MD00G1138500.v1.1

Premnaspirodiene oxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000132
Physical Location & Seq
Reverse (-)
30021455 .. 30023352
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1138500.v1.1.491

Sequence Viewer

Length: 1680 bp
ATGCCTTGGTTTGCAAGTAAAATACCCCAAATTCGTATGATGAACTTGATCACAGCTTATCTGGATCAACTTATGTTTCTAATTGTCGAAACGCCCTATATGTCTTTTTTCACTTCTCTCCTTCTCTGCATCCTGGTGTTTTTTTGGAAGCGATCAAGATCCGGAGGCCGAAAGTCACCCCCAGGGCCGTGGAAGCTGCCTGTTATTGGAAACTTGCATCAGGTGGTCGGTCCGCTGCCACACCACACACTGAGAGCCTTAGCCAGGAAACATGGACCCATCATGCACCTTAAACTGGGTCAAATAGAGGCCGTGATTATTTCATCTGCCAAAGCTGCCCGGGAAGTGTTGAAGACACACGAGCTTGCATTTGCACAGAGGCCTGTGGTTTTGGCAGCAGAGGTTATGTCTTTTGGTCACGGAAGTATTGTCTTTGCTCCTTATGGAGATTTATGGAGATCGCTGAGAAAGATTTCTATTTTCGAGCTACTGAGCGTGAAACGTGTGCAGTCTTTGAGATCCGTAAGAGAAGAAGAGGTGTGGAATCTTGTTGAGTCCATTGGCTCTACGTCTCACAAGGGACTTGCCCTCAATTTTAGTGACAGGTGCGGCAGCTTCACAAATGACGTAGTGTCAAAGGCAACATTTGGGAAAAAATGCAAAGACCAAAAGGAGTTCCTTTCGCTGCTAACTGAATCAACCAAAGTCGCTGGTGGGTTCGATATTCCTGATTTGTTTCCTTCACTCAGTTTTCTTGGGTACGTTACTGGGTCAATCCCTGCTATGAAAGACATACATAGCAAGCTCGGTAGGATTCTCGAAAATATCATCAACGATCATAAGATCAAAAGATCCAAAAAGGACTTGATGATCAGTAATACTAGGACTGCTACAGCAGGCAATGGCAAAGCAGAGGAGGAGGAGGAGGGGGAGGAGGGAAATTTTGTTGACGTATTTCTAAAACTTAAGGAGTCCAGTAAGGCGGAGTTCAGCTTGACAACCAATCAAATCAAAGATGTCATTCTGGTGAGTAATGAAATCTTCTCTGCAGGAAGCGAGACTTCAGCTACTACCTTAGAATGGGTAATGTCAGAGTTGATGAGAAACCCAAGAGTCATGAAGAGGGCTCAAGCTGAAGTGAGACAGTTAGTCCTCCAATTTGAAGGAAAGAAAAGCAAAGTTATTGAAGAAGAAGATGTTCAAAAAATGGACTACCTGAAATCGGTGGTGAAAGAAACTCTGAGGTTACACTCTCCAGCCCCTTTGCTCCCAAGAGAAGCAAGGGACACGGTTCAAATTGGCGGGTTCGAATTACCAGTTAAATCAAAAGTAATTATTAATCTATGGGCAATGGGAAGAGACCCAGAGATATGGGGGGCAGATGCTGAGTGTTTTAAGCCGGAGAGGTTTCAGGGTTCTTCTGTTGACTTTAAGGGTTTTGACTTTGAGTTCACCCCGTTTGGGGCCGGCAGAAGAATGTGTCCAGGCATGTTATTTGGTGTTACCATGGTTGAACTTGCTCTTGCTGAATTGCTCTACCGCTTCGATTGGAAACTGGCGAATGGGATGAATCCAGACGAACTTGACATGACGGAGACCTTTGGAGTGACCTGTAAGAGAAAGAATGCGTTGTACCTAATTGCCACCCCACATTTTACTTCAGTCAATGAGTCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

560

Amino Acids

62.82

Weight (kDa)

8.78

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 60 - 543 1.1e-88 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 5 cut(s) 233, 609, 983, 1302, 1540
AclWI GGATC 4 cut(s) 72, 153, 513, 846
AcsI RAATTY 2 cut(s) 30, 940
AcuI CTGAAG 3 cut(s) 1047, 1155, 1644
AfaI GTAC 2 cut(s) 761, 1634
AfiI CCNNNNNNNGG 7 cut(s) 206, 264, 295, 1080, 1222, 1461, 1462
AflII CTTAAG 1 cut(s) 965
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 6 cut(s) 352, 1163, 1187, 1202, 1295, 1514
AhdI GACNNNNNGTC 2 cut(s) 631, 1148
AjnI CCWGG 4 cut(s) 132, 181, 263, 1483
Alw26I GTCTC 5 cut(s) 576, 1052, 1135, 1353, 1589
AlwI GGATC 4 cut(s) 72, 153, 513, 846
AlwNI CAGNNNCTG 1 cut(s) 1385
Ama87I CYCGRG 1 cut(s) 339
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 5 cut(s) 166, 185, 309, 380, 1464
ApeKI GCWGC 6 cut(s) 196, 235, 335, 395, 612, 685
ApoI RAATTY 2 cut(s) 30, 940
AseI ATTAAT 1 cut(s) 1338
Asp700I GAANNNNTTC 2 cut(s) 472, 1197
AspS9I GGNCC 4 cut(s) 185, 230, 275, 1464
AsuC2I CCSGG 2 cut(s) 340, 341
AsuHPI GGTGA 4 cut(s) 168, 1039, 1240, 1444
AsuII TTCGAA 1 cut(s) 1308
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 2 cut(s) 230, 275
BanII GRGCYC 1 cut(s) 1129
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 359
BbvI GCAGC 6 cut(s) 183, 222, 322, 407, 624, 672
BccI CCATC 1 cut(s) 287
BceAI ACGGC 2 cut(s) 172, 296
BciT130I CCWGG 4 cut(s) 134, 183, 265, 1485
BclI TGATCA 2 cut(s) 48, 870
BcnI CCSGG 2 cut(s) 340, 341
BcoDI GTCTC 5 cut(s) 576, 1052, 1135, 1353, 1589
BfaI CTAG 1 cut(s) 882
BfmI CTRYAG 2 cut(s) 891, 1047
BfrI CTTAAG 1 cut(s) 965
BisI GCNGC 7 cut(s) 197, 236, 336, 396, 610, 613, 686
BlsI GCNGC 7 cut(s) 198, 237, 337, 397, 611, 614, 687
Bme1390I CCNGG 6 cut(s) 134, 183, 265, 340, 341, 1485
Bme18I GGWCC 2 cut(s) 230, 275
BmeRI GACNNNNNGTC 2 cut(s) 631, 1148
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 4 cut(s) 185, 230, 275, 1464
BmiI GGNNCC 2 cut(s) 277, 1465
BmrFI CCNGG 6 cut(s) 134, 183, 265, 340, 341, 1485
BmrI ACTGGG 2 cut(s) 305, 777
BmsI GCATC 3 cut(s) 138, 226, 1372
BmuI ACTGGG 2 cut(s) 305, 777
BpiI GAAGAC 1 cut(s) 359
BpmI CTGGAG 1 cut(s) 1239
Bpu10I CCTNAGC 1 cut(s) 259
Bpu14I TTCGAA 1 cut(s) 1308
BpuEI CTTGAG 1 cut(s) 1113
BpuMI CCSGG 2 cut(s) 340, 341
BsaBI GATNNNNATC 1 cut(s) 157
BsaI GGTCTC 2 cut(s) 1353, 1589
BsaJI CCNNGG 6 cut(s) 5, 181, 182, 188, 339, 1506
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 7 cut(s) 206, 264, 295, 1080, 1222, 1461, 1462
Bse118I RCCGGY 1 cut(s) 1466
Bse1I ACTGG 5 cut(s) 300, 772, 975, 1316, 1560
Bse3DI GCAATG 2 cut(s) 907, 1356
Bse8I GATNNNNATC 1 cut(s) 157
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 4 cut(s) 134, 183, 265, 1485
BseDI CCNNGG 6 cut(s) 5, 181, 182, 188, 339, 1506
BseGI GGATG 2 cut(s) 129, 1572
BseJI GATNNNNATC 1 cut(s) 157
BseLI CCNNNNNNNGG 7 cut(s) 206, 264, 295, 1080, 1222, 1461, 1462
BseMI GCAATG 2 cut(s) 907, 1356
BseMII CTCAG 6 cut(s) 242, 455, 482, 760, 1232, 1377
BseNI ACTGG 5 cut(s) 300, 772, 975, 1316, 1560
BseRI GAGGAG 5 cut(s) 929, 932, 935, 938, 947
BseXI GCAGC 6 cut(s) 183, 222, 322, 407, 624, 672
BsgI GTGCAG 1 cut(s) 527
BshFI GGCC 5 cut(s) 168, 187, 311, 382, 1466
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 4 cut(s) 162, 340, 1400, 1467
BslFI GGGAC 2 cut(s) 594, 1298
BslI CCNNNNNNNGG 7 cut(s) 206, 264, 295, 1080, 1222, 1461, 1462
BsmAI GTCTC 5 cut(s) 576, 1052, 1135, 1353, 1589
BsmBI CGTCTC 1 cut(s) 576
BsmFI GGGAC 2 cut(s) 594, 1298
BsmI GAATGC 1 cut(s) 1630
BsnI GGCC 5 cut(s) 168, 187, 311, 382, 1466
Bso31I GGTCTC 2 cut(s) 1353, 1589
BsoBI CYCGRG 1 cut(s) 339
Bsp119I TTCGAA 1 cut(s) 1308
Bsp1286I GDGCHC 1 cut(s) 1129
Bsp13I TCCGGA 1 cut(s) 161
Bsp19I CCATGG 1 cut(s) 1506
BspACI CCGC 5 cut(s) 233, 609, 983, 1302, 1540
BspANI GGCC 5 cut(s) 168, 187, 311, 382, 1466
BspCNI CTCAG 6 cut(s) 243, 456, 483, 759, 1233, 1378
BspEI TCCGGA 1 cut(s) 161
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 2 cut(s) 277, 1465
BspMAI CTGCAG 1 cut(s) 1051
BspPI GGATC 4 cut(s) 72, 153, 513, 846
BspT104I TTCGAA 1 cut(s) 1308
BspTI CTTAAG 1 cut(s) 965
BspTNI GGTCTC 2 cut(s) 1353, 1589
BsrDI GCAATG 2 cut(s) 907, 1356
BsrFI RCCGGY 1 cut(s) 1466
BsrI ACTGG 5 cut(s) 300, 772, 975, 1316, 1560
BssAI RCCGGY 1 cut(s) 1466
BssECI CCNNGG 6 cut(s) 5, 181, 182, 188, 339, 1506
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 2 cut(s) 5, 1506
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 4 cut(s) 134, 183, 265, 1485
Bst4CI ACNGT 2 cut(s) 1146, 1291
Bst6I CTCTTC 3 cut(s) 528, 1115, 1351
BstAFI CTTAAG 1 cut(s) 965
BstBI TTCGAA 1 cut(s) 1308
BstC8I GCNNGC 4 cut(s) 366, 803, 898, 1468
BstDEI CTNAG 8 cut(s) 251, 259, 464, 491, 746, 1075, 1241, 1386
BstDSI CCRYGG 2 cut(s) 188, 1506
BstENI CCTNNNNNAGG 1 cut(s) 262
BstF5I GGATG 2 cut(s) 129, 1572
BstMAI GTCTC 5 cut(s) 576, 1052, 1135, 1353, 1589
BstMWI GCNNNNNNNGC 2 cut(s) 193, 335
BstNI CCWGG 4 cut(s) 134, 183, 265, 1485
BstNSI RCATGY 1 cut(s) 1492
BstSCI CCNGG 6 cut(s) 132, 181, 263, 338, 339, 1483
BstSFI CTRYAG 2 cut(s) 891, 1047
BstV1I GCAGC 6 cut(s) 183, 222, 322, 407, 624, 672
BstV2I GAAGAC 1 cut(s) 359
BstX2I RGATCY 3 cut(s) 158, 518, 851
BstXI CCANNNNNNTGG 2 cut(s) 189, 1371
BstYI RGATCY 3 cut(s) 158, 518, 851
BsuRI GGCC 5 cut(s) 168, 187, 311, 382, 1466
BtgI CCRYGG 2 cut(s) 188, 1506
BtsCI GGATG 2 cut(s) 129, 1572
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 4 cut(s) 366, 803, 898, 1468
CaiI CAGNNNCTG 1 cut(s) 1385
CciI TCATGA 1 cut(s) 1116
Cfr10I RCCGGY 1 cut(s) 1466
Cfr13I GGNCC 4 cut(s) 185, 230, 275, 1464
Cfr9I CCCGGG 1 cut(s) 339
CpoI CGGWCCG 1 cut(s) 230
Csp6I GTAC 2 cut(s) 760, 1633
CspI CGGWCCG 1 cut(s) 230
CviAII CATG 6 cut(s) 272, 283, 1117, 1489, 1507, 1588
CviQI GTAC 2 cut(s) 760, 1633
DdeI CTNAG 8 cut(s) 251, 259, 464, 491, 746, 1075, 1241, 1386
DriI GACNNNNNGTC 2 cut(s) 631, 1148
Eam1104I CTCTTC 3 cut(s) 528, 1115, 1351
Eam1105I GACNNNNNGTC 2 cut(s) 631, 1148
EarI CTCTTC 3 cut(s) 528, 1115, 1351
EciI GGCGGA 1 cut(s) 998
Eco130I CCWWGG 2 cut(s) 5, 1506
Eco147I AGGCCT 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 1129
Eco31I GGTCTC 2 cut(s) 1353, 1589
Eco47I GGWCC 2 cut(s) 230, 275
Eco57I CTGAAG 3 cut(s) 1047, 1155, 1644
Eco88I CYCGRG 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 262
EcoRII CCWGG 4 cut(s) 132, 181, 263, 1483
EcoT14I CCWWGG 2 cut(s) 5, 1506
EcoT38I GRGCYC 1 cut(s) 1129
ErhI CCWWGG 2 cut(s) 5, 1506
Esp3I CGTCTC 1 cut(s) 576
FaeI CATG 6 cut(s) 275, 286, 1120, 1492, 1510, 1591
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 2 cut(s) 594, 1298
FatI CATG 6 cut(s) 271, 282, 1116, 1488, 1506, 1587
FauI CCCGC 1 cut(s) 1295
FbaI TGATCA 2 cut(s) 48, 870
Fnu4HI GCNGC 7 cut(s) 197, 236, 336, 396, 610, 613, 686
FokI GGATG 2 cut(s) 116, 1579
FriOI GRGCYC 1 cut(s) 1129
Fsp4HI GCNGC 7 cut(s) 197, 236, 336, 396, 610, 613, 686
FspBI CTAG 1 cut(s) 882
GluI GCNGC 7 cut(s) 197, 236, 336, 396, 610, 613, 686
GsuI CTGGAG 1 cut(s) 1239
HaeIII GGCC 5 cut(s) 168, 187, 311, 382, 1466
HapII CCGG 4 cut(s) 162, 340, 1400, 1467
Hin1II CATG 6 cut(s) 275, 286, 1120, 1492, 1510, 1591
HincII GTYRAC 2 cut(s) 949, 1426
HindII GTYRAC 2 cut(s) 949, 1426
HinfI GANTC 8 cut(s) 544, 554, 695, 814, 971, 1113, 1570, 1670
HpaII CCGG 4 cut(s) 162, 340, 1400, 1467
HphI GGTGA 4 cut(s) 168, 1039, 1240, 1444
Hpy166II GTNNAC 3 cut(s) 949, 1426, 1452
Hpy188I TCNGA 3 cut(s) 1093, 1242, 1675
Hpy188III TCNNGA 7 cut(s) 62, 156, 162, 728, 818, 1117, 1574
Hpy8I GTNNAC 3 cut(s) 949, 1426, 1452
HpyAV CCTTC 3 cut(s) 131, 750, 1157
HpyCH4III ACNGT 2 cut(s) 1146, 1291
HpyCH4IV ACGT 5 cut(s) 502, 569, 627, 762, 951
HpyCH4V TGCA 9 cut(s) 14, 129, 217, 286, 368, 374, 508, 660, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 335
HpyF3I CTNAG 8 cut(s) 251, 259, 464, 491, 746, 1075, 1241, 1386
HpySE526I ACGT 5 cut(s) 502, 569, 627, 762, 951
Hsp92II CATG 6 cut(s) 275, 286, 1120, 1492, 1510, 1591
Kpn2I TCCGGA 1 cut(s) 161
KroI GCCGGC 1 cut(s) 1466
KroNI GCCGGC 1 cut(s) 1468
Ksp22I TGATCA 2 cut(s) 48, 870
LmnI GCTCC 2 cut(s) 442, 1272
Lsp1109I GCAGC 6 cut(s) 183, 222, 322, 407, 624, 672
LweI GCATC 3 cut(s) 138, 226, 1372
MaeI CTAG 1 cut(s) 882
MaeII ACGT 5 cut(s) 502, 569, 627, 762, 951
MaeIII GTNAC 7 cut(s) 174, 416, 599, 763, 1245, 1501, 1606
MflI RGATCY 3 cut(s) 158, 518, 851
MhlI GDGCHC 1 cut(s) 1129
MlyI GAGTC 4 cut(s) 563, 980, 1122, 1679
MroI TCCGGA 1 cut(s) 161
MroNI GCCGGC 1 cut(s) 1466
MroXI GAANNNNTTC 2 cut(s) 472, 1197
MseI TTAA 6 cut(s) 291, 966, 1320, 1338, 1395, 1431
MslI CAYNNNNRTG 2 cut(s) 134, 1025
MspA1I CMGCKG 1 cut(s) 235
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 4 cut(s) 162, 340, 1400, 1467
MspR9I CCNGG 6 cut(s) 134, 183, 265, 340, 341, 1485
Mva1269I GAATGC 1 cut(s) 1630
MvaI CCWGG 4 cut(s) 134, 183, 265, 1485
MwoI GCNNNNNNNGC 2 cut(s) 193, 335
NaeI GCCGGC 1 cut(s) 1468
NciI CCSGG 2 cut(s) 340, 341
NcoI CCATGG 1 cut(s) 1506
NgoMIV GCCGGC 1 cut(s) 1466
NlaIII CATG 6 cut(s) 275, 286, 1120, 1492, 1510, 1591
NlaIV GGNNCC 2 cut(s) 277, 1465
NmuCI GTSAC 4 cut(s) 174, 416, 599, 1606
NspI RCATGY 1 cut(s) 1492
NspV TTCGAA 1 cut(s) 1308
PagI TCATGA 1 cut(s) 1116
PasI CCCWGGG 1 cut(s) 182
PceI AGGCCT 1 cut(s) 382
PctI GAATGC 1 cut(s) 1630
PdiI GCCGGC 1 cut(s) 1468
PdmI GAANNNNTTC 2 cut(s) 472, 1197
PfeI GAWTC 4 cut(s) 544, 695, 814, 1570
PkrI GCNGC 7 cut(s) 198, 237, 337, 397, 611, 614, 687
PleI GAGTC 4 cut(s) 562, 979, 1121, 1678
PpsI GAGTC 4 cut(s) 562, 979, 1121, 1678
PshBI ATTAAT 1 cut(s) 1338
Psp6I CCWGG 4 cut(s) 132, 181, 263, 1483
PspGI CCWGG 4 cut(s) 132, 181, 263, 1483
PspN4I GGNNCC 2 cut(s) 277, 1465
PspPI GGNCC 4 cut(s) 185, 230, 275, 1464
PstI CTGCAG 1 cut(s) 1051
PstNI CAGNNNCTG 1 cut(s) 1385
PsuI RGATCY 3 cut(s) 158, 518, 851
RsaI GTAC 2 cut(s) 761, 1634
RsaNI GTAC 2 cut(s) 760, 1633
RseI CAYNNNNRTG 2 cut(s) 134, 1025
Rsr2I CGGWCCG 1 cut(s) 230
RsrII CGGWCCG 1 cut(s) 230
SaqAI TTAA 6 cut(s) 291, 966, 1320, 1338, 1395, 1431
SatI GCNGC 7 cut(s) 197, 236, 336, 396, 610, 613, 686
Sau96I GGNCC 4 cut(s) 185, 230, 275, 1464
SchI GAGTC 4 cut(s) 563, 980, 1122, 1679
ScrFI CCNGG 6 cut(s) 134, 183, 265, 340, 341, 1485
SduI GDGCHC 1 cut(s) 1129
SfaNI GCATC 3 cut(s) 138, 226, 1372
SfcI CTRYAG 2 cut(s) 891, 1047
SfuI TTCGAA 1 cut(s) 1308
SinI GGWCC 2 cut(s) 230, 275
SmaI CCCGGG 1 cut(s) 341
SmiMI CAYNNNNRTG 2 cut(s) 134, 1025
SmlI CTYRAG 2 cut(s) 965, 1128
SmoI CTYRAG 2 cut(s) 965, 1128
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 5 cut(s) 233, 609, 983, 1302, 1540
SspMI CTAG 1 cut(s) 882
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 6 cut(s) 132, 181, 263, 338, 339, 1483
StyI CCWWGG 2 cut(s) 5, 1506
TaaI ACNGT 2 cut(s) 1146, 1291
TaiI ACGT 5 cut(s) 505, 572, 630, 765, 954
TaqI TCGA 6 cut(s) 87, 483, 720, 819, 1308, 1545
TaqII GACCGA 1 cut(s) 218
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 4 cut(s) 544, 695, 814, 1570
Tru1I TTAA 6 cut(s) 291, 966, 1320, 1338, 1395, 1431
Tru9I TTAA 6 cut(s) 291, 966, 1320, 1338, 1395, 1431
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 4 cut(s) 174, 416, 599, 1606
TseI GCWGC 6 cut(s) 196, 235, 335, 395, 612, 685
Tsp45I GTSAC 4 cut(s) 174, 416, 599, 1606
TspDTI ATGAA 6 cut(s) 56, 312, 800, 1050, 1133, 1583
TspGWI ACGGA 3 cut(s) 435, 511, 1607
TspMI CCCGGG 1 cut(s) 339
TspRI CASTG 1 cut(s) 255
Vha464I CTTAAG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 230, 275
VspI ATTAAT 1 cut(s) 1338
XagI CCTNNNNNAGG 1 cut(s) 262
XapI RAATTY 2 cut(s) 30, 940
XceI RCATGY 1 cut(s) 1492
XmaI CCCGGG 1 cut(s) 339
XmnI GAANNNNTTC 2 cut(s) 472, 1197
XspI CTAG 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.