Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 267, 278 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 526 |
| AciI | CCGC | 4 cut(s) | 311, 335, 649, 721 |
| AcoI | YGGCCR | 2 cut(s) | 528, 646 |
| AfaI | GTAC | 2 cut(s) | 386, 719 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 316, 317, 487, 526, 655 |
| AgsI | TTSAA | 2 cut(s) | 208, 478 |
| AjnI | CCWGG | 1 cut(s) | 274 |
| AleI | CACNNNNGTG | 2 cut(s) | 265, 284 |
| AluBI | AGCT | 2 cut(s) | 188, 241 |
| AluI | AGCT | 2 cut(s) | 188, 241 |
| Alw21I | GWGCWC | 1 cut(s) | 243 |
| Ama87I | CYCGRG | 1 cut(s) | 541 |
| AoxI | GGCC | 4 cut(s) | 390, 528, 577, 646 |
| ApeKI | GCWGC | 2 cut(s) | 185, 337 |
| AspS9I | GGNCC | 3 cut(s) | 78, 390, 577 |
| AsuC2I | CCSGG | 2 cut(s) | 315, 550 |
| AsuHPI | GGTGA | 3 cut(s) | 122, 433, 502 |
| AvaI | CYCGRG | 1 cut(s) | 541 |
| AvaII | GGWCC | 1 cut(s) | 78 |
| BalI | TGGCCA | 1 cut(s) | 530 |
| BanI | GGYRCC | 2 cut(s) | 267, 278 |
| BanII | GRGCYC | 1 cut(s) | 243 |
| BbsI | GAAGAC | 2 cut(s) | 358, 718 |
| Bbv12I | GWGCWC | 1 cut(s) | 243 |
| BbvI | GCAGC | 2 cut(s) | 197, 349 |
| BccI | CCATC | 1 cut(s) | 638 |
| BciT130I | CCWGG | 1 cut(s) | 276 |
| BcnI | CCSGG | 2 cut(s) | 315, 550 |
| BfaI | CTAG | 1 cut(s) | 482 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 276 |
| BisI | GCNGC | 3 cut(s) | 186, 338, 649 |
| BlsI | GCNGC | 3 cut(s) | 187, 339, 650 |
| Bme1390I | CCNGG | 3 cut(s) | 276, 315, 550 |
| Bme18I | GGWCC | 1 cut(s) | 78 |
| BmeT110I | CYCGRG | 1 cut(s) | 541 |
| BmgT120I | GGNCC | 3 cut(s) | 78, 390, 577 |
| BmiI | GGNNCC | 3 cut(s) | 269, 280, 578 |
| BmrFI | CCNGG | 3 cut(s) | 276, 315, 550 |
| BmrI | ACTGGG | 1 cut(s) | 583 |
| BmsI | GCATC | 2 cut(s) | 103, 416 |
| BmuI | ACTGGG | 1 cut(s) | 583 |
| BoxI | GACNNNNGTC | 1 cut(s) | 497 |
| BpiI | GAAGAC | 2 cut(s) | 358, 718 |
| BpmI | CTGGAG | 1 cut(s) | 531 |
| Bpu10I | CCTNAGC | 1 cut(s) | 242 |
| BpuMI | CCSGG | 2 cut(s) | 315, 550 |
| BsaJI | CCNNGG | 3 cut(s) | 163, 274, 314 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 316, 317, 487, 526, 655 |
| Bse1I | ACTGG | 2 cut(s) | 578, 590 |
| BseBI | CCWGG | 1 cut(s) | 276 |
| BseDI | CCNNGG | 3 cut(s) | 163, 274, 314 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 316, 317, 487, 526, 655 |
| BseMII | CTCAG | 3 cut(s) | 256, 371, 395 |
| BseNI | ACTGG | 2 cut(s) | 578, 590 |
| BseRI | GAGGAG | 1 cut(s) | 666 |
| BseXI | GCAGC | 2 cut(s) | 197, 349 |
| BshFI | GGCC | 4 cut(s) | 392, 530, 579, 648 |
| BshNI | GGYRCC | 2 cut(s) | 267, 278 |
| BsiHKAI | GWGCWC | 1 cut(s) | 243 |
| BsiHKCI | CYCGRG | 1 cut(s) | 541 |
| BsiSI | CCGG | 3 cut(s) | 314, 393, 550 |
| BslFI | GGGAC | 3 cut(s) | 136, 187, 386 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 316, 317, 487, 526, 655 |
| BsmFI | GGGAC | 3 cut(s) | 136, 187, 386 |
| BsnI | GGCC | 4 cut(s) | 392, 530, 579, 648 |
| BsoBI | CYCGRG | 1 cut(s) | 541 |
| Bsp1286I | GDGCHC | 1 cut(s) | 243 |
| Bsp143I | GATC | 1 cut(s) | 349 |
| BspACI | CCGC | 4 cut(s) | 311, 335, 649, 721 |
| BspANI | GGCC | 4 cut(s) | 392, 530, 579, 648 |
| BspCNI | CTCAG | 3 cut(s) | 255, 370, 394 |
| BspLI | GGNNCC | 3 cut(s) | 269, 280, 578 |
| BspT107I | GGYRCC | 2 cut(s) | 267, 278 |
| BsrI | ACTGG | 2 cut(s) | 578, 590 |
| BssECI | CCNNGG | 3 cut(s) | 163, 274, 314 |
| BssMI | GATC | 1 cut(s) | 349 |
| BssT1I | CCWWGG | 1 cut(s) | 163 |
| Bst2UI | CCWGG | 1 cut(s) | 276 |
| Bst4CI | ACNGT | 3 cut(s) | 264, 457, 717 |
| Bst6I | CTCTTC | 1 cut(s) | 52 |
| BstC8I | GCNNGC | 1 cut(s) | 311 |
| BstDEI | CTNAG | 4 cut(s) | 191, 242, 357, 381 |
| BstEII | GGTNACC | 1 cut(s) | 490 |
| BstKTI | GATC | 1 cut(s) | 352 |
| BstMBI | GATC | 1 cut(s) | 349 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 276, 648 |
| BstNI | CCWGG | 1 cut(s) | 276 |
| BstNSI | RCATGY | 1 cut(s) | 234 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 497 |
| BstPI | GGTNACC | 1 cut(s) | 490 |
| BstSCI | CCNGG | 3 cut(s) | 274, 313, 548 |
| BstV1I | GCAGC | 2 cut(s) | 197, 349 |
| BstV2I | GAAGAC | 2 cut(s) | 358, 718 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 170 |
| BsuRI | GGCC | 4 cut(s) | 392, 530, 579, 648 |
| BtgZI | GCGATG | 1 cut(s) | 657 |
| BtsI | GCAGTG | 2 cut(s) | 195, 291 |
| BtsIMutI | CAGTG | 4 cut(s) | 195, 269, 291, 462 |
| Cac8I | GCNNGC | 1 cut(s) | 311 |
| Cfr13I | GGNCC | 3 cut(s) | 78, 390, 577 |
| Csp6I | GTAC | 2 cut(s) | 385, 718 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 484, 519 |
| CviAII | CATG | 2 cut(s) | 89, 231 |
| CviQI | GTAC | 2 cut(s) | 385, 718 |
| DdeI | CTNAG | 4 cut(s) | 191, 242, 357, 381 |
| DpnI | GATC | 1 cut(s) | 351 |
| DpnII | GATC | 1 cut(s) | 349 |
| EaeI | YGGCCR | 2 cut(s) | 528, 646 |
| Eam1104I | CTCTTC | 1 cut(s) | 52 |
| EarI | CTCTTC | 1 cut(s) | 52 |
| Ecl136II | GAGCTC | 1 cut(s) | 241 |
| Eco130I | CCWWGG | 1 cut(s) | 163 |
| Eco24I | GRGCYC | 1 cut(s) | 243 |
| Eco47I | GGWCC | 1 cut(s) | 78 |
| Eco53kI | GAGCTC | 1 cut(s) | 241 |
| Eco88I | CYCGRG | 1 cut(s) | 541 |
| Eco91I | GGTNACC | 1 cut(s) | 490 |
| EcoICRI | GAGCTC | 1 cut(s) | 241 |
| EcoO65I | GGTNACC | 1 cut(s) | 490 |
| EcoRII | CCWGG | 1 cut(s) | 274 |
| EcoT14I | CCWWGG | 1 cut(s) | 163 |
| EcoT38I | GRGCYC | 1 cut(s) | 243 |
| ErhI | CCWWGG | 1 cut(s) | 163 |
| FaeI | CATG | 2 cut(s) | 92, 234 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 184, 216, 607, 639 |
| FaqI | GGGAC | 3 cut(s) | 136, 187, 386 |
| FatI | CATG | 2 cut(s) | 88, 230 |
| FauI | CCCGC | 1 cut(s) | 318 |
| Fnu4HI | GCNGC | 3 cut(s) | 186, 338, 649 |
| FriOI | GRGCYC | 1 cut(s) | 243 |
| Fsp4HI | GCNGC | 3 cut(s) | 186, 338, 649 |
| FspBI | CTAG | 1 cut(s) | 482 |
| GluI | GCNGC | 3 cut(s) | 186, 338, 649 |
| GsuI | CTGGAG | 1 cut(s) | 531 |
| HaeIII | GGCC | 4 cut(s) | 392, 530, 579, 648 |
| HapII | CCGG | 3 cut(s) | 314, 393, 550 |
| Hin1II | CATG | 2 cut(s) | 92, 234 |
| HinfI | GANTC | 1 cut(s) | 16 |
| HpaII | CCGG | 3 cut(s) | 314, 393, 550 |
| HphI | GGTGA | 3 cut(s) | 122, 433, 502 |
| Hpy166II | GTNNAC | 1 cut(s) | 453 |
| Hpy188I | TCNGA | 1 cut(s) | 699 |
| Hpy188III | TCNNGA | 2 cut(s) | 543, 567 |
| Hpy8I | GTNNAC | 1 cut(s) | 453 |
| Hpy99I | CGWCG | 1 cut(s) | 503 |
| HpyAV | CCTTC | 2 cut(s) | 106, 411 |
| HpyCH4III | ACNGT | 3 cut(s) | 264, 457, 717 |
| HpyCH4IV | ACGT | 1 cut(s) | 498 |
| HpyCH4V | TGCA | 2 cut(s) | 92, 429 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 276, 648 |
| HpyF3I | CTNAG | 4 cut(s) | 191, 242, 357, 381 |
| HpySE526I | ACGT | 1 cut(s) | 498 |
| Hsp92II | CATG | 2 cut(s) | 92, 234 |
| Kzo9I | GATC | 1 cut(s) | 349 |
| LmnI | GCTCC | 1 cut(s) | 227 |
| Lsp1109I | GCAGC | 2 cut(s) | 197, 349 |
| LweI | GCATC | 2 cut(s) | 103, 416 |
| MaeI | CTAG | 1 cut(s) | 482 |
| MaeII | ACGT | 1 cut(s) | 498 |
| MaeIII | GTNAC | 3 cut(s) | 289, 490, 631 |
| MalI | GATC | 1 cut(s) | 351 |
| MboI | GATC | 1 cut(s) | 349 |
| MboII | GAAGA | 4 cut(s) | 11, 39, 358, 723 |
| MfeI | CAATTG | 2 cut(s) | 557, 682 |
| MhlI | GDGCHC | 1 cut(s) | 243 |
| MlsI | TGGCCA | 1 cut(s) | 530 |
| MluCI | AATT | 5 cut(s) | 24, 557, 595, 653, 682 |
| MluNI | TGGCCA | 1 cut(s) | 530 |
| MnlI | CCTC | 7 cut(s) | 98, 281, 342, 434, 614, 629, 687 |
| Mox20I | TGGCCA | 1 cut(s) | 530 |
| MscI | TGGCCA | 1 cut(s) | 530 |
| MseI | TTAA | 3 cut(s) | 54, 534, 727 |
| MslI | CAYNNNNRTG | 2 cut(s) | 265, 284 |
| Msp20I | TGGCCA | 1 cut(s) | 530 |
| MspI | CCGG | 3 cut(s) | 314, 393, 550 |
| MspR9I | CCNGG | 3 cut(s) | 276, 315, 550 |
| MunI | CAATTG | 2 cut(s) | 557, 682 |
| MvaI | CCWGG | 1 cut(s) | 276 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 276, 648 |
| NciI | CCSGG | 2 cut(s) | 315, 550 |
| NdeII | GATC | 1 cut(s) | 349 |
| NlaIII | CATG | 2 cut(s) | 92, 234 |
| NlaIV | GGNNCC | 3 cut(s) | 269, 280, 578 |
| NmuCI | GTSAC | 3 cut(s) | 289, 490, 631 |
| NspI | RCATGY | 1 cut(s) | 234 |
| OliI | CACNNNNGTG | 2 cut(s) | 265, 284 |
| PaeR7I | CTCGAG | 1 cut(s) | 541 |
| PfeI | GAWTC | 1 cut(s) | 16 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 526 |
| PkrI | GCNGC | 3 cut(s) | 187, 339, 650 |
| PshAI | GACNNNNGTC | 1 cut(s) | 497 |
| Psp124BI | GAGCTC | 1 cut(s) | 243 |
| Psp6I | CCWGG | 1 cut(s) | 274 |
| PspEI | GGTNACC | 1 cut(s) | 490 |
| PspGI | CCWGG | 1 cut(s) | 274 |
| PspN4I | GGNNCC | 3 cut(s) | 269, 280, 578 |
| PspPI | GGNCC | 3 cut(s) | 78, 390, 577 |
| RsaI | GTAC | 2 cut(s) | 386, 719 |
| RsaNI | GTAC | 2 cut(s) | 385, 718 |
| RseI | CAYNNNNRTG | 2 cut(s) | 265, 284 |
| SacI | GAGCTC | 1 cut(s) | 243 |
| SaqAI | TTAA | 3 cut(s) | 54, 534, 727 |
| SatI | GCNGC | 3 cut(s) | 186, 338, 649 |
| Sau3AI | GATC | 1 cut(s) | 349 |
| Sau96I | GGNCC | 3 cut(s) | 78, 390, 577 |
| ScrFI | CCNGG | 3 cut(s) | 276, 315, 550 |
| SduI | GDGCHC | 1 cut(s) | 243 |
| SetI | ASST | 9 cut(s) | 98, 109, 190, 243, 248, 452, 483, 501, 606 |
| SfaNI | GCATC | 2 cut(s) | 103, 416 |
| Sfr274I | CTCGAG | 1 cut(s) | 541 |
| SinI | GGWCC | 1 cut(s) | 78 |
| SlaI | CTCGAG | 1 cut(s) | 541 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 265, 284 |
| SmlI | CTYRAG | 1 cut(s) | 541 |
| SmoI | CTYRAG | 1 cut(s) | 541 |
| Sse9I | AATT | 5 cut(s) | 24, 557, 595, 653, 682 |
| SsiI | CCGC | 4 cut(s) | 311, 335, 649, 721 |
| SspMI | CTAG | 1 cut(s) | 482 |
| SstI | GAGCTC | 1 cut(s) | 243 |
| StyD4I | CCNGG | 3 cut(s) | 274, 313, 548 |
| StyI | CCWWGG | 1 cut(s) | 163 |
| TaaI | ACNGT | 3 cut(s) | 264, 457, 717 |
| TaiI | ACGT | 1 cut(s) | 501 |
| TaqI | TCGA | 1 cut(s) | 542 |
| TasI | AATT | 5 cut(s) | 24, 557, 595, 653, 682 |
| TatI | WGTACW | 1 cut(s) | 384 |
| TauI | GCSGC | 1 cut(s) | 651 |
| TfiI | GAWTC | 1 cut(s) | 16 |
| Tru1I | TTAA | 3 cut(s) | 54, 534, 727 |
| Tru9I | TTAA | 3 cut(s) | 54, 534, 727 |
| TscAI | CASTG | 4 cut(s) | 202, 269, 298, 462 |
| TseFI | GTSAC | 3 cut(s) | 289, 490, 631 |
| TseI | GCWGC | 2 cut(s) | 185, 337 |
| Tsp45I | GTSAC | 3 cut(s) | 289, 490, 631 |
| TspRI | CASTG | 4 cut(s) | 202, 269, 298, 462 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 526 |
| VpaK11BI | GGWCC | 1 cut(s) | 78 |
| XceI | RCATGY | 1 cut(s) | 234 |
| XhoI | CTCGAG | 1 cut(s) | 541 |
| XspI | CTAG | 1 cut(s) | 482 |