RchiOBHm_Chr2g0175711

Premnaspirodiene oxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000132
Physical Location & Seq
Forward (+)
87766940 .. 87771471
4532 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54276

Sequence Viewer

Length: 1542 bp
ATGTCCCTCTTAATGCTCCAAATACCCTACTTTCCTCTCTTTACTTCTCTCTTGATCCTGGTTATTGTTTGGAAAAAATCCAAAGCCAGATCAGGCCCTAGGAAGTCTCCACCAGGGCCATGGAAGCTGCCCATTATCGGAAACTTGCATCAGTTGGCTACTGGTAATCCTCTACCACATCATGCATTGAGAGACTTGGCCAAGAAACATGGACCTATTATGCACCTAAAACTGGGTCAGTTGGAGCTTATAATAATATCTTCCTCCAAAGCGGCACAAGAGGTTTTGAAGACACATGAGCTCACTTTTGCTCAGAGGCCTCTAGTTTTGGCAGTAGAAGTCATGTCCTTTGGTCAAGCAAGCATTGTCTTTGCTCCTTATGGTGATTTCTGGAGAGAGCTGCGAAAACTTTGTGTTCTCGAACTCTTCAGTGCTAAGCGTGTGCAATCTTTTAGATCGATAAGAGAAGAAGAGGTATCAAATCTCATCGAATCCATTTCTACATCATCAAACGGACCTACTCCGATCAACTTAAGTGAGAAGATCTCCAGCTTGACAAATGGAATAGTATCGAGGGCAGCGTTTGGAAAAAAGTGCAAAGATGAGAAAGAGTTTACTTCATTGCTTGAGGCAGCAATAAAACTGGCTGCAGGCTTTGACATACCTGACTTGTTTCCTTCACTAAAATTTCTTGGTTTCGTCACCGGGACGATACCTGCGATGAAGAAGATGAGGAACAAGCTTGGAAAGATCCTTGAGAGCATCATCAACGATCATAAGATCAAAATTCAAGGCAATGAGATTAACAACCCGGAAGAAGATATTGTTGACGTACTTCTAAAACTTCAAGAATCCAAGGAGCTTGAATTCACAACCGATCAGATCAAAGATGTAATTATGGATATATTCTCTGCAGGTAGTGAGACTACAGCTACTACCATCGAATGGGCAATGTCGGAGTTGATGAAAAACCCTAGCGTAATGAACAAGGCCCATGCTGAGCTGCGAAAACTCCTCGAAGGAAAGCCAAAAATTGAAGAAGTAGACGTTCAAAACCTGGATTACTTGAAAGCAGTTGTGAAAGAAACTCTGAGACTGCACCCTCCAGGCACATTACTACCAAGAGAATCAAGAGAAAGTTGCGAAATCGGCGGATACGCATTCCCAGCCAAAACGAAAGTGCTCATCAACGAGTGGGCAATTGGAAGAGATCCGGAGTGTTGGATTGATGCAGAGTCGTTTAAGCCAGAGAGGTTTATGCATGGTTCTAAATCAAATGTTGACTTTAAAGGTTTCGATTTTGAGTTCATTCCCTTTGGAGCTGGGAGAAGAATATGTCCGGGGATGTCATTTGGAGTTTCAATCATTGAAGTTGCTCTTTCTCAATTGCTTTACCATTTTGATTGGGAGTTGGGGAATGGGATTAAACCAGATGAACTTGACATGTCCGAGGCCTTCGGGATAACATGTAGGAGAAAGCATGATCTGTACGTGATTGCTACGCCTCATCGTTTTCCTGCACTCCATAATGAGACTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00591 ko01100 ko04212 ko04726 ko04750 ko04913 map00590 map00591 map01100 map04212 map04726 map04750 map04913
Pfam Domains
Protein Families
p450

Protein Analysis

513

Amino Acids

57.57

Weight (kDa)

7.67

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 37 - 482 1.4e-98 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03210 FvH4_3g24170 FvH4_3g24171 FvH4_6g02110 FvH4_6g02110 FvH4_6g21500 FvH4_6g44530 FvH4_6g44700 FvH4_6g44700 FvH4_6g44710 FvH4_6g44730 FvH4_6g44750 FvH4_6g44751 FvH4_6g44751 FvH4_6g53570
malus_domestica MD00G1138500.v1.1 MD00G1138600.v1.1 MD00G1138700.v1.1 MD00G1153200.v1.1 MD00G1153300.v1.1 MD00G1153400.v1.1 MD00G1153500.v1.1 MD00G1153600.v1.1 MD00G1153800.v1.1 MD00G1153900.v1.1 MD00G1154000.v1.1 MD03G1187300.v1.1 MD04G1245500.v1.1 MD11G1202200.v1.1 MD11G1202300.v1.1 MD11G1202500.v1.1 MD14G1096600.v1.1 MD14G1096700.v1.1 MD17G1000100.v1.1 MD17G1000200.v1.1 MD17G1000300.v1.1
prunus_persica Prupe.1G492000_v2.0.a1 Prupe.1G492100_v2.0.a1 Prupe.2G073800_v2.0.a1 Prupe.2G073900_v2.0.a1 Prupe.2G074300_v2.0.a1 Prupe.3G306800_v2.0.a1 Prupe.4G237900_v2.0.a1 Prupe.4G238000_v2.0.a1 Prupe.4G238200_v2.0.a1 Prupe.4G238300_v2.0.a1 Prupe.4G238600_v2.0.a1 Prupe.4G239200_v2.0.a1 Prupe.4G239300_v2.0.a1
pyrus_communis pycom03g13950 pycom03g14010 pycom04g21620 pycom04g21630 pycom111g00820 pycom11g17490 pycom11g17500 pycom17g00860 pycom17g00880
rosa_chinensis RchiOBHm_Chr1g0325151 RchiOBHm_Chr1g0329431 RchiOBHm_Chr1g0334551 RchiOBHm_Chr1g0334561 RchiOBHm_Chr1g0336531 RchiOBHm_Chr1g0336541 RchiOBHm_Chr2g0175621 RchiOBHm_Chr2g0175641 RchiOBHm_Chr2g0175651 RchiOBHm_Chr2g0175701 RchiOBHm_Chr2g0175711 RchiOBHm_Chr3g0447771 RchiOBHm_Chr3g0447781 RchiOBHm_Chr3g0447791 RchiOBHm_Chr3g0447801 RchiOBHm_Chr5g0043061 RchiOBHm_Chr5g0043071 RchiOBHm_Chr5g0043221 RchiOBHm_Chr5g0043231 RchiOBHm_Chr5g0043921 RchiOBHm_Chr5g0054481 RchiOBHm_Chr5g0054491 RchiOBHm_Chr5g0054511 RchiOBHm_Chr5g0054551 RchiOBHm_Chr5g0054561 RchiOBHm_Chr5g0054581 RchiOBHm_Chr6g0296341
rosa_laevigata RLG00000011704 RLG00000022344 RLG00000022345 RLG00000022350 RLG00000022351 RLG00000025970 RLG00000025971 RLG00000025972 RLG00000029355 RLG00000029356 RLG00000029886 RLG00000030155 RLG00000034172 RLG00000034938
rosa_multiflora Rmu_co8138254.1_g000001 Rmu_co8268883.1_g000001 Rmu_co8271167.1_g000001 Rmu_co8294571.1_g000001 Rmu_co8350865.1_g000001 Rmu_co8364355.1_g000001 Rmu_co8416839.1_g000001 Rmu_sc0000698.1_g000089 Rmu_sc0000698.1_g000146 Rmu_sc0000998.1_g000014 Rmu_sc0000998.1_g000015 Rmu_sc0000998.1_g000020 Rmu_sc0000998.1_g000021 Rmu_sc0001478.1_g000005 Rmu_sc0001654.1_g000011 Rmu_sc0001981.1_g000002 Rmu_sc0002655.1_g000005 Rmu_sc0002655.1_g000011 Rmu_sc0002655.1_g000018 Rmu_sc0005044.1_g000025 Rmu_sc0005591.1_g000001 Rmu_sc0007742.1_g000012 Rmu_sc0008148.1_g000071 Rmu_sc0009324.1_g000003 Rmu_sc0009324.1_g000004 Rmu_sc0009324.1_g000005 Rmu_sc0014424.1_g000003 Rmu_sc0019317.1_g000001 Rmu_sc0027639.1_g000001
rosa_roxburghii Rroxscaffold_1G00026360 Rroxscaffold_1G00026400 Rroxscaffold_1G00037560 Rroxscaffold_2G00077140 Rroxscaffold_2G00077180 Rroxscaffold_4G00315510 Rroxscaffold_4G00315520 Rroxscaffold_4G00324880 Rroxscaffold_6G00425980 Rroxscaffold_6G00425990 Rroxscaffold_7G00171610 Rroxscaffold_7G00178470 Rroxscaffold_7G00178490
rosa_rugosa Rorug01G0050000 Rorug01G0079800 Rorug01G0126000 Rorug01G0126400 Rorug02G0586700 Rorug02G0586700 Rorug02G0586800 Rorug02G0587000 Rorug02G0605600 Rorug02G0605700 Rorug05G0123000 Rorug05G0204600 Rorug05G0204800 Rorug05G0209100 Rorug05G0286600 Rorug05G0286800 Rorug05G0286900 Rorug06G0261200 Rorug07G0200800
rosa_samantha Rh1AG098700 Rh1BG054000 Rh1BG078400 Rh1BG078500 Rh1BG078600 Rh1BG115300 Rh1CG094700 Rh1CG138500 Rh2BG676800 Rh2BG677200 Rh2CG639900 Rh2CG640000 Rh2CG640100 Rh2CG640400 Rh2CG640500 Rh2DG690700 Rh2DG691000 Rh2DG691100 Rh3AG005200 Rh3BG004800 Rh3BG004900 Rh3DG005100 Rh3DG005300 Rh5AG290500 Rh5AG357000 Rh5AG357100 Rh5AG357300 Rh5BG296700 Rh5BG369300 Rh5CG326700 Rh5CG392000 Rh5DG310400 Rh6BG381100 Rh6DG373800
rosa_wichuraiana Rw0G010260 Rw1G007710 Rw1G009530 Rw1G011920 Rw2G004130 Rw2G054610 Rw3G000390 Rw5G026850 Rw5G026940 Rw5G033530 Rw5G033720 Rw5G033730 Rw5G033760 Rw6G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 724, 905
AccB7I CCANNNNNTGG 1 cut(s) 945
AccI GTMKAC 1 cut(s) 1044
AccIII TCCGGA 1 cut(s) 1213
AciI CCGC 2 cut(s) 272, 1152
AclWI GGATC 3 cut(s) 49, 745, 1205
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 3 cut(s) 686, 786, 866
AcuI CTGAAG 1 cut(s) 412
AfaI GTAC 2 cut(s) 834, 1490
AfiI CCNNNNNNNGG 3 cut(s) 137, 232, 945
AflII CTTAAG 1 cut(s) 532
AflIII ACRYGT 2 cut(s) 1443, 1466
AgsI TTSAA 9 cut(s) 289, 791, 848, 866, 1037, 1052, 1069, 1362, 1370
AjnI CCWGG 4 cut(s) 57, 112, 1056, 1105
Alw21I GWGCWC 2 cut(s) 303, 1185
Alw26I GTCTC 5 cut(s) 111, 186, 917, 1087, 1526
AlwI GGATC 3 cut(s) 49, 745, 1205
Aor13HI TCCGGA 1 cut(s) 1213
AoxI GGCC 6 cut(s) 94, 116, 198, 317, 990, 1452
ApeKI GCWGC 6 cut(s) 127, 400, 578, 632, 647, 1003
ApoI RAATTY 3 cut(s) 686, 786, 866
AspA2I CCTAGG 1 cut(s) 98
AspS9I GGNCC 5 cut(s) 95, 116, 212, 515, 991
AsuC2I CCSGG 3 cut(s) 706, 812, 1341
AsuHPI GGTGA 2 cut(s) 395, 694
AvaII GGWCC 2 cut(s) 212, 515
AvrII CCTAGG 1 cut(s) 98
BalI TGGCCA 1 cut(s) 200
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 303, 1185
BbvI GCAGC 6 cut(s) 114, 387, 590, 634, 644, 990
BccI CCATC 1 cut(s) 947
BciT130I CCWGG 4 cut(s) 59, 114, 1058, 1107
BciVI GTATCC 1 cut(s) 1148
BcnI CCSGG 3 cut(s) 706, 812, 1341
BcoDI GTCTC 5 cut(s) 111, 186, 917, 1087, 1526
BfaI CTAG 3 cut(s) 99, 323, 975
BfmI CTRYAG 3 cut(s) 648, 912, 927
BfrI CTTAAG 1 cut(s) 532
BfuAI ACCTGC 2 cut(s) 724, 905
BfuI GTATCC 1 cut(s) 1148
BglII AGATCT 1 cut(s) 543
BisI GCNGC 7 cut(s) 128, 273, 401, 579, 633, 648, 1004
BlnI CCTAGG 1 cut(s) 98
BlpI GCTNAGC 2 cut(s) 435, 999
BlsI GCNGC 7 cut(s) 129, 274, 402, 580, 634, 649, 1005
Bme1390I CCNGG 7 cut(s) 59, 114, 706, 812, 1058, 1107, 1341
Bme18I GGWCC 2 cut(s) 212, 515
BmgT120I GGNCC 5 cut(s) 95, 116, 212, 515, 991
BmrFI CCNGG 7 cut(s) 59, 114, 706, 812, 1058, 1107, 1341
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 3 cut(s) 157, 771, 1219
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 296
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 3 cut(s) 412, 532, 1089
Bpu1102I GCTNAGC 2 cut(s) 435, 999
BpuEI CTTGAG 2 cut(s) 647, 776
BpuMI CCSGG 3 cut(s) 706, 812, 1341
Bsa29I ATCGAT 1 cut(s) 458
BsaAI YACGTR 1 cut(s) 1492
BsaJI CCNNGG 6 cut(s) 98, 113, 119, 855, 1340, 1449
BsaWI WCCGGW 1 cut(s) 1213
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 232, 945
Bse1I ACTGG 3 cut(s) 166, 237, 648
Bse3DI GCAATG 3 cut(s) 620, 802, 957
BseAI TCCGGA 1 cut(s) 1213
BseBI CCWGG 4 cut(s) 59, 114, 1058, 1107
BseCI ATCGAT 1 cut(s) 458
BseDI CCNNGG 6 cut(s) 98, 113, 119, 855, 1340, 1449
BseGI GGATG 1 cut(s) 1350
BseLI CCNNNNNNNGG 3 cut(s) 137, 232, 945
BseMI GCAATG 3 cut(s) 620, 802, 957
BseMII CTCAG 3 cut(s) 326, 990, 1082
BseNI ACTGG 3 cut(s) 166, 237, 648
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 6 cut(s) 114, 387, 590, 634, 644, 990
BseYI CCCAGC 2 cut(s) 1165, 1322
BsgI GTGCAG 2 cut(s) 1082, 1503
BshFI GGCC 6 cut(s) 96, 118, 200, 319, 992, 1454
BshVI ATCGAT 1 cut(s) 458
BsiHKAI GWGCWC 2 cut(s) 303, 1185
BsiSI CCGG 4 cut(s) 705, 812, 1214, 1340
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 3 cut(s) 137, 232, 945
BsmAI GTCTC 5 cut(s) 111, 186, 917, 1087, 1526
BsmFI GGGAC 1 cut(s) 721
BsmI GAATGC 1 cut(s) 1160
BsnI GGCC 6 cut(s) 96, 118, 200, 319, 992, 1454
Bsp1286I GDGCHC 2 cut(s) 303, 1185
Bsp13I TCCGGA 1 cut(s) 1213
Bsp1720I GCTNAGC 2 cut(s) 435, 999
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 2 cut(s) 272, 1152
BspANI GGCC 6 cut(s) 96, 118, 200, 319, 992, 1454
BspCNI CTCAG 3 cut(s) 325, 991, 1083
BspDI ATCGAT 1 cut(s) 458
BspEI TCCGGA 1 cut(s) 1213
BspMAI CTGCAG 2 cut(s) 652, 916
BspMI ACCTGC 2 cut(s) 724, 905
BspPI GGATC 3 cut(s) 49, 745, 1205
BspTI CTTAAG 1 cut(s) 532
BsrDI GCAATG 3 cut(s) 620, 802, 957
BsrI ACTGG 3 cut(s) 166, 237, 648
BssECI CCNNGG 6 cut(s) 98, 113, 119, 855, 1340, 1449
BssT1I CCWWGG 3 cut(s) 98, 119, 855
Bst2UI CCWGG 4 cut(s) 59, 114, 1058, 1107
Bst6I CTCTTC 3 cut(s) 431, 465, 1201
BstAFI CTTAAG 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 1492
BstC8I GCNNGC 2 cut(s) 361, 652
BstDEI CTNAG 4 cut(s) 312, 435, 999, 1091
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 1350
BstMAI GTCTC 5 cut(s) 111, 186, 917, 1087, 1526
BstMWI GCNNNNNNNGC 3 cut(s) 124, 1149, 1166
BstNI CCWGG 4 cut(s) 59, 114, 1058, 1107
BstNSI RCATGY 2 cut(s) 1447, 1470
BstSCI CCNGG 7 cut(s) 57, 112, 704, 810, 1056, 1105, 1339
BstSFI CTRYAG 3 cut(s) 648, 912, 927
BstV1I GCAGC 6 cut(s) 114, 387, 590, 634, 644, 990
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 3 cut(s) 543, 750, 1210
BstXI CCANNNNNNTGG 1 cut(s) 120
BstYI RGATCY 3 cut(s) 543, 750, 1210
Bsu15I ATCGAT 1 cut(s) 458
BsuI GTATCC 1 cut(s) 1148
BsuRI GGCC 6 cut(s) 96, 118, 200, 319, 992, 1454
BsuTUI ATCGAT 1 cut(s) 458
BtgI CCRYGG 1 cut(s) 119
BtgZI GCGATG 1 cut(s) 734
BtsCI GGATG 1 cut(s) 1350
BtsIMutI CAGTG 1 cut(s) 436
BveI ACCTGC 2 cut(s) 724, 905
Cac8I GCNNGC 2 cut(s) 361, 652
Cfr13I GGNCC 5 cut(s) 95, 116, 212, 515, 991
ClaI ATCGAT 1 cut(s) 458
Csp6I GTAC 2 cut(s) 833, 1489
CviQI GTAC 2 cut(s) 833, 1489
DdeI CTNAG 4 cut(s) 312, 435, 999, 1091
DraI TTTAAA 1 cut(s) 1288
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 3 cut(s) 431, 465, 1201
EarI CTCTTC 3 cut(s) 431, 465, 1201
EciI GGCGGA 1 cut(s) 1167
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 3 cut(s) 98, 119, 855
Eco147I AGGCCT 2 cut(s) 319, 1454
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 2 cut(s) 212, 515
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 4 cut(s) 57, 112, 1056, 1105
EcoT14I CCWWGG 3 cut(s) 98, 119, 855
EcoT22I ATGCAT 2 cut(s) 187, 1263
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 3 cut(s) 98, 119, 855
FalI AAGNNNNNCTT 2 cut(s) 1362, 1394
FaqI GGGAC 1 cut(s) 721
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 7 cut(s) 128, 273, 401, 579, 633, 648, 1004
FokI GGATG 1 cut(s) 1357
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 7 cut(s) 128, 273, 401, 579, 633, 648, 1004
FspBI CTAG 3 cut(s) 99, 323, 975
GluI GCNGC 7 cut(s) 128, 273, 401, 579, 633, 648, 1004
GsaI CCCAGC 2 cut(s) 1169, 1326
GsuI CTGGAG 3 cut(s) 412, 532, 1089
HaeIII GGCC 6 cut(s) 96, 118, 200, 319, 992, 1454
HapII CCGG 4 cut(s) 705, 812, 1214, 1340
HincII GTYRAC 2 cut(s) 829, 1282
HindII GTYRAC 2 cut(s) 829, 1282
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 4 cut(s) 491, 851, 1127, 1235
HpaII CCGG 4 cut(s) 705, 812, 1214, 1340
HphI GGTGA 2 cut(s) 395, 694
Hpy166II GTNNAC 4 cut(s) 615, 829, 1045, 1282
Hpy188I TCNGA 7 cut(s) 140, 315, 525, 882, 958, 1092, 1450
Hpy188III TCNNGA 7 cut(s) 52, 391, 419, 848, 1131, 1214, 1459
Hpy8I GTNNAC 4 cut(s) 615, 829, 1045, 1282
HpyAV CCTTC 3 cut(s) 687, 1013, 1465
HpyCH4IV ACGT 3 cut(s) 831, 1047, 1491
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 1149, 1166
HpyF3I CTNAG 4 cut(s) 312, 435, 999, 1091
HpySE526I ACGT 3 cut(s) 831, 1047, 1491
Kpn2I TCCGGA 1 cut(s) 1213
LmnI GCTCC 5 cut(s) 21, 244, 379, 859, 1319
Lsp1109I GCAGC 6 cut(s) 114, 387, 590, 634, 644, 990
LweI GCATC 3 cut(s) 157, 771, 1219
MaeI CTAG 3 cut(s) 99, 323, 975
MaeII ACGT 3 cut(s) 831, 1047, 1491
MaeIII GTNAC 1 cut(s) 700
MfeI CAATTG 2 cut(s) 1200, 1385
MflI RGATCY 3 cut(s) 543, 750, 1210
MhlI GDGCHC 2 cut(s) 303, 1185
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 7 cut(s) 686, 786, 866, 894, 1032, 1200, 1385
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 1244
MmeI TCCRAC 3 cut(s) 222, 936, 1202
Mox20I TGGCCA 1 cut(s) 200
Mph1103I ATGCAT 2 cut(s) 187, 1263
MroI TCCGGA 1 cut(s) 1213
MscI TGGCCA 1 cut(s) 200
MseI TTAA 6 cut(s) 11, 533, 804, 1242, 1287, 1425
Msp20I TGGCCA 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 532
MspI CCGG 4 cut(s) 705, 812, 1214, 1340
MspR9I CCNGG 7 cut(s) 59, 114, 706, 812, 1058, 1107, 1341
MunI CAATTG 2 cut(s) 1200, 1385
Mva1269I GAATGC 1 cut(s) 1160
MvaI CCWGG 4 cut(s) 59, 114, 1058, 1107
MwoI GCNNNNNNNGC 3 cut(s) 124, 1149, 1166
NciI CCSGG 3 cut(s) 706, 812, 1341
NcoI CCATGG 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 700
NsiI ATGCAT 2 cut(s) 187, 1263
NspI RCATGY 2 cut(s) 1447, 1470
PceI AGGCCT 2 cut(s) 319, 1454
PciI ACATGT 2 cut(s) 1443, 1466
PcsI WCGNNNNNNNCGW 2 cut(s) 578, 716
PctI GAATGC 1 cut(s) 1160
PfeI GAWTC 3 cut(s) 491, 851, 1127
PflMI CCANNNNNTGG 1 cut(s) 945
PkrI GCNGC 7 cut(s) 129, 274, 402, 580, 634, 649, 1005
PleI GAGTC 1 cut(s) 1243
PpsI GAGTC 1 cut(s) 1243
Ppu21I YACGTR 1 cut(s) 1492
PscI ACATGT 2 cut(s) 1443, 1466
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 4 cut(s) 57, 112, 1056, 1105
PspFI CCCAGC 2 cut(s) 1165, 1322
PspGI CCWGG 4 cut(s) 57, 112, 1056, 1105
PspPI GGNCC 5 cut(s) 95, 116, 212, 515, 991
PstI CTGCAG 2 cut(s) 652, 916
PsuI RGATCY 3 cut(s) 543, 750, 1210
RsaI GTAC 2 cut(s) 834, 1490
RsaNI GTAC 2 cut(s) 833, 1489
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 6 cut(s) 11, 533, 804, 1242, 1287, 1425
SatI GCNGC 7 cut(s) 128, 273, 401, 579, 633, 648, 1004
Sau96I GGNCC 5 cut(s) 95, 116, 212, 515, 991
SchI GAGTC 1 cut(s) 1244
ScrFI CCNGG 7 cut(s) 59, 114, 706, 812, 1058, 1107, 1341
SduI GDGCHC 2 cut(s) 303, 1185
SfaNI GCATC 3 cut(s) 157, 771, 1219
SfcI CTRYAG 3 cut(s) 648, 912, 927
SinI GGWCC 2 cut(s) 212, 515
SmlI CTYRAG 3 cut(s) 532, 626, 755
SmoI CTYRAG 3 cut(s) 532, 626, 755
Sse9I AATT 7 cut(s) 686, 786, 866, 894, 1032, 1200, 1385
SseBI AGGCCT 2 cut(s) 319, 1454
SsiI CCGC 2 cut(s) 272, 1152
SspMI CTAG 3 cut(s) 99, 323, 975
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 2 cut(s) 319, 1454
StyD4I CCNGG 7 cut(s) 57, 112, 704, 810, 1056, 1105, 1339
StyI CCWWGG 3 cut(s) 98, 119, 855
TaiI ACGT 3 cut(s) 834, 1050, 1494
TaqI TCGA 7 cut(s) 420, 458, 489, 572, 942, 1017, 1296
TasI AATT 7 cut(s) 686, 786, 866, 894, 1032, 1200, 1385
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 3 cut(s) 491, 851, 1127
Tru1I TTAA 6 cut(s) 11, 533, 804, 1242, 1287, 1425
Tru9I TTAA 6 cut(s) 11, 533, 804, 1242, 1287, 1425
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 6 cut(s) 127, 400, 578, 632, 647, 1003
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 6 cut(s) 609, 737, 980, 998, 1297, 1449
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 945
Vha464I CTTAAG 1 cut(s) 532
VpaK11BI GGWCC 2 cut(s) 212, 515
XapI RAATTY 3 cut(s) 686, 786, 866
XceI RCATGY 2 cut(s) 1447, 1470
XcmI CCANNNNNNNNNTGG 1 cut(s) 117
XmaJI CCTAGG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 1044
XspI CTAG 3 cut(s) 99, 323, 975
Zsp2I ATGCAT 2 cut(s) 187, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.