MD00G1138600.v1.1

Premnaspirodiene oxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000132
Physical Location & Seq
Reverse (-)
30033617 .. 30035867
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1138600.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGATGAAAGTGATCACAGCTTATCTGAATCAAATTATGTTTCTAATTGTCGAAACGCCCTATTTTTCTTTTTTCACTTCTCTCCTTGTCTGCATCCTGGTGATTTTTTGGAAGCGATCCAGAGCCAGAGGCCTGAAGTCACCCCCAGGGCCATGGAAGCTGCCGATTATTGGAAACTTGCATCAAATGGTCGGTCCACTGCCACACCACACATTGAGAGACTTGGCCAAGAAACATGGACCCATCATGCACCTTAAACTGGGTCAATTAGAGGCCGTGATTATTTCATCTGCCAAAGCTGCCCGGGAGGTGTTGAAGACACATGAGCTTGCATTTGCGCAGAGGCCTATTGTTTTGGCGGCAGAGGTTATGTCTTTTGGTCAAGGAGGTATTATCTTTGCTCCTTATGGAGATTTATGGAGATCGCTGAGAAAGATTTGCATTTTCGAGCTACTAAGCGCCAAACGTGTGCAGTCTTTCAGATCCGTAAGAGAAGAAGAGGTACGGAATCTTATGGAGTCCATTGGCTCAATGTCTCACAAGGGACTTGCCATCAATTTTAGTGACAGGTGCTGCTGCTTCGCAAATGACGTAGTGTCAAACGCAGCATTTGGGAAAAATTGCAAAGACCAAAAGGAGTTCCTTTCGCTACTAGACGAATCAGCCAAACTTGCTAGCGGGTTTGATCTTCCTGATTTGTTTCCTTCACTCAGTTTTCTTGGATTCATTACTGGGTCGATCCCTGCTATGAAAGACGTACAAAGCAAGCTCGGTACAATTCTCGAAAATATCATCAACGATCATAAGATCAAACGATCCAAAAAGGACTTGAAGATCAGTAGTGCTAATACTACTACAGCAGGCAATGACAAAGCGGAGGAGGAGGAGGAGGAGGAGGAGGAAAATTTTGTTGGCGTACTTCTAAAACTGAAAGAGTCCAATAAGGCGGAGTTCAACTTCACAACCAATCAGATCAAAGATATCATTCTGGACATCTTCGGTGCAGGAAGCGAGACTTCAGCTACTACCTTAGAATGGGCAATGTCAGAGTTGATGAGAAACCCAAGAGTCATGAAGAGGGCTCAATCTGAAGTGAGACAGTTAGTCCTCCAATCTGACGGAAAGAAAAACAAAGTTATTGAAGAAAGAGATGTTCAAAAAATGGACTACTTGAAATCGGTGGTGAAAGAAACTCTGAGGTTACACTCTCCAGCCCCTTTGCTCCCAAGAGAAGCAAGGGACACGGTTCAAATTAGCGGATTCGATGTACCAGTTAAATCAAAAGTAATTATTAATCTATGTGCAATAGGAAGAGACCCAGAGATGTGGGGAGCAGATGCTGAGTGTTTTAAGCCAGAGAGGTTTCATGGCTCTTCCGTTGACTTTAAGGGTTTTGACTTTGAGTTCATTCCGTTTGGGGCCGGCAGAAGAATATGTCCAGGCATGTCCTTTGGTGTTACCACGATTGAACTTGCTCTTGCTGAATTGCTCTACCGCTTCGATTGGAAACTAGCGAATGGGATGAATCCAGACGAACTTGACATGGCGGAGAGGTTTGGACTGAGCTGTAGGAGAAAGAATGCGTTGTACGTAATTGCCACCCCACATTTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00591 ko01100 ko04212 ko04726 ko04750 ko04913 map00590 map00591 map01100 map04212 map04726 map04750 map04913
Pfam Domains
Protein Families
p450

Protein Analysis

539

Amino Acids

60.44

Weight (kDa)

8.53

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 48 - 525 6.9e-94 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 339
AciI CCGC 7 cut(s) 359, 678, 875, 947, 1257, 1495, 1547
AclWI GGATC 4 cut(s) 111, 477, 733, 810
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 3 cut(s) 155, 1002, 1110
AfaI GTAC 6 cut(s) 504, 759, 775, 918, 1269, 1589
AfiI CCNNNNNNNGG 3 cut(s) 170, 259, 1035
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 8 cut(s) 316, 832, 955, 1142, 1157, 1174, 1250, 1469
AhdI GACNNNNNGTC 2 cut(s) 595, 1103
AjnI CCWGG 3 cut(s) 96, 145, 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
AluBI AGCT 8 cut(s) 20, 160, 299, 328, 451, 769, 1022, 1566
AluI AGCT 8 cut(s) 20, 160, 299, 328, 451, 769, 1022, 1566
Alw26I GTCTC 5 cut(s) 213, 540, 1007, 1090, 1308
AlwI GGATC 4 cut(s) 111, 477, 733, 810
AlwNI CAGNNNCTG 2 cut(s) 573, 1340
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 6 cut(s) 130, 149, 225, 273, 344, 1419
ApeKI GCWGC 5 cut(s) 160, 299, 573, 576, 605
ApoI RAATTY 1 cut(s) 904
ArsI GACNNNNNNTTYG 2 cut(s) 178, 210
AseI ATTAAT 1 cut(s) 1293
AspLEI GCGC 2 cut(s) 340, 461
AspS9I GGNCC 4 cut(s) 149, 194, 239, 1419
AsuC2I CCSGG 2 cut(s) 304, 305
AsuHPI GGTGA 3 cut(s) 112, 132, 1195
AsuNHI GCTAGC 1 cut(s) 674
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 2 cut(s) 194, 239
BalI TGGCCA 1 cut(s) 227
BanII GRGCYC 1 cut(s) 1084
BarI GAAGNNNNNNTAC 2 cut(s) 486, 518
BbsI GAAGAC 1 cut(s) 323
BbvI GCAGC 5 cut(s) 147, 286, 560, 563, 617
BccI CCATC 2 cut(s) 251, 560
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 3 cut(s) 98, 147, 1440
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 2 cut(s) 304, 305
BcoDI GTCTC 5 cut(s) 213, 540, 1007, 1090, 1308
BfaI CTAG 3 cut(s) 653, 675, 1511
BfmI CTRYAG 2 cut(s) 855, 1567
BfoI RGCGCY 1 cut(s) 462
BisI GCNGC 6 cut(s) 161, 300, 360, 574, 577, 606
BlsI GCNGC 6 cut(s) 162, 301, 361, 575, 578, 607
Bme1390I CCNGG 5 cut(s) 98, 147, 304, 305, 1440
Bme18I GGWCC 2 cut(s) 194, 239
BmeRI GACNNNNNGTC 2 cut(s) 595, 1103
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 4 cut(s) 149, 194, 239, 1419
BmiI GGNNCC 2 cut(s) 241, 1420
BmrFI CCNGG 5 cut(s) 98, 147, 304, 305, 1440
BmrI ACTGGG 2 cut(s) 269, 741
BmsI GCATC 3 cut(s) 102, 190, 1327
BmtI GCTAGC 1 cut(s) 678
BmuI ACTGGG 2 cut(s) 269, 741
BpiI GAAGAC 1 cut(s) 323
BpmI CTGGAG 1 cut(s) 1194
BpuMI CCSGG 2 cut(s) 304, 305
BsaAI YACGTR 1 cut(s) 1591
BsaI GGTCTC 1 cut(s) 1308
BsaJI CCNNGG 4 cut(s) 145, 146, 152, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 259, 1035
Bse118I RCCGGY 1 cut(s) 1421
Bse1I ACTGG 3 cut(s) 264, 736, 1271
Bse3DI GCAATG 2 cut(s) 871, 1047
BseBI CCWGG 3 cut(s) 98, 147, 1440
BseDI CCNNGG 4 cut(s) 145, 146, 152, 303
BseGI GGATG 2 cut(s) 93, 1527
BseLI CCNNNNNNNGG 3 cut(s) 170, 259, 1035
BseMI GCAATG 2 cut(s) 871, 1047
BseMII CTCAG 5 cut(s) 419, 724, 1187, 1332, 1553
BseNI ACTGG 3 cut(s) 264, 736, 1271
BseRI GAGGAG 7 cut(s) 893, 896, 899, 902, 905, 908, 911
BseXI GCAGC 5 cut(s) 147, 286, 560, 563, 617
BsgI GTGCAG 2 cut(s) 491, 1023
BshFI GGCC 6 cut(s) 132, 151, 227, 275, 346, 1421
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 2 cut(s) 304, 1422
BslFI GGGAC 2 cut(s) 558, 1253
BslI CCNNNNNNNGG 3 cut(s) 170, 259, 1035
BsmAI GTCTC 5 cut(s) 213, 540, 1007, 1090, 1308
BsmFI GGGAC 2 cut(s) 558, 1253
BsmI GAATGC 1 cut(s) 1585
BsnI GGCC 6 cut(s) 132, 151, 227, 275, 346, 1421
Bso31I GGTCTC 1 cut(s) 1308
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 1084
Bsp19I CCATGG 1 cut(s) 152
BspACI CCGC 7 cut(s) 359, 678, 875, 947, 1257, 1495, 1547
BspANI GGCC 6 cut(s) 132, 151, 227, 275, 346, 1421
BspCNI CTCAG 5 cut(s) 420, 723, 1188, 1333, 1554
BspHI TCATGA 1 cut(s) 1071
BspLI GGNNCC 2 cut(s) 241, 1420
BspOI GCTAGC 1 cut(s) 678
BspPI GGATC 4 cut(s) 111, 477, 733, 810
BspQI GCTCTTC 1 cut(s) 1378
BspTNI GGTCTC 1 cut(s) 1308
BsrDI GCAATG 2 cut(s) 871, 1047
BsrFI RCCGGY 1 cut(s) 1421
BsrI ACTGG 3 cut(s) 264, 736, 1271
BssAI RCCGGY 1 cut(s) 1421
BssECI CCNNGG 4 cut(s) 145, 146, 152, 303
BssT1I CCWWGG 1 cut(s) 152
Bst2UI CCWGG 3 cut(s) 98, 147, 1440
Bst4CI ACNGT 2 cut(s) 1101, 1246
Bst6I CTCTTC 4 cut(s) 492, 1070, 1306, 1378
BstBAI YACGTR 1 cut(s) 1591
BstC8I GCNNGC 5 cut(s) 330, 676, 767, 862, 1423
BstDEI CTNAG 7 cut(s) 428, 455, 710, 1030, 1196, 1341, 1562
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 2 cut(s) 93, 1527
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 2 cut(s) 340, 461
BstMAI GTCTC 5 cut(s) 213, 540, 1007, 1090, 1308
BstMWI GCNNNNNNNGC 3 cut(s) 157, 299, 671
BstNI CCWGG 3 cut(s) 98, 147, 1440
BstNSI RCATGY 1 cut(s) 1447
BstSCI CCNGG 5 cut(s) 96, 145, 302, 303, 1438
BstSFI CTRYAG 2 cut(s) 855, 1567
BstSNI TACGTA 1 cut(s) 1591
BstV1I GCAGC 5 cut(s) 147, 286, 560, 563, 617
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 482
BstXI CCANNNNNNTGG 2 cut(s) 153, 1326
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 6 cut(s) 132, 151, 227, 275, 346, 1421
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 2 cut(s) 93, 1527
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 197
Cac8I GCNNGC 5 cut(s) 330, 676, 767, 862, 1423
CaiI CAGNNNCTG 2 cut(s) 573, 1340
CciI TCATGA 1 cut(s) 1071
CfoI GCGC 2 cut(s) 340, 461
Cfr10I RCCGGY 1 cut(s) 1421
Cfr13I GGNCC 4 cut(s) 149, 194, 239, 1419
Cfr9I CCCGGG 1 cut(s) 303
Csp6I GTAC 6 cut(s) 503, 758, 774, 917, 1268, 1588
CviAII CATG 8 cut(s) 153, 236, 247, 323, 1072, 1367, 1444, 1543
CviQI GTAC 6 cut(s) 503, 758, 774, 917, 1268, 1588
DdeI CTNAG 7 cut(s) 428, 455, 710, 1030, 1196, 1341, 1562
DriI GACNNNNNGTC 2 cut(s) 595, 1103
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 4 cut(s) 492, 1070, 1306, 1378
Eam1105I GACNNNNNGTC 2 cut(s) 595, 1103
EarI CTCTTC 4 cut(s) 492, 1070, 1306, 1378
EciI GGCGGA 2 cut(s) 962, 1562
Eco105I TACGTA 1 cut(s) 1591
Eco130I CCWWGG 1 cut(s) 152
Eco147I AGGCCT 2 cut(s) 132, 346
Eco24I GRGCYC 1 cut(s) 1084
Eco31I GGTCTC 1 cut(s) 1308
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 2 cut(s) 194, 239
Eco57I CTGAAG 3 cut(s) 155, 1002, 1110
Eco88I CYCGRG 1 cut(s) 303
EcoRII CCWGG 3 cut(s) 96, 145, 1438
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 1 cut(s) 152
EcoT38I GRGCYC 1 cut(s) 1084
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 8 cut(s) 156, 239, 250, 326, 1075, 1370, 1447, 1546
FalI AAGNNNNNCTT 2 cut(s) 1000, 1032
FaqI GGGAC 2 cut(s) 558, 1253
FatI CATG 8 cut(s) 152, 235, 246, 322, 1071, 1366, 1443, 1542
FauI CCCGC 1 cut(s) 671
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 6 cut(s) 161, 300, 360, 574, 577, 606
FokI GGATG 2 cut(s) 80, 1534
FriOI GRGCYC 1 cut(s) 1084
Fsp4HI GCNGC 6 cut(s) 161, 300, 360, 574, 577, 606
FspBI CTAG 3 cut(s) 653, 675, 1511
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 2 cut(s) 339, 460
GluI GCNGC 6 cut(s) 161, 300, 360, 574, 577, 606
GsuI CTGGAG 1 cut(s) 1194
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 6 cut(s) 132, 151, 227, 275, 346, 1421
HapII CCGG 2 cut(s) 304, 1422
HhaI GCGC 2 cut(s) 340, 461
Hin1II CATG 8 cut(s) 156, 239, 250, 326, 1075, 1370, 1447, 1546
Hin6I GCGC 2 cut(s) 338, 459
HinP1I GCGC 2 cut(s) 338, 459
HincII GTYRAC 1 cut(s) 1381
HindII GTYRAC 1 cut(s) 1381
HinfI GANTC 9 cut(s) 28, 508, 518, 659, 723, 935, 1068, 1260, 1525
HpaII CCGG 2 cut(s) 304, 1422
HphI GGTGA 3 cut(s) 112, 132, 1195
Hpy166II GTNNAC 2 cut(s) 197, 1381
Hpy188I TCNGA 7 cut(s) 27, 482, 972, 1048, 1090, 1117, 1197
Hpy188III TCNNGA 6 cut(s) 120, 692, 782, 989, 1072, 1529
Hpy8I GTNNAC 2 cut(s) 197, 1381
HpyAV CCTTC 1 cut(s) 714
HpyCH4III ACNGT 2 cut(s) 1101, 1246
HpyCH4IV ACGT 4 cut(s) 466, 591, 756, 1590
HpyCH4V TGCA 9 cut(s) 93, 181, 250, 332, 441, 472, 624, 1004, 1304
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 299, 671
HpyF3I CTNAG 7 cut(s) 428, 455, 710, 1030, 1196, 1341, 1562
HpySE526I ACGT 4 cut(s) 466, 591, 756, 1590
Hsp92II CATG 8 cut(s) 156, 239, 250, 326, 1075, 1370, 1447, 1546
HspAI GCGC 2 cut(s) 338, 459
KroI GCCGGC 1 cut(s) 1421
KroNI GCCGGC 1 cut(s) 1423
Ksp22I TGATCA 1 cut(s) 12
LguI GCTCTTC 1 cut(s) 1378
LmnI GCTCC 3 cut(s) 406, 1227, 1331
Lsp1109I GCAGC 5 cut(s) 147, 286, 560, 563, 617
LweI GCATC 3 cut(s) 102, 190, 1327
MaeI CTAG 3 cut(s) 653, 675, 1511
MaeII ACGT 4 cut(s) 466, 591, 756, 1590
MaeIII GTNAC 4 cut(s) 138, 563, 1200, 1456
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 1084
MlsI TGGCCA 1 cut(s) 227
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 3 cut(s) 527, 944, 1077
Mox20I TGGCCA 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 1421
MscI TGGCCA 1 cut(s) 227
MseI TTAA 5 cut(s) 255, 1275, 1293, 1350, 1386
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 2 cut(s) 304, 1422
MspR9I CCNGG 5 cut(s) 98, 147, 304, 305, 1440
Mva1269I GAATGC 1 cut(s) 1585
MvaI CCWGG 3 cut(s) 98, 147, 1440
MwoI GCNNNNNNNGC 3 cut(s) 157, 299, 671
NaeI GCCGGC 1 cut(s) 1423
NciI CCSGG 2 cut(s) 304, 305
NcoI CCATGG 1 cut(s) 152
NgoMIV GCCGGC 1 cut(s) 1421
NheI GCTAGC 1 cut(s) 674
NlaIII CATG 8 cut(s) 156, 239, 250, 326, 1075, 1370, 1447, 1546
NlaIV GGNNCC 2 cut(s) 241, 1420
NmuCI GTSAC 2 cut(s) 138, 563
NsbI TGCGCA 1 cut(s) 339
NspI RCATGY 1 cut(s) 1447
PagI TCATGA 1 cut(s) 1071
PasI CCCWGGG 1 cut(s) 146
PceI AGGCCT 2 cut(s) 132, 346
PciSI GCTCTTC 1 cut(s) 1378
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 1 cut(s) 1585
PdiI GCCGGC 1 cut(s) 1423
PfeI GAWTC 6 cut(s) 28, 508, 659, 723, 1260, 1525
PkrI GCNGC 6 cut(s) 162, 301, 361, 575, 578, 607
PleI GAGTC 3 cut(s) 526, 943, 1076
PpsI GAGTC 3 cut(s) 526, 943, 1076
Ppu21I YACGTR 1 cut(s) 1591
PshBI ATTAAT 1 cut(s) 1293
Psp6I CCWGG 3 cut(s) 96, 145, 1438
PspGI CCWGG 3 cut(s) 96, 145, 1438
PspN4I GGNNCC 2 cut(s) 241, 1420
PspPI GGNCC 4 cut(s) 149, 194, 239, 1419
PstNI CAGNNNCTG 2 cut(s) 573, 1340
PsuI RGATCY 1 cut(s) 482
RsaI GTAC 6 cut(s) 504, 759, 775, 918, 1269, 1589
RsaNI GTAC 6 cut(s) 503, 758, 774, 917, 1268, 1588
RseI CAYNNNNRTG 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 1378
SaqAI TTAA 5 cut(s) 255, 1275, 1293, 1350, 1386
SatI GCNGC 6 cut(s) 161, 300, 360, 574, 577, 606
Sau96I GGNCC 4 cut(s) 149, 194, 239, 1419
SchI GAGTC 3 cut(s) 527, 944, 1077
ScrFI CCNGG 5 cut(s) 98, 147, 304, 305, 1440
SduI GDGCHC 1 cut(s) 1084
SfaNI GCATC 3 cut(s) 102, 190, 1327
SfcI CTRYAG 2 cut(s) 855, 1567
SinI GGWCC 2 cut(s) 194, 239
SmaI CCCGGG 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 98
SnaBI TACGTA 1 cut(s) 1591
SseBI AGGCCT 2 cut(s) 132, 346
SsiI CCGC 7 cut(s) 359, 678, 875, 947, 1257, 1495, 1547
SspMI CTAG 3 cut(s) 653, 675, 1511
StuI AGGCCT 2 cut(s) 132, 346
StyD4I CCNGG 5 cut(s) 96, 145, 302, 303, 1438
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 2 cut(s) 1101, 1246
TaiI ACGT 4 cut(s) 469, 594, 759, 1593
TaqI TCGA 6 cut(s) 51, 447, 737, 783, 1263, 1500
TaqII GACCGA 1 cut(s) 182
TauI GCSGC 1 cut(s) 362
TfiI GAWTC 6 cut(s) 28, 508, 659, 723, 1260, 1525
Tru1I TTAA 5 cut(s) 255, 1275, 1293, 1350, 1386
Tru9I TTAA 5 cut(s) 255, 1275, 1293, 1350, 1386
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 2 cut(s) 138, 563
TseI GCWGC 5 cut(s) 160, 299, 573, 576, 605
Tsp45I GTSAC 2 cut(s) 138, 563
TspDTI ATGAA 8 cut(s) 20, 276, 715, 764, 1088, 1355, 1396, 1538
TspGWI ACGGA 5 cut(s) 475, 520, 1134, 1366, 1401
TspMI CCCGGG 1 cut(s) 303
TspRI CASTG 1 cut(s) 204
VpaK11BI GGWCC 2 cut(s) 194, 239
VspI ATTAAT 1 cut(s) 1293
XapI RAATTY 1 cut(s) 904
XceI RCATGY 1 cut(s) 1447
XmaI CCCGGG 1 cut(s) 303
XspI CTAG 3 cut(s) 653, 675, 1511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.