Rw6G032520

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000132
Physical Location & Seq
Reverse (-)
56660762 .. 56663003
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032520.1

Sequence Viewer

Length: 1599 bp
ATGTGGTCCTTAATCCTCCAAACACTACCCATCAAAGCTCTTCCTCTTTCAGCTTATCTCCTCCTCTTAATCCTAGTGGTTCACTTTTGGAAGAGTTCCAAATTAGGCAAAAGCCAAGTCCTCAAGTCACCCCCAGGGCCATGGAAGCTGCCTATTCTAGGAAATTTGCTTCAGGTGGATGGTCATCCACTGCCACACTACCCATTGAGAGACCTAGCCAAGAAATATGGACCCATTATGCACCTGAAACTAGGTCAAACCGGCCCTGCGGAGGCTATTATAATTTCATCCCGCAAAATCACTTTTTCTCAGAGGCCTCTGGTTTTGCAAGTCGAAACCATCAACTTCGGCCGAGGAAGCATTGCATTTGCTCCTTATGGAGATTTCTGGAGAGAGGTGCGCAAGATTTGTGTTTCCGAATTATTAAGCGTTAAGCATGTGCAGGCATTTCGGTCCATAAGAGAAGAAGAGGTAAAGAACTTTGTTGAATCCATTTCCTCATCATCATCAAAATCATCAGCCATAAATTTCAGCGAGGAGAGCTTGCTCTTGACAAACGGCATAATATCGAGGGCAGCGTTTGGAAACAAGTGCAAATATCAAAAGGAGTTCCTAGCAGTGCTCGATGAAGCACTGAAACTCGGTGGAGGTTTTGACATACCTGATTTGTTTCCTTCACTTCGGTTTCTTTGTTTCGTTACTGGGACCATACCCGCGATGAGAAAGATGCGAGACAAGATTGGAGTAGCACTTGACAGTATCATCGATGATCATAAGAGGAAAAGATCATTGAAGGATGATAAACCAGCAGGAGCTGATCATGATGATGAAGAGGAGGAAGATCTAGTCGACGTACTTCTAAAACTCCAGGAGTCAAGTAATAAGCTTGATTTCAAGTTGACAACTGGTCAAATCAAAGATGTCGTTATGGAAATCTTCGTGGCGGGGAGTGAGACATCAGCTACAACCCTAGAATGGGCGATGTCGGAGTTGCTGAGAAACCCAAGAGTAATGAAGAAGGCCCAATCTGAGGTGCGACAATCAGTACTCCTTACAAGTGAACGAAAGAAATCAACAATAGAAGATACGACGTCTGACAATGTTCATTACAAATTGGATTACTTTAGATCAATTGTGAAAGAGACCCTGCGGTTGCACCCTCCTACTCCCTTGCTTTCAAGAGAATCAAGGGAAAGATGCGAAGTCGATGGATATGAATTACCTGCTAAGACGAAAGCAATCATCAATGTATGGGCACTGGCAAGAGATCCAGAACAATGGGGGGATGATGCTGATTCCTTTAAGCCAGAGAGGTTCCTCCATGACTCTATGACTGCCAAAATCGACTTCAGAGGGAATAACTTTGAGCTCTTACCCTTTGGGGCCGGTCGAAGAATATGTCCGGGCATGTCATTTGCCAATGCAGTGATTGAGCTAACTCTTTTCCAATTGCTCTACCGCTTTGATTGGGAACTGGCTGATGGGATAAAACCAGATGAACTAGACATGACTGAGAGTTGGGGAGCAACATGCAGGAAAAGGGACGATTTGTTCGTCATTGCCACCCCTCATTTCCTTGATTCAATGACCAAGTCCTAG

Protein Analysis

532

Amino Acids

60.14

Weight (kDa)

7.17

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 44 - 502 1.6e-80 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AatII GACGTC 1 cut(s) 1094
Acc16I TGCGCA 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 1231
AccI GTMKAC 1 cut(s) 849
AccII CGCG 1 cut(s) 716
AciI CCGC 6 cut(s) 269, 292, 714, 944, 1150, 1459
AclWI GGATC 1 cut(s) 1262
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 163, 526
AcuI CTGAAG 2 cut(s) 155, 1333
AcyI GRCGYC 1 cut(s) 1091
AfaI GTAC 2 cut(s) 855, 1047
AfiI CCNNNNNNNGG 5 cut(s) 158, 271, 975, 976, 1030
AgsI TTSAA 5 cut(s) 488, 793, 895, 1179, 1584
AhdI GACNNNNNGTC 1 cut(s) 906
AjnI CCWGG 2 cut(s) 133, 867
AjuI GAANNNNNNNTTGG 2 cut(s) 1331, 1363
AloI GAACNNNNNNTCC 2 cut(s) 1535, 1567
AluBI AGCT 9 cut(s) 38, 53, 148, 543, 815, 886, 962, 1369, 1435
AluI AGCT 9 cut(s) 38, 53, 148, 543, 815, 886, 962, 1369, 1435
Alw21I GWGCWC 2 cut(s) 624, 1371
Alw26I GTCTC 4 cut(s) 204, 726, 947, 1136
AlwI GGATC 1 cut(s) 1262
AlwNI CAGNNNCTG 1 cut(s) 815
AoxI GGCC 6 cut(s) 137, 262, 314, 349, 1020, 1383
ApeKI GCWGC 2 cut(s) 148, 575
ApoI RAATTY 2 cut(s) 163, 526
ArsI GACNNNNNNTTYG 6 cut(s) 102, 134, 1384, 1416, 1535, 1567
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 8 cut(s) 6, 137, 230, 263, 453, 705, 1021, 1383
AsuC2I CCSGG 1 cut(s) 1404
AsuHPI GGTGA 1 cut(s) 120
AvaII GGWCC 4 cut(s) 6, 230, 453, 705
BaeGI GKGCMC 1 cut(s) 1258
BanII GRGCYC 1 cut(s) 1371
Bbv12I GWGCWC 2 cut(s) 624, 1371
BbvI GCAGC 2 cut(s) 135, 587
BccI CCATC 5 cut(s) 38, 173, 347, 1202, 1475
BceAI ACGGC 1 cut(s) 574
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 2 cut(s) 135, 869
BclI TGATCA 2 cut(s) 769, 817
BcnI CCSGG 1 cut(s) 1404
BcoDI GTCTC 4 cut(s) 204, 726, 947, 1136
BfaI CTAG 9 cut(s) 74, 158, 215, 251, 614, 845, 971, 1502, 1597
BfuAI ACCTGC 1 cut(s) 1231
BglII AGATCT 1 cut(s) 841
BisI GCNGC 2 cut(s) 149, 576
BlsI GCNGC 2 cut(s) 150, 577
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 3 cut(s) 135, 869, 1404
Bme18I GGWCC 4 cut(s) 6, 230, 453, 705
BmeRI GACNNNNNGTC 1 cut(s) 906
BmgT120I GGNCC 8 cut(s) 6, 137, 230, 263, 453, 705, 1021, 1383
BmiI GGNNCC 4 cut(s) 232, 706, 1316, 1384
BmrFI CCNGG 3 cut(s) 135, 869, 1404
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 3 cut(s) 717, 1187, 1279
BmuI ACTGGG 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 409, 851
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 1404
Bsa29I ATCGAT 1 cut(s) 765
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 1091
BsaI GGTCTC 2 cut(s) 204, 1136
BsaJI CCNNGG 4 cut(s) 133, 134, 140, 352
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 271, 975, 976, 1030
Bse118I RCCGGY 2 cut(s) 260, 1385
Bse1I ACTGG 4 cut(s) 706, 910, 1263, 1479
Bse3DI GCAATG 2 cut(s) 360, 1557
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 2 cut(s) 135, 869
BseCI ATCGAT 1 cut(s) 765
BseDI CCNNGG 4 cut(s) 133, 134, 140, 352
BseGI GGATG 5 cut(s) 184, 184, 287, 802, 1291
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 5 cut(s) 158, 271, 975, 976, 1030
BseMI GCAATG 2 cut(s) 360, 1557
BseMII CTCAG 4 cut(s) 323, 986, 1020, 1503
BseNI ACTGG 4 cut(s) 706, 910, 1263, 1479
BseRI GAGGAG 4 cut(s) 50, 53, 551, 848
BseSI GKGCMC 1 cut(s) 1258
BseX3I CGGCCG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 135, 587
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 716
Bsh1285I CGRYCG 2 cut(s) 352, 1390
BshFI GGCC 6 cut(s) 139, 264, 316, 351, 1022, 1385
BshVI ATCGAT 1 cut(s) 765
BsiEI CGRYCG 2 cut(s) 352, 1390
BsiHKAI GWGCWC 2 cut(s) 624, 1371
BsiSI CCGG 3 cut(s) 261, 1386, 1403
BslFI GGGAC 2 cut(s) 718, 1556
BslI CCNNNNNNNGG 5 cut(s) 158, 271, 975, 976, 1030
BsmAI GTCTC 4 cut(s) 204, 726, 947, 1136
BsmFI GGGAC 2 cut(s) 718, 1556
BsnI GGCC 6 cut(s) 139, 264, 316, 351, 1022, 1385
Bso31I GGTCTC 2 cut(s) 204, 1136
Bsp1286I GDGCHC 3 cut(s) 624, 1258, 1371
Bsp143I GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 6 cut(s) 269, 292, 714, 944, 1150, 1459
BspANI GGCC 6 cut(s) 139, 264, 316, 351, 1022, 1385
BspCNI CTCAG 4 cut(s) 322, 987, 1021, 1504
BspDI ATCGAT 1 cut(s) 765
BspFNI CGCG 1 cut(s) 716
BspHI TCATGA 1 cut(s) 820
BspLI GGNNCC 4 cut(s) 232, 706, 1316, 1384
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 1 cut(s) 1262
BspQI GCTCTTC 1 cut(s) 45
BspTNI GGTCTC 2 cut(s) 204, 1136
BsrDI GCAATG 2 cut(s) 360, 1557
BsrFI RCCGGY 2 cut(s) 260, 1385
BsrI ACTGG 4 cut(s) 706, 910, 1263, 1479
BssAI RCCGGY 2 cut(s) 260, 1385
BssECI CCNNGG 4 cut(s) 133, 134, 140, 352
BssMI GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
BssNI GRCGYC 1 cut(s) 1091
BssT1I CCWWGG 1 cut(s) 140
Bst2UI CCWGG 2 cut(s) 135, 869
Bst4CI ACNGT 1 cut(s) 758
Bst6I CTCTTC 4 cut(s) 45, 86, 462, 825
BstACI GRCGYC 1 cut(s) 1091
BstC8I GCNNGC 2 cut(s) 444, 545
BstDEI CTNAG 5 cut(s) 309, 995, 1029, 1227, 1512
BstDSI CCRYGG 1 cut(s) 140
BstENI CCTNNNNNAGG 1 cut(s) 156
BstF5I GGATG 5 cut(s) 184, 184, 287, 802, 1291
BstFNI CGCG 1 cut(s) 716
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 6 cut(s) 772, 788, 820, 844, 1130, 1270
BstMAI GTCTC 4 cut(s) 204, 726, 947, 1136
BstMBI GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
BstMCI CGRYCG 2 cut(s) 352, 1390
BstMWI GCNNNNNNNGC 3 cut(s) 145, 357, 540
BstNI CCWGG 2 cut(s) 135, 869
BstNSI RCATGY 3 cut(s) 440, 1411, 1533
BstSCI CCNGG 3 cut(s) 133, 867, 1402
BstSLI GKGCMC 1 cut(s) 1258
BstUI CGCG 1 cut(s) 716
BstV1I GCAGC 2 cut(s) 135, 587
BstX2I RGATCY 2 cut(s) 841, 1267
BstXI CCANNNNNNTGG 2 cut(s) 141, 1278
BstYI RGATCY 2 cut(s) 841, 1267
BstZI CGGCCG 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 765
BsuRI GGCC 6 cut(s) 139, 264, 316, 351, 1022, 1385
BsuTUI ATCGAT 1 cut(s) 765
BtgI CCRYGG 1 cut(s) 140
BtgZI GCGATG 2 cut(s) 731, 995
BtsCI GGATG 5 cut(s) 184, 184, 287, 802, 1291
BtsI GCAGTG 3 cut(s) 188, 624, 1431
BtsIMutI CAGTG 5 cut(s) 188, 624, 632, 1256, 1431
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 2 cut(s) 444, 545
CaiI CAGNNNCTG 1 cut(s) 815
CciI TCATGA 1 cut(s) 820
CfoI GCGC 1 cut(s) 402
Cfr10I RCCGGY 2 cut(s) 260, 1385
Cfr13I GGNCC 8 cut(s) 6, 137, 230, 263, 453, 705, 1021, 1383
ClaI ATCGAT 1 cut(s) 765
Csp6I GTAC 2 cut(s) 854, 1046
CviAII CATG 7 cut(s) 141, 437, 821, 1322, 1408, 1507, 1530
CviQI GTAC 2 cut(s) 854, 1046
DdeI CTNAG 5 cut(s) 309, 995, 1029, 1227, 1512
DpnI GATC 6 cut(s) 771, 787, 819, 843, 1129, 1269
DpnII GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
DriI GACNNNNNGTC 1 cut(s) 906
EaeI YGGCCR 1 cut(s) 349
EagI CGGCCG 1 cut(s) 349
Eam1104I CTCTTC 4 cut(s) 45, 86, 462, 825
Eam1105I GACNNNNNGTC 1 cut(s) 906
EarI CTCTTC 4 cut(s) 45, 86, 462, 825
Ecl136II GAGCTC 1 cut(s) 1369
EclXI CGGCCG 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 140
Eco147I AGGCCT 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 1371
Eco31I GGTCTC 2 cut(s) 204, 1136
Eco47I GGWCC 4 cut(s) 6, 230, 453, 705
Eco52I CGGCCG 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 1369
Eco57I CTGAAG 2 cut(s) 155, 1333
EcoICRI GAGCTC 1 cut(s) 1369
EcoNI CCTNNNNNAGG 1 cut(s) 156
EcoRII CCWGG 2 cut(s) 133, 867
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 1371
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 7 cut(s) 144, 440, 824, 1325, 1411, 1510, 1533
FaqI GGGAC 2 cut(s) 718, 1556
FatI CATG 7 cut(s) 140, 436, 820, 1321, 1407, 1506, 1529
FauI CCCGC 3 cut(s) 299, 721, 937
FbaI TGATCA 2 cut(s) 769, 817
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 2 cut(s) 149, 576
FokI GGATG 5 cut(s) 171, 191, 274, 809, 1298
FriOI GRGCYC 1 cut(s) 1371
Fsp4HI GCNGC 2 cut(s) 149, 576
FspBI CTAG 9 cut(s) 74, 158, 215, 251, 614, 845, 971, 1502, 1597
FspI TGCGCA 1 cut(s) 401
GlaI GCGC 1 cut(s) 401
GluI GCNGC 2 cut(s) 149, 576
GsuI CTGGAG 2 cut(s) 409, 851
HaeIII GGCC 6 cut(s) 139, 264, 316, 351, 1022, 1385
HapII CCGG 3 cut(s) 261, 1386, 1403
HhaI GCGC 1 cut(s) 402
Hin1I GRCGYC 1 cut(s) 1091
Hin1II CATG 7 cut(s) 144, 440, 824, 1325, 1411, 1510, 1533
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HincII GTYRAC 2 cut(s) 850, 900
HindII GTYRAC 2 cut(s) 850, 900
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 6 cut(s) 488, 872, 1184, 1295, 1325, 1580
HpaII CCGG 3 cut(s) 261, 1386, 1403
HphI GGTGA 1 cut(s) 120
Hpy166II GTNNAC 4 cut(s) 82, 850, 900, 1061
Hpy188I TCNGA 6 cut(s) 312, 418, 988, 1030, 1096, 1352
Hpy188III TCNNGA 5 cut(s) 388, 550, 821, 1179, 1271
Hpy8I GTNNAC 4 cut(s) 82, 850, 900, 1061
Hpy99I CGWCG 2 cut(s) 854, 1093
HpyAV CCTTC 3 cut(s) 684, 787, 1012
HpyCH4III ACNGT 1 cut(s) 758
HpyCH4IV ACGT 2 cut(s) 852, 1091
HpyCH4V TGCA 8 cut(s) 241, 328, 365, 442, 594, 1156, 1424, 1533
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 357, 540
HpyF3I CTNAG 5 cut(s) 309, 995, 1029, 1227, 1512
HpySE526I ACGT 2 cut(s) 852, 1091
Hsp92I GRCGYC 1 cut(s) 1091
Hsp92II CATG 7 cut(s) 144, 440, 824, 1325, 1411, 1510, 1533
HspAI GCGC 1 cut(s) 400
Ksp22I TGATCA 2 cut(s) 769, 817
Kzo9I GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 3 cut(s) 376, 812, 1523
Lsp1109I GCAGC 2 cut(s) 135, 587
LweI GCATC 3 cut(s) 717, 1187, 1279
MaeI CTAG 9 cut(s) 74, 158, 215, 251, 614, 845, 971, 1502, 1597
MaeII ACGT 2 cut(s) 852, 1091
MaeIII GTNAC 2 cut(s) 126, 697
MalI GATC 6 cut(s) 771, 787, 819, 843, 1129, 1269
MboI GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
MfeI CAATTG 2 cut(s) 1131, 1448
MflI RGATCY 2 cut(s) 841, 1267
MhlI GDGCHC 3 cut(s) 624, 1258, 1371
MluCI AATT 9 cut(s) 101, 163, 282, 419, 526, 1112, 1131, 1217, 1448
MlyI GAGTC 2 cut(s) 881, 1319
MmeI TCCRAC 1 cut(s) 966
MseI TTAA 5 cut(s) 11, 68, 425, 432, 1302
MslI CAYNNNNRTG 1 cut(s) 825
MspI CCGG 3 cut(s) 261, 1386, 1403
MspR9I CCNGG 3 cut(s) 135, 869, 1404
MunI CAATTG 2 cut(s) 1131, 1448
MvaI CCWGG 2 cut(s) 135, 869
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 3 cut(s) 145, 357, 540
NciI CCSGG 1 cut(s) 1404
NcoI CCATGG 1 cut(s) 140
NdeII GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
NlaIII CATG 7 cut(s) 144, 440, 824, 1325, 1411, 1510, 1533
NlaIV GGNNCC 4 cut(s) 232, 706, 1316, 1384
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 126
NsbI TGCGCA 1 cut(s) 401
NspI RCATGY 3 cut(s) 440, 1411, 1533
PagI TCATGA 1 cut(s) 820
PasI CCCWGGG 1 cut(s) 134
PceI AGGCCT 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 2 cut(s) 575, 1551
PfeI GAWTC 4 cut(s) 488, 1184, 1295, 1580
PflFI GACNNNGTC 1 cut(s) 1591
PfoI TCCNGGA 1 cut(s) 867
PkrI GCNGC 2 cut(s) 150, 577
PleI GAGTC 2 cut(s) 880, 1319
PpsI GAGTC 2 cut(s) 880, 1319
PsiI TTATAA 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 1371
Psp6I CCWGG 2 cut(s) 133, 867
PspGI CCWGG 2 cut(s) 133, 867
PspN4I GGNNCC 4 cut(s) 232, 706, 1316, 1384
PspPI GGNCC 8 cut(s) 6, 137, 230, 263, 453, 705, 1021, 1383
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 2 cut(s) 841, 1267
PsyI GACNNNGTC 1 cut(s) 1591
RsaI GTAC 2 cut(s) 855, 1047
RsaNI GTAC 2 cut(s) 854, 1046
RseI CAYNNNNRTG 1 cut(s) 825
SacI GAGCTC 1 cut(s) 1371
SalI GTCGAC 1 cut(s) 848
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 5 cut(s) 11, 68, 425, 432, 1302
SatI GCNGC 2 cut(s) 149, 576
Sau3AI GATC 6 cut(s) 769, 785, 817, 841, 1127, 1267
Sau96I GGNCC 8 cut(s) 6, 137, 230, 263, 453, 705, 1021, 1383
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 2 cut(s) 881, 1319
ScrFI CCNGG 3 cut(s) 135, 869, 1404
SduI GDGCHC 3 cut(s) 624, 1258, 1371
SfaNI GCATC 3 cut(s) 717, 1187, 1279
SinI GGWCC 4 cut(s) 6, 230, 453, 705
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 9 cut(s) 101, 163, 282, 419, 526, 1112, 1131, 1217, 1448
SseBI AGGCCT 1 cut(s) 316
SsiI CCGC 6 cut(s) 269, 292, 714, 944, 1150, 1459
SspMI CTAG 9 cut(s) 74, 158, 215, 251, 614, 845, 971, 1502, 1597
SstI GAGCTC 1 cut(s) 1371
StuI AGGCCT 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 133, 867, 1402
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 758
TaiI ACGT 2 cut(s) 855, 1094
TaqI TCGA 8 cut(s) 333, 569, 624, 765, 849, 1206, 1344, 1390
TaqII GACCGA 1 cut(s) 441
TasI AATT 9 cut(s) 101, 163, 282, 419, 526, 1112, 1131, 1217, 1448
TatI WGTACW 1 cut(s) 1045
TfiI GAWTC 4 cut(s) 488, 1184, 1295, 1580
Tru1I TTAA 5 cut(s) 11, 68, 425, 432, 1302
Tru9I TTAA 5 cut(s) 11, 68, 425, 432, 1302
TscAI CASTG 5 cut(s) 195, 624, 639, 1263, 1431
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 2 cut(s) 148, 575
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 7 cut(s) 276, 642, 843, 1028, 1094, 1230, 1512
TspRI CASTG 5 cut(s) 195, 624, 639, 1263, 1431
Tth111I GACNNNGTC 1 cut(s) 1591
VpaK11BI GGWCC 4 cut(s) 6, 230, 453, 705
XagI CCTNNNNNAGG 1 cut(s) 156
XapI RAATTY 2 cut(s) 163, 526
XceI RCATGY 3 cut(s) 440, 1411, 1533
XmiI GTMKAC 1 cut(s) 849
XspI CTAG 9 cut(s) 74, 158, 215, 251, 614, 845, 971, 1502, 1597
ZraI GACGTC 1 cut(s) 1092
ZrmI AGTACT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.