Rh2DG276000

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015736
Physical Location & Seq
Forward (+)
30844262 .. 30847740
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG276000.1

Sequence Viewer

Length: 996 bp
ATGAGTGCTATAGATTTTCTCTCTCCATCACCTCCTTCAACTATTTTCCCCTCTCGATCTGTCATATTCTCTGCGGAGTGCATGCAGCCCCATCCCCTCGTTCTTACTTCCCTCTTTTCCCTTTATATACCTTCCGATCCCCTCCCTCCACTCCTCCACTTTCATAGCAGATTGGTCAAACTCCAACCAACTTTCTACCATTTTCAGATCGCTTCGTTTTCTTCATCGGAATCCATGGCGAAACAACCAGCTCGCGTCCTCGTTACCAGAGCCACAGGTGAAATCGGGTATGCTCTTGTACCTATGATCGCAAAAGGAGTGATGCTCGGAGCTGACCAGCCCGTGATCCTTCACCTTCTTGATGAACCATTCACTGCTGAAGCATTGAATGGTGTCAAGATGGAGTTGATCGATGCTGCTTTCCCTCTTCTCAAAGGTGTTGTTGCTACATCTGATGTTGTGAAGGCATGCACTGGAGTCAACATTGCAGTCATAGTTGGTGGCTTCCCAGGGAAAGAGGGCATGGTAAAGAAAGATGTCCTTTCAGAGAAAGATGCTATCTCCATTCGCGGAGCCGCAGTTCGCGAGAGCAAAAACGTCAAGCTCTTAGACCCACTTATAAGTTCTCCATCAAAAGCCGTCTGCATTTCTCCATATCAGAGACTGTCTGCTGATGTTCCTGTACAACTTCATGAGAGGTTTCAAGGTGAGAGTATCACTAGGTCCTCAGTTATAGAGGAACCTGGAGACCATGGAGAAGCACACTTAGTAGCTCTGGGTGAATTAGAATTCCGTAAACAATTTCTAATATTAAGTGATGCTGGAGGAATGAAGTTGAAAGATTTGATAGCGGAAGAGGACATTAGGCATTGGAAATATTTGCCAATGGTAACAGTTTTGATTGGGATTCTGGAAGCACACAGTGTCTTGGGTTGTTACGTCAACGTGGATGGGAGTTACAGATTCAAGGATTACTCGCAGCAGCCCCCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04964 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200 map04964
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

331

Amino Acids

36.06

Weight (kDa)

6.07

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 85 - 187 2.9e-10 lactate/malate dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 620
AccII CGCG 3 cut(s) 255, 570, 585
AciI CCGC 4 cut(s) 74, 570, 576, 851
AclWI GGATC 2 cut(s) 131, 340
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 1 cut(s) 399
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 2 cut(s) 300, 684
AgsI TTSAA 5 cut(s) 39, 388, 704, 838, 967
AjnI CCWGG 2 cut(s) 508, 742
AloI GAACNNNNNNTCC 2 cut(s) 564, 596
AluBI AGCT 4 cut(s) 251, 332, 604, 773
AluI AGCT 4 cut(s) 251, 332, 604, 773
Alw26I GTCTC 2 cut(s) 655, 741
AlwI GGATC 2 cut(s) 131, 340
AlwNI CAGNNNCTG 1 cut(s) 664
ApeKI GCWGC 4 cut(s) 85, 416, 979, 982
ApoI RAATTY 1 cut(s) 788
AspS9I GGNCC 1 cut(s) 723
AsuHPI GGTGA 5 cut(s) 21, 290, 344, 719, 791
AvaII GGWCC 1 cut(s) 723
BbvI GCAGC 3 cut(s) 97, 403, 991
BccI CCATC 5 cut(s) 34, 99, 394, 637, 944
BceAI ACGGC 1 cut(s) 623
BciT130I CCWGG 2 cut(s) 510, 744
BcoDI GTCTC 2 cut(s) 655, 741
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 5 cut(s) 86, 417, 576, 980, 983
BlsI GCNGC 5 cut(s) 87, 418, 577, 981, 984
Bme1390I CCNGG 2 cut(s) 510, 744
Bme18I GGWCC 1 cut(s) 723
BmgT120I GGNCC 1 cut(s) 723
BmiI GGNNCC 2 cut(s) 574, 741
BmrFI CCNGG 2 cut(s) 510, 744
BmsI GCATC 4 cut(s) 312, 403, 544, 808
BplI GAGNNNNNCTC 2 cut(s) 309, 341
BpmI CTGGAG 3 cut(s) 495, 765, 843
Bsa29I ATCGAT 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 4 cut(s) 234, 508, 509, 751
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 2 cut(s) 510, 744
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 4 cut(s) 234, 508, 509, 751
BseGI GGATG 2 cut(s) 91, 955
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 3 cut(s) 97, 403, 991
Bsh1236I CGCG 3 cut(s) 255, 570, 585
BshVI ATCGAT 1 cut(s) 411
BsmAI GTCTC 2 cut(s) 655, 741
Bso31I GGTCTC 1 cut(s) 741
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 6 cut(s) 56, 136, 207, 306, 345, 408
Bsp19I CCATGG 2 cut(s) 234, 751
Bsp68I TCGCGA 1 cut(s) 585
BspACI CCGC 4 cut(s) 74, 570, 576, 851
BspCNI CTCAG 1 cut(s) 740
BspDI ATCGAT 1 cut(s) 411
BspFNI CGCG 3 cut(s) 255, 570, 585
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 2 cut(s) 574, 741
BspPI GGATC 2 cut(s) 131, 340
BspTNI GGTCTC 1 cut(s) 741
BsrDI GCAATG 1 cut(s) 483
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 4 cut(s) 234, 508, 509, 751
BssMI GATC 6 cut(s) 56, 136, 207, 306, 345, 408
BssT1I CCWWGG 2 cut(s) 234, 751
Bst2UI CCWGG 2 cut(s) 510, 744
Bst4CI ACNGT 3 cut(s) 666, 895, 923
Bst6I CTCTTC 2 cut(s) 432, 849
BstAUI TGTACA 1 cut(s) 682
BstC8I GCNNGC 3 cut(s) 83, 253, 469
BstDEI CTNAG 3 cut(s) 607, 727, 766
BstDSI CCRYGG 2 cut(s) 234, 751
BstF5I GGATG 2 cut(s) 91, 955
BstFNI CGCG 3 cut(s) 255, 570, 585
BstKTI GATC 6 cut(s) 59, 139, 210, 309, 348, 411
BstMAI GTCTC 2 cut(s) 655, 741
BstMBI GATC 6 cut(s) 56, 136, 207, 306, 345, 408
BstNI CCWGG 2 cut(s) 510, 744
BstNSI RCATGY 2 cut(s) 85, 471
BstSCI CCNGG 2 cut(s) 508, 742
BstSFI CTRYAG 1 cut(s) 9
BstUI CGCG 3 cut(s) 255, 570, 585
BstV1I GCAGC 3 cut(s) 97, 403, 991
Bsu15I ATCGAT 1 cut(s) 411
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 2 cut(s) 234, 751
BtsCI GGATG 2 cut(s) 91, 955
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 3 cut(s) 372, 471, 928
BtuMI TCGCGA 1 cut(s) 585
Cac8I GCNNGC 3 cut(s) 83, 253, 469
CaiI CAGNNNCTG 1 cut(s) 664
CciI TCATGA 1 cut(s) 691
Cfr13I GGNCC 1 cut(s) 723
ClaI ATCGAT 1 cut(s) 411
CseI GACGC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 299, 683
CviAII CATG 6 cut(s) 82, 235, 468, 523, 692, 752
CviQI GTAC 2 cut(s) 299, 683
DdeI CTNAG 3 cut(s) 607, 727, 766
DpnI GATC 6 cut(s) 58, 138, 209, 308, 347, 410
DpnII GATC 6 cut(s) 56, 136, 207, 306, 345, 408
DraIII CACNNNGTG 1 cut(s) 923
Eam1104I CTCTTC 2 cut(s) 432, 849
EarI CTCTTC 2 cut(s) 432, 849
Eco130I CCWWGG 2 cut(s) 234, 751
Eco31I GGTCTC 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 723
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 508, 742
EcoT14I CCWWGG 2 cut(s) 234, 751
ErhI CCWWGG 2 cut(s) 234, 751
FaeI CATG 6 cut(s) 85, 238, 471, 526, 695, 755
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FatI CATG 6 cut(s) 81, 234, 467, 522, 691, 751
Fnu4HI GCNGC 5 cut(s) 86, 417, 576, 980, 983
FokI GGATG 2 cut(s) 78, 962
Fsp4HI GCNGC 5 cut(s) 86, 417, 576, 980, 983
FspBI CTAG 1 cut(s) 720
GluI GCNGC 5 cut(s) 86, 417, 576, 980, 983
GsuI CTGGAG 3 cut(s) 495, 765, 843
HgaI GACGC 1 cut(s) 244
Hin1II CATG 6 cut(s) 85, 238, 471, 526, 695, 755
HincII GTYRAC 2 cut(s) 481, 943
HindII GTYRAC 2 cut(s) 481, 943
HinfI GANTC 4 cut(s) 230, 477, 907, 963
HphI GGTGA 5 cut(s) 21, 290, 344, 719, 791
Hpy166II GTNNAC 3 cut(s) 481, 797, 943
Hpy188I TCNGA 7 cut(s) 136, 207, 229, 329, 454, 547, 660
Hpy188III TCNNGA 6 cut(s) 54, 359, 397, 584, 692, 911
Hpy8I GTNNAC 3 cut(s) 481, 797, 943
HpyAV CCTTC 5 cut(s) 45, 141, 359, 365, 457
HpyCH4III ACNGT 3 cut(s) 666, 895, 923
HpyCH4IV ACGT 3 cut(s) 597, 939, 945
HpyCH4V TGCA 5 cut(s) 81, 85, 471, 488, 645
HpyF3I CTNAG 3 cut(s) 607, 727, 766
HpySE526I ACGT 3 cut(s) 597, 939, 945
Hsp92II CATG 6 cut(s) 85, 238, 471, 526, 695, 755
Kzo9I GATC 6 cut(s) 56, 136, 207, 306, 345, 408
LmnI GCTCC 2 cut(s) 329, 572
Lsp1109I GCAGC 3 cut(s) 97, 403, 991
LweI GCATC 4 cut(s) 312, 403, 544, 808
MaeI CTAG 1 cut(s) 720
MaeII ACGT 3 cut(s) 597, 939, 945
MaeIII GTNAC 4 cut(s) 262, 889, 935, 956
MalI GATC 6 cut(s) 58, 138, 209, 308, 347, 410
MboI GATC 6 cut(s) 56, 136, 207, 306, 345, 408
MboII GAAGA 3 cut(s) 213, 419, 866
MluCI AATT 3 cut(s) 782, 788, 800
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 1 cut(s) 208
MseI TTAA 1 cut(s) 812
MspR9I CCNGG 2 cut(s) 510, 744
MvaI CCWGG 2 cut(s) 510, 744
MvnI CGCG 3 cut(s) 255, 570, 585
NcoI CCATGG 2 cut(s) 234, 751
NdeII GATC 6 cut(s) 56, 136, 207, 306, 345, 408
NlaIII CATG 6 cut(s) 85, 238, 471, 526, 695, 755
NlaIV GGNNCC 2 cut(s) 574, 741
NruI TCGCGA 1 cut(s) 585
NspI RCATGY 2 cut(s) 85, 471
PaeI GCATGC 2 cut(s) 85, 471
PagI TCATGA 1 cut(s) 691
PasI CCCWGGG 1 cut(s) 509
PfeI GAWTC 3 cut(s) 230, 907, 963
PkrI GCNGC 5 cut(s) 87, 418, 577, 981, 984
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 723
PsiI TTATAA 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 508, 742
PspGI CCWGG 2 cut(s) 508, 742
PspN4I GGNNCC 2 cut(s) 574, 741
PspPI GGNCC 1 cut(s) 723
PspPPI RGGWCCY 1 cut(s) 723
PstNI CAGNNNCTG 1 cut(s) 664
RruI TCGCGA 1 cut(s) 585
RsaI GTAC 2 cut(s) 300, 684
RsaNI GTAC 2 cut(s) 299, 683
SaqAI TTAA 1 cut(s) 812
SatI GCNGC 5 cut(s) 86, 417, 576, 980, 983
Sau3AI GATC 6 cut(s) 56, 136, 207, 306, 345, 408
Sau96I GGNCC 1 cut(s) 723
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 2 cut(s) 510, 744
SfaNI GCATC 4 cut(s) 312, 403, 544, 808
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 723
SphI GCATGC 2 cut(s) 85, 471
Sse9I AATT 3 cut(s) 782, 788, 800
SsiI CCGC 4 cut(s) 74, 570, 576, 851
SspI AATATT 2 cut(s) 810, 878
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 508, 742
StyI CCWWGG 2 cut(s) 234, 751
TaaI ACNGT 3 cut(s) 666, 895, 923
TaiI ACGT 3 cut(s) 600, 942, 948
TaqI TCGA 2 cut(s) 55, 411
TasI AATT 3 cut(s) 782, 788, 800
TatI WGTACW 1 cut(s) 682
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 3 cut(s) 230, 907, 963
Tru1I TTAA 1 cut(s) 812
Tru9I TTAA 1 cut(s) 812
TscAI CASTG 3 cut(s) 379, 478, 928
TseI GCWGC 4 cut(s) 85, 416, 979, 982
TspDTI ATGAA 5 cut(s) 152, 213, 378, 680, 845
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 3 cut(s) 379, 478, 928
VpaK11BI GGWCC 1 cut(s) 723
XapI RAATTY 1 cut(s) 788
XceI RCATGY 2 cut(s) 85, 471
XspI CTAG 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.