Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 254 |
| Acc36I | ACCTGC | 1 cut(s) | 399 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 747 |
| AccIII | TCCGGA | 1 cut(s) | 184 |
| AclWI | GGATC | 1 cut(s) | 921 |
| AcsI | RAATTY | 2 cut(s) | 584, 821 |
| AcuI | CTGAAG | 2 cut(s) | 557, 611 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 595, 747, 936, 937 |
| AgsI | TTSAA | 8 cut(s) | 280, 408, 422, 459, 821, 887, 898, 918 |
| AjnI | CCWGG | 1 cut(s) | 856 |
| AluBI | AGCT | 3 cut(s) | 122, 666, 812 |
| AluI | AGCT | 3 cut(s) | 122, 666, 812 |
| Alw26I | GTCTC | 2 cut(s) | 112, 602 |
| AlwI | GGATC | 1 cut(s) | 921 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 122, 580 |
| Aor13HI | TCCGGA | 1 cut(s) | 184 |
| AoxI | GGCC | 1 cut(s) | 167 |
| ApeKI | GCWGC | 3 cut(s) | 79, 122, 394 |
| ApoI | RAATTY | 2 cut(s) | 584, 821 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 140, 172 |
| AseI | ATTAAT | 1 cut(s) | 675 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 765 |
| AspLEI | GCGC | 1 cut(s) | 255 |
| AspS9I | GGNCC | 1 cut(s) | 62 |
| AsuC2I | CCSGG | 1 cut(s) | 931 |
| AsuHPI | GGTGA | 3 cut(s) | 610, 706, 736 |
| AvaII | GGWCC | 1 cut(s) | 62 |
| BanII | GRGCYC | 1 cut(s) | 741 |
| BauI | CACGAG | 1 cut(s) | 498 |
| BbsI | GAAGAC | 1 cut(s) | 563 |
| BbvI | GCAGC | 3 cut(s) | 91, 109, 406 |
| BccI | CCATC | 2 cut(s) | 350, 499 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 140, 174 |
| BciT130I | CCWGG | 1 cut(s) | 858 |
| BclI | TGATCA | 3 cut(s) | 309, 600, 799 |
| BcnI | CCSGG | 1 cut(s) | 931 |
| BcoDI | GTCTC | 2 cut(s) | 112, 602 |
| BfaI | CTAG | 3 cut(s) | 84, 345, 438 |
| BfmI | CTRYAG | 1 cut(s) | 392 |
| BfuAI | ACCTGC | 1 cut(s) | 399 |
| BglII | AGATCT | 1 cut(s) | 258 |
| BisI | GCNGC | 3 cut(s) | 80, 123, 395 |
| BlpI | GCTNAGC | 1 cut(s) | 374 |
| BlsI | GCNGC | 3 cut(s) | 81, 124, 396 |
| Bme1390I | CCNGG | 2 cut(s) | 858, 931 |
| Bme18I | GGWCC | 1 cut(s) | 62 |
| BmgT120I | GGNCC | 1 cut(s) | 62 |
| BmiI | GGNNCC | 1 cut(s) | 64 |
| BmrFI | CCNGG | 2 cut(s) | 858, 931 |
| BoxI | GACNNNNGTC | 1 cut(s) | 347 |
| BpiI | GAAGAC | 1 cut(s) | 563 |
| BpmI | CTGGAG | 1 cut(s) | 262 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 374 |
| BpuEI | CTTGAG | 1 cut(s) | 58 |
| BpuMI | CCSGG | 1 cut(s) | 931 |
| BsaWI | WCCGGW | 1 cut(s) | 184 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 838, 868 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 595, 747, 936, 937 |
| Bse1I | ACTGG | 2 cut(s) | 245, 925 |
| Bse3DI | GCAATG | 2 cut(s) | 213, 480 |
| BseAI | TCCGGA | 1 cut(s) | 184 |
| BseBI | CCWGG | 1 cut(s) | 858 |
| BseGI | GGATG | 2 cut(s) | 361, 769 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 595, 747, 936, 937 |
| BseMI | GCAATG | 2 cut(s) | 213, 480 |
| BseNI | ACTGG | 2 cut(s) | 245, 925 |
| BseXI | GCAGC | 3 cut(s) | 91, 109, 406 |
| BsgI | GTGCAG | 2 cut(s) | 228, 858 |
| BshFI | GGCC | 1 cut(s) | 169 |
| BsiSI | CCGG | 2 cut(s) | 185, 931 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 595, 747, 936, 937 |
| BsmAI | GTCTC | 2 cut(s) | 112, 602 |
| BsmI | GAATGC | 1 cut(s) | 721 |
| BsnI | GGCC | 1 cut(s) | 169 |
| Bsp1286I | GDGCHC | 1 cut(s) | 741 |
| Bsp13I | TCCGGA | 1 cut(s) | 184 |
| Bsp143I | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 374 |
| BspANI | GGCC | 1 cut(s) | 169 |
| BspEI | TCCGGA | 1 cut(s) | 184 |
| BspLI | GGNNCC | 1 cut(s) | 64 |
| BspMAI | CTGCAG | 1 cut(s) | 396 |
| BspMI | ACCTGC | 1 cut(s) | 399 |
| BspPI | GGATC | 1 cut(s) | 921 |
| BsrDI | GCAATG | 2 cut(s) | 213, 480 |
| BsrI | ACTGG | 2 cut(s) | 245, 925 |
| BssMI | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| BssSI | CACGAG | 1 cut(s) | 498 |
| Bst2BI | CACGAG | 1 cut(s) | 498 |
| Bst2UI | CCWGG | 1 cut(s) | 858 |
| Bst4CI | ACNGT | 2 cut(s) | 100, 463 |
| Bst6I | CTCTTC | 4 cut(s) | 315, 636, 913, 929 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 215, 261 |
| BstC8I | GCNNGC | 1 cut(s) | 211 |
| BstDEI | CTNAG | 1 cut(s) | 374 |
| BstF5I | GGATG | 2 cut(s) | 361, 769 |
| BstHHI | GCGC | 1 cut(s) | 255 |
| BstKTI | GATC | 5 cut(s) | 261, 312, 603, 802, 929 |
| BstMAI | GTCTC | 2 cut(s) | 112, 602 |
| BstMBI | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 79, 175, 215, 261 |
| BstNI | CCWGG | 1 cut(s) | 858 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 347 |
| BstSCI | CCNGG | 2 cut(s) | 856, 929 |
| BstSFI | CTRYAG | 1 cut(s) | 392 |
| BstV1I | GCAGC | 3 cut(s) | 91, 109, 406 |
| BstV2I | GAAGAC | 1 cut(s) | 563 |
| BstX2I | RGATCY | 2 cut(s) | 258, 926 |
| BstYI | RGATCY | 2 cut(s) | 258, 926 |
| BsuRI | GGCC | 1 cut(s) | 169 |
| BtsCI | GGATG | 2 cut(s) | 361, 769 |
| BtsIMutI | CAGTG | 2 cut(s) | 96, 276 |
| BveI | ACCTGC | 1 cut(s) | 399 |
| Cac8I | GCNNGC | 1 cut(s) | 211 |
| CaiI | CAGNNNCTG | 2 cut(s) | 122, 580 |
| CfoI | GCGC | 1 cut(s) | 255 |
| Cfr13I | GGNCC | 1 cut(s) | 62 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 85, 120, 273, 308 |
| CviAII | CATG | 4 cut(s) | 146, 352, 720, 948 |
| CviJI | RGCY | 6 cut(s) | 82, 122, 169, 666, 739, 812 |
| CviKI_1 | RGCY | 6 cut(s) | 82, 122, 169, 666, 739, 812 |
| DdeI | CTNAG | 1 cut(s) | 374 |
| DpnI | GATC | 5 cut(s) | 260, 311, 602, 801, 928 |
| DpnII | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| Eam1104I | CTCTTC | 4 cut(s) | 315, 636, 913, 929 |
| EarI | CTCTTC | 4 cut(s) | 315, 636, 913, 929 |
| Eco24I | GRGCYC | 1 cut(s) | 741 |
| Eco32I | GATATC | 1 cut(s) | 190 |
| Eco47I | GGWCC | 1 cut(s) | 62 |
| Eco57I | CTGAAG | 2 cut(s) | 557, 611 |
| EcoRI | GAATTC | 1 cut(s) | 821 |
| EcoRII | CCWGG | 1 cut(s) | 856 |
| EcoRV | GATATC | 1 cut(s) | 190 |
| EcoT22I | ATGCAT | 1 cut(s) | 721 |
| EcoT38I | GRGCYC | 1 cut(s) | 741 |
| FaeI | CATG | 4 cut(s) | 149, 355, 723, 951 |
| FatI | CATG | 4 cut(s) | 145, 351, 719, 947 |
| FauNDI | CATATG | 1 cut(s) | 229 |
| FbaI | TGATCA | 3 cut(s) | 309, 600, 799 |
| Fnu4HI | GCNGC | 3 cut(s) | 80, 123, 395 |
| FokI | GGATG | 2 cut(s) | 368, 756 |
| FriOI | GRGCYC | 1 cut(s) | 741 |
| Fsp4HI | GCNGC | 3 cut(s) | 80, 123, 395 |
| FspBI | CTAG | 3 cut(s) | 84, 345, 438 |
| FspI | TGCGCA | 1 cut(s) | 254 |
| GlaI | GCGC | 1 cut(s) | 254 |
| GluI | GCNGC | 3 cut(s) | 80, 123, 395 |
| GsuI | CTGGAG | 1 cut(s) | 262 |
| HaeIII | GGCC | 1 cut(s) | 169 |
| HapII | CCGG | 2 cut(s) | 185, 931 |
| HhaI | GCGC | 1 cut(s) | 255 |
| Hin1II | CATG | 4 cut(s) | 149, 355, 723, 951 |
| Hin6I | GCGC | 1 cut(s) | 253 |
| HinP1I | GCGC | 1 cut(s) | 253 |
| HinfI | GANTC | 4 cut(s) | 244, 296, 348, 780 |
| HpaII | CCGG | 2 cut(s) | 185, 931 |
| HphI | GGTGA | 3 cut(s) | 610, 706, 736 |
| Hpy188III | TCNNGA | 5 cut(s) | 37, 185, 422, 589, 773 |
| HpyAV | CCTTC | 2 cut(s) | 586, 837 |
| HpyCH4III | ACNGT | 2 cut(s) | 100, 463 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 79, 175, 215, 261 |
| HpyF3I | CTNAG | 1 cut(s) | 374 |
| Hsp92II | CATG | 4 cut(s) | 149, 355, 723, 951 |
| HspAI | GCGC | 1 cut(s) | 253 |
| Kpn2I | TCCGGA | 1 cut(s) | 184 |
| Ksp22I | TGATCA | 3 cut(s) | 309, 600, 799 |
| Kzo9I | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| LmnI | GCTCC | 3 cut(s) | 229, 359, 809 |
| Lsp1109I | GCAGC | 3 cut(s) | 91, 109, 406 |
| MaeI | CTAG | 3 cut(s) | 84, 345, 438 |
| MaeIII | GTNAC | 1 cut(s) | 334 |
| MalI | GATC | 5 cut(s) | 260, 311, 602, 801, 928 |
| MboI | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| MboII | GAAGA | 9 cut(s) | 329, 332, 374, 447, 563, 623, 900, 936, 946 |
| MfeI | CAATTG | 1 cut(s) | 408 |
| MflI | RGATCY | 2 cut(s) | 258, 926 |
| MhlI | GDGCHC | 1 cut(s) | 741 |
| MluCI | AATT | 4 cut(s) | 265, 408, 584, 821 |
| MlyI | GAGTC | 3 cut(s) | 253, 305, 357 |
| MmeI | TCCRAC | 1 cut(s) | 459 |
| MnlI | CCTC | 8 cut(s) | 36, 229, 316, 517, 639, 645, 840, 930 |
| Mph1103I | ATGCAT | 1 cut(s) | 721 |
| MroI | TCCGGA | 1 cut(s) | 184 |
| MroXI | GAANNNNTTC | 1 cut(s) | 765 |
| MseI | TTAA | 1 cut(s) | 675 |
| MspI | CCGG | 2 cut(s) | 185, 931 |
| MspR9I | CCNGG | 2 cut(s) | 858, 931 |
| MunI | CAATTG | 1 cut(s) | 408 |
| Mva1269I | GAATGC | 1 cut(s) | 721 |
| MvaI | CCWGG | 1 cut(s) | 858 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 79, 175, 215, 261 |
| NciI | CCSGG | 1 cut(s) | 931 |
| NdeI | CATATG | 1 cut(s) | 229 |
| NdeII | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| NlaIII | CATG | 4 cut(s) | 149, 355, 723, 951 |
| NlaIV | GGNNCC | 1 cut(s) | 64 |
| NsbI | TGCGCA | 1 cut(s) | 254 |
| NsiI | ATGCAT | 1 cut(s) | 721 |
| PctI | GAATGC | 1 cut(s) | 721 |
| PdmI | GAANNNNTTC | 1 cut(s) | 765 |
| PfeI | GAWTC | 1 cut(s) | 780 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 747 |
| PfoI | TCCNGGA | 2 cut(s) | 856, 929 |
| PkrI | GCNGC | 3 cut(s) | 81, 124, 396 |
| PleI | GAGTC | 3 cut(s) | 252, 304, 356 |
| PpsI | GAGTC | 3 cut(s) | 252, 304, 356 |
| PshAI | GACNNNNGTC | 1 cut(s) | 347 |
| PshBI | ATTAAT | 1 cut(s) | 675 |
| Psp6I | CCWGG | 1 cut(s) | 856 |
| PspGI | CCWGG | 1 cut(s) | 856 |
| PspN4I | GGNNCC | 1 cut(s) | 64 |
| PspPI | GGNCC | 1 cut(s) | 62 |
| PstI | CTGCAG | 1 cut(s) | 396 |
| PstNI | CAGNNNCTG | 2 cut(s) | 122, 580 |
| PsuI | RGATCY | 2 cut(s) | 258, 926 |
| SaqAI | TTAA | 1 cut(s) | 675 |
| SatI | GCNGC | 3 cut(s) | 80, 123, 395 |
| Sau3AI | GATC | 5 cut(s) | 258, 309, 600, 799, 926 |
| Sau96I | GGNCC | 1 cut(s) | 62 |
| SchI | GAGTC | 3 cut(s) | 253, 305, 357 |
| ScrFI | CCNGG | 2 cut(s) | 858, 931 |
| SduI | GDGCHC | 1 cut(s) | 741 |
| SfcI | CTRYAG | 1 cut(s) | 392 |
| SinI | GGWCC | 1 cut(s) | 62 |
| SmlI | CTYRAG | 1 cut(s) | 37 |
| SmoI | CTYRAG | 1 cut(s) | 37 |
| Sse9I | AATT | 4 cut(s) | 265, 408, 584, 821 |
| SspI | AATATT | 2 cut(s) | 765, 817 |
| SspMI | CTAG | 3 cut(s) | 84, 345, 438 |
| StyD4I | CCNGG | 2 cut(s) | 856, 929 |
| TaaI | ACNGT | 2 cut(s) | 100, 463 |
| TasI | AATT | 4 cut(s) | 265, 408, 584, 821 |
| TfiI | GAWTC | 1 cut(s) | 780 |
| Tru1I | TTAA | 1 cut(s) | 675 |
| Tru9I | TTAA | 1 cut(s) | 675 |
| TscAI | CASTG | 2 cut(s) | 103, 276 |
| TseI | GCWGC | 3 cut(s) | 79, 122, 394 |
| TspDTI | ATGAA | 3 cut(s) | 576, 758, 814 |
| TspRI | CASTG | 2 cut(s) | 103, 276 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 747 |
| VpaK11BI | GGWCC | 1 cut(s) | 62 |
| VspI | ATTAAT | 1 cut(s) | 675 |
| XapI | RAATTY | 2 cut(s) | 584, 821 |
| XmnI | GAANNNNTTC | 1 cut(s) | 765 |
| XspI | CTAG | 3 cut(s) | 84, 345, 438 |
| Zsp2I | ATGCAT | 1 cut(s) | 721 |