MD00G1153900.v1.1

Premnaspirodiene oxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000132
Physical Location & Seq
Reverse (-)
33648274 .. 33648705
432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1153900.v1.1.491

Sequence Viewer

Length: 432 bp
ATGCACCTTAAACTGGGTCAATTAGAGGCCGTGATTATTTCATCTCCCAAAGCTGCCCGGGAGGTGTTGAAGATTCATGAGCTTGCATTTGCGCAGAGGCCTATTGTTTTGGCGACAGAGGTTTTGTCTTTTGGTCAAGGAGGTATTATCTCTGCTCCTTATGGAGATTTATGGAGATCGCTGAGAAAGGTTTGTATGTTCGAGCTACTGAGCGCGAAACGTGTGCAGTCATTCAGATCCATAAGAGAAGAAGAGGCACAGAATCTTGTTGAGTCCATTGGCTCAATGTCTCACAAGGGACTTGCCATCAATTTTAGTGACAGGTGCTGCAGCTTTGCAAATGACGTGGTGTCAAAGGCAGCATTTGGGAAAAAAATGCAAAGACCAAAAGGAGTTCCTTTCGCTACTAGACGAAGTAAACAAACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00591 ko01100 ko04212 ko04726 ko04750 ko04913 map00590 map00591 map01100 map04212 map04726 map04750 map04913
Pfam Domains
Protein Families
p450

Protein Analysis

144

Amino Acids

15.91

Weight (kDa)

9.96

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 127 2.1e-13 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 93
AccII CGCG 1 cut(s) 215
AclWI GGATC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 13
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 70
AhdI GACNNNNNGTC 1 cut(s) 349
AjiI CACGTC 1 cut(s) 346
AluBI AGCT 4 cut(s) 53, 82, 205, 333
AluI AGCT 4 cut(s) 53, 82, 205, 333
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 231
AlwNI CAGNNNCTG 1 cut(s) 327
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 2 cut(s) 27, 98
ApeKI GCWGC 4 cut(s) 53, 327, 330, 359
ArsI GACNNNNNNTTYG 2 cut(s) 106, 138
AspLEI GCGC 2 cut(s) 94, 215
AsuC2I CCSGG 2 cut(s) 58, 59
AvaI CYCGRG 1 cut(s) 57
BbvI GCAGC 4 cut(s) 40, 314, 342, 371
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 14
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BcnI CCSGG 2 cut(s) 58, 59
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 2 cut(s) 408, 430
BfmI CTRYAG 1 cut(s) 328
BisI GCNGC 4 cut(s) 54, 328, 331, 360
BlsI GCNGC 4 cut(s) 55, 329, 332, 361
Bme1390I CCNGG 2 cut(s) 58, 59
BmeRI GACNNNNNGTC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 346
BmrFI CCNGG 2 cut(s) 58, 59
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BpuMI CCSGG 2 cut(s) 58, 59
BsaJI CCNNGG 1 cut(s) 57
Bsc4I CCNNNNNNNGG 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 173, 200
BseNI ACTGG 1 cut(s) 18
BseXI GCAGC 4 cut(s) 40, 314, 342, 371
BsgI GTGCAG 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 29, 100
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 13
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 2 cut(s) 29, 100
BsoBI CYCGRG 1 cut(s) 57
Bsp143I GATC 2 cut(s) 176, 236
BspANI GGCC 2 cut(s) 29, 100
BspCNI CTCAG 2 cut(s) 174, 201
BspFNI CGCG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 76
BspMAI CTGCAG 1 cut(s) 332
BspPI GGATC 1 cut(s) 231
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 2 cut(s) 176, 236
Bst6I CTCTTC 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 182, 209
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 2 cut(s) 94, 215
BstKTI GATC 2 cut(s) 179, 239
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 2 cut(s) 176, 236
BstSCI CCNGG 2 cut(s) 56, 57
BstSFI CTRYAG 1 cut(s) 328
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 40, 314, 342, 371
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BsuRI GGCC 2 cut(s) 29, 100
BtrI CACGTC 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 327
CciI TCATGA 1 cut(s) 76
CfoI GCGC 2 cut(s) 94, 215
Cfr9I CCCGGG 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 1 cut(s) 77
CviJI RGCY 7 cut(s) 29, 53, 82, 100, 205, 282, 333
CviKI_1 RGCY 7 cut(s) 29, 53, 82, 100, 205, 282, 333
DdeI CTNAG 2 cut(s) 182, 209
DpnI GATC 2 cut(s) 178, 238
DpnII GATC 2 cut(s) 176, 236
DriI GACNNNNNGTC 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 246
Eam1105I GACNNNNNGTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 246
Eco147I AGGCCT 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 57
FaeI CATG 1 cut(s) 80
FaiI YATR 5 cut(s) 78, 162, 172, 197, 242
FaqI GGGAC 1 cut(s) 312
FatI CATG 1 cut(s) 76
Fnu4HI GCNGC 4 cut(s) 54, 328, 331, 360
Fsp4HI GCNGC 4 cut(s) 54, 328, 331, 360
FspBI CTAG 2 cut(s) 408, 430
FspI TGCGCA 1 cut(s) 93
GlaI GCGC 2 cut(s) 93, 214
GluI GCNGC 4 cut(s) 54, 328, 331, 360
HaeIII GGCC 2 cut(s) 29, 100
HapII CCGG 1 cut(s) 58
HhaI GCGC 2 cut(s) 94, 215
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 2 cut(s) 92, 213
HinP1I GCGC 2 cut(s) 92, 213
HinfI GANTC 3 cut(s) 73, 262, 272
HpaII CCGG 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 1 cut(s) 236
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 419
HpyCH4IV ACGT 2 cut(s) 220, 345
HpyCH4V TGCA 6 cut(s) 4, 86, 226, 330, 338, 379
HpyF3I CTNAG 2 cut(s) 182, 209
HpySE526I ACGT 2 cut(s) 220, 345
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 2 cut(s) 92, 213
Kzo9I GATC 2 cut(s) 176, 236
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 2 cut(s) 71, 307
Lsp1109I GCAGC 4 cut(s) 40, 314, 342, 371
MaeI CTAG 2 cut(s) 408, 430
MaeII ACGT 2 cut(s) 220, 345
MaeIII GTNAC 1 cut(s) 317
MalI GATC 2 cut(s) 178, 238
MboI GATC 2 cut(s) 176, 236
MboII GAAGA 3 cut(s) 82, 260, 263
MflI RGATCY 1 cut(s) 236
MluCI AATT 2 cut(s) 20, 310
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 6 cut(s) 19, 55, 90, 112, 134, 247
MseI TTAA 1 cut(s) 9
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 58, 59
MvnI CGCG 1 cut(s) 215
NciI CCSGG 2 cut(s) 58, 59
NdeII GATC 2 cut(s) 176, 236
NlaIII CATG 1 cut(s) 80
NmuCI GTSAC 1 cut(s) 317
NsbI TGCGCA 1 cut(s) 93
PagI TCATGA 1 cut(s) 76
PceI AGGCCT 1 cut(s) 100
PfeI GAWTC 2 cut(s) 73, 262
PkrI GCNGC 4 cut(s) 55, 329, 332, 361
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PstI CTGCAG 1 cut(s) 332
PstNI CAGNNNCTG 1 cut(s) 327
PsuI RGATCY 1 cut(s) 236
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 4 cut(s) 54, 328, 331, 360
Sau3AI GATC 2 cut(s) 176, 236
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 2 cut(s) 58, 59
SfcI CTRYAG 1 cut(s) 328
SmaI CCCGGG 1 cut(s) 59
Sse9I AATT 2 cut(s) 20, 310
SseBI AGGCCT 1 cut(s) 100
SspMI CTAG 2 cut(s) 408, 430
StuI AGGCCT 1 cut(s) 100
StyD4I CCNGG 2 cut(s) 56, 57
TaiI ACGT 2 cut(s) 223, 348
TaqI TCGA 1 cut(s) 201
TasI AATT 2 cut(s) 20, 310
TfiI GAWTC 2 cut(s) 73, 262
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 4 cut(s) 53, 327, 330, 359
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 30, 65
TspMI CCCGGG 1 cut(s) 57
XmaI CCCGGG 1 cut(s) 57
XspI CTAG 2 cut(s) 408, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.