Rh3DG074400
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015736
Physical Location & Seq
Forward (+)
5225969 .. 5228395
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG074400.1

Sequence Viewer

Length: 450 bp
ATGGCGAAACAACCAGCTCGCGTCCTCGTTACCAGAGCCACAGGTGAAATCGGGTATGCTCTTGTACCTATGATCGCAAAAGGAGTGATGCTCGGAGCTGACCAGCCCGTGATCCTTCACCTTCTTGATGAACCATTCGCTGCTGAAGCATTGAATGGTGTCAAGATGGAGTTGATCGATGCTGCTTTCCCTCTTCTCAAAGGTGAGAGTATCACTAGGTCCTCAGTTATAGAGGAACCTGGAGACCATGGAGAAGCACGCTTAGTAGCTCTGGGTGCATTAGAATTCCGTAAACAATTTCTAATATTAAGTGATGCTGGAGGAATGAAGTTGAAAGATCTGATAGCGGAAGAGGACATTAGGCATTGGAAATATTTGCCAATGGTAACGTTTGAGAGCAAAATTTGGGAATATCTAGGTCGAAAATGCATCAGCCCTGAAGACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

149

Amino Acids

16.44

Weight (kDa)

5.24

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 21
AciI CCGC 1 cut(s) 347
AclI AACGTT 1 cut(s) 389
AclWI GGATC 1 cut(s) 106
AcsI RAATTY 2 cut(s) 284, 402
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 2 cut(s) 154, 334
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 3 cut(s) 17, 98, 269
AluI AGCT 3 cut(s) 17, 98, 269
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 140, 182
ApoI RAATTY 2 cut(s) 284, 402
AspS9I GGNCC 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 56, 110, 215
AvaII GGWCC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 127, 169
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 237
BfaI CTAG 2 cut(s) 216, 416
BglII AGATCT 1 cut(s) 337
BisI GCNGC 2 cut(s) 141, 183
BlsI GCNGC 2 cut(s) 142, 184
Bme1390I CCNGG 1 cut(s) 240
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 4 cut(s) 78, 169, 304, 438
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 2 cut(s) 261, 339
Bsa29I ATCGAT 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 127, 169
Bsh1236I CGCG 1 cut(s) 21
BshVI ATCGAT 1 cut(s) 177
BsmAI GTCTC 1 cut(s) 237
Bso31I GGTCTC 1 cut(s) 237
Bsp143I GATC 4 cut(s) 72, 111, 174, 337
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 236
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 1 cut(s) 106
BspTNI GGTCTC 1 cut(s) 237
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 4 cut(s) 72, 111, 174, 337
BssT1I CCWWGG 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 2 cut(s) 198, 345
BstC8I GCNNGC 2 cut(s) 19, 259
BstDEI CTNAG 2 cut(s) 223, 262
BstDSI CCRYGG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 4 cut(s) 75, 114, 177, 340
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 4 cut(s) 72, 111, 174, 337
BstMWI GCNNNNNNNGC 2 cut(s) 146, 275
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 127, 169
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 19, 259
Cfr13I GGNCC 1 cut(s) 219
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 10
Csp6I GTAC 1 cut(s) 65
CviAII CATG 1 cut(s) 248
CviJI RGCY 6 cut(s) 17, 38, 98, 106, 269, 435
CviKI_1 RGCY 6 cut(s) 17, 38, 98, 106, 269, 435
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 2 cut(s) 223, 262
DpnI GATC 4 cut(s) 74, 113, 176, 339
DpnII GATC 4 cut(s) 72, 111, 174, 337
Eam1104I CTCTTC 2 cut(s) 198, 345
EarI CTCTTC 2 cut(s) 198, 345
Eco130I CCWWGG 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 219
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 1 cut(s) 251
FaiI YATR 4 cut(s) 57, 71, 230, 249
FatI CATG 1 cut(s) 247
Fnu4HI GCNGC 2 cut(s) 141, 183
Fsp4HI GCNGC 2 cut(s) 141, 183
FspBI CTAG 2 cut(s) 216, 416
GluI GCNGC 2 cut(s) 141, 183
GsuI CTGGAG 2 cut(s) 261, 339
HgaI GACGC 1 cut(s) 10
Hin1II CATG 1 cut(s) 251
HphI GGTGA 3 cut(s) 56, 110, 215
Hpy166II GTNNAC 1 cut(s) 293
Hpy188I TCNGA 2 cut(s) 95, 342
Hpy188III TCNNGA 2 cut(s) 125, 163
Hpy8I GTNNAC 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 125, 131
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 2 cut(s) 278, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 275
HpyF3I CTNAG 2 cut(s) 223, 262
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 1 cut(s) 251
Kzo9I GATC 4 cut(s) 72, 111, 174, 337
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 8 cut(s) 27, 27, 46, 116, 225, 252, 257, 303
Lsp1109I GCAGC 2 cut(s) 127, 169
LweI GCATC 4 cut(s) 78, 169, 304, 438
MaeI CTAG 2 cut(s) 216, 416
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 2 cut(s) 28, 385
MalI GATC 4 cut(s) 74, 113, 176, 339
MboI GATC 4 cut(s) 72, 111, 174, 337
MboII GAAGA 2 cut(s) 185, 362
MflI RGATCY 1 cut(s) 337
MluCI AATT 3 cut(s) 284, 296, 402
MnlI CCTC 6 cut(s) 35, 201, 226, 232, 314, 346
Mph1103I ATGCAT 1 cut(s) 431
MseI TTAA 1 cut(s) 308
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 146, 275
NcoI CCATGG 1 cut(s) 247
NdeII GATC 4 cut(s) 72, 111, 174, 337
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 431
PkrI GCNGC 2 cut(s) 142, 184
PpuMI RGGWCCY 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 219
PspPPI RGGWCCY 1 cut(s) 219
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 2 cut(s) 141, 183
Sau3AI GATC 4 cut(s) 72, 111, 174, 337
Sau96I GGNCC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 240
SfaNI GCATC 4 cut(s) 78, 169, 304, 438
SinI GGWCC 1 cut(s) 219
Sse9I AATT 3 cut(s) 284, 296, 402
SsiI CCGC 1 cut(s) 347
SspI AATATT 2 cut(s) 306, 374
SspMI CTAG 2 cut(s) 216, 416
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 1 cut(s) 392
TaqI TCGA 2 cut(s) 177, 421
TasI AATT 3 cut(s) 284, 296, 402
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TseI GCWGC 2 cut(s) 140, 182
TspDTI ATGAA 2 cut(s) 144, 341
TspGWI ACGGA 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 219
XapI RAATTY 2 cut(s) 284, 402
XspI CTAG 2 cut(s) 216, 416
Zsp2I ATGCAT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.