Rorug02G0234700
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015736
Physical Location & Seq
Reverse (-)
23420813 .. 23422292
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0234700.1

Sequence Viewer

Length: 1218 bp
ATGCATTCAGATACTGCATATCATGCCAGTACTTCATTCCTTGGAGGAGTCAATGCAGACGAGTTACTCAATTCCCTGGGGCCTCCTTGCCATATGAATTTGGATAAACAAAATGATTCTTCTGTGCATAGGCACTCTGATAAGCAAAATGACCCTCGAATATTGAAGAATCTTGACAGTTCTGATCAATGTATGAATACAGAACTAGGAGACCTGACTGAGCTACAGATGTCCAGTTCTAGGGACCACTTCAGTACTATAGTAAGTGAGAATGGGAATGATGGGTCCGGTGCTCTTCAGCCAGTTCAAATAGCTATTCCAGGACCTTCTCTATTCCCGGACCTTCTCTTTAACATTATTGATCTTTCACCAAACTGGGCATATGAAAACTCTGAAACAGAGGTTTTTGTTGTGGGAAAGTTCTTGAAGAACCAAGAATTAGAAAGTTTTAAGTGGTCATGTATGTTTGGGGAAGTAGAAGTCCCCGCTGAGGTTGTAGCAGACGGTGTTCTTCGTTGTTATACGCCCGTACATAGGGCTGCTATGATTCCTTTCTATGTAACACGTTCCAATAGGTTAGCGTGTAGTGAAGTGAGAATATTTGTGTACCGAGACAACCAAATTCAAGATGCGGATTTTAAGGATGACGGCTGCGAAGACGATACACTTGTTATGCGTTTTGGAAGAATACTGTCCCCAAGCTTTACGTATTCAAGTTTTAATCCACAGGATAAATATAGACAATATGATCCTATCAGTGTAGTTGAGAATTCTGATTTGACTAGTAAAATTAGTTCATTGCTAAAAAACGGCAATGAGGAAAGAGCTGAGATGATAAAGCTATTCCCAGATGAGTTTTCCTTGGATAGAGTAAAGGCGCAGTTGCTTCAACAGTTTCTTAAAGACAAGTTTCATGAATGGCTCTTGCAGAAGCTGGCTGTTGGTGGAAATGGCTTAGGGGTACTAGACGAAGGTGGCCAGGGTGTGTTACATGTTGGAGCTGCTCTTGGCTATGACTGGGTCTTTTCACCCATGACAATGGCTGGAGTTACTGTCAATTTTCCTGATGTGAATGGATGGACGGCACTTCATTGGGCAGCTTTTCGTGGCAGGGTGATTGTAGGAACTAACAGAGTGATTGTGGGAAGAAGAGGCCCTAATTGGGCTCTTAAAAATATGGTAAACTATGCAGTGGATATCCCAGGGAAAGTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

405

Amino Acids

45.21

Weight (kDa)

5.12

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 486, 632
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 3 cut(s) 97, 621, 769
AcuI CTGAAG 2 cut(s) 235, 281
AfaI GTAC 5 cut(s) 31, 256, 531, 608, 963
AfiI CCNNNNNNNGG 4 cut(s) 240, 490, 534, 1162
AflIII ACRYGT 2 cut(s) 563, 991
AgsI TTSAA 6 cut(s) 166, 308, 427, 626, 714, 890
AhlI ACTAGT 1 cut(s) 782
AjnI CCWGG 4 cut(s) 75, 319, 978, 1201
AluBI AGCT 8 cut(s) 223, 314, 702, 827, 841, 934, 1001, 1100
AluI AGCT 8 cut(s) 223, 314, 702, 827, 841, 934, 1001, 1100
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 2 cut(s) 204, 606
AlwI GGATC 1 cut(s) 743
AlwNI CAGNNNCTG 2 cut(s) 14, 934
AoxI GGCC 3 cut(s) 80, 976, 1153
ApeKI GCWGC 4 cut(s) 539, 651, 1001, 1097
ApoI RAATTY 3 cut(s) 97, 621, 769
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 6 cut(s) 80, 244, 285, 323, 340, 1154
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 360, 1020, 1126
AvaII GGWCC 4 cut(s) 244, 285, 323, 340
BaeI ACNNNNGTAYC 2 cut(s) 654, 687
BalI TGGCCA 1 cut(s) 978
BanII GRGCYC 1 cut(s) 1168
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 489
BbvI GCAGC 4 cut(s) 526, 638, 988, 1109
BccI CCATC 2 cut(s) 275, 1071
BceAI ACGGC 3 cut(s) 664, 826, 1098
BciT130I CCWGG 4 cut(s) 77, 321, 980, 1203
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 204, 606
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 4 cut(s) 206, 240, 783, 965
BfmI CTRYAG 2 cut(s) 224, 258
BisI GCNGC 4 cut(s) 540, 652, 1002, 1098
BlsI GCNGC 4 cut(s) 541, 653, 1003, 1099
BmcAI AGTACT 2 cut(s) 31, 256
Bme1390I CCNGG 5 cut(s) 77, 321, 338, 980, 1203
Bme18I GGWCC 4 cut(s) 244, 285, 323, 340
BmgT120I GGNCC 6 cut(s) 80, 244, 285, 323, 340, 1154
BmiI GGNNCC 3 cut(s) 81, 245, 286
BmrFI CCNGG 5 cut(s) 77, 321, 338, 980, 1203
BmrI ACTGGG 2 cut(s) 385, 1027
BmsI GCATC 1 cut(s) 619
BmuI ACTGGG 2 cut(s) 385, 1027
BpiI GAAGAC 1 cut(s) 663
BpmI CTGGAG 1 cut(s) 1065
Bpu10I CCTNAGC 2 cut(s) 489, 955
BpuMI CCSGG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 7 cut(s) 40, 75, 76, 861, 979, 1201, 1202
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 1038, 1068
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 490, 534, 1162
Bse1I ACTGG 5 cut(s) 27, 234, 302, 380, 1022
Bse3DI GCAATG 2 cut(s) 797, 820
BseBI CCWGG 4 cut(s) 77, 321, 980, 1203
BseDI CCNNGG 7 cut(s) 40, 75, 76, 861, 979, 1201, 1202
BseGI GGATG 2 cut(s) 649, 1082
BseLI CCNNNNNNNGG 4 cut(s) 240, 490, 534, 1162
BseMI GCAATG 2 cut(s) 797, 820
BseMII CTCAG 3 cut(s) 210, 480, 819
BseNI ACTGG 5 cut(s) 27, 234, 302, 380, 1022
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 4 cut(s) 526, 638, 988, 1109
BshFI GGCC 3 cut(s) 82, 978, 1155
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 2 cut(s) 288, 338
BslFI GGGAC 3 cut(s) 257, 467, 679
BslI CCNNNNNNNGG 4 cut(s) 240, 490, 534, 1162
BsmAI GTCTC 2 cut(s) 204, 606
BsmFI GGGAC 3 cut(s) 257, 467, 679
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 3 cut(s) 82, 978, 1155
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 295, 1168
Bsp143I GATC 3 cut(s) 184, 361, 748
BspACI CCGC 2 cut(s) 486, 632
BspANI GGCC 3 cut(s) 82, 978, 1155
BspCNI CTCAG 3 cut(s) 211, 481, 820
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 3 cut(s) 81, 245, 286
BspPI GGATC 1 cut(s) 743
BspQI GCTCTTC 1 cut(s) 300
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 2 cut(s) 797, 820
BsrI ACTGG 5 cut(s) 27, 234, 302, 380, 1022
BssECI CCNNGG 7 cut(s) 40, 75, 76, 861, 979, 1201, 1202
BssMI GATC 3 cut(s) 184, 361, 748
BssT1I CCWWGG 2 cut(s) 40, 861
Bst2UI CCWGG 4 cut(s) 77, 321, 980, 1203
Bst4CI ACNGT 5 cut(s) 179, 506, 693, 894, 1054
Bst6I CTCTTC 2 cut(s) 300, 1144
BstAPI GCANNNNNTGC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 708
BstC8I GCNNGC 1 cut(s) 936
BstDEI CTNAG 4 cut(s) 219, 489, 828, 955
BstF5I GGATG 2 cut(s) 649, 1082
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 3 cut(s) 187, 364, 751
BstMAI GTCTC 2 cut(s) 204, 606
BstMBI GATC 3 cut(s) 184, 361, 748
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 4 cut(s) 77, 321, 980, 1203
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 5 cut(s) 75, 319, 336, 978, 1201
BstSFI CTRYAG 2 cut(s) 224, 258
BstSNI TACGTA 1 cut(s) 708
BstV1I GCAGC 4 cut(s) 526, 638, 988, 1109
BstV2I GAAGAC 1 cut(s) 663
BstXI CCANNNNNNTGG 1 cut(s) 1039
BsuRI GGCC 3 cut(s) 82, 978, 1155
BtsCI GGATG 2 cut(s) 649, 1082
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 2 cut(s) 763, 1197
Cac8I GCNNGC 1 cut(s) 936
CaiI CAGNNNCTG 2 cut(s) 14, 934
CciI TCATGA 1 cut(s) 913
CfoI GCGC 1 cut(s) 880
Cfr13I GGNCC 6 cut(s) 80, 244, 285, 323, 340, 1154
Csp6I GTAC 5 cut(s) 30, 255, 530, 607, 962
CviAII CATG 5 cut(s) 23, 459, 914, 992, 1033
CviQI GTAC 5 cut(s) 30, 255, 530, 607, 962
DdeI CTNAG 4 cut(s) 219, 489, 828, 955
DpnI GATC 3 cut(s) 186, 363, 750
DpnII GATC 3 cut(s) 184, 361, 748
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 2 cut(s) 300, 1144
EarI CTCTTC 2 cut(s) 300, 1144
Eco105I TACGTA 1 cut(s) 708
Eco130I CCWWGG 2 cut(s) 40, 861
Eco24I GRGCYC 1 cut(s) 1168
Eco31I GGTCTC 1 cut(s) 204
Eco32I GATATC 1 cut(s) 1198
Eco47I GGWCC 4 cut(s) 244, 285, 323, 340
Eco57I CTGAAG 2 cut(s) 235, 281
EcoO109I RGGNCCY 3 cut(s) 80, 323, 1154
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 4 cut(s) 75, 319, 978, 1201
EcoRV GATATC 1 cut(s) 1198
EcoT14I CCWWGG 2 cut(s) 40, 861
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1168
ErhI CCWWGG 2 cut(s) 40, 861
FaeI CATG 5 cut(s) 26, 462, 917, 995, 1036
FaqI GGGAC 3 cut(s) 257, 467, 679
FatI CATG 5 cut(s) 22, 458, 913, 991, 1032
FauI CCCGC 1 cut(s) 493
FauNDI CATATG 2 cut(s) 93, 382
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 4 cut(s) 540, 652, 1002, 1098
FokI GGATG 2 cut(s) 656, 1089
FriOI GRGCYC 1 cut(s) 1168
Fsp4HI GCNGC 4 cut(s) 540, 652, 1002, 1098
FspBI CTAG 4 cut(s) 206, 240, 783, 965
GlaI GCGC 1 cut(s) 879
GluI GCNGC 4 cut(s) 540, 652, 1002, 1098
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 3 cut(s) 82, 978, 1155
HapII CCGG 2 cut(s) 288, 338
HhaI GCGC 1 cut(s) 880
Hin1II CATG 5 cut(s) 26, 462, 917, 995, 1036
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 4 cut(s) 48, 116, 169, 547
HpaII CCGG 2 cut(s) 288, 338
HphI GGTGA 3 cut(s) 360, 1020, 1126
Hpy166II GTNNAC 2 cut(s) 607, 1183
Hpy188I TCNGA 5 cut(s) 10, 139, 184, 394, 775
Hpy188III TCNNGA 5 cut(s) 173, 424, 626, 914, 1064
Hpy8I GTNNAC 2 cut(s) 607, 1183
HpyAV CCTTC 3 cut(s) 336, 353, 965
HpyCH4III ACNGT 5 cut(s) 179, 506, 693, 894, 1054
HpyCH4IV ACGT 2 cut(s) 565, 707
HpyCH4V TGCA 6 cut(s) 4, 17, 56, 127, 928, 1190
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 4 cut(s) 219, 489, 828, 955
HpySE526I ACGT 2 cut(s) 565, 707
Hsp92II CATG 5 cut(s) 26, 462, 917, 995, 1036
HspAI GCGC 1 cut(s) 878
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 184, 361, 748
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 1 cut(s) 998
Lsp1109I GCAGC 4 cut(s) 526, 638, 988, 1109
LweI GCATC 1 cut(s) 619
MaeI CTAG 4 cut(s) 206, 240, 783, 965
MaeII ACGT 2 cut(s) 565, 707
MaeIII GTNAC 4 cut(s) 63, 559, 987, 1048
MalI GATC 3 cut(s) 186, 363, 750
MboI GATC 3 cut(s) 184, 361, 748
MboII GAAGA 9 cut(s) 111, 178, 287, 439, 503, 668, 696, 1158, 1161
MhlI GDGCHC 2 cut(s) 295, 1168
MlsI TGGCCA 1 cut(s) 978
MluCI AATT 8 cut(s) 70, 97, 437, 621, 769, 789, 1057, 1159
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 976
MnlI CCTC 7 cut(s) 38, 93, 165, 394, 484, 811, 1145
Mox20I TGGCCA 1 cut(s) 978
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 978
MseI TTAA 6 cut(s) 351, 450, 639, 720, 900, 1170
MslI CAYNNNNRTG 1 cut(s) 1037
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 2 cut(s) 288, 338
MspR9I CCNGG 5 cut(s) 77, 321, 338, 980, 1203
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 4 cut(s) 77, 321, 980, 1203
MwoI GCNNNNNNNGC 1 cut(s) 23
NciI CCSGG 1 cut(s) 338
NdeI CATATG 2 cut(s) 93, 382
NdeII GATC 3 cut(s) 184, 361, 748
NlaIII CATG 5 cut(s) 26, 462, 917, 995, 1036
NlaIV GGNNCC 3 cut(s) 81, 245, 286
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 995
PagI TCATGA 1 cut(s) 913
PasI CCCWGGG 2 cut(s) 76, 1202
PciI ACATGT 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 300
PctI GAATGC 1 cut(s) 4
PfeI GAWTC 3 cut(s) 116, 169, 547
PflFI GACNNNGTC 1 cut(s) 1019
PfoI TCCNGGA 2 cut(s) 319, 336
PkrI GCNGC 4 cut(s) 541, 653, 1003, 1099
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 708
PpuMI RGGWCCY 1 cut(s) 323
PscI ACATGT 1 cut(s) 991
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 4 cut(s) 75, 319, 978, 1201
PspGI CCWGG 4 cut(s) 75, 319, 978, 1201
PspN4I GGNNCC 3 cut(s) 81, 245, 286
PspPI GGNCC 6 cut(s) 80, 244, 285, 323, 340, 1154
PspPPI RGGWCCY 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 778, 810
PstNI CAGNNNCTG 2 cut(s) 14, 934
PsyI GACNNNGTC 1 cut(s) 1019
RsaI GTAC 5 cut(s) 31, 256, 531, 608, 963
RsaNI GTAC 5 cut(s) 30, 255, 530, 607, 962
RseI CAYNNNNRTG 1 cut(s) 1037
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 6 cut(s) 351, 450, 639, 720, 900, 1170
SatI GCNGC 4 cut(s) 540, 652, 1002, 1098
Sau3AI GATC 3 cut(s) 184, 361, 748
Sau96I GGNCC 6 cut(s) 80, 244, 285, 323, 340, 1154
ScaI AGTACT 2 cut(s) 31, 256
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 5 cut(s) 77, 321, 338, 980, 1203
SduI GDGCHC 2 cut(s) 295, 1168
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 2 cut(s) 224, 258
SinI GGWCC 4 cut(s) 244, 285, 323, 340
SmiMI CAYNNNNRTG 1 cut(s) 1037
SnaBI TACGTA 1 cut(s) 708
SpeI ACTAGT 1 cut(s) 782
Sse9I AATT 8 cut(s) 70, 97, 437, 621, 769, 789, 1057, 1159
SsiI CCGC 2 cut(s) 486, 632
SspI AATATT 2 cut(s) 162, 600
SspMI CTAG 4 cut(s) 206, 240, 783, 965
StyD4I CCNGG 5 cut(s) 75, 319, 336, 978, 1201
StyI CCWWGG 2 cut(s) 40, 861
TaaI ACNGT 5 cut(s) 179, 506, 693, 894, 1054
TaiI ACGT 2 cut(s) 568, 710
TaqI TCGA 1 cut(s) 157
TasI AATT 8 cut(s) 70, 97, 437, 621, 769, 789, 1057, 1159
TatI WGTACW 2 cut(s) 29, 254
TfiI GAWTC 3 cut(s) 116, 169, 547
Tru1I TTAA 6 cut(s) 351, 450, 639, 720, 900, 1170
Tru9I TTAA 6 cut(s) 351, 450, 639, 720, 900, 1170
TscAI CASTG 2 cut(s) 763, 1197
TseI GCWGC 4 cut(s) 539, 651, 1001, 1097
TspDTI ATGAA 8 cut(s) 24, 110, 209, 399, 786, 902, 930, 1079
TspRI CASTG 2 cut(s) 763, 1197
Tth111I GACNNNGTC 1 cut(s) 1019
VpaK11BI GGWCC 4 cut(s) 244, 285, 323, 340
XapI RAATTY 3 cut(s) 97, 621, 769
XceI RCATGY 1 cut(s) 995
XspI CTAG 4 cut(s) 206, 240, 783, 965
ZrmI AGTACT 2 cut(s) 31, 256
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.