MD14G1096700.v1.1

iron ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000132
Physical Location & Seq
Forward (+)
14681301 .. 14683101
1801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1096700.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGACAAGATCCTTGATGAAATCATCAGCGACCATAGAGTGAAAAGGAATGAGGAATCAGCTGCTCGAGATGACCATCAGGAAGATATAGTTGATGTGCTACTGCGGCTCCAGGAGTCTGGTGAGCTCCAGTTTGATCTCACAACCACCCAAATCAAAGCTGTCACCCTGGACTTGTTTTCTGCTGGAAGTGAGAGTTCAGCAACTGCGATAGAATGGGCAATGGCTGAACTTCTGAGAAGTCCAATAGCAATGAAACAAGCACAGGCCGAAGTACGCCAACTTGTCGGAGGAAAGGGGAAGATTGAAGAGGCAGACATTAAGAAACTCGACTACCTGAAGTTGGTCATAAGAGAAACTCTACGATTACACCCTCCTCCTTTCATCCCAAGACAAGCAAGCCAAAAATGCACTATTGGCGGGTATGATATACCAACTGATGCGACAGTCTTCATCAATGCTTGGGGAATTGGGAGAGACTCCAAGTATTGGGAGGATGCTGATTGTTTTCTGCCGGAGAGATTCCAAGGCTCTTCCGTTGATTTTCGAGGGTCCAACTTCGAATTGCTTCCATTTGGGGGTGGCAGGAGAATCTGTCCAGGCATATCATTCGCAACCGCTGGAATTGAACTTGCGCTTTCTCATCTGCTCTACCACTTTAACTGGAAACTTCCCAATGCAAATGGCAGTGGCAATGAGACAAGGTTATCAGAAGAGCTTGACATGACTGAATGTAACGGATTGAGTATTAGGAAGAAGAATAATTTGTATCTCATCGCCATTCCTTTCATTTCGTCATCGGAAATGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

271

Amino Acids

30.19

Weight (kDa)

5.09

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 228 4e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 489
AciI CCGC 3 cut(s) 106, 420, 618
AclWI GGATC 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 343, 489, 578
AgsI TTSAA 2 cut(s) 308, 629
AjnI CCWGG 3 cut(s) 111, 168, 598
AluBI AGCT 4 cut(s) 62, 127, 161, 718
AluI AGCT 4 cut(s) 62, 127, 161, 718
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 471, 692
AlwI GGATC 1 cut(s) 4
AlwNI CAGNNNCTG 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 1 cut(s) 552
AsuHPI GGTGA 2 cut(s) 134, 157
AsuII TTCGAA 1 cut(s) 561
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 552
BanII GRGCYC 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 442
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 49
BccI CCATC 1 cut(s) 84
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BciT130I CCWGG 3 cut(s) 113, 170, 600
BcoDI GTCTC 2 cut(s) 471, 692
BisI GCNGC 2 cut(s) 63, 107
BlsI GCNGC 2 cut(s) 64, 108
Bme1390I CCNGG 3 cut(s) 113, 170, 600
Bme18I GGWCC 1 cut(s) 552
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 110, 553
BmrFI CCNGG 3 cut(s) 113, 170, 600
BmsI GCATC 2 cut(s) 430, 487
BpiI GAAGAC 1 cut(s) 442
BpmI CTGGAG 2 cut(s) 95, 113
Bpu14I TTCGAA 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 168, 526
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 580, 610
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 489, 578
Bse1I ACTGG 2 cut(s) 130, 668
Bse3DI GCAATG 3 cut(s) 228, 258, 700
Bse8I GATNNNNATC 1 cut(s) 75
BseBI CCWGG 3 cut(s) 113, 170, 600
BseDI CCNNGG 2 cut(s) 168, 526
BseGI GGATG 2 cut(s) 384, 502
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 3 cut(s) 343, 489, 578
BseMI GCAATG 3 cut(s) 228, 258, 700
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 2 cut(s) 130, 668
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 49
BshFI GGCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 343, 489, 578
BsmAI GTCTC 2 cut(s) 471, 692
BsnI GGCC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 66
Bsp119I TTCGAA 1 cut(s) 561
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 2 cut(s) 9, 136
BspACI CCGC 3 cut(s) 106, 420, 618
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 110, 553
BspPI GGATC 1 cut(s) 4
BspQI GCTCTTC 2 cut(s) 538, 708
BspT104I TTCGAA 1 cut(s) 561
BsrDI GCAATG 3 cut(s) 228, 258, 700
BsrI ACTGG 2 cut(s) 130, 668
BssECI CCNNGG 2 cut(s) 168, 526
BssMI GATC 2 cut(s) 9, 136
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 3 cut(s) 113, 170, 600
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 3 cut(s) 303, 538, 708
BstBI TTCGAA 1 cut(s) 561
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 384, 502
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 2 cut(s) 12, 139
BstMAI GTCTC 2 cut(s) 471, 692
BstMBI GATC 2 cut(s) 9, 136
BstMWI GCNNNNNNNGC 3 cut(s) 106, 408, 417
BstNI CCWGG 3 cut(s) 113, 170, 600
BstSCI CCNGG 3 cut(s) 111, 168, 598
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 269
BtgZI GCGATG 1 cut(s) 760
BtsCI GGATG 2 cut(s) 384, 502
BtsI GCAGTG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 694
Cac8I GCNNGC 1 cut(s) 400
CaiI CAGNNNCTG 1 cut(s) 206
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 1 cut(s) 552
Csp6I GTAC 1 cut(s) 275
CviAII CATG 1 cut(s) 724
CviJI RGCY 9 cut(s) 62, 109, 127, 161, 227, 269, 402, 531, 718
CviKI_1 RGCY 9 cut(s) 62, 109, 127, 161, 227, 269, 402, 531, 718
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 1 cut(s) 236
DpnI GATC 2 cut(s) 11, 138
DpnII GATC 2 cut(s) 9, 136
Eam1104I CTCTTC 3 cut(s) 303, 538, 708
EarI CTCTTC 3 cut(s) 303, 538, 708
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 552
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 359
Eco88I CYCGRG 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 127
EcoRII CCWGG 3 cut(s) 111, 168, 598
EcoT14I CCWWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 526
FaeI CATG 1 cut(s) 727
FaiI YATR 7 cut(s) 36, 89, 350, 426, 431, 605, 725
FatI CATG 1 cut(s) 723
FauI CCCGC 1 cut(s) 413
Fnu4HI GCNGC 2 cut(s) 63, 107
FokI GGATG 2 cut(s) 371, 509
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 2 cut(s) 63, 107
GlaI GCGC 1 cut(s) 636
GluI GCNGC 2 cut(s) 63, 107
GsuI CTGGAG 2 cut(s) 95, 113
HaeIII GGCC 1 cut(s) 269
HapII CCGG 1 cut(s) 515
HhaI GCGC 1 cut(s) 637
Hin1II CATG 1 cut(s) 727
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HinfI GANTC 5 cut(s) 56, 116, 479, 522, 591
HpaII CCGG 1 cut(s) 515
HphI GGTGA 2 cut(s) 134, 157
Hpy188I TCNGA 4 cut(s) 237, 290, 712, 802
Hpy188III TCNNGA 2 cut(s) 68, 80
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4V TGCA 2 cut(s) 411, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 408, 417
HpyF3I CTNAG 1 cut(s) 236
Hsp92II CATG 1 cut(s) 727
HspAI GCGC 1 cut(s) 635
Kzo9I GATC 2 cut(s) 9, 136
LguI GCTCTTC 2 cut(s) 538, 708
LmnI GCTCC 2 cut(s) 114, 132
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 2 cut(s) 430, 487
MaeIII GTNAC 2 cut(s) 163, 734
MalI GATC 2 cut(s) 11, 138
MboI GATC 2 cut(s) 9, 136
MboII GAAGA 8 cut(s) 95, 313, 320, 442, 525, 725, 766, 769
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 5 cut(s) 468, 563, 624, 763, 808
MlyI GAGTC 2 cut(s) 125, 473
MmeI TCCRAC 2 cut(s) 268, 579
MnlI CCTC 7 cut(s) 46, 284, 304, 384, 387, 487, 542
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 2 cut(s) 321, 660
MspA1I CMGCKG 2 cut(s) 62, 620
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 3 cut(s) 113, 170, 600
MvaI CCWGG 3 cut(s) 113, 170, 600
MwoI GCNNNNNNNGC 3 cut(s) 106, 408, 417
NdeII GATC 2 cut(s) 9, 136
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 2 cut(s) 110, 553
NmuCI GTSAC 1 cut(s) 163
NspV TTCGAA 1 cut(s) 561
PaeR7I CTCGAG 1 cut(s) 66
PciSI GCTCTTC 2 cut(s) 538, 708
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 3 cut(s) 56, 522, 591
PflMI CCANNNNNTGG 1 cut(s) 489
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 2 cut(s) 64, 108
PleI GAGTC 2 cut(s) 124, 473
PpsI GAGTC 2 cut(s) 124, 473
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 3 cut(s) 111, 168, 598
PspGI CCWGG 3 cut(s) 111, 168, 598
PspN4I GGNNCC 2 cut(s) 110, 553
PspPI GGNCC 1 cut(s) 552
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 1 cut(s) 9
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SacI GAGCTC 1 cut(s) 129
SapI GCTCTTC 2 cut(s) 538, 708
SaqAI TTAA 2 cut(s) 321, 660
SatI GCNGC 2 cut(s) 63, 107
Sau3AI GATC 2 cut(s) 9, 136
Sau96I GGNCC 1 cut(s) 552
SchI GAGTC 2 cut(s) 125, 473
ScrFI CCNGG 3 cut(s) 113, 170, 600
SduI GDGCHC 1 cut(s) 129
SetI ASST 6 cut(s) 64, 129, 163, 339, 707, 720
SfaNI GCATC 2 cut(s) 430, 487
Sfr274I CTCGAG 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 561
SinI GGWCC 1 cut(s) 552
SlaI CTCGAG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 5 cut(s) 468, 563, 624, 763, 808
SsiI CCGC 3 cut(s) 106, 420, 618
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 111, 168, 598
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 1 cut(s) 448
TaqI TCGA 4 cut(s) 67, 330, 547, 561
TasI AATT 5 cut(s) 468, 563, 624, 763, 808
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 3 cut(s) 56, 522, 591
Tru1I TTAA 2 cut(s) 321, 660
Tru9I TTAA 2 cut(s) 321, 660
TscAI CASTG 1 cut(s) 694
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 33, 269, 373, 442, 778
TspGWI ACGGA 2 cut(s) 526, 753
TspRI CASTG 1 cut(s) 694
Van91I CCANNNNNTGG 1 cut(s) 489
VpaK11BI GGWCC 1 cut(s) 552
XhoI CTCGAG 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.