Rroxscaffold_2G00077140

Premnaspirodiene oxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000132
Physical Location & Seq
Reverse (-)
784298 .. 786228
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077140.1

Sequence Viewer

Length: 1533 bp
ATGGCTTCTCCAATGTCCTTACTAATGCTCCAAATACCGTACTTCTCTCTCTTCACTTCTCTCTTGATCCTGGTTATTTCGTGGAAGAAATACAAAGCCAGATCAGGCTCTAATCCAAAGTCTCCACCAGGGCCATGGAAGCTGCCTATTATCGGAAACTTGCATCAGTTGGCTGCTGGTAATCCACTACCACATCATGCATTGAGAGACTTGGCCAAGAAACATGGACCTATTATGCGCCTAAAACTGGGTCAGGTGGAGGCTACTGTAATTTCTTCCTCCAAAGCTGCAGAAGAGGTCTTGAAGACACATGAGCTCACTTTTGCTCAGAGGCCTTTGGTTTTGGCTGTAGAAGTCCTGTCCTTTGCTCAATCAAGCATTGTGTTTTCTCCTTATGGTGATTACTGGAGACAGATGCGCAAGGTTTGTGTTCTCAAACTCTTCAACACTAAACGTGTACAGTCTTTCAGATCTATCAGAGAAGAAGAGGTTTTGAATTTCATCGAATCCATTCATTCTACATCATCAAAGGGACTTACTCCCATCAACTTCAGTGAGAAGATCTCCAGCTTGACAAATGGGATAGTGTCGAGGGCAGCGCTTGGTCAAAAGTGCAAAGATGAAAAGGAGTTTACTTCATTGCTCGAGGAAGCTACACAACTTGCTGCAGGCTTTGACATACCTGACTTGTTTCCTTCTCTCAGGTTTCTTGGTTATGTTACTGGGACAATACCTAAGATGAAGAAGATGCGAAACAATCTCGGAAAGATTCTTGAGAGGATCATCAATGATCACAAGATCAAAAGTCAAGGCAATGAGGTTAACAAGCCGGAGGAAGATTTTGTTGACGTACTTCTAAAACTTCAGGAGTCCAAGGAGCTTGAAGTAACAACTGATCAGATCAAAGATGTAATTATGGACGTATTCTCTGCAGGTAGTGAGACTACAGCCACGGCCATCGAATGGGCAATGTCGGAGTTGATGAGAAACCCTAGAGTCATGAACAAGGCCCAGGCTGAGATACGAAAACTCCTCCAAGGAAAGCCGAAAATTGAAGAAGCTGCAGACGTCATTCAAAACCTGGATTACTTGAAAGCAGTTGTGAAAGAAACTCTGAGAGAATCAAGAGAAAGATGCGAAATCAGTGGATACGAATTGCCAGAGAAAGCCAAAGTGATCATCAACGAATGGGCTATCGGAAGAGATCCAGAGAGTTGGGTTGATGCGGAGTCGTTTAAGCCAGAGAGGTTCCTGCATGGGTCTGAGTCAAATGTTGACTTTAAAGGTTTCGACTTTCAGTTCATTCCTTTTGGGGCTGGGAGAAGAATATGTCCGGGGATGTCATTTGGAGTTCCAATAATTGAAGTTGCTCTTTCTCAATTTCTCTACCATTTTGATTGGGAGCTGGGGAATGGGATCAAAGCAGAAGAGCTTGACATGTCTGAGACTTTCGGGATATCATGTAGGAGAAAGCATGATCTATACGTGATTGCCACCCCTCATCCTTTTCCTTCACTCAATGAGACATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00591 ko01100 ko04212 ko04726 ko04750 ko04913 map00590 map00591 map01100 map04212 map04726 map04750 map04913
Pfam Domains
Protein Families
p450

Protein Analysis

510

Amino Acids

57.54

Weight (kDa)

7.66

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 42 - 474 7.7e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1071
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 923
AccB7I CCANNNNNTGG 1 cut(s) 963
AciI CCGC 1 cut(s) 1226
AclWI GGATC 4 cut(s) 61, 788, 1199, 1424
AcoI YGGCCR 2 cut(s) 213, 954
AcsI RAATTY 1 cut(s) 496
AcuI CTGAAG 2 cut(s) 535, 848
AcyI GRCGYC 1 cut(s) 1068
AfaI GTAC 3 cut(s) 41, 459, 852
AfeI AGCGCT 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 152, 247, 963
AflIII ACRYGT 2 cut(s) 454, 1437
AgsI TTSAA 8 cut(s) 304, 445, 496, 884, 1055, 1076, 1093, 1364
AjnI CCWGG 4 cut(s) 69, 127, 1011, 1080
AluBI AGCT 9 cut(s) 142, 287, 316, 570, 653, 880, 1061, 1405, 1432
AluI AGCT 9 cut(s) 142, 287, 316, 570, 653, 880, 1061, 1405, 1432
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 6 cut(s) 126, 201, 403, 935, 1439, 1517
AlwI GGATC 4 cut(s) 61, 788, 1199, 1424
Ama87I CYCGRG 1 cut(s) 644
Aor51HI AGCGCT 1 cut(s) 600
AoxI GGCC 5 cut(s) 131, 213, 332, 954, 1008
ApeKI GCWGC 6 cut(s) 142, 173, 287, 596, 665, 1061
ApoI RAATTY 1 cut(s) 496
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 3 cut(s) 240, 420, 601
AspS9I GGNCC 3 cut(s) 131, 227, 1009
AsuC2I CCSGG 1 cut(s) 1335
AsuHPI GGTGA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 227
BalI TGGCCA 1 cut(s) 215
BanII GRGCYC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 6 cut(s) 129, 160, 274, 608, 652, 1048
BccI CCATC 2 cut(s) 551, 965
BceAI ACGGC 1 cut(s) 969
BciT130I CCWGG 4 cut(s) 71, 129, 1013, 1082
BciVI GTATCC 1 cut(s) 1142
BclI TGATCA 3 cut(s) 790, 895, 1176
BcnI CCSGG 1 cut(s) 1335
BcoDI GTCTC 6 cut(s) 126, 201, 403, 935, 1439, 1517
BfaI CTAG 1 cut(s) 993
BfmI CTRYAG 6 cut(s) 288, 348, 666, 930, 945, 1062
BfoI RGCGCY 1 cut(s) 602
BfuAI ACCTGC 1 cut(s) 923
BfuI GTATCC 1 cut(s) 1142
BglII AGATCT 2 cut(s) 470, 561
BisI GCNGC 6 cut(s) 143, 174, 288, 597, 666, 1062
BlsI GCNGC 6 cut(s) 144, 175, 289, 598, 667, 1063
Bme1390I CCNGG 5 cut(s) 71, 129, 1013, 1082, 1335
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 3 cut(s) 131, 227, 1009
BmiI GGNNCC 1 cut(s) 1250
BmrFI CCNGG 5 cut(s) 71, 129, 1013, 1082, 1335
BmrI ACTGGG 2 cut(s) 257, 732
BmsI GCATC 5 cut(s) 172, 405, 738, 1124, 1213
BmuI ACTGGG 2 cut(s) 257, 732
BpiI GAAGAC 1 cut(s) 311
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 2 cut(s) 427, 550
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 1335
BsaAI YACGTR 1 cut(s) 1486
BsaHI GRCGYC 1 cut(s) 1068
BsaJI CCNNGG 7 cut(s) 128, 134, 873, 951, 1011, 1036, 1334
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 1014, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 247, 963
Bse1I ACTGG 3 cut(s) 252, 410, 727
Bse3DI GCAATG 3 cut(s) 638, 820, 975
BseBI CCWGG 4 cut(s) 71, 129, 1013, 1082
BseDI CCNNGG 7 cut(s) 128, 134, 873, 951, 1011, 1036, 1334
BseGI GGATG 2 cut(s) 1344, 1501
BseLI CCNNNNNNNGG 3 cut(s) 152, 247, 963
BseMI GCAATG 3 cut(s) 638, 820, 975
BseMII CTCAG 6 cut(s) 341, 715, 1008, 1106, 1254, 1434
BseNI ACTGG 3 cut(s) 252, 410, 727
BseRI GAGGAG 1 cut(s) 1022
BseXI GCAGC 6 cut(s) 129, 160, 274, 608, 652, 1048
BseYI CCCAGC 2 cut(s) 1316, 1405
BshFI GGCC 5 cut(s) 133, 215, 334, 956, 1010
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 2 cut(s) 830, 1334
BslFI GGGAC 2 cut(s) 546, 739
BslI CCNNNNNNNGG 3 cut(s) 152, 247, 963
BsmAI GTCTC 6 cut(s) 126, 201, 403, 935, 1439, 1517
BsmFI GGGAC 2 cut(s) 546, 739
BsnI GGCC 5 cut(s) 133, 215, 334, 956, 1010
BsoBI CYCGRG 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 318
Bsp1407I TGTACA 1 cut(s) 457
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 1 cut(s) 1226
BspANI GGCC 5 cut(s) 133, 215, 334, 956, 1010
BspCNI CTCAG 6 cut(s) 340, 714, 1009, 1107, 1255, 1435
BspHI TCATGA 1 cut(s) 999
BspLI GGNNCC 1 cut(s) 1250
BspMAI CTGCAG 4 cut(s) 292, 670, 934, 1066
BspMI ACCTGC 1 cut(s) 923
BspPI GGATC 4 cut(s) 61, 788, 1199, 1424
BspQI GCTCTTC 1 cut(s) 1422
BsrDI GCAATG 3 cut(s) 638, 820, 975
BsrGI TGTACA 1 cut(s) 457
BsrI ACTGG 3 cut(s) 252, 410, 727
BssECI CCNNGG 7 cut(s) 128, 134, 873, 951, 1011, 1036, 1334
BssNI GRCGYC 1 cut(s) 1068
BssT1I CCWWGG 3 cut(s) 134, 873, 1036
Bst2UI CCWGG 4 cut(s) 71, 129, 1013, 1082
Bst4CI ACNGT 3 cut(s) 39, 268, 462
Bst6I CTCTTC 6 cut(s) 56, 288, 446, 480, 1195, 1422
BstACI GRCGYC 1 cut(s) 1068
BstAUI TGTACA 1 cut(s) 457
BstBAI YACGTR 1 cut(s) 1486
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 7 cut(s) 327, 701, 735, 1017, 1115, 1263, 1443
BstDSI CCRYGG 2 cut(s) 134, 951
BstF5I GGATG 2 cut(s) 1344, 1501
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 3 cut(s) 240, 420, 601
BstMAI GTCTC 6 cut(s) 126, 201, 403, 935, 1439, 1517
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 4 cut(s) 71, 129, 1013, 1082
BstNSI RCATGY 1 cut(s) 1441
BstSCI CCNGG 5 cut(s) 69, 127, 1011, 1080, 1333
BstSFI CTRYAG 6 cut(s) 288, 348, 666, 930, 945, 1062
BstV1I GCAGC 6 cut(s) 129, 160, 274, 608, 652, 1048
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 3 cut(s) 470, 561, 1204
BstXI CCANNNNNNTGG 2 cut(s) 135, 1215
BstYI RGATCY 3 cut(s) 470, 561, 1204
BsuI GTATCC 1 cut(s) 1142
BsuRI GGCC 5 cut(s) 133, 215, 334, 956, 1010
BtgI CCRYGG 2 cut(s) 134, 951
BtsCI GGATG 2 cut(s) 1344, 1501
BtsIMutI CAGTG 2 cut(s) 559, 1150
BveI ACCTGC 1 cut(s) 923
Cac8I GCNNGC 1 cut(s) 670
CciI TCATGA 1 cut(s) 999
CfoI GCGC 3 cut(s) 240, 420, 601
Cfr13I GGNCC 3 cut(s) 131, 227, 1009
Csp6I GTAC 3 cut(s) 40, 458, 851
CviAII CATG 9 cut(s) 135, 197, 224, 311, 1000, 1256, 1438, 1461, 1475
CviQI GTAC 3 cut(s) 40, 458, 851
DdeI CTNAG 7 cut(s) 327, 701, 735, 1017, 1115, 1263, 1443
DraI TTTAAA 1 cut(s) 1282
EaeI YGGCCR 2 cut(s) 213, 954
Eam1104I CTCTTC 6 cut(s) 56, 288, 446, 480, 1195, 1422
EarI CTCTTC 6 cut(s) 56, 288, 446, 480, 1195, 1422
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 3 cut(s) 134, 873, 1036
Eco147I AGGCCT 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 1458
Eco47I GGWCC 1 cut(s) 227
Eco47III AGCGCT 1 cut(s) 600
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 535, 848
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 316
EcoRII CCWGG 4 cut(s) 69, 127, 1011, 1080
EcoRV GATATC 1 cut(s) 1458
EcoT14I CCWWGG 3 cut(s) 134, 873, 1036
EcoT22I ATGCAT 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 3 cut(s) 134, 873, 1036
FaeI CATG 9 cut(s) 138, 200, 227, 314, 1003, 1259, 1441, 1464, 1478
FalI AAGNNNNNCTT 2 cut(s) 1356, 1388
FaqI GGGAC 2 cut(s) 546, 739
FatI CATG 9 cut(s) 134, 196, 223, 310, 999, 1255, 1437, 1460, 1474
FbaI TGATCA 3 cut(s) 790, 895, 1176
Fnu4HI GCNGC 6 cut(s) 143, 174, 288, 597, 666, 1062
FokI GGATG 2 cut(s) 1351, 1488
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 6 cut(s) 143, 174, 288, 597, 666, 1062
FspBI CTAG 1 cut(s) 993
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 3 cut(s) 239, 419, 600
GluI GCNGC 6 cut(s) 143, 174, 288, 597, 666, 1062
GsaI CCCAGC 2 cut(s) 1320, 1409
GsuI CTGGAG 2 cut(s) 427, 550
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 5 cut(s) 133, 215, 334, 956, 1010
HapII CCGG 2 cut(s) 830, 1334
HhaI GCGC 3 cut(s) 240, 420, 601
Hin1I GRCGYC 1 cut(s) 1068
Hin1II CATG 9 cut(s) 138, 200, 227, 314, 1003, 1259, 1441, 1464, 1478
Hin6I GCGC 3 cut(s) 238, 418, 599
HinP1I GCGC 3 cut(s) 238, 418, 599
HincII GTYRAC 3 cut(s) 823, 847, 1276
HindII GTYRAC 3 cut(s) 823, 847, 1276
HinfI GANTC 7 cut(s) 506, 769, 869, 996, 1121, 1229, 1265
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 2 cut(s) 830, 1334
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 5 cut(s) 458, 633, 823, 847, 1276
Hpy188III TCNNGA 8 cut(s) 64, 301, 773, 866, 1000, 1125, 1208, 1453
Hpy8I GTNNAC 5 cut(s) 458, 633, 823, 847, 1276
HpyAV CCTTC 2 cut(s) 705, 1521
HpyCH4III ACNGT 3 cut(s) 39, 268, 462
HpyCH4IV ACGT 5 cut(s) 454, 849, 921, 1068, 1485
HpyCH4V TGCA 8 cut(s) 163, 200, 290, 615, 668, 932, 1064, 1255
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 7 cut(s) 327, 701, 735, 1017, 1115, 1263, 1443
HpySE526I ACGT 5 cut(s) 454, 849, 921, 1068, 1485
Hsp92I GRCGYC 1 cut(s) 1068
Hsp92II CATG 9 cut(s) 138, 200, 227, 314, 1003, 1259, 1441, 1464, 1478
HspAI GCGC 3 cut(s) 238, 418, 599
Ksp22I TGATCA 3 cut(s) 790, 895, 1176
KspAI GTTAAC 1 cut(s) 823
LguI GCTCTTC 1 cut(s) 1422
LmnI GCTCC 3 cut(s) 33, 877, 1402
Lsp1109I GCAGC 6 cut(s) 129, 160, 274, 608, 652, 1048
LweI GCATC 5 cut(s) 172, 405, 738, 1124, 1213
MaeI CTAG 1 cut(s) 993
MaeII ACGT 5 cut(s) 454, 849, 921, 1068, 1485
MaeIII GTNAC 2 cut(s) 718, 886
MflI RGATCY 3 cut(s) 470, 561, 1204
MhlI GDGCHC 1 cut(s) 318
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 7 cut(s) 270, 496, 912, 1050, 1154, 1359, 1379
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 4 cut(s) 878, 1005, 1238, 1274
MmeI TCCRAC 1 cut(s) 954
Mox20I TGGCCA 1 cut(s) 215
Mph1103I ATGCAT 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 510
MscI TGGCCA 1 cut(s) 215
MseI TTAA 3 cut(s) 822, 1236, 1281
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 2 cut(s) 830, 1334
MspR9I CCNGG 5 cut(s) 71, 129, 1013, 1082, 1335
MvaI CCWGG 4 cut(s) 71, 129, 1013, 1082
MwoI GCNNNNNNNGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 1335
NcoI CCATGG 1 cut(s) 134
NlaIII CATG 9 cut(s) 138, 200, 227, 314, 1003, 1259, 1441, 1464, 1478
NlaIV GGNNCC 1 cut(s) 1250
NsbI TGCGCA 1 cut(s) 419
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 1 cut(s) 1441
PaeR7I CTCGAG 1 cut(s) 644
PagI TCATGA 1 cut(s) 999
PceI AGGCCT 1 cut(s) 334
PciI ACATGT 1 cut(s) 1437
PciSI GCTCTTC 1 cut(s) 1422
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 3 cut(s) 506, 769, 1121
PflMI CCANNNNNTGG 1 cut(s) 963
PkrI GCNGC 6 cut(s) 144, 175, 289, 598, 667, 1063
PleI GAGTC 4 cut(s) 877, 1004, 1237, 1273
PpsI GAGTC 4 cut(s) 877, 1004, 1237, 1273
Ppu21I YACGTR 1 cut(s) 1486
PscI ACATGT 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 4 cut(s) 69, 127, 1011, 1080
PspFI CCCAGC 2 cut(s) 1316, 1405
PspGI CCWGG 4 cut(s) 69, 127, 1011, 1080
PspN4I GGNNCC 1 cut(s) 1250
PspPI GGNCC 3 cut(s) 131, 227, 1009
PspXI VCTCGAGB 1 cut(s) 644
PstI CTGCAG 4 cut(s) 292, 670, 934, 1066
PsuI RGATCY 3 cut(s) 470, 561, 1204
RsaI GTAC 3 cut(s) 41, 459, 852
RsaNI GTAC 3 cut(s) 40, 458, 851
SacI GAGCTC 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 1422
SaqAI TTAA 3 cut(s) 822, 1236, 1281
SatI GCNGC 6 cut(s) 143, 174, 288, 597, 666, 1062
Sau96I GGNCC 3 cut(s) 131, 227, 1009
SchI GAGTC 4 cut(s) 878, 1005, 1238, 1274
ScrFI CCNGG 5 cut(s) 71, 129, 1013, 1082, 1335
SduI GDGCHC 1 cut(s) 318
SfaNI GCATC 5 cut(s) 172, 405, 738, 1124, 1213
SfcI CTRYAG 6 cut(s) 288, 348, 666, 930, 945, 1062
Sfr274I CTCGAG 1 cut(s) 644
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 644, 773
SmoI CTYRAG 2 cut(s) 644, 773
Sse9I AATT 7 cut(s) 270, 496, 912, 1050, 1154, 1359, 1379
SseBI AGGCCT 1 cut(s) 334
SsiI CCGC 1 cut(s) 1226
SspMI CTAG 1 cut(s) 993
SstI GAGCTC 1 cut(s) 318
StuI AGGCCT 1 cut(s) 334
StyD4I CCNGG 5 cut(s) 69, 127, 1011, 1080, 1333
StyI CCWWGG 3 cut(s) 134, 873, 1036
TaaI ACNGT 3 cut(s) 39, 268, 462
TaiI ACGT 5 cut(s) 457, 852, 924, 1071, 1488
TaqI TCGA 5 cut(s) 504, 590, 645, 960, 1290
TasI AATT 7 cut(s) 270, 496, 912, 1050, 1154, 1359, 1379
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 3 cut(s) 506, 769, 1121
Tru1I TTAA 3 cut(s) 822, 1236, 1281
Tru9I TTAA 3 cut(s) 822, 1236, 1281
TscAI CASTG 2 cut(s) 559, 1150
TseI GCWGC 6 cut(s) 142, 173, 287, 596, 665, 1061
TspDTI ATGAA 7 cut(s) 490, 503, 627, 636, 755, 1016, 1291
TspRI CASTG 2 cut(s) 559, 1150
Van91I CCANNNNNTGG 1 cut(s) 963
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 496
XceI RCATGY 1 cut(s) 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
XhoI CTCGAG 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 1 cut(s) 993
ZraI GACGTC 1 cut(s) 1069
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.