDI CATATG 1 cut(s) 238
Fnu4HI GCNGC 3 cut(s) 201, 317, 516
FokI GGATG 1 cut(s) 621
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 201, 317, 516
FspBI CTAG 4 cut(s) 296, 384, 570, 814
GlaI GCGC 1 cut(s) 77
GluI GCNGC 3 cut(s) 201, 317, 516
GsuI CTGGAG 2 cut(s) 429, 507
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 78
Hin1II CATG 3 cut(s) 45, 359, 419
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HincII GTYRAC 1 cut(s) 602
HindII GTYRAC 1 cut(s) 602
HinfI GANTC 4 cut(s) 145, 233, 566, 622
HphI GGTGA 2 cut(s) 383, 455
Hpy166II GTNNAC 4 cut(s) 155, 244, 461, 602
Hpy188I TCNGA 2 cut(s) 224, 510
Hpy188III TCNNGA 6 cut(s) 13, 166, 283, 356, 570, 626
Hpy8I GTNNAC 4 cut(s) 155, 244, 461, 602
HpyAV CCTTC 1 cut(s) 10
HpyCH4III ACNGT 6 cut(s) 161, 177, 289, 310, 330, 582
HpyCH4IV ACGT 2 cut(s) 252, 604
HpyCH4V TGCA 3 cut(s) 56, 203, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpyF3I CTNAG 7 cut(s) 21, 72, 213, 391, 430, 731, 797
HpySE526I ACGT 2 cut(s) 252, 604
Hsp92II CATG 3 cut(s) 45, 359, 419
HspAI GCGC 1 cut(s) 76
Kzo9I GATC 3 cut(s) 505, 747, 769
LmnI GCTCC 2 cut(s) 189, 292
Lsp1109I GCAGC 2 cut(s) 187, 303
MaeI CTAG 4 cut(s) 296, 384, 570, 814
MaeII ACGT 2 cut(s) 252, 604
MaeIII GTNAC 2 cut(s) 615, 803
MalI GATC 3 cut(s) 507, 749, 771
MboI GATC 3 cut(s) 505, 747, 769
MboII GAAGA 2 cut(s) 105, 316
MflI RGATCY 3 cut(s) 505, 747, 769
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 4 cut(s) 129, 446, 452, 464
MseI TTAA 2 cut(s) 276, 476
MspA1I CMGCKG 1 cut(s) 695
MspR9I CCNGG 2 cut(s) 408, 722
MvaI CCWGG 2 cut(s) 408, 722
MwoI GCNNNNNNNGC 1 cut(s) 255
NcoI CCATGG 1 cut(s) 415
NdeI CATATG 1 cut(s) 238
NdeII GATC 3 cut(s) 505, 747, 769
NlaIII CATG 3 cut(s) 45, 359, 419
NlaIV GGNNCC 1 cut(s) 405
NmeAIII GCCGAG 1 cut(s) 629
PagI TCATGA 1 cut(s) 355
PfeI GAWTC 4 cut(s) 145, 233, 566, 622
PkrI GCNGC 3 cut(s) 202, 318, 517
PpuMI RGGWCCY 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 387
Psp6I CCWGG 2 cut(s) 406, 720
PspGI CCWGG 2 cut(s) 406, 720
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 387
PspPPI RGGWCCY 1 cut(s) 387
PstI CTGCAG 1 cut(s) 321
PsuI RGATCY 3 cut(s) 505, 747, 769
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 211, 348
RsaNI GTAC 2 cut(s) 210, 347
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 276, 476
SatI GCNGC 3 cut(s) 201, 317, 516
Sau3AI GATC 3 cut(s) 505, 747, 769
Sau96I GGNCC 1 cut(s) 387
ScrFI CCNGG 2 cut(s) 408, 722
SduI GDGCHC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 317
SinI GGWCC 1 cut(s) 387
Sse9I AATT 4 cut(s) 129, 446, 452, 464
SsiI CCGC 1 cut(s) 515
SspI AATATT 3 cut(s) 474, 542, 659
SspMI CTAG 4 cut(s) 296, 384, 570, 814
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 2 cut(s) 406, 720
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 6 cut(s) 161, 177, 289, 310, 330, 582
TaiI ACGT 2 cut(s) 255, 607
TaqI TCGA 1 cut(s) 165
TasI AATT 4 cut(s) 129, 446, 452, 464
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 4 cut(s) 145, 233, 566, 622
Tru1I TTAA 2 cut(s) 276, 476
Tru9I TTAA 2 cut(s) 276, 476
TscAI CASTG 1 cut(s) 587
TseI GCWGC 2 cut(s) 200, 316
TspDTI ATGAA 4 cut(s) 184, 225, 344, 509
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 387
XapI RAATTY 2 cut(s) 129, 452
XbaI TCTAGA 1 cut(s) 569
XspI CTAG 4 cut(s) 296, 384, 570, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.