Rmu_co8427249.1_g000001
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8427249.1
OG0015736
Physical Location & Seq
Reverse (-)
1 .. 1443
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8427249.1_g000001.1.cds

Sequence Viewer

Length: 731 bp
atgagaggtgaggcatttactggtctagagcaacctggaaacaacaataatggacaatcaccttattattcctctggttcatcattgaaatttgaaagtggcacgcacttgttcttgtcaaagcccatctgcttcggtgacgcatttactgacaggtcacttgttgaacaattacctttttcctctggttctccattaaaagccacaagttcttatgaaaggttgtctactcttgcacaagctgatgagctgtttcatggtgaggcatttaatgcctggtcagctgttggacaattaccttgttcctctgtttctccattaaaagccgcagtcacttcttcttgtcaaagattgtctactcctgtacagcttgatgagaggtttcaagttgaggcctttaatgaccggtcacctgttattggaaaatcaccttattcctcttttttgataatgaaagcttttggtgcaggtggggtatttgctgatcagacacctgttcaaggacaatcaccatatgcctcttgttctccatcaaaagccatctgcaaatctccttatcagagaatgtctgctgatgttgttcctgtacaacttcatgaaaggtttcaaggtgagggtatcactaggtcctcagttatagacaaacctggagatcatggagaagcacactttgtagctctgggtgaactagaattccgtaaacaatttctgattttaagttatgctggagg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

244

Amino Acids

26.28

Weight (kDa)

5.96

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 458
AccI GTMKAC 2 cut(s) 227, 356
AciI CCGC 1 cut(s) 327
AcsI RAATTY 2 cut(s) 89, 692
AfaI GTAC 2 cut(s) 366, 588
AfiI CCNNNNNNNGG 2 cut(s) 419, 500
AgeI ACCGGT 1 cut(s) 405
AgsI TTSAA 6 cut(s) 88, 95, 167, 386, 500, 608
AjnI CCWGG 3 cut(s) 34, 275, 646
AloI GAACNNNNNNTCC 2 cut(s) 678, 710
AluBI AGCT 6 cut(s) 242, 250, 284, 370, 458, 677
AluI AGCT 6 cut(s) 242, 250, 284, 370, 458, 677
AoxI GGCC 1 cut(s) 393
ApoI RAATTY 2 cut(s) 89, 692
AsiGI ACCGGT 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 603
AspS9I GGNCC 1 cut(s) 627
AsuHPI GGTGA 9 cut(s) 20, 51, 149, 272, 402, 420, 501, 623, 695
AvaII GGWCC 1 cut(s) 627
BccI CCATC 3 cut(s) 134, 538, 548
BciT130I CCWGG 3 cut(s) 36, 277, 648
BclI TGATCA 1 cut(s) 484
BfaI CTAG 3 cut(s) 26, 624, 689
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
Bme1390I CCNGG 3 cut(s) 36, 277, 648
Bme18I GGWCC 1 cut(s) 627
BmgT120I GGNCC 1 cut(s) 627
BmrFI CCNGG 3 cut(s) 36, 277, 648
BpmI CTGGAG 1 cut(s) 669
BsaWI WCCGGW 1 cut(s) 405
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 500
Bse118I RCCGGY 1 cut(s) 405
Bse1I ACTGG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 36, 277, 648
BseLI CCNNNNNNNGG 2 cut(s) 419, 500
BseMII CTCAG 1 cut(s) 645
BseNI ACTGG 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 486
BshFI GGCC 1 cut(s) 395
BshTI ACCGGT 1 cut(s) 405
BsiSI CCGG 1 cut(s) 406
BslI CCNNNNNNNGG 2 cut(s) 419, 500
BsnI GGCC 1 cut(s) 395
Bsp1407I TGTACA 2 cut(s) 364, 586
Bsp143I GATC 2 cut(s) 484, 652
BspACI CCGC 1 cut(s) 327
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 644
BspHI TCATGA 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 405
BsrGI TGTACA 2 cut(s) 364, 586
BsrI ACTGG 1 cut(s) 25
BssAI RCCGGY 1 cut(s) 405
BssMI GATC 2 cut(s) 484, 652
Bst2UI CCWGG 3 cut(s) 36, 277, 648
BstAPI GCANNNNNTGC 1 cut(s) 272
BstAUI TGTACA 2 cut(s) 364, 586
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 631
BstEII GGTNACC 1 cut(s) 408
BstENI CCTNNNNNAGG 1 cut(s) 498
BstKTI GATC 2 cut(s) 487, 655
BstMBI GATC 2 cut(s) 484, 652
BstMWI GCNNNNNNNGC 3 cut(s) 272, 281, 464
BstNI CCWGG 3 cut(s) 36, 277, 648
BstPI GGTNACC 1 cut(s) 408
BstSCI CCNGG 3 cut(s) 34, 275, 646
BsuRI GGCC 1 cut(s) 395
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 1 cut(s) 104
CciI TCATGA 1 cut(s) 595
Cfr10I RCCGGY 1 cut(s) 405
Cfr13I GGNCC 1 cut(s) 627
CseI GACGC 1 cut(s) 149
Csp6I GTAC 2 cut(s) 365, 587
CspAI ACCGGT 1 cut(s) 405
CviAII CATG 3 cut(s) 257, 596, 656
CviQI GTAC 2 cut(s) 365, 587
DdeI CTNAG 1 cut(s) 631
DpnI GATC 2 cut(s) 486, 654
DpnII GATC 2 cut(s) 484, 652
Eco147I AGGCCT 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 627
Eco91I GGTNACC 1 cut(s) 408
EcoNI CCTNNNNNAGG 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 627
EcoO65I GGTNACC 1 cut(s) 408
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 3 cut(s) 34, 275, 646
FaeI CATG 3 cut(s) 260, 599, 659
FaiI YATR 8 cut(s) 216, 258, 514, 516, 597, 638, 657, 723
FatI CATG 3 cut(s) 256, 595, 655
FauNDI CATATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 2 cut(s) 227, 356
Fnu4HI GCNGC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 3 cut(s) 26, 624, 689
GluI GCNGC 1 cut(s) 327
GsuI CTGGAG 1 cut(s) 669
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 406
HgaI GACGC 1 cut(s) 149
Hin1II CATG 3 cut(s) 260, 599, 659
HindIII AAGCTT 1 cut(s) 456
HpaII CCGG 1 cut(s) 406
HphI GGTGA 9 cut(s) 20, 51, 149, 272, 402, 420, 501, 623, 695
Hpy166II GTNNAC 4 cut(s) 228, 357, 686, 701
Hpy188I TCNGA 3 cut(s) 489, 561, 711
Hpy188III TCNNGA 2 cut(s) 26, 596
Hpy8I GTNNAC 4 cut(s) 228, 357, 686, 701
HpyCH4V TGCA 3 cut(s) 236, 467, 546
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 281, 464
HpyF3I CTNAG 1 cut(s) 631
Hsp92II CATG 3 cut(s) 260, 599, 659
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 2 cut(s) 484, 652
MaeI CTAG 3 cut(s) 26, 624, 689
MaeIII GTNAC 4 cut(s) 137, 156, 331, 408
MalI GATC 2 cut(s) 486, 654
MboI GATC 2 cut(s) 484, 652
MboII GAAGA 1 cut(s) 330
MluCI AATT 5 cut(s) 89, 170, 293, 692, 704
MmeI TCCRAC 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 5 cut(s) 197, 270, 320, 399, 716
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 406
MspR9I CCNGG 3 cut(s) 36, 277, 648
MvaI CCWGG 3 cut(s) 36, 277, 648
MwoI GCNNNNNNNGC 3 cut(s) 272, 281, 464
NdeI CATATG 1 cut(s) 514
NdeII GATC 2 cut(s) 484, 652
NlaIII CATG 3 cut(s) 260, 599, 659
NmuCI GTSAC 4 cut(s) 137, 156, 331, 408
PagI TCATGA 1 cut(s) 595
PaqCI CACCTGC 1 cut(s) 458
PceI AGGCCT 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 603
PinAI ACCGGT 1 cut(s) 405
PkrI GCNGC 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 627
Psp6I CCWGG 3 cut(s) 34, 275, 646
PspEI GGTNACC 1 cut(s) 408
PspGI CCWGG 3 cut(s) 34, 275, 646
PspPI GGNCC 1 cut(s) 627
PspPPI RGGWCCY 1 cut(s) 627
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 2 cut(s) 366, 588
RsaNI GTAC 2 cut(s) 365, 587
SaqAI TTAA 5 cut(s) 197, 270, 320, 399, 716
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 2 cut(s) 484, 652
Sau96I GGNCC 1 cut(s) 627
ScrFI CCNGG 3 cut(s) 36, 277, 648
SinI GGWCC 1 cut(s) 627
Sse9I AATT 5 cut(s) 89, 170, 293, 692, 704
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 1 cut(s) 327
SspMI CTAG 3 cut(s) 26, 624, 689
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 34, 275, 646
TasI AATT 5 cut(s) 89, 170, 293, 692, 704
TatI WGTACW 2 cut(s) 364, 586
TauI GCSGC 1 cut(s) 329
Tru1I TTAA 5 cut(s) 197, 270, 320, 399, 716
Tru9I TTAA 5 cut(s) 197, 270, 320, 399, 716
TseFI GTSAC 4 cut(s) 137, 156, 331, 408
Tsp45I GTSAC 4 cut(s) 137, 156, 331, 408
TspDTI ATGAA 6 cut(s) 69, 231, 245, 467, 584, 612
TspGWI ACGGA 1 cut(s) 686
VpaK11BI GGWCC 1 cut(s) 627
XagI CCTNNNNNAGG 1 cut(s) 498
XapI RAATTY 2 cut(s) 89, 692
XbaI TCTAGA 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 227, 356
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 3 cut(s) 26, 624, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.